FastQCFastQC Report
Fri 10 Feb 2017
SRR2031672_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031672_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009903
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19900.19704862744243756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17210.1704124059439372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17800.037.706221
GTATCAA26800.034.9901121
CTATACG550.00257404234.560314
TCAACGC31350.029.7097424
ATCAACG31750.029.3339863
CAACGCA32050.029.2091245
AACGCAG32650.028.6609756
TATCAAC33850.027.3982562
GTACATG34450.025.5620921
ACGCAGA37500.025.0808267
ACTCCGT950.001226214825.0008226
TACATGG34300.024.6815742
CGCAGAG38150.024.6534988
ACATGGG34000.024.3180713
GCAGAGT42750.021.8896089
GAGTACT21100.021.72249212-13
CATGGGG17950.021.7084944
GTACCTG9000.020.6269971
TACCTGG8900.019.7723672
CTACACT6300.019.6116054
Produced by FastQC (version 0.11.5)