##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031672_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1009903 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45680327714642 34.0 31.0 34.0 31.0 34.0 2 32.67736604406562 34.0 31.0 34.0 31.0 34.0 3 32.78667852259078 34.0 31.0 34.0 31.0 34.0 4 36.23498989506913 37.0 37.0 37.0 35.0 37.0 5 35.89771591925165 37.0 35.0 37.0 35.0 37.0 6 35.964486688325515 37.0 36.0 37.0 35.0 37.0 7 35.9415151752198 37.0 36.0 37.0 35.0 37.0 8 36.06297139428242 37.0 37.0 37.0 35.0 37.0 9 37.964171806599246 39.0 39.0 39.0 35.0 39.0 10-11 37.94103889185397 39.0 38.0 39.0 35.0 39.0 12-13 37.95270139805506 39.0 38.0 39.0 35.0 39.0 14-15 39.41105581427127 41.0 39.0 41.0 36.0 41.0 16-17 39.377940752725756 41.0 39.0 41.0 36.0 41.0 18-19 39.388782883108576 41.0 39.0 41.0 36.0 41.0 20-21 39.41331642741926 41.0 39.0 41.0 36.0 41.0 22-23 39.3468580645864 41.0 39.0 41.0 36.0 41.0 24-25 39.28180429209538 41.0 39.0 41.0 36.0 41.0 26-27 39.16611050764281 41.0 39.0 41.0 36.0 41.0 28-29 39.080817662686414 40.5 39.0 41.0 36.0 41.0 30-31 38.97226961401243 40.0 38.5 41.0 35.0 41.0 32-33 38.809977294849105 40.0 38.0 41.0 35.0 41.0 34-35 38.68660356489683 40.0 38.0 41.0 35.0 41.0 36-37 38.54580093335697 40.0 38.0 41.0 34.5 41.0 38-39 38.42619489198468 40.0 38.0 41.0 34.0 41.0 40-41 38.311006601624115 40.0 38.0 41.0 34.0 41.0 42-43 38.150088176785296 40.0 38.0 41.0 33.5 41.0 44-45 38.07329416785572 40.0 38.0 41.0 33.0 41.0 46-47 38.05916112735579 40.0 38.0 41.0 33.0 41.0 48-49 38.14169479643094 40.0 38.0 41.0 33.0 41.0 50-51 38.05957354320167 40.0 38.0 41.0 33.0 41.0 52-53 37.96235133473215 40.0 38.0 41.0 33.0 41.0 54-55 37.8504410819653 40.0 37.0 41.0 33.0 41.0 56-57 37.73740844417731 40.0 37.0 41.0 33.0 41.0 58-59 37.54421464239635 40.0 36.5 41.0 33.0 41.0 60-61 37.267030596007736 39.5 36.0 41.0 32.0 41.0 62-63 37.07725494428673 39.0 35.5 41.0 32.0 41.0 64-65 36.79292961799301 39.0 35.0 41.0 31.0 41.0 66-67 36.51189272633114 38.5 35.0 41.0 31.0 41.0 68-69 36.21932452918746 37.5 35.0 40.0 31.0 41.0 70-71 35.88752583168879 37.0 35.0 40.0 31.0 41.0 72-73 35.52548561594529 36.5 35.0 39.0 30.0 41.0 74-75 35.16799385683575 36.0 35.0 39.0 30.0 41.0 76-77 34.54620542764998 35.0 34.0 38.0 29.0 40.0 78-79 34.50175462395893 35.0 34.0 37.0 30.0 39.0 80-81 34.231767308345454 35.0 34.0 37.0 29.5 39.0 82-83 33.942192963086555 35.0 34.0 36.5 29.0 39.0 84-85 33.66443707960072 35.0 34.0 36.0 29.0 37.0 86-87 33.40960220932109 35.0 34.0 36.0 29.0 37.0 88-89 33.17423703068513 35.0 34.0 35.5 29.0 37.0 90-91 33.01023316100655 35.0 34.0 35.0 29.0 36.0 92-93 32.87395225085973 35.0 34.0 35.0 29.0 36.0 94-95 32.74462299844639 35.0 34.0 35.0 28.5 36.0 96-97 32.64289887246597 35.0 33.5 35.0 27.0 36.0 98-99 32.54938543602702 35.0 33.0 35.0 27.0 35.0 100-101 32.11041951553763 34.5 32.5 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27229761334132974 1101 2 0.2402566251982421 1101 3 0.27753193055060876 1101 4 0.18273425506025376 1101 5 0.3179422462969086 1101 6 0.14382193048742664 1101 7 0.3311688987878654 1101 8 0.2975203486781979 1101 9 0.2438289250935739 1101 10-11 0.12451458804957127 1101 12-13 0.06294662395038841 1101 14-15 0.17842845866591261 1101 16-17 0.12598282331465782 1101 18-19 0.1108098164030622 1101 20-21 0.13961208226905342 1101 22-23 0.16494342512913107 1101 24-25 0.08865858378167246 1101 26-27 0.21181164062387126 1101 28-29 0.06233579392229416 1101 30-31 0.029752914873007796 1101 32-33 0.03114179490442126 1101 34-35 -0.03223885193921916 1101 36-37 0.046830531737931835 1101 38-39 0.09438269856509152 1101 40-41 0.19379992327508688 1101 42-43 0.04961828968072979 1101 44-45 0.09123999810565664 1101 46-47 0.10509637365822755 1101 48-49 0.11273791903565922 1101 50-51 0.3246946507171984 1101 52-53 0.24379784531407722 1101 54-55 0.28771583041463344 1101 56-57 0.09951509697921779 1101 58-59 0.11940910954402995 1101 60-61 -0.09008878605943238 1101 62-63 -0.03662549944207427 1101 64-65 0.029056662199636207 1101 66-67 0.1199150366279369 1101 68-69 -0.021836837241629325 1101 70-71 -0.006410023807241316 1101 72-73 -0.07915752385186181 1101 74-75 -0.1539279495932817 1101 76-77 -0.047061067767984355 1101 78-79 0.07439841847934048 1101 80-81 0.06431396889797014 1101 82-83 0.0549487725919775 1101 84-85 0.1064416145867284 1101 86-87 0.23195422456927162 1101 88-89 0.11573404927451492 1101 90-91 0.206759916060399 1101 92-93 0.025090437692249168 1101 94-95 0.0937201727794914 1101 96-97 0.04458071347697512 1101 98-99 0.08379645971177041 1101 100-101 0.054707315935473844 1102 1 0.15170947506451427 1102 2 0.19668227502011604 1102 3 0.15062699586265182 1102 4 0.1496631594891582 1102 5 0.020404092626677084 1102 6 0.06540074475491764 1102 7 0.12286580251306845 1102 8 0.06006323032485028 1102 9 0.14205373487498463 1102 10-11 0.21324111309684923 1102 12-13 0.12136462104763268 1102 14-15 0.20659565310669592 1102 16-17 0.14640520753420816 1102 18-19 0.18197960573322547 1102 20-21 0.13845757639755618 1102 22-23 0.006385787797910325 1102 24-25 0.12701979018532938 1102 26-27 0.11374049007631015 1102 28-29 -0.24055569352108108 1102 30-31 -0.23001090621156806 1102 32-33 0.2115924370295943 1102 34-35 0.05817819956273951 1102 36-37 0.22538173682215046 1102 38-39 0.11405740279168697 1102 40-41 -0.03980884217330072 1102 42-43 0.20834635406917812 1102 44-45 0.13480335298977053 1102 46-47 0.21743804142442258 1102 48-49 0.17559801491461968 1102 50-51 0.21627692850230318 1102 52-53 0.2658255424597016 1102 54-55 -0.012179439409884196 1102 56-57 -0.04292618815074434 1102 58-59 -0.13178937750351594 1102 60-61 0.3628747205597307 1102 62-63 0.2226352869413546 1102 64-65 0.2283821009118725 1102 66-67 0.23865459109862996 1102 68-69 0.08039878091379649 1102 70-71 -0.06416830326362799 1102 72-73 -0.006772754533130865 1102 74-75 0.14265636232121892 1102 76-77 0.07205919875794109 1102 78-79 -0.06497792844417205 1102 80-81 -0.12892145134216548 1102 82-83 -0.5951273698190676 1102 84-85 -0.10128697757582472 1102 86-87 -0.13387432581117054 1102 88-89 -0.14725963942672138 1102 90-91 0.07139685145204311 1102 92-93 -0.023145917147878947 1102 94-95 -0.029497215178459157 1102 96-97 -0.04411969774702129 1102 98-99 0.009365191294655517 1102 100-101 -0.09030686799380305 1103 1 0.058715167784491484 1103 2 0.047609125946969755 1103 3 0.046231391467046024 1103 4 -0.06315576761548414 1103 5 -0.13305356083097308 1103 6 -5.888212346505384E-4 1103 7 0.12338908875064192 1103 8 0.01213496811087822 1103 9 0.0954178310962277 1103 10-11 0.021377421233161442 1103 12-13 -0.05868945186357877 1103 14-15 0.03610344690020639 1103 16-17 0.0680193077197444 1103 18-19 0.1493772874166197 1103 20-21 -0.053548829894559447 1103 22-23 -0.12002748147250486 1103 24-25 0.06383694128819428 1103 26-27 0.07111644745226897 1103 28-29 0.1202779173125279 1103 30-31 0.052107768764251716 1103 32-33 -0.10546748717318621 1103 34-35 -0.05325401901233562 1103 36-37 -0.07440577725108 1103 38-39 -0.06333266745552635 1103 40-41 -0.039174555824729396 1103 42-43 0.0066948309890833 1103 44-45 0.15221847754774842 1103 46-47 -0.01982073154864139 1103 48-49 -0.004523450921134042 1103 50-51 -0.12074319423210511 1103 52-53 -0.06384478721063402 1103 54-55 -0.12912598492786032 1103 56-57 0.06988956752215358 1103 58-59 0.18471951043395052 1103 60-61 0.013295728123594586 1103 62-63 -0.1271974200342072 1103 64-65 -0.024603009216093596 1103 66-67 -0.16241259568284505 1103 68-69 -0.07315005120646134 1103 70-71 0.055616673664204086 1103 72-73 0.02272552496514635 1103 74-75 -0.22230010977810366 1103 76-77 -0.20692768737180245 1103 78-79 0.03280127647789044 1103 80-81 0.04253804154589602 1103 82-83 -0.010132999110410879 1103 84-85 -0.19512964284706413 1103 86-87 -0.3274505908160066 1103 88-89 -0.08347818278812014 1103 90-91 0.08036407470498119 1103 92-93 -0.11200150600346603 1103 94-95 -0.11279883419436487 1103 96-97 0.08521921730617521 1103 98-99 -0.011023150522255776 1103 100-101 0.029723894894097214 1104 1 0.012412530398442811 1104 2 0.012986775888379043 1104 3 0.07723171105796922 1104 4 0.04948481434436758 1104 5 0.029884545060149037 1104 6 -0.105521174489148 1104 7 -0.020655106108506516 1104 8 0.05233494071737255 1104 9 0.07863169829858663 1104 10-11 -0.03377864566787281 1104 12-13 -0.016735533831045757 1104 14-15 0.033988059358158296 1104 16-17 0.057529877048146716 1104 18-19 0.17567885949959106 1104 20-21 0.05957113845072115 1104 22-23 0.1146131309245817 1104 24-25 0.18620221849527496 1104 26-27 0.07359327711030517 1104 28-29 0.0017629498042666114 1104 30-31 -0.05999635700238315 1104 32-33 0.1442319451724572 1104 34-35 0.3437768291210972 1104 36-37 0.2492979771813424 1104 38-39 0.09567142635201975 1104 40-41 -0.042785172330837895 1104 42-43 0.030454894298522106 1104 44-45 0.1759557129340763 1104 46-47 0.2804767923826361 1104 48-49 0.30669342956708334 1104 50-51 0.23263305559668623 1104 52-53 0.1812015534732936 1104 54-55 0.10782024770389143 1104 56-57 0.22917140106093825 1104 58-59 0.04521426762094194 1104 60-61 0.22753578656307383 1104 62-63 0.019806795522264053 1104 64-65 0.10008310684106902 1104 66-67 -0.07858465734867792 1104 68-69 0.08946465910718615 1104 70-71 0.0023591290356179684 1104 72-73 -0.023242302881875787 1104 74-75 -0.1275756428101147 1104 76-77 -0.08325360923943492 1104 78-79 0.05818973163624008 1104 80-81 0.09489388321141945 1104 82-83 0.2750600490260737 1104 84-85 0.2950733734825093 1104 86-87 0.2088534162454323 1104 88-89 0.17224021195232098 1104 90-91 0.00748593015857324 1104 92-93 -0.004192834436409498 1104 94-95 -0.05685520394584387 1104 96-97 0.08464471457443778 1104 98-99 0.1405466496573169 1104 100-101 0.20085722518371085 1105 1 0.06322851158355292 1105 2 0.13384233718017668 1105 3 0.005327850563510594 1105 4 0.16190092172691806 1105 5 0.08703190925449888 1105 6 0.08686539121956827 1105 7 0.14743845208572282 1105 8 0.10639190165352375 1105 9 0.21344068126193605 1105 10-11 0.1654555653112979 1105 12-13 0.1400612022442118 1105 14-15 0.1899386989497387 1105 16-17 0.07875757738658251 1105 18-19 0.14089568964931232 1105 20-21 0.14792727336725875 1105 22-23 0.10161694552907363 1105 24-25 0.1693663325318724 1105 26-27 -0.00397512763930763 1105 28-29 0.091660701195309 1105 30-31 0.055585441884780096 1105 32-33 0.1328239832611331 1105 34-35 0.12223220111673783 1105 36-37 0.17774187668229047 1105 38-39 0.006831295565575601 1105 40-41 -0.04481078467524213 1105 42-43 0.2127169834398117 1105 44-45 -0.02433611864970686 1105 46-47 0.11676787825425805 1105 48-49 0.07429653861314733 1105 50-51 0.19350109322647313 1105 52-53 0.14723579886994997 1105 54-55 -0.02631114104158172 1105 56-57 -0.11596426118881453 1105 58-59 -0.04335697657111126 1105 60-61 -0.09099085830585096 1105 62-63 -0.09739190808583942 1105 64-65 -0.21411984159007602 1105 66-67 -0.17524401029996994 1105 68-69 -0.04970028668527249 1105 70-71 -0.19412168321701273 1105 72-73 -0.16576653852692402 1105 74-75 -0.22942988476502535 1105 76-77 -0.1662857895870502 1105 78-79 -0.1419981304643727 1105 80-81 -0.07084064326136286 1105 82-83 -0.11634920354089928 1105 84-85 -0.10075278954163025 1105 86-87 0.029975713038872698 1105 88-89 0.2234182937512159 1105 90-91 0.09538675044194633 1105 92-93 0.04421033770837823 1105 94-95 -0.10204966273090577 1105 96-97 0.026927310799983672 1105 98-99 -0.11063286620341017 1105 100-101 0.25351597582903906 1106 1 0.16968561657006376 1106 2 0.2053206954232465 1106 3 0.23614735785360352 1106 4 0.11742676842924027 1106 5 0.15547882181905237 1106 6 0.020631788221258773 1106 7 0.1411161795869802 1106 8 0.10337873834624389 1106 9 0.24681172198591383 1106 10-11 0.1645231643788989 1106 12-13 0.2294888497600951 1106 14-15 0.2815373780484194 1106 16-17 0.19854424496737266 1106 18-19 0.19445046820408862 1106 20-21 0.15552874052755072 1106 22-23 0.16043076243111898 1106 24-25 0.12550463867017925 1106 26-27 0.24757430332189045 1106 28-29 0.2582033905321168 1106 30-31 0.18720423159180655 1106 32-33 0.11781758443120793 1106 34-35 -0.1859531592745043 1106 36-37 -0.002727408934056541 1106 38-39 0.09236536933494932 1106 40-41 0.1335903076964371 1106 42-43 0.18372685592026983 1106 44-45 0.09234483111948322 1106 46-47 0.3597868690673707 1106 48-49 0.41041222031706326 1106 50-51 0.4409032825404253 1106 52-53 0.17949618554210645 1106 54-55 0.2660599682412865 1106 56-57 0.2788549718657052 1106 58-59 0.3299479048220064 1106 60-61 0.24931377170466362 1106 62-63 0.29778954444855543 1106 64-65 0.2961525657411528 1106 66-67 0.30964046796685807 1106 68-69 0.20719845183111119 1106 70-71 0.2515659624706359 1106 72-73 0.2512417799519895 1106 74-75 0.25557285759065707 1106 76-77 0.3229230407982513 1106 78-79 0.2942500984309149 1106 80-81 0.415998334754093 1106 82-83 0.3428542563978496 1106 84-85 0.35872947655828114 1106 86-87 0.272741607275357 1106 88-89 0.3238702111148939 1106 90-91 0.2666731437871661 1106 92-93 0.1805288394680531 1106 94-95 0.13343299186939817 1106 96-97 0.0019998689607803044 1106 98-99 0.21978614289206888 1106 100-101 0.3304047733060713 1107 1 0.0333882192810222 1107 2 0.014568316471113008 1107 3 0.08838359978239652 1107 4 0.12187071024655438 1107 5 -0.06289578976685561 1107 6 -0.05923745692255977 1107 7 -0.034892350713363385 1107 8 0.02037633112134074 1107 9 -0.017686826678264822 1107 10-11 0.039264535797009614 1107 12-13 -0.0256452170498207 1107 14-15 -0.034383156603233545 1107 16-17 0.12354060989620308 1107 18-19 -0.03453424295584995 1107 20-21 -0.07324109753676566 1107 22-23 -0.14559591629859625 1107 24-25 0.016404574585223486 1107 26-27 -0.022431243527442746 1107 28-29 -0.05022512781287247 1107 30-31 -0.04373318594593201 1107 32-33 0.08223589226518868 1107 34-35 0.07212019915427703 1107 36-37 0.12347256844772403 1107 38-39 -0.03499998493639822 1107 40-41 -0.02254111493579103 1107 42-43 -0.1810102611182529 1107 44-45 -0.03844610651346869 1107 46-47 0.004283371321115226 1107 48-49 -0.08862919795307533 1107 50-51 -0.10790845308488883 1107 52-53 -0.20202634382119555 1107 54-55 -0.17275451388223928 1107 56-57 -0.062480903324797055 1107 58-59 -0.046663778027458136 1107 60-61 -0.010462415617283227 1107 62-63 0.13151492693882005 1107 64-65 -0.14812583224258447 1107 66-67 0.07660063297216624 1107 68-69 0.18351394369663154 1107 70-71 0.2626526602642869 1107 72-73 0.2829159052461563 1107 74-75 0.06692343462454176 1107 76-77 -0.11883827235101307 1107 78-79 0.04042950400253886 1107 80-81 0.1264571076617642 1107 82-83 -0.054162292600217654 1107 84-85 0.018474429194647257 1107 86-87 -0.27436299122430796 1107 88-89 -0.23990993978095076 1107 90-91 -0.11680693871549153 1107 92-93 -0.010769069423602673 1107 94-95 0.09830474117334376 1107 96-97 -0.22230933288530252 1107 98-99 -0.020180791423534572 1107 100-101 0.1072033257399525 1108 1 0.1534421019212715 1108 2 0.18042544347033385 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-1.4319684408183093 2316 74-75 -1.0318967110645332 2316 76-77 -0.9006667134621367 2316 78-79 -0.8477108246011937 2316 80-81 -1.225596371752502 2316 82-83 -1.452128993357654 2316 84-85 -1.301885600742537 2316 86-87 -0.8584700284224382 2316 88-89 -0.825194480202029 2316 90-91 -0.5484416109934145 2316 92-93 -0.4124214924156746 2316 94-95 -0.5374756828845548 2316 96-97 -0.5087161259621311 2316 98-99 -0.516997810165627 2316 100-101 -0.7013228947685874 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 5.0 13 13.0 14 46.0 15 135.0 16 358.0 17 728.0 18 1206.0 19 1871.0 20 2883.0 21 4004.0 22 5005.0 23 5129.0 24 4787.0 25 5114.0 26 5724.0 27 6820.0 28 8423.0 29 10583.0 30 13217.0 31 16636.0 32 21206.0 33 28172.0 34 39871.0 35 60876.0 36 108519.0 37 217183.0 38 320639.0 39 120597.0 40 153.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.10118676693934 19.412788629863254 13.73336728153195 22.752657321665456 2 15.371719806275355 19.815484306660053 41.58425645641542 23.22853943064917 3 19.068243458009167 24.777626277479232 30.076274954228094 26.077855310283503 4 11.95609440653276 16.42498586357933 38.924027755908824 32.69489197397908 5 12.486545737560933 38.429631360635625 35.972860759894765 13.110962141908677 6 31.482231461833464 37.82482079962135 17.46167701254477 13.23127072600042 7 27.78712410993927 32.586198872564985 21.755158663752855 17.871518353742886 8 25.209747866874345 37.52796060611762 19.43998582042038 17.822305706587663 9 25.258663455797244 16.469205458346 20.366213388810607 37.905917697046156 10-11 24.493986056086577 26.810149093526803 28.788556920813186 19.907307929573435 12-13 24.911303362798208 24.030476194248358 29.047740228516993 22.010480214436438 14-15 21.876605971068507 26.07354369677088 26.451253239172473 25.59859709298814 16-17 21.65594121415621 29.261671665496586 27.146617051340577 21.935770069006626 18-19 21.474240595383915 28.26652658720689 28.973970767489547 21.285262049919645 20-21 22.278327720583064 27.32227748605559 29.161365002381416 21.238029790979926 22-23 21.906677743923268 27.247328139961603 28.953080424645773 21.892913691469357 24-25 21.66826913079771 27.307573103555487 29.14166013963717 21.882497626009627 26-27 21.76357531366874 27.494571260804257 28.957434525890108 21.784418899636897 28-29 21.643818793079628 27.501484373104297 29.07568742940252 21.77900940441355 30-31 22.00324544936401 27.32421761842251 28.923470481712037 21.74906645050145 32-33 21.620343735982566 27.529079525459377 28.848018076983635 22.00255866157443 34-35 21.97300136745806 27.501948206907002 28.881288599004062 21.643761826630875 36-37 22.032462523628507 27.353023013101257 28.822174010771334 21.792340452498905 38-39 21.82531391628701 27.60403721941612 28.835096043877485 21.735552820419386 40-41 22.02251107284561 27.490115387319374 28.765633927218754 21.72173961261626 42-43 21.82264039219608 27.475757572756986 28.639978294945156 22.061623740101773 44-45 22.014193442340503 27.62304894628494 28.490458984674767 21.872298626699795 46-47 22.001419938350512 27.58591666724428 28.29761868219027 22.115044712214935 48-49 21.851178828387766 27.604935116520835 28.451112164395585 22.092773890695817 50-51 21.898549339634364 27.85647191991535 28.175550849366914 22.069427891083368 52-53 22.089794762467285 27.884410681025802 28.027345200479648 21.998449356027262 54-55 21.95797098220364 27.768412140836073 28.2039018811315 22.069714995828786 56-57 22.063554618611885 27.86708228414016 27.966844340496067 22.102518756751884 58-59 22.0269174366251 27.861537197136755 28.035365772752435 22.076179593485712 60-61 22.11705795070113 27.830189379855362 28.07174759122548 21.981005078218026 62-63 21.854475132760275 27.83257401948504 28.224591866743637 22.088358981011048 64-65 21.90259856639697 27.75345751027574 28.193796829992586 22.150147093334706 66-67 21.930324001413997 27.914859149839145 27.980409999772256 22.174406848974606 68-69 21.953643072651534 27.749744282371676 28.00714524068153 22.289467404295262 70-71 21.852395725133995 27.485907062361438 28.215135513014616 22.44656169948995 72-73 21.97384303244965 27.635525392042602 28.046951043813117 22.34368053169463 74-75 21.908985318391963 27.804551526235688 27.899808199401328 22.386654955971018 76-77 21.808975713509117 27.777618246504865 27.922335115352663 22.491070924633355 78-79 21.924641240119712 27.64039102784675 27.84961914640275 22.58534858563079 80-81 21.887346274263006 27.72710460108923 27.805288903127224 22.580260221520536 82-83 21.90662260771058 27.703570498455726 27.94340596063411 22.446400933199584 84-85 21.973694503333487 27.65998318650405 27.81707748169874 22.549244828463724 86-87 21.796746816278397 27.61131514610809 27.947783103921864 22.644154933691652 88-89 21.881556941607265 27.509572701536683 27.773063353609206 22.835807003246845 90-91 21.85274229307171 27.546952529104278 27.848862712557544 22.751442465266468 92-93 21.890221140050084 27.474371301006133 27.91971110096712 22.71569645797666 94-95 21.729760184889045 27.67899491337287 27.780885887060442 22.810359014677648 96-97 21.728373913138192 27.451596836527862 27.9360988134504 22.883930436883542 98-99 21.759218459594635 27.307325555028548 27.815047583777847 23.118408401598966 100-101 21.71287737535189 27.37000484204919 27.73206931754832 23.185048465050603 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 32.0 1 21.0 2 33.0 3 74.5 4 73.5 5 53.5 6 80.5 7 205.0 8 359.0 9 436.5 10 419.0 11 395.0 12 432.5 13 473.0 14 521.5 15 568.0 16 597.5 17 648.5 18 724.0 19 813.0 20 921.5 21 1113.0 22 1368.5 23 1643.0 24 1938.0 25 2455.0 26 3322.0 27 4720.5 28 6575.0 29 9200.5 30 12993.0 31 17232.5 32 21706.0 33 26238.5 34 30302.0 35 33488.5 36 36161.5 37 36906.5 38 36233.0 39 35460.5 40 33872.5 41 32531.0 42 32031.0 43 32419.0 44 32874.0 45 33438.0 46 34165.5 47 34598.5 48 35145.5 49 35179.0 50 34629.5 51 34078.0 52 32887.5 53 31822.0 54 30995.5 55 28912.5 56 26368.5 57 23871.5 58 21417.0 59 18496.0 60 15257.5 61 12483.0 62 9789.5 63 7311.0 64 5215.0 65 3618.0 66 2569.0 67 1846.5 68 1306.5 69 956.5 70 721.5 71 540.0 72 402.0 73 339.0 74 279.5 75 211.5 76 143.0 77 86.0 78 64.0 79 41.5 80 20.5 81 14.0 82 10.0 83 5.5 84 5.5 85 4.0 86 2.5 87 2.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0516881324245992 2 0.0015843105724015077 3 3.9607764310037693E-4 4 0.009901941077509425 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0025745046801524504 24-25 0.0 26-27 0.0 28-29 0.021536721843582997 30-31 0.017972023055679607 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.035894536405971664 50-51 0.01203085840917395 52-53 0.0 54-55 5.446067592630183E-4 56-57 0.0 58-59 0.0 60-61 1.4852911616264138E-4 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.9509705387547116E-5 80-81 0.01084262547987282 82-83 0.004356854074104147 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1009903.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.86907050048818 #Duplication Level Percentage of deduplicated Percentage of total 1 77.58242795906651 52.65447272752931 2 13.85271232883646 18.803414193375673 3 4.3322348197837 8.820742512257091 4 1.7501026264759272 4.75111354157537 5 0.868418436343265 2.946937604005238 6 0.4736896382197283 1.9289325271011288 7 0.30487442960390043 1.4484080909608268 8 0.1909962901677317 1.0370192546180386 9 0.13065133726337191 0.7980466337739763 >10 0.49719552580603127 5.362519656731107 >50 0.012745034091129462 0.5756848521370421 >100 0.0033661565995974606 0.3254034388606449 >500 1.4635443565216326E-4 0.05125396787849288 >1k 4.3906330695648975E-4 0.49605099919611134 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2031 0.2011084232842164 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1947 0.1927907927791085 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1016 0.10060372134749575 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.950970538754712E-5 0.0 12-13 0.0 0.0 0.0 2.970582323252827E-4 0.0 14-15 0.0 0.0 0.0 4.950970538754713E-4 0.0 16-17 0.0 0.0 0.0 0.0010397038131384896 0.0 18-19 0.0 0.0 0.0 0.0017328396885641492 0.0 20-21 0.0 0.0 0.0 0.0035646987879033925 0.0 22-23 0.0 0.0 0.0 0.008614688737433198 0.0 24-25 0.0 4.950970538754712E-5 0.0 0.013516149570800363 0.0 26-27 0.0 9.901941077509425E-5 0.0 0.015694576607852437 0.0 28-29 0.0 9.901941077509425E-5 0.0 0.017823493939516964 0.0 30-31 0.0 9.901941077509425E-5 0.0 0.021586231548970547 0.0 32-33 0.0 9.901941077509425E-5 0.0 0.028319551481676954 0.0 34-35 0.0 9.901941077509425E-5 0.0 0.039360215783099964 0.0 36-37 0.0 9.901941077509425E-5 0.0 0.05723321942800447 0.0 38-39 0.0 9.901941077509425E-5 0.0 0.08079983919247691 0.0 40-41 0.0 9.901941077509425E-5 0.0 0.11070370124655536 0.0 42-43 0.0 9.901941077509425E-5 0.0 0.13951834978210778 0.0 44-45 0.0 9.901941077509425E-5 0.0 0.17516533766114173 0.0 46-47 0.0 9.901941077509425E-5 0.0 0.22353631982477526 0.0 48-49 0.0 9.901941077509425E-5 0.0 0.2688377002543809 0.0 50-51 0.0 9.901941077509425E-5 0.0 0.32250622089448194 0.0 52-53 0.0 9.901941077509425E-5 0.0 0.37548160565915734 0.0 54-55 0.0 9.901941077509425E-5 0.0 0.42999179129084675 0.0 56-57 0.0 9.901941077509425E-5 0.0 0.49915684971725005 0.0 58-59 0.0 9.901941077509425E-5 0.0 0.5773821842295745 4.950970538754712E-5 60-61 0.0 9.901941077509425E-5 0.0 0.6602614310483284 9.901941077509425E-5 62-63 0.0 9.901941077509425E-5 0.0 0.7463588087172728 9.901941077509425E-5 64-65 0.0 9.901941077509425E-5 0.0 0.8370110792818717 9.901941077509425E-5 66-67 0.0 9.901941077509425E-5 0.0 0.9502892852085794 9.901941077509425E-5 68-69 0.0 9.901941077509425E-5 0.0 1.0850547032734825 1.980388215501885E-4 70-71 0.0 9.901941077509425E-5 0.0 1.232148037979885 1.980388215501885E-4 72-73 0.0 9.901941077509425E-5 0.0 1.3799345085617134 2.970582323252827E-4 74-75 0.0 9.901941077509425E-5 0.0 1.5297013673590434 2.970582323252827E-4 76-77 0.0 9.901941077509425E-5 0.0 1.6934299630756617 2.970582323252827E-4 78-79 0.0 9.901941077509425E-5 0.0 1.8886962411241477 2.970582323252827E-4 80-81 0.0 9.901941077509425E-5 0.0 2.1056477701323795 3.4656793771282985E-4 82-83 0.0 9.901941077509425E-5 0.0 2.331808104342694 4.950970538754713E-4 84-85 0.0 9.901941077509425E-5 0.0 2.562275782921726 4.950970538754713E-4 86-87 0.0 9.901941077509425E-5 0.0 2.8098738195648494 4.950970538754713E-4 88-89 0.0 9.901941077509425E-5 0.0 3.092821785854681 4.950970538754713E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1930 0.0 44.088734 1 GTATCAA 2815 0.0 31.409895 1 ACGATAC 65 0.005839886 29.234718 3 TCAACGC 3305 0.0 26.30461 4 ATCAACG 3335 0.0 25.925539 3 CAACGCA 3425 0.0 25.238035 5 AACGCAG 3520 0.0 24.826752 6 GTACATG 3280 0.0 24.05837 1 TATCAAC 3650 0.0 23.948439 2 TACATGG 3405 0.0 22.881208 2 ACGCAGA 3830 0.0 22.693266 7 ACATGGG 3340 0.0 22.473093 3 CTACACT 445 0.0 22.418756 4 CGCAGAG 3920 0.0 21.92993 8 GAGTACT 2330 0.0 21.81085 12-13 GAGTACA 2590 0.0 20.189468 1 AAGACGG 190 8.257648E-6 19.99775 5 TACCGTG 190 8.257648E-6 19.99775 7 GTATAGG 430 0.0 19.899218 1 GTGTAGC 715 0.0 19.280748 1 >>END_MODULE