##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031671_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1327961 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5575141137428 34.0 31.0 34.0 31.0 34.0 2 32.7763902704974 34.0 31.0 34.0 31.0 34.0 3 32.868783797114524 34.0 31.0 34.0 31.0 34.0 4 36.31221925945114 37.0 37.0 37.0 35.0 37.0 5 36.07467689186655 37.0 37.0 37.0 35.0 37.0 6 36.13688579709796 37.0 37.0 37.0 35.0 37.0 7 36.06597332301175 37.0 37.0 37.0 35.0 37.0 8 36.164758603603566 37.0 37.0 37.0 35.0 37.0 9 38.03792355347785 39.0 39.0 39.0 35.0 39.0 10-11 38.005959135848116 39.0 38.5 39.0 35.0 39.0 12-13 38.05582468159833 39.0 39.0 39.0 35.5 39.0 14-15 39.5172369519888 41.0 39.5 41.0 37.0 41.0 16-17 39.47846246990687 41.0 39.0 41.0 37.0 41.0 18-19 39.529578428884584 41.0 39.5 41.0 37.0 41.0 20-21 39.56825765214491 41.0 40.0 41.0 37.0 41.0 22-23 39.48399124673089 41.0 39.5 41.0 36.5 41.0 24-25 39.43374617176258 41.0 39.0 41.0 36.0 41.0 26-27 39.34273935755643 41.0 39.0 41.0 36.0 41.0 28-29 39.27610788268632 41.0 39.0 41.0 36.0 41.0 30-31 39.1751504750516 41.0 39.0 41.0 36.0 41.0 32-33 39.0528110388784 40.0 39.0 41.0 35.5 41.0 34-35 39.00384235681621 40.0 39.0 41.0 35.0 41.0 36-37 38.89040039579476 40.0 38.0 41.0 35.0 41.0 38-39 38.773153353148174 40.0 38.0 41.0 35.0 41.0 40-41 38.726916679028974 40.0 38.0 41.0 35.0 41.0 42-43 38.64921635499837 40.0 38.0 41.0 35.0 41.0 44-45 38.60731979327706 40.0 38.0 41.0 35.0 41.0 46-47 38.62110860183394 40.0 38.5 41.0 35.0 41.0 48-49 38.74936538045921 41.0 39.0 41.0 35.0 41.0 50-51 38.71715773279486 41.0 39.0 41.0 35.0 41.0 52-53 38.69845951801295 41.0 39.0 41.0 35.0 41.0 54-55 38.641410779382824 41.0 39.0 41.0 35.0 41.0 56-57 38.57413621333759 40.0 39.0 41.0 35.0 41.0 58-59 38.46366045388381 40.0 38.5 41.0 34.0 41.0 60-61 38.27695354005125 40.0 38.0 41.0 34.0 41.0 62-63 38.108232094165416 40.0 38.0 41.0 34.0 41.0 64-65 37.87274889849928 40.0 37.5 41.0 34.0 41.0 66-67 37.65118591585144 39.5 37.0 41.0 33.0 41.0 68-69 37.36369629830996 39.0 36.5 41.0 33.0 41.0 70-71 36.997849334430754 39.0 36.0 40.5 32.5 41.0 72-73 36.64147064559878 38.0 36.0 40.0 32.0 41.0 74-75 36.228120027621294 37.0 35.0 39.0 32.0 41.0 76-77 35.54081859331713 36.5 35.0 39.0 31.0 40.0 78-79 35.41678746589697 36.0 35.0 39.0 31.5 40.0 80-81 35.06106542285504 36.0 35.0 37.0 31.0 39.0 82-83 34.708438350222636 35.5 35.0 37.0 31.0 39.0 84-85 34.32874082898519 35.0 35.0 36.5 31.0 38.0 86-87 34.05614509763464 35.0 34.0 36.0 31.0 37.0 88-89 33.78638002170244 35.0 34.0 36.0 31.0 37.0 90-91 33.61040685682788 35.0 34.0 36.0 30.0 36.5 92-93 33.4715970574437 35.0 34.0 35.0 30.0 36.0 94-95 33.33103645363079 35.0 34.0 35.0 30.0 36.0 96-97 33.22946268753375 35.0 34.0 35.0 30.0 36.0 98-99 33.17361315580804 35.0 34.0 35.0 30.0 36.0 100-101 32.79103038417544 35.0 33.5 35.0 28.5 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09166164035514868 1101 2 0.1368834475037204 1101 3 0.12584410248398115 1101 4 0.10464318876248058 1101 5 0.025445679567802415 1101 6 0.08921798673672043 1101 7 0.16199939393588636 1101 8 0.13117331150720446 1101 9 0.15665556976276918 1101 10-11 -0.026476942047878538 1101 12-13 0.0037565060025528396 1101 14-15 0.0961945657384149 1101 16-17 0.19651621200554814 1101 18-19 0.12628626635851248 1101 20-21 -0.09531436332876808 1101 22-23 0.016892598194871766 1101 24-25 0.017914107868726603 1101 26-27 0.0756209577235154 1101 28-29 -0.0216508952982295 1101 30-31 -0.06348033015201793 1101 32-33 -0.12218201251265981 1101 34-35 -0.16661612676798399 1101 36-37 -0.13713034451327388 1101 38-39 -0.17131095104922878 1101 40-41 -0.19902138796906144 1101 42-43 -0.13563449972414787 1101 44-45 -0.1609602785154607 1101 46-47 -0.03692641660466478 1101 48-49 -0.08802370590548492 1101 50-51 0.14054814245025682 1101 52-53 -0.11477435303770278 1101 54-55 0.033928275346873704 1101 56-57 0.1938973544374889 1101 58-59 0.016595527595640647 1101 60-61 -0.17445812861667065 1101 62-63 -0.17492742329760347 1101 64-65 -0.12028562758565187 1101 66-67 -0.04509181602212209 1101 68-69 0.054675523731205544 1101 70-71 -0.08979890212906838 1101 72-73 -0.191105514836714 1101 74-75 0.007418721324036426 1101 76-77 -0.026609548674954908 1101 78-79 -0.005511972878259996 1101 80-81 0.09776786833051432 1101 82-83 0.09347033257226656 1101 84-85 0.0782549041832965 1101 86-87 -0.1007749659868793 1101 88-89 -0.05421216914317739 1101 90-91 0.020231364566498655 1101 92-93 -0.17245499083539073 1101 94-95 -0.1111720356865078 1101 96-97 -0.17102376022570098 1101 98-99 -0.3338314699964329 1101 100-101 -0.14101944921480225 1102 1 0.14931656495458157 1102 2 0.2715588716319033 1102 3 0.21259893283271225 1102 4 0.17943216420185593 1102 5 0.025396301899675677 1102 6 0.042718950034384306 1102 7 0.17290666094259421 1102 8 0.06649289763571176 1102 9 0.2257245321733734 1102 10-11 0.24215446492112846 1102 12-13 0.08779123322992177 1102 14-15 0.18230358816453673 1102 16-17 0.21514242132528238 1102 18-19 0.20853863302148312 1102 20-21 0.1638334993978532 1102 22-23 0.184946026564333 1102 24-25 0.3042777284819138 1102 26-27 0.18561231230022202 1102 28-29 -0.20413386433410352 1102 30-31 -0.12177803071197957 1102 32-33 0.17241165259240177 1102 34-35 0.21611364398103206 1102 36-37 0.2440513930262327 1102 38-39 0.1510780098367306 1102 40-41 0.07883844529034434 1102 42-43 0.12179900501611485 1102 44-45 0.1898234403679524 1102 46-47 0.2390904168487893 1102 48-49 0.11465646194393742 1102 50-51 0.1857897811651199 1102 52-53 0.14637287211059657 1102 54-55 -0.05890780465534817 1102 56-57 -0.1209467853862094 1102 58-59 0.09852087549163713 1102 60-61 0.3029624166513898 1102 62-63 0.18492359474863207 1102 64-65 0.1637303881043195 1102 66-67 0.1449792878199787 1102 68-69 -0.005486833506694211 1102 70-71 0.08781776777169625 1102 72-73 0.14684564112048548 1102 74-75 0.07712136380892787 1102 76-77 0.06587742255085516 1102 78-79 -0.11799733283280034 1102 80-81 -0.07289261428855554 1102 82-83 -0.27293448807418486 1102 84-85 -0.05329456704251356 1102 86-87 -0.09452002822006733 1102 88-89 0.03548539697091968 1102 90-91 0.07993156415622593 1102 92-93 0.20194554694806044 1102 94-95 0.24136978806467368 1102 96-97 0.2286460157902681 1102 98-99 0.15794238374864733 1102 100-101 0.02814107845055247 1103 1 0.07540255786238248 1103 2 0.260809758156725 1103 3 0.22911045765540905 1103 4 0.12161249378049632 1103 5 0.08699022138236501 1103 6 0.10397390414369312 1103 7 0.2061317860990428 1103 8 0.14644309580008752 1103 9 0.27587185173949536 1103 10-11 0.17248730284353542 1103 12-13 0.11157747645061278 1103 14-15 0.21525167431389747 1103 16-17 0.2736693560353345 1103 18-19 0.2112405781612381 1103 20-21 0.10871251483381172 1103 22-23 0.02143760457852295 1103 24-25 0.14083775142726296 1103 26-27 0.14246919068987296 1103 28-29 0.21686438869050306 1103 30-31 0.10009436186206955 1103 32-33 0.16817784470754304 1103 34-35 0.15161244456509593 1103 36-37 0.19372807220853616 1103 38-39 0.29297738926936034 1103 40-41 0.20662513177344977 1103 42-43 0.14937905299274945 1103 44-45 0.26712384191154115 1103 46-47 0.20427476186339533 1103 48-49 -0.09565525582827661 1103 50-51 0.05522618604621954 1103 52-53 0.11849948871468996 1103 54-55 0.0978441248398596 1103 56-57 0.07889937648279499 1103 58-59 0.15033042910030758 1103 60-61 0.11312225186207314 1103 62-63 -0.049904445510023265 1103 64-65 0.2003451669946017 1103 66-67 0.11121312177908749 1103 68-69 0.039240299367733655 1103 70-71 0.16782011445504708 1103 72-73 0.02296683427859847 1103 74-75 -0.018092054005009572 1103 76-77 -0.0074694693974066695 1103 78-79 0.23297197776377487 1103 80-81 0.2570315096163327 1103 82-83 0.03566741013190722 1103 84-85 -0.03618659353396936 1103 86-87 -0.0876918315158477 1103 88-89 0.020127658130704162 1103 90-91 0.08358196048497746 1103 92-93 -0.05319108217167212 1103 94-95 0.1049557809724746 1103 96-97 0.046530350375164176 1103 98-99 0.025041883122867148 1103 100-101 0.1935985513333165 1104 1 0.027530217436847693 1104 2 0.12339841063899826 1104 3 0.0810608122653349 1104 4 0.0462578838514176 1104 5 -0.08144949492969289 1104 6 -0.011274322806663406 1104 7 0.0873374598579062 1104 8 0.018783521332004227 1104 9 0.20494986592389353 1104 10-11 0.03906531702793359 1104 12-13 0.09916612893288601 1104 14-15 0.19796444027134186 1104 16-17 0.22801332766653815 1104 18-19 0.13012355688464083 1104 20-21 0.05374797582672386 1104 22-23 0.16948724996859 1104 24-25 0.0606072549733625 1104 26-27 0.09016422615086128 1104 28-29 0.04975445961249392 1104 30-31 0.10585677320958098 1104 32-33 0.07863883761534396 1104 34-35 0.2003713098133204 1104 36-37 0.27972098000996226 1104 38-39 0.14182490699985095 1104 40-41 -0.012789761843571057 1104 42-43 0.18218047143246707 1104 44-45 0.31765575680515923 1104 46-47 0.2636719249839601 1104 48-49 0.3365255952355497 1104 50-51 0.34423327824480765 1104 52-53 0.35121225467213435 1104 54-55 0.25786185533631567 1104 56-57 0.24999866726293618 1104 58-59 0.12550773406485405 1104 60-61 0.31702296108192485 1104 62-63 0.25816293037650695 1104 64-65 0.1812848833066596 1104 66-67 0.18169184518335157 1104 68-69 0.22496724972234006 1104 70-71 0.26445132012880634 1104 72-73 0.2042611605197422 1104 74-75 0.009390215498541465 1104 76-77 0.1760411689004613 1104 78-79 0.31408899904037213 1104 80-81 0.29736838904895535 1104 82-83 0.36456811935176603 1104 84-85 0.42923893838093363 1104 86-87 0.28863795571819395 1104 88-89 0.2089574453647458 1104 90-91 0.2280855065842644 1104 92-93 0.17996494619711711 1104 94-95 0.2534486887738936 1104 96-97 0.13872893193544655 1104 98-99 0.13940358525052687 1104 100-101 0.20733968608509912 1105 1 0.02884441252577119 1105 2 -0.011758758183844975 1105 3 -0.08103067353244597 1105 4 0.017301042909117825 1105 5 -0.11780592245739996 1105 6 -0.09626487842162135 1105 7 0.08644648013660827 1105 8 0.014555822554427778 1105 9 0.11157073623288483 1105 10-11 0.1379885680347428 1105 12-13 0.13930031047890878 1105 14-15 -0.0057714377000834816 1105 16-17 -0.10139969489306822 1105 18-19 0.033205010250760836 1105 20-21 0.038106826819273465 1105 22-23 0.01337201065143745 1105 24-25 0.046652285088477186 1105 26-27 0.03396790767907021 1105 28-29 -8.064121220670017E-4 1105 30-31 -0.009721116237649596 1105 32-33 0.070878404242805 1105 34-35 0.11168987070287528 1105 36-37 0.14402922335434454 1105 38-39 0.028917729267213588 1105 40-41 -0.027983193541068374 1105 42-43 -0.08283476432076498 1105 44-45 -0.09584124950297479 1105 46-47 0.013968175741290167 1105 48-49 0.0853316824090129 1105 50-51 0.23846507552909912 1105 52-53 0.11168349384673348 1105 54-55 0.07447818164872189 1105 56-57 -0.036101603594900666 1105 58-59 0.048620639015133804 1105 60-61 0.09011047311015119 1105 62-63 0.15799899010777807 1105 64-65 0.08334839234119329 1105 66-67 0.06735464499802646 1105 68-69 0.04181077586369497 1105 70-71 0.09331861760912119 1105 72-73 0.20582928482922824 1105 74-75 0.16546981562683527 1105 76-77 0.07073627563983109 1105 78-79 -0.012664449051150939 1105 80-81 0.12916700981234897 1105 82-83 0.11686684703272476 1105 84-85 0.04288562928224593 1105 86-87 0.07552674362937495 1105 88-89 0.12540804944899975 1105 90-91 0.14214828501543053 1105 92-93 0.13900215351028322 1105 94-95 0.11775693211826876 1105 96-97 0.08601188927534054 1105 98-99 0.08575546878165596 1105 100-101 0.09647062446493493 1106 1 0.11073444521348108 1106 2 0.1481264282662096 1106 3 0.10646017878740821 1106 4 0.10433817029360881 1106 5 0.1413092326059413 1106 6 0.059509950415119306 1106 7 0.12498464019255096 1106 8 0.19649301661534224 1106 9 0.15104399248176748 1106 10-11 0.1859142807406613 1106 12-13 0.18809403639001943 1106 14-15 0.1840124331102828 1106 16-17 0.16624922933974773 1106 18-19 0.13461212673935563 1106 20-21 0.14251234957870196 1106 22-23 0.13743894727630845 1106 24-25 0.08459076389025455 1106 26-27 0.19785977803408628 1106 28-29 0.13399583536131132 1106 30-31 0.006746740847127342 1106 32-33 0.06964448383169497 1106 34-35 -0.09371823780080746 1106 36-37 0.08423795308714688 1106 38-39 0.08941235449692186 1106 40-41 0.11231782612034635 1106 42-43 0.23357739355598994 1106 44-45 0.2132222719879664 1106 46-47 0.08588926988515055 1106 48-49 0.2994809074988538 1106 50-51 0.34778798457269744 1106 52-53 0.25508456066951624 1106 54-55 0.2516260569475506 1106 56-57 0.34768423498310597 1106 58-59 0.30574769825958015 1106 60-61 0.30905714823334307 1106 62-63 0.24626800508558233 1106 64-65 0.3572299359817279 1106 66-67 0.40596110773240923 1106 68-69 0.19849630707963684 1106 70-71 0.21002472094131264 1106 72-73 0.35965260856766434 1106 74-75 0.343235667540128 1106 76-77 0.3456342281857303 1106 78-79 0.3472174848904075 1106 80-81 0.34245635296825583 1106 82-83 0.29428305428256607 1106 84-85 0.2937942988436433 1106 86-87 0.30212087935655774 1106 88-89 0.3638453817415339 1106 90-91 0.39585817752002583 1106 92-93 0.362675130043435 1106 94-95 0.1756841084530265 1106 96-97 0.18760399218471235 1106 98-99 0.3251563567164766 1106 100-101 0.3639447263660287 1107 1 0.09297487185386899 1107 2 0.08399691683412414 1107 3 0.10163789000955603 1107 4 0.049809607407524936 1107 5 0.04398638563340995 1107 6 0.049708180103891664 1107 7 0.052222359267922513 1107 8 0.005069453357130271 1107 9 0.02552525638851222 1107 10-11 0.007467139852323612 1107 12-13 -0.06566351207025889 1107 14-15 0.031205676259894233 1107 16-17 0.11350047340813063 1107 18-19 0.01422396970571782 1107 20-21 0.011701671250257561 1107 22-23 0.11516633782523655 1107 24-25 0.1772886150332056 1107 26-27 0.12809602912160045 1107 28-29 0.1095414546237734 1107 30-31 0.12859005367482723 1107 32-33 0.21884896016808852 1107 34-35 0.20115484027715524 1107 36-37 0.21450551440515397 1107 38-39 0.2789589079334789 1107 40-41 0.19732189388390253 1107 42-43 0.13045445470589812 1107 44-45 0.08260118737079125 1107 46-47 0.23654152358014358 1107 48-49 0.15057894701647712 1107 50-51 0.3012695153863234 1107 52-53 0.2988049988074266 1107 54-55 0.3158582248730397 1107 56-57 0.20631886512458664 1107 58-59 0.20183815402874217 1107 60-61 0.32338927791928995 1107 62-63 0.1399338428010637 1107 64-65 0.16584486890854322 1107 66-67 0.3613895831038292 1107 68-69 0.3451026117281799 1107 70-71 0.2823157444008828 1107 72-73 0.35482133734664245 1107 74-75 0.3023726087359222 1107 76-77 0.17943400696098877 1107 78-79 0.20183920854425708 1107 80-81 0.285031914563433 1107 82-83 0.24789835933916748 1107 84-85 0.3904508410136458 1107 86-87 0.25074130440577136 1107 88-89 0.07411603558639257 1107 90-91 0.24705791112641862 1107 92-93 0.3885359047406638 1107 94-95 0.3925023217680774 1107 96-97 0.29075387836432043 1107 98-99 0.253635577759006 1107 100-101 0.3169601325392719 1108 1 0.08365322139629683 1108 2 0.09834184709202276 1108 3 0.11319325798608304 1108 4 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-1.2379500814501725 2316 82-83 -1.6254294909296902 2316 84-85 -1.4825001995382365 2316 86-87 -1.1031713346164125 2316 88-89 -0.9254654882375277 2316 90-91 -0.7632651805610777 2316 92-93 -0.7875961435145342 2316 94-95 -0.7966174335229965 2316 96-97 -0.7720857089384552 2316 98-99 -0.7448988498321611 2316 100-101 -1.0426800594706371 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 3.0 13 16.0 14 72.0 15 229.0 16 546.0 17 1039.0 18 1744.0 19 2332.0 20 3160.0 21 4614.0 22 5507.0 23 4309.0 24 3735.0 25 3804.0 26 4804.0 27 6254.0 28 8195.0 29 10459.0 30 13200.0 31 16813.0 32 21330.0 33 28176.0 34 39090.0 35 58051.0 36 99290.0 37 235270.0 38 544052.0 39 211795.0 40 71.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.7059681083097 28.78471790583174 16.37174575549704 21.137568230361524 2 12.096320544361534 24.725420783001653 43.61406116687413 19.56419750576268 3 12.747118690015851 33.70852174960748 34.549514102511004 18.994845457865665 4 8.81816230545901 22.20582088484378 48.022714178663286 20.953302631033925 5 8.112888857428795 43.94142599067292 39.466746387883376 8.478938764014906 6 21.331123429076605 48.11609678296275 21.341364693692057 9.211415094268581 7 21.71675222389814 35.153291399370914 30.330107585990852 12.79984879074009 8 21.24399737642898 39.74228158808881 24.48279731106561 14.530923724416606 9 24.270140463462404 17.194857379094717 27.047029242575647 31.48797291486723 10-11 19.419772116801624 29.7524550796296 35.06522405401966 15.762548749549122 12-13 19.913386010583142 29.450224818349334 32.646515974490214 17.989873196577307 14-15 19.843617395390375 31.055957215611002 29.154395347453725 19.946030041544898 16-17 17.72141651750315 34.12152164107229 29.914508031485866 18.242553809938695 18-19 17.17226635420769 33.190959674267546 32.78040544865399 16.856368522870778 20-21 18.144697020469728 32.9827833799336 32.02025511291371 16.852264486682966 22-23 19.23147304451289 31.861807474791593 30.93943204837604 17.967287432319477 24-25 18.946753707375443 32.178806455912486 30.691526332475128 18.182913504236947 26-27 18.51342019833414 31.98433538334334 31.508944916304017 17.99329950201851 28-29 18.637474772619342 29.99537913848613 33.17503650179317 18.192109587101356 30-31 19.136401517333994 31.77634499954245 30.930310414781392 18.156943068342166 32-33 17.72315516914848 32.51926544501889 31.50545516978856 18.252124216044077 34-35 17.87774640972137 31.988665329780012 31.987460475119374 18.14612778537924 36-37 19.03297611902759 30.72258899169479 30.81901501625424 19.42541987302338 38-39 18.058248698568708 32.78970542056581 31.978198154915695 17.173847725949784 40-41 17.320124612093277 31.3472684815292 33.47816690399794 17.854440002379587 42-43 18.041380733319727 31.382359873520382 30.80564865986275 19.770610733297136 44-45 18.736318310552793 31.536844832039495 31.35253972066951 18.374297136738203 46-47 18.223163180243997 30.978658258789228 31.917955421883626 18.880223139083153 48-49 17.32361077892802 31.512526388938582 31.542959591261 19.62090324087239 50-51 17.897900363081515 30.88158541829687 30.18132988301711 21.039184335604507 52-53 18.256371986827926 30.5489016620217 32.05060238967861 19.14412396147176 54-55 18.983901143980034 30.685495667575207 30.960505711240206 19.370097477204553 56-57 19.21720592698129 31.069210616878056 29.995233293748836 19.71835016239182 58-59 18.080312599541703 30.670328420789467 30.616561781558342 20.632797198110488 60-61 19.518238682551175 32.86656493171296 29.305050865594268 18.310145520141603 62-63 18.321321183378124 32.32131817124147 31.617457139178036 17.739903506202364 64-65 18.28208810349099 31.21902676358718 30.8778269843768 19.621058148545025 66-67 18.909252606062978 31.469034105670275 30.172949356193442 19.448763932073305 68-69 18.489059543164295 31.69833300827359 30.453680492122885 19.35892695643923 70-71 18.49180811785888 30.706097543527257 32.12413617568588 18.677958162927975 72-73 18.911285798302814 31.29715405798815 30.46550312848043 19.32605701522861 74-75 17.990626230740208 31.450358858430334 30.06737396655474 20.49164094427472 76-77 17.29011620070168 31.91012386658946 31.83354029222244 18.96621964048643 78-79 17.637548434270776 31.822657242182462 30.485681227477198 20.05411309606957 80-81 18.1974318421449 32.07689411055882 29.96339810212381 19.762275945172465 82-83 18.649182313019434 31.221138466080074 30.78251092709347 19.347168293807027 84-85 17.773187616202584 30.854068756537277 31.175840254344816 20.19690337291532 86-87 16.89917851503169 32.25956936988361 31.88493487384042 18.95631724124428 88-89 18.708983170439495 29.928439163499533 31.021317644117563 20.341260021943416 90-91 17.82974048183644 30.75173141379905 32.267438576885915 19.151089527478593 92-93 19.04235139435571 31.661020165501846 30.376005018219658 18.920623421922784 94-95 18.193832499599008 29.710812290421178 31.49618098724285 20.599174222736963 96-97 18.991408633235466 29.89342307492464 31.25042828818015 19.864740003659744 98-99 17.794310224471953 30.82503928955745 31.167330968304043 20.21331951766656 100-101 17.628981574007067 30.465540780188576 31.96837105908984 19.93710658671452 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 68.0 1 48.0 2 45.0 3 82.0 4 70.5 5 48.5 6 91.0 7 186.0 8 285.0 9 337.5 10 298.0 11 246.0 12 257.5 13 282.5 14 319.0 15 362.5 16 377.5 17 402.5 18 474.0 19 542.5 20 643.5 21 820.5 22 1020.5 23 1244.0 24 1444.0 25 1833.0 26 2720.0 27 4082.0 28 5174.5 29 8788.5 30 17406.0 31 27021.0 32 50317.0 33 78221.5 34 96460.5 35 113259.5 36 138565.0 37 151018.0 38 128445.5 39 106136.5 40 84085.0 41 60370.5 42 47202.0 43 40793.0 44 37646.0 45 32174.5 46 23869.0 47 16039.5 48 13536.5 49 10837.5 50 7939.0 51 4911.0 52 2157.0 53 1208.0 54 802.0 55 720.0 56 651.5 57 597.5 58 547.0 59 492.0 60 469.0 61 470.0 62 373.0 63 213.5 64 104.5 65 81.0 66 61.0 67 34.0 68 24.0 69 15.5 70 10.5 71 11.5 72 16.5 73 27.0 74 22.0 75 8.0 76 6.5 77 7.5 78 7.0 79 4.0 80 1.5 81 0.5 82 1.5 83 2.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04939904108629697 2 0.0015060683258017367 3 4.51820497740521E-4 4 0.011747332941253547 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.002334405904992692 24-25 0.0 26-27 0.0 28-29 0.02157442876710988 30-31 0.017884561368895623 32-33 3.7651708145043414E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.034903133450455245 50-51 0.012048546606413893 52-53 0.0 54-55 7.153824547558249E-4 56-57 0.0 58-59 0.0 60-61 3.7651708145043414E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.1295512443513026E-4 80-81 0.01212385002270398 82-83 0.005195935724015992 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1327961.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.119201994165612 #Duplication Level Percentage of deduplicated Percentage of total 1 72.65582754646664 7.352189949959581 2 9.592910865634908 1.9414520552277146 3 3.5785149968319074 1.0863514827627894 4 1.9259018220079935 0.779543582313219 5 1.259639336131727 0.637327244105681 6 0.9485228581726463 0.5758976638759384 7 0.6850992440677152 0.4852860345739964 8 0.5941408655453726 0.48097851451536144 9 0.4980735857036084 0.45360964995238534 >10 5.18379821663951 11.968906935063425 >50 1.260131297465409 9.106257363006561 >100 1.5219085729507829 32.07865760997843 >500 0.18751953831330492 12.974371185447595 >1k 0.10501094145545076 17.915890009202567 >5k 0.0030003126130128785 2.1632807200147535 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 8457 0.6368409915652643 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 8132 0.6123673812709861 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 6186 0.4658269331704772 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 5726 0.4311873616770372 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 4713 0.35490500097517924 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 4139 0.3116808400246694 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 3951 0.29752379776213306 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 3776 0.2843456999113679 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 3757 0.2829149350018562 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 3367 0.25354660264872236 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 3044 0.2292235991870243 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2987 0.22493130445848936 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 2959 0.22282280880236696 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 2882 0.21702444574803023 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 2865 0.2157442876710988 No Hit CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC 2831 0.21318397151723584 No Hit CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA 2799 0.21077426219595305 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 2774 0.20889167678870088 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 2749 0.20700909138144868 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 2716 0.20452407864387587 No Hit ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA 2588 0.19488524135874474 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 2582 0.1944334208610042 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 2533 0.19074355346278996 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 2454 0.18479458357587308 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 2403 0.18095410934507866 No Hit ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC 2402 0.18087880592878858 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 2361 0.177791365860895 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 2267 0.17071284472962683 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 2260 0.17018572081559624 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 2228 0.16777601149431345 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 2225 0.1675501012454432 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 2208 0.16626994316851174 No Hit ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC 2202 0.1658181226707712 No Hit CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT 2161 0.16273068260287765 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 2139 0.16107400744449574 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2060 0.15512503755757887 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 2055 0.15474852047612844 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 2025 0.15248941798742585 No Hit CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG 1989 0.14977849500098273 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1982 0.1492513710869521 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 1960 0.1475946959285702 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 1945 0.1464651446842189 No Hit GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC 1924 0.14488377294212706 No Hit ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG 1921 0.1446578626932568 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 1913 0.1440554353629361 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 1907 0.1436036148651956 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1886 0.14202224312310377 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 1806 0.1359979698198968 No Hit GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA 1794 0.13509432882441577 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1792 0.1349437219918356 No Hit ATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGC 1741 0.13110324776104118 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 1727 0.13004899993297997 No Hit ATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGCTTCA 1714 0.12907005552120882 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 1703 0.12824171794201786 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 1676 0.12620852570218552 No Hit TTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGT 1664 0.12530488470670448 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1656 0.12470245737638379 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 1575 0.11860288065688676 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 1567 0.11800045332656607 No Hit GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG 1564 0.11777454307769582 No Hit GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC 1526 0.1149130132586725 No Hit CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG 1520 0.11446119276093199 No Hit TTCTAGGAGTCTGCCTAATAGTCCAAATCATTACAGGTCTTTTCTTAGCC 1516 0.11415997909577164 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 1496 0.11265391076996989 No Hit GGCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTG 1483 0.11167496635819878 No Hit GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG 1474 0.11099723561158799 No Hit ACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGGTAAAA 1473 0.1109219321952979 No Hit TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA 1467 0.11047011169755738 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 1461 0.11001829119981688 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1458 0.1097923809509466 No Hit ATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATG 1458 0.1097923809509466 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1439 0.10836161604143495 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1410 0.10617781696902243 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 1408 0.10602721013644226 No Hit CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT 1400 0.10542478280612157 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1397 0.10519887255725131 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1388 0.10452114181064052 No Hit AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA 1387 0.10444583839435044 No Hit ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT 1377 0.10369280423144958 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 1359 0.10233734273822802 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1357 0.10218673590564782 No Hit CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC 1339 0.10083127441242627 No Hit ACCTACTACAATGCTAAAAATTATTCTTCCCTCACTAATGCTACTACCAC 1338 0.10075597099613617 No Hit TAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCA 1334 0.10045475733097584 No Hit AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT 1328 0.1000029368332353 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.530341629008683E-5 0.0 3 0.0 0.0 0.0 7.530341629008683E-5 0.0 4 0.0 0.0 0.0 7.530341629008683E-5 0.0 5 0.0 0.0 0.0 7.530341629008683E-5 0.0 6 0.0 0.0 0.0 7.530341629008683E-5 0.0 7 0.0 0.0 0.0 7.530341629008683E-5 0.0 8 0.0 0.0 0.0 7.530341629008683E-5 0.0 9 0.0 0.0 0.0 2.259102488702605E-4 0.0 10-11 0.0 0.0 0.0 3.3886537330539077E-4 0.0 12-13 0.0 0.0 0.0 8.283375791909551E-4 0.0 14-15 0.0 0.0 0.0 0.002146147364267475 0.0 16-17 0.0 0.0 0.0 0.005798363054336686 0.0 18-19 0.0 0.0 0.0 0.009337623619970767 0.0 20-21 0.0 0.0 0.0 0.014646514468421887 0.0 22-23 0.0 0.0 0.0 0.027071578156286216 0.0 24-25 0.0 0.0 0.0 0.042019306289868455 0.0 26-27 0.0 0.0 0.0 0.049549647918877136 0.0 28-29 0.0 0.0 0.0 0.055724528054664255 0.0 30-31 0.0 0.0 0.0 0.06423381409544407 0.0 32-33 0.0 0.0 0.0 0.07929449735346143 0.0 34-35 0.0 0.0 0.0 0.10316568031741896 0.0 36-37 0.0 0.0 0.0 0.1334753053741789 0.0 38-39 0.0 0.0 0.0 0.1823095708383002 0.0 40-41 0.0 0.0 0.0 0.24304177607625524 0.0 42-43 0.0 0.0 0.0 0.2996322934182555 0.0 44-45 0.0 0.0 0.0 0.36785718857707417 0.0 46-47 0.0 0.0 0.0 0.4527994421522921 0.0 48-49 0.0 0.0 0.0 0.5450084753995035 0.0 50-51 0.0 0.0 0.0 0.6428276131603262 0.0 52-53 0.0 0.0 0.0 0.7434706290320273 0.0 54-55 0.0 0.0 0.0 0.844640768817759 0.0 56-57 0.0 0.0 0.0 0.9596667372008666 0.0 58-59 0.0 0.0 0.0 1.094572807484557 0.0 60-61 0.0 3.7651708145043414E-5 0.0 1.2433723580737688 0.0 62-63 0.0 7.530341629008683E-5 0.0 1.3931885047828965 0.0 64-65 0.0 7.530341629008683E-5 0.0 1.558366548415202 0.0 66-67 0.0 7.530341629008683E-5 0.0 1.7432740871155101 0.0 68-69 0.0 7.530341629008683E-5 0.0 1.9372180357706288 0.0 70-71 0.0 7.530341629008683E-5 0.0 2.1762310790753645 0.0 72-73 0.0 7.530341629008683E-5 0.0 2.4214943059321774 0.0 74-75 0.0 7.530341629008683E-5 0.0 2.6725558958433266 0.0 76-77 0.0 7.530341629008683E-5 0.0 2.9465850277229526 0.0 78-79 0.0 7.530341629008683E-5 0.0 3.2676411430757377 0.0 80-81 0.0 7.530341629008683E-5 0.0 3.632034374503468 0.0 82-83 0.0 7.530341629008683E-5 0.0 4.008024332039872 0.0 84-85 0.0 7.530341629008683E-5 0.0 4.37539204841106 0.0 86-87 0.0 7.530341629008683E-5 0.0 4.762715170099122 0.0 88-89 0.0 7.530341629008683E-5 0.0 5.191116305373425 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGCT 30 1.3684858E-6 79.15672 7 AGAGCGC 30 1.3684858E-6 79.15672 6 ATGTATT 40 9.044197E-8 71.27057 1 AGCGCTT 35 3.4198565E-6 67.84862 8 ACCTTAA 115 0.0 66.10603 1 GCGCTTA 40 7.551742E-6 59.36754 9 AAAGACA 160 0.0 50.466206 4 TACCTGG 130 0.0 43.843945 2 TGGGTAT 265 0.0 43.013462 6 TAACTTT 1540 0.0 42.251183 6 GTTATAT 120 8.731149E-11 39.594757 1 ATGGGTA 385 0.0 39.47556 5 TGGGATA 85 3.6448546E-7 39.112736 6 TGGGCGA 50 0.0016169042 37.995228 6 CAGAGAG 50 0.0016169042 37.995228 9 GACATAT 345 0.0 37.184647 1 AGGGTCT 170 0.0 36.318966 5 ACCTGGG 160 1.8189894E-12 35.623203 3 ACTTTAT 1885 0.0 34.77017 8 ATCTATA 1895 0.0 34.099552 1 >>END_MODULE