##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031674_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 619185 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.403121845651945 34.0 31.0 34.0 31.0 34.0 2 32.64092314897809 34.0 31.0 34.0 31.0 34.0 3 32.74811728320292 34.0 31.0 34.0 31.0 34.0 4 36.21735184153363 37.0 35.0 37.0 35.0 37.0 5 35.8634107738398 37.0 35.0 37.0 35.0 37.0 6 35.9258396117477 37.0 36.0 37.0 35.0 37.0 7 35.90972972536479 37.0 36.0 37.0 35.0 37.0 8 36.023154630683884 37.0 36.0 37.0 35.0 37.0 9 37.92062630716183 39.0 38.0 39.0 35.0 39.0 10-11 37.8917165306007 39.0 38.0 39.0 35.0 39.0 12-13 37.90260503726673 39.0 38.0 39.0 35.0 39.0 14-15 39.33764626081058 41.0 39.0 41.0 36.0 41.0 16-17 39.29609809669162 41.0 39.0 41.0 36.0 41.0 18-19 39.30644233952696 41.0 39.0 41.0 36.0 41.0 20-21 39.33198801650556 41.0 39.0 41.0 36.0 41.0 22-23 39.27235559646955 41.0 39.0 41.0 36.0 41.0 24-25 39.20131059376438 41.0 39.0 41.0 36.0 41.0 26-27 39.08269337920008 41.0 39.0 41.0 36.0 41.0 28-29 38.99078304545492 40.0 39.0 41.0 35.0 41.0 30-31 38.87236044154817 40.0 38.0 41.0 35.0 41.0 32-33 38.71476618458134 40.0 38.0 41.0 35.0 41.0 34-35 38.59662944031267 40.0 38.0 41.0 34.5 41.0 36-37 38.46631216841493 40.0 38.0 41.0 34.0 41.0 38-39 38.34063486680071 40.0 38.0 41.0 34.0 41.0 40-41 38.22057704886262 40.0 38.0 41.0 34.0 41.0 42-43 38.06473348030072 40.0 38.0 41.0 33.0 41.0 44-45 37.99295283315972 40.0 38.0 41.0 33.0 41.0 46-47 37.97975080145675 40.0 38.0 41.0 33.0 41.0 48-49 38.06254108222906 40.0 38.0 41.0 33.0 41.0 50-51 37.98325137075349 40.0 38.0 41.0 33.0 41.0 52-53 37.880538934244214 40.0 37.5 41.0 33.0 41.0 54-55 37.767938499802156 40.0 37.0 41.0 33.0 41.0 56-57 37.648951444237184 40.0 37.0 41.0 33.0 41.0 58-59 37.463703093582694 40.0 36.5 41.0 32.0 41.0 60-61 37.18241478717992 39.5 36.0 41.0 32.0 41.0 62-63 36.98876668523947 39.0 35.5 41.0 31.5 41.0 64-65 36.687113705919884 39.0 35.0 41.0 31.0 41.0 66-67 36.39657775947415 38.5 35.0 40.5 31.0 41.0 68-69 36.10161987128241 37.5 35.0 40.0 31.0 41.0 70-71 35.75219199431511 37.0 35.0 39.5 30.0 41.0 72-73 35.37954650064197 36.5 35.0 39.0 30.0 41.0 74-75 35.00755993765999 36.0 34.0 39.0 30.0 40.5 76-77 34.3868674144238 35.0 34.0 37.5 29.0 39.0 78-79 34.34945452489967 35.0 34.0 37.0 29.0 39.0 80-81 34.08748839199916 35.0 34.0 37.0 29.0 39.0 82-83 33.798104766749844 35.0 34.0 36.5 29.0 38.5 84-85 33.52669638314882 35.0 34.0 36.0 29.0 37.0 86-87 33.27456979739496 35.0 34.0 36.0 29.0 37.0 88-89 33.05225255779774 35.0 34.0 35.0 29.0 36.5 90-91 32.894572704442126 35.0 34.0 35.0 28.5 36.0 92-93 32.76708495845345 35.0 33.5 35.0 28.0 36.0 94-95 32.6357825205714 35.0 33.0 35.0 27.0 36.0 96-97 32.536779799252244 35.0 33.0 35.0 27.0 35.5 98-99 32.44437849754112 35.0 33.0 35.0 27.0 35.0 100-101 31.994480647948514 34.5 32.5 35.0 25.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.02619679291598942 1101 2 -0.06455417007236264 1101 3 0.07323642737325997 1101 4 0.07765903282727038 1101 5 -0.013194080775562611 1101 6 -0.008530053205156207 1101 7 -0.04258337826555447 1101 8 0.042459687673137125 1101 9 0.09483180994565998 1101 10-11 -0.040372182563530146 1101 12-13 0.04937528844845929 1101 14-15 0.07890267567215403 1101 16-17 -0.016322571461365953 1101 18-19 -0.03544135782141211 1101 20-21 -0.19783398462055857 1101 22-23 0.1936576193044246 1101 24-25 -0.09661676497596261 1101 26-27 0.020250785609448485 1101 28-29 -0.023747973804908895 1101 30-31 0.008740224681339726 1101 32-33 0.011633118653556096 1101 34-35 -0.006296531983664977 1101 36-37 -0.18340774076327193 1101 38-39 -0.12972421288342417 1101 40-41 -0.11981370534736158 1101 42-43 -0.1454825634968273 1101 44-45 -0.246331407943579 1101 46-47 -0.29042712696884365 1101 48-49 -0.2661722615315085 1101 50-51 -0.15654488514566367 1101 52-53 -0.4015124605784308 1101 54-55 -0.25743640063748074 1101 56-57 -0.17045902463654272 1101 58-59 -0.005276042205757392 1101 60-61 -0.1566072774281153 1101 62-63 -0.3651746360700514 1101 64-65 -0.19414518321963925 1101 66-67 -0.20922828449234032 1101 68-69 -0.5417920722386853 1101 70-71 -0.3404437409664922 1101 72-73 -0.43926417122246875 1101 74-75 -0.5868715172989241 1101 76-77 -0.5513991674349157 1101 78-79 -0.19526915367813302 1101 80-81 -0.25444245873860183 1101 82-83 -0.09620855756357116 1101 84-85 -0.2560375302301665 1101 86-87 -0.27785989162217817 1101 88-89 -0.1624732848089323 1101 90-91 -0.3001866030622722 1101 92-93 -0.6030124268353134 1101 94-95 -0.30693724190251714 1101 96-97 -0.3446766245555395 1101 98-99 -0.6313488062797745 1101 100-101 -0.623154099849252 1102 1 0.0648173119282589 1102 2 0.13284321322370118 1102 3 0.12671509401644698 1102 4 0.10458678291971779 1102 5 0.12550883704302862 1102 6 0.05305933910673133 1102 7 0.10495575783474465 1102 8 0.09028585119072119 1102 9 0.07453914763429736 1102 10-11 0.17162838092587407 1102 12-13 -0.023131711146533007 1102 14-15 0.227762490072827 1102 16-17 0.21918782667911785 1102 18-19 0.21875575781718481 1102 20-21 0.18371878087980775 1102 22-23 0.026389310300920954 1102 24-25 0.1868800389825509 1102 26-27 0.16095409082700485 1102 28-29 -0.21540656965974136 1102 30-31 -0.045043078423134375 1102 32-33 0.11686300472303657 1102 34-35 0.26389135651586315 1102 36-37 0.2980288050466626 1102 38-39 0.010537105329611052 1102 40-41 0.12008662746357146 1102 42-43 -0.15066666605189738 1102 44-45 0.11413100555199662 1102 46-47 0.22383267686641517 1102 48-49 0.27261517969831317 1102 50-51 0.3855072728426805 1102 52-53 0.13353658060911755 1102 54-55 0.22200504048668535 1102 56-57 -0.08815611386156519 1102 58-59 0.2176896729855642 1102 60-61 0.2908931187753794 1102 62-63 0.3435506452344228 1102 64-65 0.30298102079874667 1102 66-67 0.19186699804477314 1102 68-69 0.19891123297887958 1102 70-71 0.3212354574698253 1102 72-73 0.18986999208380695 1102 74-75 0.08928759802270747 1102 76-77 0.24811306204613714 1102 78-79 -0.038397224255177775 1102 80-81 -0.13436638766849995 1102 82-83 -0.3977810451932271 1102 84-85 0.03830292302662741 1102 86-87 -0.02760720184070209 1102 88-89 -0.043858764391245586 1102 90-91 -0.10269941364178692 1102 92-93 -0.051560561157096174 1102 94-95 0.039038211090137054 1102 96-97 -0.031781521630676934 1102 98-99 -0.06492397048410936 1102 100-101 -0.35346454729505794 1103 1 0.022248504588809226 1103 2 0.07467807560902173 1103 3 0.11689023997140424 1103 4 0.019749418449329426 1103 5 -0.025300170463225413 1103 6 -0.09054366339535136 1103 7 0.04854207976968894 1103 8 -0.17088178850902835 1103 9 0.10159502753421634 1103 10-11 -0.016419992718830656 1103 12-13 0.03316520294855252 1103 14-15 -0.02952691776703631 1103 16-17 0.1141502954030571 1103 18-19 0.0890726885928288 1103 20-21 -0.08855811653470624 1103 22-23 -0.10396932189257058 1103 24-25 -0.07955865993321254 1103 26-27 -0.09339953719634764 1103 28-29 0.11271686653709168 1103 30-31 -0.06554349543731064 1103 32-33 0.04812238754205822 1103 34-35 -0.06979504882190923 1103 36-37 0.00913806276141571 1103 38-39 0.12969473668908194 1103 40-41 0.11011998859785166 1103 42-43 -0.1901745893212876 1103 44-45 0.22363892882138714 1103 46-47 -0.19827741487670636 1103 48-49 -0.37181351087716763 1103 50-51 -0.2757771308270449 1103 52-53 -0.26348593815652066 1103 54-55 -0.05118094783691873 1103 56-57 -0.18914860760635577 1103 58-59 -0.1072477748876608 1103 60-61 -0.2974221439435567 1103 62-63 -0.4483259185687416 1103 64-65 -0.33213157302945007 1103 66-67 -0.1969320011211977 1103 68-69 -0.1518060314081069 1103 70-71 -0.10382709465694973 1103 72-73 -0.28699406129400984 1103 74-75 -0.21939463717329488 1103 76-77 -0.12830895630248307 1103 78-79 0.06263697090745524 1103 80-81 0.06637589756919482 1103 82-83 -0.15381107021407558 1103 84-85 -0.44379882843292506 1103 86-87 -0.288674758137617 1103 88-89 -0.18196552002093824 1103 90-91 0.03131226275522181 1103 92-93 0.022267629001362366 1103 94-95 -0.2749567847397216 1103 96-97 -0.1543503289701249 1103 98-99 -0.14067876614716113 1103 100-101 0.05756130758400957 1104 1 0.20519932426094556 1104 2 0.18895858562723333 1104 3 0.11665510170347915 1104 4 0.15681184600355635 1104 5 0.1747693774751795 1104 6 0.17330465586280752 1104 7 0.2943509885294233 1104 8 0.08206406345321682 1104 9 0.17090451586009436 1104 10-11 0.150059556875469 1104 12-13 0.14512082474023202 1104 14-15 0.12137389362558082 1104 16-17 0.30804663585677616 1104 18-19 0.08166914495977551 1104 20-21 0.11413438222336225 1104 22-23 0.07036417703109521 1104 24-25 0.1201532128934133 1104 26-27 0.3720367933219535 1104 28-29 0.23044827902260323 1104 30-31 0.055284456210308974 1104 32-33 -0.10799286332728997 1104 34-35 0.12887857263859104 1104 36-37 0.198553528898195 1104 38-39 -0.07338027573019446 1104 40-41 -0.044913957419701944 1104 42-43 -0.03460744244266323 1104 44-45 -0.039997434814978305 1104 46-47 0.2622143884019792 1104 48-49 0.096752476869149 1104 50-51 0.21016093838971273 1104 52-53 0.3169793706694435 1104 54-55 0.0057904050360733095 1104 56-57 -0.018824050560489525 1104 58-59 0.006446545364035217 1104 60-61 0.07858216140501639 1104 62-63 0.08011587643042617 1104 64-65 0.25789988913316364 1104 66-67 2.976726658516782E-4 1104 68-69 0.05043109394376444 1104 70-71 0.020456727167854183 1104 72-73 0.008412462964976442 1104 74-75 -0.21694224439302445 1104 76-77 0.027119094699337154 1104 78-79 0.1600636971866365 1104 80-81 0.17515384093730546 1104 82-83 0.0870036342071927 1104 84-85 0.33425051748527324 1104 86-87 -0.036295225102342954 1104 88-89 -0.14653084540593397 1104 90-91 -0.060686412522144906 1104 92-93 0.046589536601963744 1104 94-95 -0.22044776006675448 1104 96-97 -0.24458712314135056 1104 98-99 0.05760406213566682 1104 100-101 0.2435324948666704 1105 1 -0.01950588137609799 1105 2 0.018888601924807347 1105 3 0.009139043799159197 1105 4 0.13738897187835164 1105 5 -3.240939081976535E-4 1105 6 -0.11344637471911767 1105 7 0.022258188222643582 1105 8 -0.013593112272985763 1105 9 0.1278789190812617 1105 10-11 0.16262306948212313 1105 12-13 0.04321304983849927 1105 14-15 0.15224341716119483 1105 16-17 0.0422523687682812 1105 18-19 0.09046024840143474 1105 20-21 0.1385232231782041 1105 22-23 0.030799418139324075 1105 24-25 0.060202105617022994 1105 26-27 0.1925813240476728 1105 28-29 0.003610008482858973 1105 30-31 0.07534964650846376 1105 32-33 0.2089038867446007 1105 34-35 0.20224641848270863 1105 36-37 0.17355592560033273 1105 38-39 0.06699936188844191 1105 40-41 0.057089685567540016 1105 42-43 0.18328633571858433 1105 44-45 0.24859905641309155 1105 46-47 0.43382784828119014 1105 48-49 0.2214241286762615 1105 50-51 0.18895971127821554 1105 52-53 0.2593051304240248 1105 54-55 0.26277439506579015 1105 56-57 0.23249413561533316 1105 58-59 0.3579037402638505 1105 60-61 0.33323176355245465 1105 62-63 0.15818125846474373 1105 64-65 0.12743780496098367 1105 66-67 0.2125735810159597 1105 68-69 0.20509986970831307 1105 70-71 0.10558279370030732 1105 72-73 0.21201710298031173 1105 74-75 0.08424172646307682 1105 76-77 0.15221735948698978 1105 78-79 0.029750208547014267 1105 80-81 -0.1737676431006676 1105 82-83 -0.033013622048201796 1105 84-85 0.07202254317792267 1105 86-87 0.16197914935839464 1105 88-89 0.25299460757076986 1105 90-91 0.1798130602033865 1105 92-93 0.20199649662496455 1105 94-95 0.04607989787590583 1105 96-97 0.24705317980180297 1105 98-99 0.15468008552747392 1105 100-101 -0.14237489656272473 1106 1 0.1556273441792584 1106 2 0.07870537936328503 1106 3 0.1811347326800643 1106 4 0.17520706660322105 1106 5 0.10238831852219477 1106 6 0.012360463194781346 1106 7 0.2496218191429449 1106 8 0.0966577709076617 1106 9 0.34932074891181486 1106 10-11 0.192109327790007 1106 12-13 0.16850494852720033 1106 14-15 0.143845716424714 1106 16-17 0.15226075149985974 1106 18-19 0.038430429827705836 1106 20-21 0.07012013354285784 1106 22-23 0.124653079596726 1106 24-25 0.0823774244602049 1106 26-27 0.13632121986228896 1106 28-29 0.15052636079709458 1106 30-31 0.017146544897777005 1106 32-33 0.04379101925164264 1106 34-35 -0.19195452756842002 1106 36-37 -0.1378026756810229 1106 38-39 0.060709319376016424 1106 40-41 -0.09962248735623547 1106 42-43 -0.1468795226132542 1106 44-45 -0.028052042323508886 1106 46-47 0.0028147352939384973 1106 48-49 0.09023737339214222 1106 50-51 -0.06772252331851547 1106 52-53 -0.20173911842334746 1106 54-55 -0.10831715633831607 1106 56-57 -0.05825192749465913 1106 58-59 -0.12440570228449843 1106 60-61 -0.15552639464166162 1106 62-63 -0.026181953318975104 1106 64-65 -0.2090630033738492 1106 66-67 -0.0075928760824126584 1106 68-69 -0.00166020453873017 1106 70-71 -0.24008213654339272 1106 72-73 0.074926509602669 1106 74-75 0.19192090735044331 1106 76-77 0.06643507100127266 1106 78-79 0.02160997742313242 1106 80-81 0.0733942034953472 1106 82-83 0.0672438475643986 1106 84-85 -0.05534707540779493 1106 86-87 0.10632679321205529 1106 88-89 0.12378378187170824 1106 90-91 0.11196382961838935 1106 92-93 0.027048888697301265 1106 94-95 0.13235317492519272 1106 96-97 -0.09557899791210644 1106 98-99 0.16416454753574072 1106 100-101 0.16842385800286763 1107 1 0.19037858588962564 1107 2 0.15744230325129394 1107 3 0.2313470027208524 1107 4 0.09117587816847106 1107 5 0.221070856883415 1107 6 0.2420949175359155 1107 7 0.1352235284023635 1107 8 0.16672353299873066 1107 9 0.08645829434870222 1107 10-11 0.1464698816344594 1107 12-13 0.01749631898698567 1107 14-15 0.11291949760620668 1107 16-17 0.13660410175929627 1107 18-19 -0.054511938162526974 1107 20-21 0.043016162844445205 1107 22-23 0.16092350885391227 1107 24-25 0.23390771109413322 1107 26-27 0.16416882939640942 1107 28-29 0.0123177534550436 1107 30-31 -9.019581128413279E-4 1107 32-33 0.15458210668469974 1107 34-35 0.15513473683957812 1107 36-37 0.14118536505262824 1107 38-39 0.2861692377977292 1107 40-41 0.1749758644293351 1107 42-43 -0.09979025820524612 1107 44-45 0.12341719933136375 1107 46-47 0.28928356073304684 1107 48-49 0.12610962289961947 1107 50-51 0.06780010494531297 1107 52-53 -0.132436418570407 1107 54-55 0.046343072857830236 1107 56-57 0.06608420835816986 1107 58-59 -0.03437268250037562 1107 60-61 -0.08476139006328509 1107 62-63 -0.21165186091907628 1107 64-65 -0.3301360076192452 1107 66-67 -0.05569770696007481 1107 68-69 -0.017386223573673476 1107 70-71 -0.019385113873511273 1107 72-73 -0.005013277849812425 1107 74-75 -0.08445746052879599 1107 76-77 -0.09489432215613647 1107 78-79 -0.12300974010511112 1107 80-81 0.06679718360023656 1107 82-83 -0.06433583000712417 1107 84-85 -0.19288973752383498 1107 86-87 -0.15235546767198827 1107 88-89 -0.10145554219388941 1107 90-91 0.062162225800300064 1107 92-93 0.048402144891973364 1107 94-95 0.07845045842362453 1107 96-97 0.11003991493231524 1107 98-99 0.030503791132957758 1107 100-101 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-1.5474903439022825 2316 76-77 -1.3426120686164182 2316 78-79 -1.3962189769336035 2316 80-81 -1.4646229952835554 2316 82-83 -1.6161582223853088 2316 84-85 -1.8662320920569861 2316 86-87 -1.487798881646043 2316 88-89 -1.3771445088264471 2316 90-91 -1.091996877360419 2316 92-93 -0.9481863013713792 2316 94-95 -1.1280162016421684 2316 96-97 -0.790940315438327 2316 98-99 -1.0980868114171827 2316 100-101 -1.2598356662135082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 10.0 14 38.0 15 79.0 16 219.0 17 406.0 18 690.0 19 1115.0 20 1580.0 21 2415.0 22 3043.0 23 3412.0 24 3200.0 25 3343.0 26 3731.0 27 4507.0 28 5397.0 29 6826.0 30 8614.0 31 10787.0 32 13639.0 33 18387.0 34 25851.0 35 38893.0 36 67833.0 37 136567.0 38 194978.0 39 63545.0 40 80.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.53360692819751 19.948781748852838 15.120209397014154 22.3974019259355 2 15.150433559386089 20.595726262441918 40.91802505584025 23.33581512233174 3 18.393108337922715 25.672700962397222 30.61679664977882 25.317394049901242 4 12.447163676713577 17.71957055799357 39.28938880265663 30.54387696263622 5 13.054418307937047 38.05534694800423 35.45919232539548 13.431042418663242 6 29.98312297617029 38.30648352269515 18.310359585584276 13.40003391555028 7 26.98870289170442 32.92263216970696 22.34808659770505 17.74057834088358 8 25.237852984164665 36.53689931119132 20.357082293660213 17.868165410983792 9 25.533402779460097 16.863619112220096 20.855963888014085 36.74701422030572 10-11 24.231207151336033 26.96560801699008 28.85179712040828 19.951387711265614 12-13 25.125366409069986 24.09271865436017 29.106002244886426 21.675912691683422 14-15 22.245047925902597 25.858992062146207 26.646236585188593 25.2497234267626 16-17 21.793244345389503 29.154776036241188 27.221831924222972 21.830147694146337 18-19 21.628269418671316 28.179623214386652 29.13814126634205 21.053966100599983 20-21 22.508135694501643 27.095213869845036 29.23504283857006 21.16160759708326 22-23 22.21467632684835 27.10635459499268 28.848998827470147 21.82997025068882 24-25 21.94053473517608 27.250821644581187 28.921566252412447 21.887077367830294 26-27 22.001501974369532 27.425244474591597 28.719445723006853 21.85380782803201 28-29 21.975958381491687 27.44157796241508 28.763519674857424 21.81894398123581 30-31 22.133513604321333 27.22883431222418 28.83124309445812 21.806408988996363 32-33 21.9686361911222 27.41813835929488 28.722191267553317 21.891034182029603 34-35 22.231481705790674 27.43218908726794 28.680119834944325 21.656209371997058 36-37 22.309325968813845 27.385595581288307 28.52992239799091 21.77515605190694 38-39 22.189168019251113 27.327939145812643 28.664696334698032 21.818196500238216 40-41 22.29810153669743 27.264064859452347 28.54486139037606 21.892972213474163 42-43 22.241979376115378 27.368718557458592 28.404838618506584 21.984463447919442 44-45 22.231320203170295 27.51269814352738 28.26320082043331 21.992780832869013 46-47 22.249973755824186 27.323094067201243 28.209339696536578 22.217592480437993 48-49 22.164017005437184 27.389723552595125 28.180300285066444 22.26595915690125 50-51 22.22740877014745 27.627466593875532 28.06049847930056 22.08462615667646 52-53 22.332340092217994 27.692127554769574 27.950774001308172 22.024758351704257 54-55 22.306015774075572 27.664550159444623 27.956387515433708 22.0730465510461 56-57 22.25409207264388 27.53240146321374 27.93187819472371 22.281628269418672 58-59 22.404289509597294 27.538296308857614 27.988565614477096 22.068848567068 60-61 22.24819722700001 27.650702132642103 28.050017361531694 22.0510832788262 62-63 22.27629868294613 27.723782068364063 28.004312119964148 21.995607128725663 64-65 22.298747547178955 27.615494561399256 27.869538183257024 22.216219708164765 66-67 22.26636627179276 27.54451415974224 27.951581514410073 22.23753805405493 68-69 22.323053691546146 27.53740804444552 27.932604956515423 22.206933307492914 70-71 22.25094277154647 27.430331807133573 27.92671011087155 22.392015310448414 72-73 22.18295016836648 27.63091806164555 27.74130510267529 22.44482666731268 74-75 22.16268158950879 27.73201870200344 27.648360344646594 22.456939363841176 76-77 22.145966068299458 27.480962878622705 27.749622487624865 22.623448565452975 78-79 22.17587986769684 27.614893149693792 27.643398408227604 22.565828574381765 80-81 22.234659340073605 27.54296660795378 27.59796451221866 22.624409539753955 82-83 22.26938361513073 27.585054037750112 27.634476593332312 22.51108575378684 84-85 22.170191461356463 27.642627001623097 27.61824010594572 22.56894143107472 86-87 22.108416709061103 27.54507941891357 27.703513489506363 22.642990382518956 88-89 22.218076988299135 27.400534573673458 27.583759296494588 22.79762914153282 90-91 22.17237174673159 27.55363905779371 27.52933291342652 22.744656282048176 92-93 22.221468543327116 27.485969459854488 27.56470198728974 22.727860009528655 94-95 22.084756575175433 27.43937595387485 27.675089028319483 22.800778442630232 96-97 22.05883540460444 27.358786146305224 27.7089238272891 22.87345462180124 98-99 22.013210914347088 27.405379652284857 27.555819343168842 23.025590090199213 100-101 22.03372174713535 27.50454226119819 27.457060490806462 23.00467550086 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7.0 1 6.5 2 13.5 3 34.5 4 32.5 5 28.5 6 53.5 7 106.0 8 201.5 9 294.5 10 309.0 11 285.5 12 301.0 13 329.5 14 361.5 15 394.5 16 452.0 17 484.0 18 526.0 19 587.5 20 638.5 21 741.5 22 854.5 23 1042.5 24 1322.0 25 1687.5 26 2231.5 27 2923.5 28 3897.5 29 5210.5 30 7032.0 31 9090.0 32 11399.5 33 14107.5 34 16498.0 35 18211.0 36 19472.0 37 20192.5 38 20294.0 39 20234.5 40 20457.5 41 20724.5 42 20787.5 43 21322.0 44 21973.5 45 22527.5 46 23236.5 47 23509.5 48 23730.0 49 24036.5 50 23617.5 51 22946.0 52 22189.0 53 21233.5 54 20023.0 55 18496.0 56 16667.0 57 14583.0 58 12697.0 59 10653.0 60 8625.5 61 6836.0 62 5162.5 63 3657.5 64 2422.5 65 1588.5 66 1060.5 67 759.0 68 524.0 69 350.5 70 241.0 71 191.0 72 155.5 73 103.0 74 75.0 75 52.5 76 35.5 77 17.0 78 9.5 79 8.5 80 5.0 81 3.5 82 3.0 83 1.0 84 0.0 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04279819440070415 2 0.0012920209630401254 3 3.2300524076003134E-4 4 0.009044146741280876 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0017765288241801723 24-25 0.0 26-27 0.0 28-29 0.021560599820732093 30-31 0.018895806584461834 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.033350291108473235 50-51 0.010013162463560972 52-53 0.0 54-55 7.267617917100705E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.6150262038001567E-4 80-81 0.011385934736791104 82-83 0.00557184040311054 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 619185.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.88042812110712 #Duplication Level Percentage of deduplicated Percentage of total 1 79.5849837514763 58.00187687818945 2 13.314691099157706 19.407607752138155 3 3.9016635905054975 8.530647385817296 4 1.5253229936571955 4.446647712028206 5 0.6943196507898647 2.530115670123146 6 0.3616421089469462 1.5813979036004098 7 0.18968389168522767 0.9676970263587971 8 0.12578375269331918 0.7333738997574849 9 0.08018301413102333 0.5259395158118786 >10 0.21484219301940252 2.3463838440507203 >50 0.005329523707658208 0.2712446764912457 >100 8.882458456448715E-4 0.11247834391441346 >500 2.2206146138590579E-4 0.10713764556252324 >1k 4.4412292277181157E-4 0.43745174615634486 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1456 0.2351478152733028 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1247 0.20139376761387956 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 662 0.10691473469157037 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.6150262038001567E-4 0.0 6 0.0 0.0 0.0 1.6150262038001567E-4 0.0 7 0.0 0.0 0.0 1.6150262038001567E-4 0.0 8 0.0 0.0 0.0 1.6150262038001567E-4 0.0 9 0.0 0.0 0.0 1.6150262038001567E-4 0.0 10-11 0.0 0.0 0.0 3.2300524076003134E-4 0.0 12-13 0.0 0.0 0.0 3.2300524076003134E-4 0.0 14-15 0.0 0.0 0.0 4.84507861140047E-4 0.0 16-17 0.0 0.0 0.0 0.0020187827547501957 0.0 18-19 0.0 0.0 0.0 0.002503290615890243 0.0 20-21 0.0 0.0 0.0 0.0035530576483603447 0.0 22-23 0.0 0.0 0.0 0.006298602194820611 0.0 24-25 0.0 0.0 0.0 0.009851659843180956 0.0 26-27 0.0 0.0 0.0 0.01082067556546105 0.0 28-29 0.0 0.0 0.0 0.012354950459071198 0.0 30-31 0.0 0.0 0.0 0.014938992385151449 0.0 32-33 0.0 0.0 0.0 0.018411298723321784 0.0 34-35 0.0 0.0 0.0 0.02357938257548229 0.0 36-37 0.0 0.0 0.0 0.0319775188352431 0.0 38-39 0.0 0.0 0.0 0.04627050073887449 0.0 40-41 0.0 0.0 0.0 0.06411654029086622 0.0 42-43 0.0 0.0 0.0 0.08058980756962782 0.0 44-45 0.0 0.0 0.0 0.10215040739035991 0.0 46-47 0.0 0.0 0.0 0.13178613823009278 0.0 48-49 0.0 0.0 0.0 0.16158337169020567 0.0 50-51 0.0 0.0 0.0 0.19598342983114903 0.0 52-53 0.0 0.0 0.0 0.22933372093962223 0.0 54-55 0.0 0.0 0.0 0.2637337790805656 0.0 56-57 0.0 0.0 0.0 0.3074202378933598 0.0 58-59 0.0 0.0 0.0 0.3536907386322343 0.0 60-61 0.0 0.0 0.0 0.40666359811687947 0.0 62-63 0.0 0.0 0.0 0.46407777966197505 0.0 64-65 0.0 0.0 0.0 0.5277098120917012 0.0 66-67 0.0 0.0 0.0 0.6032122871193585 0.0 68-69 0.0 0.0 0.0 0.6900199455736169 0.0 70-71 0.0 0.0 0.0 0.7874060256627664 0.0 72-73 0.0 0.0 0.0 0.8892334278123664 0.0 74-75 0.0 0.0 0.0 0.9967134216752667 0.0 76-77 0.0 0.0 0.0 1.1170328738583784 0.0 78-79 0.0 0.0 0.0 1.2501110330515113 0.0 80-81 0.0 0.0 0.0 1.4083028497137366 0.0 82-83 0.0 0.0 0.0 1.5722280093994525 0.0 84-85 0.0 0.0 0.0 1.7542414625677303 0.0 86-87 0.0 0.0 0.0 1.9453798137874787 0.0 88-89 0.0 0.0 0.0 2.16252008688841 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1225 0.0 44.224197 1 GTATCAA 2030 0.0 35.114494 1 GGATCGT 55 0.0025801084 34.541637 6 TCAACGC 2415 0.0 29.108866 4 ATCAACG 2435 0.0 28.67471 3 CAACGCA 2475 0.0 28.592798 5 AACGCAG 2575 0.0 27.85129 6 TATCAAC 2490 0.0 27.850576 2 ATAAACG 90 8.944422E-4 26.388102 3 ACGTTGT 95 0.0012267058 24.997236 6 ACGCAGA 2885 0.0 24.69398 7 CGCAGAG 2935 0.0 24.273296 8 GCAGAGT 3210 0.0 22.193808 9 GTACATG 2230 0.0 22.162548 1 GAGTACT 1595 0.0 21.588524 12-13 TACATGG 2190 0.0 21.471964 2 ACATGGG 2210 0.0 20.847795 3 ATAATAC 365 0.0 20.821299 3 GTACTTT 1655 0.0 20.805859 14-15 GTGTAGC 445 0.0 20.290129 1 >>END_MODULE