FastQCFastQC Report
Fri 10 Feb 2017
SRR2031672_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031672_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009903
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20310.2011084232842164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19470.1927907927791085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10160.10060372134749575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19300.044.0887341
GTATCAA28150.031.4098951
ACGATAC650.00583988629.2347183
TCAACGC33050.026.304614
ATCAACG33350.025.9255393
CAACGCA34250.025.2380355
AACGCAG35200.024.8267526
GTACATG32800.024.058371
TATCAAC36500.023.9484392
TACATGG34050.022.8812082
ACGCAGA38300.022.6932667
ACATGGG33400.022.4730933
CTACACT4450.022.4187564
CGCAGAG39200.021.929938
GAGTACT23300.021.8108512-13
GAGTACA25900.020.1894681
AAGACGG1908.257648E-619.997755
TACCGTG1908.257648E-619.997757
GTATAGG4300.019.8992181
GTGTAGC7150.019.2807481
Produced by FastQC (version 0.11.5)