FastQCFastQC Report
Fri 10 Feb 2017
SRR2031674_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031674_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619185
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13200.2131834589016207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10920.17636086145497712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10400.045.3262521
GTATCAA17550.034.185461
TCAACGC21550.027.3317174
ATCAACG21750.027.2965723
CAACGCA21850.027.1738435
TGCACCG700.00838872627.1427865
AACGCAG22350.026.5659286
TATCAAC23200.025.8349742
ACGCAGA25100.023.6553177
CGCAGAG25550.023.2386888
CTACACT3300.023.0302454
GTATAGG2601.4457328E-820.1451
AGAGTAC25950.019.95097410-11
GAGTACT13600.019.7318112-13
GCAGAGT29000.019.655129
GTACATG21250.019.494331
ACTATAC2201.3437675E-619.4301973
TATACTG4950.019.191875
CCCCTAT1507.5905526E-419.0461851
GTATAAA3003.596142E-919.0461851
Produced by FastQC (version 0.11.5)