##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031670_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 719449 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20587004777267 34.0 31.0 34.0 30.0 34.0 2 32.41749032940486 34.0 31.0 34.0 31.0 34.0 3 32.52581906431172 34.0 31.0 34.0 31.0 34.0 4 35.66626404373347 37.0 35.0 37.0 35.0 37.0 5 35.4906004456188 37.0 35.0 37.0 33.0 37.0 6 35.71288583346422 37.0 36.0 37.0 35.0 37.0 7 35.81289709208019 37.0 37.0 37.0 35.0 37.0 8 35.866934278871746 37.0 37.0 37.0 35.0 37.0 9 37.77127635176364 39.0 39.0 39.0 35.0 39.0 10-11 37.73739347750848 39.0 38.5 39.0 35.0 39.0 12-13 37.80091361583656 39.0 39.0 39.0 35.0 39.0 14-15 39.21298104521655 41.0 39.0 41.0 36.0 41.0 16-17 39.183772581517246 41.0 39.0 41.0 36.0 41.0 18-19 39.19959232690573 41.0 39.0 41.0 36.0 41.0 20-21 39.248060668650595 41.0 39.0 41.0 36.0 41.0 22-23 39.14730578539966 41.0 39.0 41.0 36.0 41.0 24-25 39.0993301818475 41.0 39.0 41.0 36.0 41.0 26-27 38.99567238261503 41.0 39.0 41.0 35.0 41.0 28-29 38.90253930438433 41.0 39.0 41.0 35.0 41.0 30-31 38.723247235036816 40.0 38.0 41.0 35.0 41.0 32-33 38.53579683896982 40.0 38.0 41.0 35.0 41.0 34-35 38.4385891147253 40.0 38.0 41.0 35.0 41.0 36-37 38.2878014980909 40.0 38.0 41.0 34.0 41.0 38-39 38.096724715719944 40.0 38.0 41.0 34.0 41.0 40-41 38.02011608884021 40.0 38.0 41.0 34.0 41.0 42-43 37.84712467457735 40.0 38.0 41.0 33.0 41.0 44-45 37.60448065116499 40.0 38.0 41.0 33.0 41.0 46-47 37.31410982571384 40.0 37.5 41.0 31.5 41.0 48-49 37.33461718620778 40.0 38.0 41.0 31.5 41.0 50-51 37.081882107001334 39.5 37.5 40.5 31.5 41.0 52-53 37.22753871365448 40.0 37.5 41.0 31.5 41.0 54-55 37.515800981028534 40.0 38.0 41.0 32.0 41.0 56-57 37.449630898090064 40.0 38.0 41.0 32.0 41.0 58-59 37.31380542609692 40.0 38.0 41.0 31.0 41.0 60-61 37.106466893414265 40.0 37.5 41.0 31.0 41.0 62-63 36.800680798777954 40.0 37.0 41.0 30.0 41.0 64-65 36.59345554723129 39.5 36.5 41.0 29.0 41.0 66-67 36.39299936479166 39.0 36.0 41.0 29.0 41.0 68-69 36.109114753095774 39.0 36.0 41.0 29.0 41.0 70-71 35.793984007205516 38.5 35.0 40.5 29.0 41.0 72-73 35.41726793699067 37.0 35.0 40.0 28.5 41.0 74-75 34.93495369372951 37.0 35.0 39.0 28.0 41.0 76-77 34.50467441055586 36.5 35.0 39.0 27.5 41.0 78-79 34.117661571563794 36.0 34.5 38.5 26.5 40.0 80-81 33.72791539080602 36.0 34.0 37.0 26.0 39.0 82-83 33.33002825773613 35.0 34.0 37.0 26.0 39.0 84-85 32.95835354556056 35.0 34.0 36.0 26.0 38.0 86-87 32.695943006384056 35.0 34.0 36.0 25.5 37.0 88-89 32.38295626236189 35.0 34.0 36.0 24.5 37.0 90-91 32.162265845112024 35.0 34.0 35.5 24.0 36.5 92-93 32.00083049667175 35.0 34.0 35.0 24.0 36.0 94-95 31.822872086833115 35.0 33.5 35.0 22.5 36.0 96-97 31.694561393510867 35.0 33.0 35.0 20.5 36.0 98-99 31.59351879007407 35.0 33.0 35.0 20.0 35.5 100-101 31.180880090180125 34.5 32.0 35.0 19.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33874875419206063 1101 2 0.25033133444645017 1101 3 0.48345497642738877 1101 4 0.3138973097230888 1101 5 0.039625856502105705 1101 6 0.24234769173219917 1101 7 0.17428101101901916 1101 8 0.24782109545166975 1101 9 0.24777291510886812 1101 10-11 0.25006904177787703 1101 12-13 0.40686838841957496 1101 14-15 0.4777397884732224 1101 16-17 0.3655174264593697 1101 18-19 0.5046216419093241 1101 20-21 0.22407943017891796 1101 22-23 0.36485411261307377 1101 24-25 0.3379721262147015 1101 26-27 0.4478334578749852 1101 28-29 0.4573238352201656 1101 30-31 0.5432194641578647 1101 32-33 0.5370551465787301 1101 34-35 0.4260684319067991 1101 36-37 0.5379700481320739 1101 38-39 0.46744826207756773 1101 40-41 0.42253901087058665 1101 42-43 0.5811092989077054 1101 44-45 0.6470126698655037 1101 46-47 0.7947618756548422 1101 48-49 0.9182055915944858 1101 50-51 0.734910366006396 1101 52-53 0.5989644356441275 1101 54-55 0.4840854176304745 1101 56-57 0.7190958601632218 1101 58-59 0.6568673023190499 1101 60-61 0.41023235847058004 1101 62-63 0.4409115754583084 1101 64-65 0.4967218908809343 1101 66-67 0.6238026679967419 1101 68-69 0.5663159639143487 1101 70-71 0.5927804616711754 1101 72-73 0.7020748178203675 1101 74-75 0.7304546030081411 1101 76-77 0.5969440249244684 1101 78-79 0.43076898649660933 1101 80-81 0.4440728647176897 1101 82-83 0.5698349633973336 1101 84-85 0.5861591246401261 1101 86-87 0.5194669942697416 1101 88-89 0.595128303216903 1101 90-91 0.5165881215572696 1101 92-93 0.4434786620644786 1101 94-95 0.14871057843950197 1101 96-97 0.33857530879456377 1101 98-99 0.4075871688108599 1101 100-101 0.38508743362064735 1102 1 0.1260859783503676 1102 2 0.24002344742832804 1102 3 0.20206195711921993 1102 4 0.1809954460859018 1102 5 0.17771015504394683 1102 6 0.016099018831411627 1102 7 -0.01720216106301109 1102 8 0.21939268083288255 1102 9 0.2308340306519696 1102 10-11 0.21574740691161765 1102 12-13 0.2935461117471263 1102 14-15 0.20514161312217993 1102 16-17 0.209651497969773 1102 18-19 0.43501866200925576 1102 20-21 0.19259793758967447 1102 22-23 0.12335954397280346 1102 24-25 0.3428246607492653 1102 26-27 0.15413965340393787 1102 28-29 0.13508015281225028 1102 30-31 0.23924026587574332 1102 32-33 0.2906045746514465 1102 34-35 0.14804039637352417 1102 36-37 0.06933907468604872 1102 38-39 0.0934956957526012 1102 40-41 0.11490091757476506 1102 42-43 0.19848785058510288 1102 44-45 -0.0770031773486366 1102 46-47 -0.41557210348345563 1102 48-49 0.3554525430151543 1102 50-51 0.32859157428149643 1102 52-53 0.41516344354374013 1102 54-55 0.37533975881942894 1102 56-57 0.2504049048317256 1102 58-59 0.295663885750848 1102 60-61 0.007881368469561778 1102 62-63 -0.5579757605662152 1102 64-65 -0.23979568081240643 1102 66-67 0.17229502330543767 1102 68-69 0.2783849190898806 1102 70-71 0.2815644489070266 1102 72-73 0.21975897720423632 1102 74-75 0.13204532261081425 1102 76-77 0.15438717709251648 1102 78-79 0.30742823540344233 1102 80-81 0.08932199947899022 1102 82-83 0.19363307636721316 1102 84-85 0.16762202284487415 1102 86-87 0.4716914224924196 1102 88-89 0.24913179416264342 1102 90-91 0.3237238665622826 1102 92-93 0.27179850947056394 1102 94-95 0.2648145150506416 1102 96-97 0.40330476050015207 1102 98-99 0.1899833851535213 1102 100-101 0.21335329732980668 1103 1 0.15681694634594834 1103 2 0.0512531989482099 1103 3 0.08849383732912486 1103 4 0.2675048160825426 1103 5 0.18982932977771583 1103 6 0.19683539971650532 1103 7 0.17700376804523188 1103 8 0.2416567500722877 1103 9 0.3168306005893555 1103 10-11 0.32172038804626624 1103 12-13 0.23612247622262572 1103 14-15 0.36173508232853635 1103 16-17 0.36845143187554186 1103 18-19 0.246581733567659 1103 20-21 0.06332674042945285 1103 22-23 0.14232887283147733 1103 24-25 0.27137167027025555 1103 26-27 0.17039683972698327 1103 28-29 0.21367815745024643 1103 30-31 0.14933217104191954 1103 32-33 0.2711609057379576 1103 34-35 0.2647964231986677 1103 36-37 0.4366705771332491 1103 38-39 0.17901792563517205 1103 40-41 0.2186916495683633 1103 42-43 0.03520091616083931 1103 44-45 0.1056006504189142 1103 46-47 0.4508876388630014 1103 48-49 0.3233712015302004 1103 50-51 0.34317076485509546 1103 52-53 0.19606534489241056 1103 54-55 0.22672033053451912 1103 56-57 0.1300663998148366 1103 58-59 0.12839277975508168 1103 60-61 0.20633071806815906 1103 62-63 0.2724013753884762 1103 64-65 0.3104330849918 1103 66-67 0.01897065447684554 1103 68-69 0.05970418270899813 1103 70-71 0.05706628074279507 1103 72-73 0.1235505638645975 1103 74-75 0.2498953228957035 1103 76-77 -0.253654952281245 1103 78-79 -0.04024450699429849 1103 80-81 -0.041493295637444305 1103 82-83 -0.14661688517278293 1103 84-85 -0.037564949754504084 1103 86-87 0.005385235274822264 1103 88-89 -0.016638232104121187 1103 90-91 -0.004432560246904416 1103 92-93 -0.17786446693493474 1103 94-95 -0.16150181421257415 1103 96-97 0.12026476779330508 1103 98-99 0.10158947493911441 1103 100-101 0.11084741437356982 1104 1 0.11533154121461564 1104 2 0.3255930383682468 1104 3 0.1851735485333137 1104 4 0.3968561043092862 1104 5 0.02554906730471629 1104 6 0.09409281042525208 1104 7 0.20721297509737013 1104 8 0.44035625193457406 1104 9 0.3499957304099226 1104 10-11 0.42324932291617756 1104 12-13 0.39481453433779023 1104 14-15 0.47050548857912844 1104 16-17 0.41252192092433404 1104 18-19 0.3824732706205225 1104 20-21 0.45296779539182097 1104 22-23 0.4294943424752873 1104 24-25 0.5156951388406483 1104 26-27 0.46510719435681125 1104 28-29 0.4870929225002385 1104 30-31 0.6085439429503978 1104 32-33 0.4003544188899255 1104 34-35 0.6848138998223376 1104 36-37 0.6333432761118942 1104 38-39 0.576037999479297 1104 40-41 0.7047022596050141 1104 42-43 0.6826194732066426 1104 44-45 0.6704219330628476 1104 46-47 0.8098920107159273 1104 48-49 0.7000962764396448 1104 50-51 0.8045064320009274 1104 52-53 0.793265578992866 1104 54-55 0.8401390332188541 1104 56-57 0.8148722555625696 1104 58-59 0.7870335946835709 1104 60-61 0.7919311129103903 1104 62-63 0.9146929167565716 1104 64-65 0.9908333751900642 1104 66-67 0.848912048453343 1104 68-69 0.5615759075276898 1104 70-71 0.6353415968997709 1104 72-73 0.7954080754415074 1104 74-75 0.586256948761104 1104 76-77 0.6682720072986683 1104 78-79 0.682419275669865 1104 80-81 0.5871689065789596 1104 82-83 0.7412009911477426 1104 84-85 0.8168787784839893 1104 86-87 0.739620538594032 1104 88-89 0.912176890569377 1104 90-91 0.7107416261326591 1104 92-93 0.7466974712442038 1104 94-95 0.7410174626651518 1104 96-97 0.7883114044872066 1104 98-99 0.5546183518233114 1104 100-101 0.6196868640756854 1105 1 0.06256328369947539 1105 2 0.15577839410732963 1105 3 0.1877402448931349 1105 4 0.22591161037625085 1105 5 0.06298760499237943 1105 6 0.06913294651049284 1105 7 0.2149382278666323 1105 8 0.2549577345791718 1105 9 0.3879954183801644 1105 10-11 0.33943033036966597 1105 12-13 0.14530522628893294 1105 14-15 0.3504532990864746 1105 16-17 0.32830534207894146 1105 18-19 0.1184684190611307 1105 20-21 0.18837159712207097 1105 22-23 0.20307523618890144 1105 24-25 0.056781153365129455 1105 26-27 0.12626175477231527 1105 28-29 0.0725941077100245 1105 30-31 0.22206112220143126 1105 32-33 0.34018300383500133 1105 34-35 0.3468139602151936 1105 36-37 0.21566880366864893 1105 38-39 0.17356491204554914 1105 40-41 0.006592304346050071 1105 42-43 0.10169933772533568 1105 44-45 0.16080688718944458 1105 46-47 0.34431593836528407 1105 48-49 0.3767038587626601 1105 50-51 0.2561669838909708 1105 52-53 0.12419452176559531 1105 54-55 0.3567332284588858 1105 56-57 0.3104774171386211 1105 58-59 0.16231354073261883 1105 60-61 0.14133272036689704 1105 62-63 0.398131434313278 1105 64-65 0.43260711335683766 1105 66-67 0.3398468292021093 1105 68-69 0.34099855182886785 1105 70-71 0.2979116148666492 1105 72-73 0.29791437894580497 1105 74-75 0.37625337551715177 1105 76-77 0.5405763470286189 1105 78-79 0.44769489356426817 1105 80-81 0.6478448536144228 1105 82-83 0.4210660131156416 1105 84-85 0.43963354826139067 1105 86-87 0.39671242129097095 1105 88-89 0.3730247056061913 1105 90-91 0.2545075736210691 1105 92-93 0.2597574262515252 1105 94-95 0.5404729204178373 1105 96-97 0.5126633136563505 1105 98-99 0.3275839933212552 1105 100-101 0.6355966069531043 1106 1 0.357062170429586 1106 2 0.32962813339890573 1106 3 0.23649721169358173 1106 4 0.3603809044544093 1106 5 0.33236582839326445 1106 6 0.18956227632673972 1106 7 0.23329890840308565 1106 8 0.3660649063063701 1106 9 0.38904250662394446 1106 10-11 0.3369544275810483 1106 12-13 0.3646371225350151 1106 14-15 0.35109266109537174 1106 16-17 0.4509080299993755 1106 18-19 0.43224271127202485 1106 20-21 0.35022118514130085 1106 22-23 0.471261008079658 1106 24-25 0.449988788836599 1106 26-27 0.22999794526200645 1106 28-29 0.258793815565312 1106 30-31 0.28173924302075903 1106 32-33 0.30213564613736565 1106 34-35 0.3573191152929809 1106 36-37 0.33533704451312474 1106 38-39 0.3865540062551247 1106 40-41 0.37881562959577053 1106 42-43 0.29128021879218124 1106 44-45 0.5629194999937042 1106 46-47 0.4253506411860428 1106 48-49 0.5981488405390749 1106 50-51 0.5384671743995781 1106 52-53 0.36981471473237804 1106 54-55 0.5645329722413592 1106 56-57 0.5772355562997191 1106 58-59 0.7818009638555381 1106 60-61 0.713857172122843 1106 62-63 0.5929406657079568 1106 64-65 0.7413941455352955 1106 66-67 0.6672273595062137 1106 68-69 0.5520801963483208 1106 70-71 0.559884584389998 1106 72-73 0.5471308354857385 1106 74-75 0.5975789939610365 1106 76-77 0.5263425650782381 1106 78-79 0.6547276392990895 1106 80-81 0.4515654441438315 1106 82-83 0.38779555234954444 1106 84-85 0.1835306547051232 1106 86-87 0.12370838893759384 1106 88-89 0.19248116517156433 1106 90-91 0.36063339439170505 1106 92-93 0.4532607518794265 1106 94-95 0.1267808825430201 1106 96-97 0.27665718712649223 1106 98-99 0.3410046864322176 1106 100-101 0.26246328401974495 1107 1 -0.08535427452191868 1107 2 0.023607418067719266 1107 3 0.1382641339584154 1107 4 0.14219749689226546 1107 5 0.04597559028574949 1107 6 0.16355491994100646 1107 7 0.03494770710663886 1107 8 0.10765448632891861 1107 9 0.2804405561282479 1107 10-11 0.24801433438223341 1107 12-13 0.23848362466549844 1107 14-15 0.24147309965430708 1107 16-17 0.22345965582338323 1107 18-19 0.3454824124440705 1107 20-21 0.108352546610611 1107 22-23 0.09608981857315513 1107 24-25 0.20530266801883812 1107 26-27 0.2311948709114091 1107 28-29 0.17579128050368809 1107 30-31 0.2688050492757057 1107 32-33 0.088939428781039 1107 34-35 0.17028267182082146 1107 36-37 0.20408204331396007 1107 38-39 0.2790122539470019 1107 40-41 0.19569048810526368 1107 42-43 0.19824838819078394 1107 44-45 0.14627281944508752 1107 46-47 0.3127376449045798 1107 48-49 0.3059443231533905 1107 50-51 0.048134478380589485 1107 52-53 -0.021584455488827814 1107 54-55 0.2229597546855544 1107 56-57 0.07894352885560352 1107 58-59 0.06469232380067069 1107 60-61 -0.026685658314939076 1107 62-63 -0.1772204709679528 1107 64-65 -0.03948664829088955 1107 66-67 -0.08898869678067456 1107 68-69 0.025905853144458035 1107 70-71 0.1296873158012275 1107 72-73 0.15417274805637504 1107 74-75 0.16623299054957386 1107 76-77 0.004578242876611682 1107 78-79 -0.07631151182509655 1107 80-81 0.03170230609168101 1107 82-83 -0.012671338311072589 1107 84-85 0.05041526345164726 1107 86-87 -0.015342319019261197 1107 88-89 -0.07859895936250183 1107 90-91 0.2329411522182383 1107 92-93 0.005274343933990622 1107 94-95 0.13993361608361354 1107 96-97 0.038036386172535686 1107 98-99 -0.01922734679479632 1107 100-101 -0.026391410551276806 1108 1 0.1301882902996141 1108 2 0.2285119069643997 1108 3 0.17931846972020793 1108 4 0.09917577427086144 1108 5 0.16354729663396483 1108 6 0.1977432216042132 1108 7 0.2311877658570225 1108 8 0.10129333835973142 1108 9 0.19752865667152264 1108 10-11 0.11728950984058883 1108 12-13 0.2295625788418647 1108 14-15 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-1.576672609707643 2316 96-97 -1.3073553972220004 2316 98-99 -0.9751802013876194 2316 100-101 -1.7049679021756816 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 2.0 7 38.0 8 151.0 9 202.0 10 231.0 11 297.0 12 368.0 13 460.0 14 637.0 15 889.0 16 1401.0 17 2200.0 18 2951.0 19 4015.0 20 5066.0 21 6837.0 22 7039.0 23 5819.0 24 4628.0 25 4328.0 26 4469.0 27 5117.0 28 5800.0 29 6837.0 30 8307.0 31 10078.0 32 12699.0 33 16520.0 34 22796.0 35 35377.0 36 57431.0 37 124526.0 38 264907.0 39 96980.0 40 45.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.669177038478246 25.18565903545295 14.947141123214468 21.19802280285434 2 13.225407268826025 23.933469775540015 43.18814375818758 19.65297919744638 3 14.318502456028934 32.258864196292905 33.651147794866226 19.771485552811935 4 9.064465573085362 20.768989656253265 46.46856824434925 23.69797652631212 5 8.658465894523282 42.97978418629538 38.70503416678743 9.656715752393904 6 22.380277257065824 46.47676850956918 20.390956417105595 10.751997816259397 7 22.86236707683224 36.13114900206661 27.286083522262018 13.720400398839134 8 22.483723845002263 38.50614490129861 23.214845245970128 15.795286007728999 9 25.871526670463833 16.69871977874301 25.14166268613899 32.288090864654166 10-11 20.44143106452381 29.40473520960803 33.65177169932993 16.502062026538233 12-13 21.32164583941138 29.424953296211687 31.575869024682856 17.677531839694076 14-15 20.548343944742218 30.528198168147945 29.035892629208384 19.887565257901453 16-17 17.460013828391606 33.39813942747119 30.44089475536685 18.700951988770345 18-19 17.17272281684853 32.67025644056189 33.51914874386882 16.63787199872075 20-21 18.130800462021522 31.93105260012204 33.269094179279364 16.669052758577074 22-23 18.817178146053436 31.047857457582122 32.497994993390776 17.636969402973666 24-25 18.348208142620255 31.71983003659745 32.35524686252952 17.576714958252772 26-27 17.765192529282825 31.6881391175747 33.20492488001234 17.34174347313013 28-29 18.02129129375397 29.889679463033513 34.37123409720495 17.71779514600757 30-31 18.380316047419623 31.54038715739406 32.80461853446179 17.274678260724528 32-33 17.477958826824416 32.25857565998424 32.88975313052072 17.37371238267063 34-35 17.750945515248475 31.620100938356995 33.29610576983219 17.33284777656234 36-37 18.898351377234523 30.270387477083155 32.11999738688913 18.711263758793187 38-39 17.458360495323504 32.52697550486553 32.721777360174244 17.292886639636723 40-41 16.86505923282957 31.475684864389276 34.39534977461919 17.263906128161967 42-43 17.492136343229333 31.398681490974344 32.04619090442825 19.062991261368076 44-45 17.95932002853514 31.812851251892194 32.32425660745045 17.903572112122216 46-47 17.832117911791805 31.036951554232918 32.79179693185863 18.33913360211665 48-49 17.10274347554202 31.5380393689888 32.57905899227131 18.78015816319788 50-51 17.702373037883486 30.952900858699795 31.265838101569692 20.078888001847027 52-53 17.689928130602844 31.73965112672354 31.98994913365872 18.5804716090149 54-55 18.365943110076167 31.105978122898204 31.562800163309706 18.96527860371592 56-57 18.300241146929864 31.812122468687775 30.874321121994186 19.013315262388176 58-59 17.818957637411636 31.013076487504364 31.42016024575282 19.74780562933118 60-61 19.15604707650977 32.864053006312595 29.978911616706455 18.00098830047118 62-63 17.906171036763993 32.20191737628353 31.939481635419835 17.95242995153264 64-65 17.984124531815656 30.79048863761314 31.614256465502244 19.61113036506896 66-67 18.400003895947588 31.43180943807217 30.64816740886787 19.520019257112363 68-69 18.11745700226042 31.46110566510568 31.01145655560294 19.409980777030952 70-71 18.585569104277237 30.56691314055968 32.60981768351639 18.237700071646692 72-73 18.728644107286303 30.883446292516183 30.948335518331383 19.43957408186614 74-75 17.966676850634595 31.81594531000439 30.508685718342072 19.708692121018945 76-77 17.833199846780655 31.500853153184526 32.12375944562454 18.54218755441028 78-79 17.455722189359406 31.549671178537874 31.21583910365705 19.778767528445666 80-81 18.262222648853868 31.732212590549093 30.36842888722874 19.637135873368308 82-83 18.823489312921875 31.422193842061624 30.78405758390233 18.970259261114172 84-85 17.606820839568215 31.186794640888166 30.983521823305693 20.22286269623792 86-87 17.531289729401912 31.85334791063336 31.62342160389247 18.99194075607225 88-89 18.970658923815044 30.58465810714268 31.095252239762207 19.34943072928007 90-91 18.006417892170507 30.6306149598673 32.4591993498614 18.903767798100795 92-93 19.096310270055795 31.876398745610157 30.838499048069863 18.188791936264188 94-95 18.440231562378003 29.990654277146962 31.868288633221088 19.70082552725395 96-97 19.51041532612892 29.972070439534065 31.12097360430456 19.39654063003245 98-99 18.17817603374248 30.908880979722657 31.116502389558974 19.79644059697589 100-101 18.39883225729283 30.29107336083472 31.618900853136466 19.691193528735983 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 484.0 1 305.5 2 224.5 3 437.5 4 381.5 5 293.0 6 561.5 7 1013.5 8 1362.0 9 1417.5 10 1163.0 11 908.5 12 889.0 13 916.5 14 977.0 15 1005.0 16 955.0 17 943.0 18 967.5 19 1030.5 20 1165.0 21 1356.0 22 1615.0 23 1872.5 24 2133.0 25 2806.0 26 4729.0 27 6769.0 28 7110.5 29 8984.5 30 14406.5 31 19482.0 32 28968.5 33 39640.0 34 48244.0 35 54511.5 36 59674.0 37 62157.5 38 52985.0 39 44447.0 40 37798.0 41 31653.5 42 26490.0 43 23460.0 44 23290.0 45 21133.5 46 17017.0 47 12163.0 48 10419.5 49 8701.5 50 6790.0 51 4728.5 52 2657.5 53 1908.5 54 1626.0 55 1703.5 56 1649.0 57 1331.5 58 1072.5 59 866.0 60 712.0 61 654.0 62 583.5 63 462.0 64 396.5 65 333.0 66 225.5 67 124.5 68 83.0 69 77.0 70 57.0 71 35.5 72 33.5 73 33.0 74 29.5 75 31.0 76 19.5 77 13.0 78 10.0 79 6.5 80 5.5 81 3.5 82 1.5 83 2.0 84 1.5 85 1.5 86 1.5 87 0.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.48453747242681555 2 0.3700053791165183 3 0.19737326759784224 4 0.22572830040767308 5 0.24921849915699373 6 0.19667829130348363 7 0.18972852835989765 8 0.19097948568974313 9 0.19042350465425623 10-11 0.20376704950594138 12-13 0.1889640544361032 14-15 0.19911070833373876 16-17 0.08867897516015728 18-19 0.03843218907803055 20-21 2.779905177434398E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.14406858582053766 46-47 0.2116202816321935 48-49 0.08819249175410626 50-51 0.06324284278663254 52-53 0.07033160098909026 54-55 0.07915779992744448 56-57 0.11119620709737592 58-59 0.09423878551502608 60-61 0.14511105026207555 62-63 0.24317220539607393 64-65 0.1364933442120289 66-67 0.10473292755984094 68-69 0.1080688137727622 70-71 0.08951294671338761 72-73 0.07387598009031912 74-75 0.138925761242284 76-77 0.21182877452050108 78-79 0.13538138214105516 80-81 0.08895696567790072 82-83 0.08853997990128556 84-85 0.09875613142835697 86-87 0.07269452038990949 88-89 0.09430828314446195 90-91 0.11578305064014267 92-93 0.16283294576821986 94-95 0.1304470504511091 96-97 0.0940302926267185 98-99 0.08235469088149402 100-101 0.06324284278663254 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 719449.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.32447867938555 #Duplication Level Percentage of deduplicated Percentage of total 1 74.88227028277588 11.475317544123852 2 8.685961839622042 2.6621567404248894 3 3.2687464940355215 1.5027550786849087 4 1.8777314742093956 1.1510102376853233 5 1.3333352728627899 1.021633398072927 6 0.9733252324376918 0.8949421063559636 7 0.7782939058243797 0.8348863856270989 8 0.5659489338150983 0.6938297895896366 9 0.46570031622672053 0.6422953110299539 >10 4.928585714568014 16.51714269313266 >50 1.0307378777571314 11.192474084412154 >100 1.065369212288317 32.043957916045194 >500 0.11483021449814196 11.913956485680833 >1k 0.029163229078893193 7.453642229134609 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4103 0.5702975471506667 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3450 0.47953364310743357 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 3014 0.41893171023936376 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 2808 0.3902986869117894 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2489 0.3459591993317108 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 2234 0.3105154083194222 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2200 0.30578956951778374 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1917 0.266453911257087 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1832 0.2546393142529908 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 1787 0.24838452760376342 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1683 0.23392902068110458 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1627 0.22614528618428825 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1513 0.2102998266729122 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1422 0.19765125811558568 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1381 0.19195245250184517 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1371 0.19056249991312796 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1364 0.18958953310102591 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1320 0.18347374171067024 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 1257 0.1747170404017519 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1250 0.17374407358964986 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1239 0.17221512574206094 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 1172 0.16290244339765572 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 1165 0.16192947658555365 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 1162 0.1615124908089385 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1158 0.16095650977345163 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1118 0.15539669941858283 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 1079 0.14997588432258574 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 1073 0.14914191276935546 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1068 0.14844693647499682 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1060 0.14733497440402307 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 1044 0.14511105026207555 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 1010 0.1403852114604371 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 993 0.13802229205961786 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 989 0.13746631102413098 No Hit GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG 986 0.1370493252475158 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 978 0.13593736317654204 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 972 0.13510339162331172 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 958 0.13315745799910766 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 950 0.1320454959281339 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 947 0.13162851015151872 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 945 0.13135051963377528 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 942 0.13093353385716014 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 940 0.1306555433394167 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 936 0.13009956230392983 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 929 0.12912659549182776 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 926 0.12870960971521261 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 915 0.1271806618676237 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 893 0.12412276617244586 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 884 0.12287180884260039 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 883 0.12273281358372864 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 882 0.12259381832485693 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 881 0.12245482306598521 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 851 0.11828496529983364 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 845 0.1174509937466033 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 840 0.1167560174522447 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 833 0.11578305064014267 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 820 0.11397611227481032 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 804 0.11175218813286279 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 798 0.11091821657963245 No Hit TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA 777 0.10799931614332635 No Hit CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT 774 0.10758233036671119 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 774 0.10758233036671119 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 774 0.10758233036671119 No Hit ACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTAT 753 0.10466342993040507 No Hit CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC 751 0.10438543941266164 No Hit CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG 749 0.1041074488949182 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 745 0.10355146785943131 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 741 0.10299548682394444 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 723 0.10049357216425348 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.389952588717199E-4 0.0 9 0.0 0.0 0.0 1.389952588717199E-4 0.0 10-11 0.0 0.0 0.0 6.949762943585995E-4 0.0 12-13 0.0 0.0 0.0 0.002501914659690958 0.0 14-15 0.0 0.0 0.0 0.004308853025023316 0.0 16-17 0.0 0.0 0.0 0.00986866337989211 0.0 18-19 0.0 0.0 0.0 0.016123450029119506 0.0 20-21 0.0 0.0 0.0 0.030231468804599072 0.0 22-23 0.0 0.0 0.0 0.056015089325303115 0.0 24-25 0.0 0.0 0.0 0.0827716766581092 0.0 26-27 0.0 0.0 0.0 0.0954202452154357 0.0 28-29 0.0 0.0 0.0 0.10660936355460915 0.0 30-31 0.0 0.0 0.0 0.12433125906075344 0.0 32-33 0.0 0.0 0.0 0.14830794121612512 0.0 34-35 0.0 0.0 0.0 0.18952003547159008 0.0 36-37 0.0 0.0 0.0 0.24574361768520075 0.0 38-39 0.0 0.0 0.0 0.3306002232263857 0.0 40-41 0.0 0.0 0.0 0.42608996607125726 0.0 42-43 0.0 0.0 0.0 0.5148384388608505 0.0 44-45 0.0 0.0 0.0 0.6093552148936199 0.0 46-47 0.0 0.0 0.0 0.7216633840619697 0.0 48-49 0.0 0.0 0.0 0.8443961976456983 0.0 50-51 0.0 0.0 0.0 0.9767891817210115 0.0 52-53 0.0 0.0 0.0 1.0978540521982796 0.0 54-55 0.0 0.0 0.0 1.225104211695339 0.0 56-57 0.0 0.0 0.0 1.3692422951453125 0.0 58-59 0.0 0.0 0.0 1.5275578950002016 0.0 60-61 0.0 0.0 0.0 1.7028309164374402 0.0 62-63 0.0 0.0 0.0 1.877200468692013 0.0 64-65 0.0 0.0 0.0 2.057685812336941 0.0 66-67 0.0 0.0 0.0 2.2585339614065765 0.0 68-69 0.0 0.0 0.0 2.491073029498964 0.0 70-71 0.0 0.0 0.0 2.762391774816561 0.0 72-73 0.0 0.0 0.0 3.0326680556926204 0.0 74-75 0.0 0.0 0.0 3.2955775878484785 0.0 76-77 0.0 0.0 0.0 3.5833672713423743 0.0 78-79 0.0 0.0 0.0 3.9152879495280413 0.0 80-81 0.0 0.0 0.0 4.298636873496244 0.0 82-83 0.0 0.0 0.0 4.683862233459217 0.0 84-85 0.0 0.0 0.0 5.049628257180148 0.0 86-87 0.0 0.0 0.0 5.434158640848761 0.0 88-89 0.0 0.0 0.0 5.838078863129979 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACT 20 0.0020531514 71.4296 1 TGGTACT 100 0.0 57.064026 2 TACCTGG 135 0.0 49.314594 2 CCCGTGT 20 5.336826E-4 47.593163 46-47 TGGGTAC 110 0.0 47.50369 6 ATGTATT 165 0.0 46.176716 1 TACTCCC 120 1.8189894E-12 43.554146 5 GGTACTC 120 1.8189894E-12 43.55111 3 ACCTGGG 155 0.0 42.912533 3 ACTCCCG 125 3.6379788E-12 41.803246 6 GTGGTAC 145 0.0 39.40944 1 CATGGGT 410 0.0 39.401535 4 CTCCCGC 135 7.2759576E-12 38.704014 7 GACATAT 160 0.0 38.691036 1 GGTATCA 2405 0.0 38.016586 1 ATGGGTA 350 0.0 38.01089 5 CCTGGCT 50 0.0016153483 38.000305 8 TGGGTAT 205 0.0 37.076054 6 CATTCCG 205 0.0 37.07347 9 GGGATGC 195 0.0 36.538754 7 >>END_MODULE