FastQCFastQC Report
Fri 10 Feb 2017
SRR2031671_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031671_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327961
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC93590.7047646730589227No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC87490.6588295891219698No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC62880.473507881632066No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT59930.45129337382649043No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT49010.3690620432377156No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC41560.3129609981016009No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA39050.2940598406127891No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA38970.2934574132824684No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA35810.26966153373480095No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT32080.24157335945859856No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG31490.23713045789748344No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC31330.23592560323684206No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC30730.23140739825943685No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC30300.2281693513589631No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA29360.22109083022769493No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC28410.21393700568013668No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA28410.21393700568013668No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA28240.2126568476032052No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA27520.20723500163031894No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT26960.20301801031807412No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC26560.2000058736664706No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC26240.19759616434518787No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC26220.1974455575126077No Hit
ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC25360.1909694637116602No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC25270.19029173296504942No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA25190.18968930563472872No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG22500.1694326866526954No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG22230.16739949441286303No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT22140.16672176366625224No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC21720.16355902018206858No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT21410.1612246142770759No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA21400.16114931086078582No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA20950.1577606571277319No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT20930.15761005029515174No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT20920.15753474687886165No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT20790.15655580246709053No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG20730.15610398196935002No Hit
ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC20560.15482382389241853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20550.15474852047612844No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC20430.1538448794806474No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG20090.15128456332678444No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT19630.14782060617744044No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT19150.14420604219551628No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC19150.14420604219551628No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG18960.14277527728600464No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC18730.14104329871133262No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT18710.14089269187875245No Hit
ATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGC18170.13682630739908777No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT17630.1327599229194231No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA17570.13230810242168256No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA17150.12914535893749893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17050.12839232477459805No Hit
ATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGCTTCA16810.12658504278363597No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT16700.125756705204445No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA16700.125756705204445No Hit
CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT16690.12568140178815493No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG16690.12568140178815493No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA16630.1252295812904144No Hit
TTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGT16370.12327169246687214No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT16060.12093728656187945No Hit
ACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGGTAAAA15880.11958182506865789No Hit
GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG15840.11928061140349755No Hit
ATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATG15580.11732272257995528No Hit
ATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTT15350.11559074400528328No Hit
GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC15250.11483770984238241No Hit
GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG15160.11415997909577164No Hit
ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT15070.11348224834916085No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC15040.1132563381002906No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA14820.11159966294190869No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA14700.11069602194642764No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC14640.11024420144868713No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG14560.10964177411836643No Hit
TTCTAGGAGTCTGCCTAATAGTCCAAATCATTACAGGTCTTTTCTTAGCC14520.10934056045320609No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG14420.10858752629030523No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC14270.10745797504595392No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC14200.1069308511319233No Hit
GGCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTG14100.10617781696902243No Hit
AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA14060.10587660330386209No Hit
TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT14060.10587660330386209No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT13830.10414462472919009No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA13670.10293977006854871No Hit
TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA13600.1024126461545181No Hit
CTATAAATGTATATGATCCATAATATAAGCCTCGTCCGACATGAAGGAAT13550.10203612907306765No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT13470.10143370174274695No Hit
TATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGCT13470.10143370174274695No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC13460.10135839832645686No Hit
CACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCG13310.10022884708210558No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAT301.2819628E-463.5252461
ATGTATT352.7467436E-454.450211
GTATGGT1800.047.643941
AAAGACA1200.047.5306174
GACATAT3400.043.440061
GTAAGGT1006.330083E-1042.7775574
TAACTTT16800.040.1595276
TACCTGG1101.5897967E-938.9371952
CTTAATA25050.038.3758932
GTCTTCT1900.037.511438
ACATATA3850.037.0830462
ACTTAAT25950.036.7197951
AGGGTCT1950.036.5620165
ACTTCAG26750.035.3472188
ATTGAAC3400.034.937118
GTTGTGC1502.5465852E-1134.843958
GTGACCA550.00257591634.555989
ACCTGGG1255.6243152E-934.218173
ATCTATA18900.034.031381
GATGGCC702.0498295E-433.9389089
Produced by FastQC (version 0.11.5)