FastQCFastQC Report
Fri 10 Feb 2017
SRR2031674_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031674_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619185
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14560.2351478152733028No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12470.20139376761387956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6620.10691473469157037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12250.044.2241971
GTATCAA20300.035.1144941
GGATCGT550.002580108434.5416376
TCAACGC24150.029.1088664
ATCAACG24350.028.674713
CAACGCA24750.028.5927985
AACGCAG25750.027.851296
TATCAAC24900.027.8505762
ATAAACG908.944422E-426.3881023
ACGTTGT950.001226705824.9972366
ACGCAGA28850.024.693987
CGCAGAG29350.024.2732968
GCAGAGT32100.022.1938089
GTACATG22300.022.1625481
GAGTACT15950.021.58852412-13
TACATGG21900.021.4719642
ACATGGG22100.020.8477953
ATAATAC3650.020.8212993
GTACTTT16550.020.80585914-15
GTGTAGC4450.020.2901291
Produced by FastQC (version 0.11.5)