##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031670_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 719449 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53236435105199 34.0 31.0 34.0 31.0 34.0 2 32.731291585644016 34.0 31.0 34.0 31.0 34.0 3 32.809908694014446 34.0 31.0 34.0 31.0 34.0 4 36.26656927732195 37.0 37.0 37.0 35.0 37.0 5 36.03305863237005 37.0 35.0 37.0 35.0 37.0 6 36.10706387805112 37.0 37.0 37.0 35.0 37.0 7 36.048124328479155 37.0 37.0 37.0 35.0 37.0 8 36.14680957232549 37.0 37.0 37.0 35.0 37.0 9 38.016541825758324 39.0 39.0 39.0 35.0 39.0 10-11 37.99012994666752 39.0 38.5 39.0 35.0 39.0 12-13 38.03354233587092 39.0 39.0 39.0 35.0 39.0 14-15 39.448083880858825 41.0 39.0 41.0 36.5 41.0 16-17 39.41347892623383 41.0 39.0 41.0 36.5 41.0 18-19 39.513965548635134 41.0 39.5 41.0 37.0 41.0 20-21 39.55666975699459 41.0 40.0 41.0 37.0 41.0 22-23 39.45219466563995 41.0 39.0 41.0 36.0 41.0 24-25 39.40143012221853 41.0 39.0 41.0 36.0 41.0 26-27 39.28826782718441 41.0 39.0 41.0 36.0 41.0 28-29 39.19624810097727 41.0 39.0 41.0 36.0 41.0 30-31 39.03714717790976 40.0 39.0 41.0 35.0 41.0 32-33 38.83654713537722 40.0 38.0 41.0 35.0 41.0 34-35 38.722292337608366 40.0 38.0 41.0 35.0 41.0 36-37 38.56583649431718 40.0 38.0 41.0 35.0 41.0 38-39 38.41389660698674 40.0 38.0 41.0 35.0 41.0 40-41 38.33282484234463 40.0 38.0 41.0 34.0 41.0 42-43 38.185077052021754 40.0 38.0 41.0 34.0 41.0 44-45 38.06150192716926 40.0 38.0 41.0 33.5 41.0 46-47 37.93846471396861 40.0 38.0 41.0 33.0 41.0 48-49 37.933203048444014 40.0 38.0 41.0 33.0 41.0 50-51 37.833660898826736 40.0 38.0 41.0 33.0 41.0 52-53 37.79400833137582 40.0 38.0 41.0 33.0 41.0 54-55 37.71745530259963 40.0 38.0 41.0 33.0 41.0 56-57 37.637576812254935 40.0 38.0 41.0 33.0 41.0 58-59 37.49340814984801 40.0 38.0 41.0 32.5 41.0 60-61 37.28095320168629 40.0 37.5 41.0 32.0 41.0 62-63 37.08362580252387 40.0 37.0 41.0 31.0 41.0 64-65 36.84351218779928 39.5 37.0 41.0 31.0 41.0 66-67 36.5928752420255 39.0 36.0 41.0 31.0 41.0 68-69 36.28217427503548 39.0 36.0 41.0 30.5 41.0 70-71 35.934082193456376 38.5 35.0 40.0 30.0 41.0 72-73 35.5585899764959 37.0 35.0 40.0 29.5 41.0 74-75 35.13985146966637 37.0 35.0 39.0 29.0 41.0 76-77 34.47300781570341 36.0 34.5 39.0 29.0 40.0 78-79 34.37447060180777 36.0 35.0 38.5 29.0 40.0 80-81 34.047607961092446 36.0 34.5 37.0 29.0 39.0 82-83 33.69716407973324 35.0 34.0 37.0 28.5 39.0 84-85 33.32245788096168 35.0 34.0 36.0 28.0 38.0 86-87 33.05507826128051 35.0 34.0 36.0 27.0 37.0 88-89 32.766222484151065 35.0 34.0 36.0 26.5 37.0 90-91 32.58645157613674 35.0 34.0 35.5 26.0 36.5 92-93 32.453068250841966 35.0 34.0 35.0 26.0 36.0 94-95 32.29752282649639 35.0 34.0 35.0 25.5 36.0 96-97 32.18232286096721 35.0 34.0 35.0 25.0 36.0 98-99 32.11280160233734 35.0 34.0 35.0 25.0 36.0 100-101 31.72715508673999 34.5 33.0 35.0 24.0 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2776608598153061 1101 2 0.1941397614773237 1101 3 0.27870329203738464 1101 4 0.20503189772327346 1101 5 0.39761903632801676 1101 6 0.1929043663588459 1101 7 0.34780187194296275 1101 8 0.24431639888642565 1101 9 0.28874740884265293 1101 10-11 0.23162151120369145 1101 12-13 0.1301375051412279 1101 14-15 0.24117138654280268 1101 16-17 0.15229865102416795 1101 18-19 0.22309232721870842 1101 20-21 0.2653030162787786 1101 22-23 0.2701393688799456 1101 24-25 0.24281026077569123 1101 26-27 0.23634106763373808 1101 28-29 0.39534248714625164 1101 30-31 0.24514267494267727 1101 32-33 0.1640911177471267 1101 34-35 0.13673939110379507 1101 36-37 0.48433296294578554 1101 38-39 0.439651058938324 1101 40-41 0.4315645469185583 1101 42-43 0.395148809173719 1101 44-45 0.47951534203367885 1101 46-47 0.594817157607423 1101 48-49 0.3636276434662733 1101 50-51 0.6650434165466024 1101 52-53 0.46884894753483053 1101 54-55 0.6230308662880262 1101 56-57 0.5115139708304923 1101 58-59 0.43572448956545173 1101 60-61 0.3930911190882327 1101 62-63 0.37474090687177863 1101 64-65 0.4958702055514692 1101 66-67 0.491132453047058 1101 68-69 0.430918987135982 1101 70-71 0.3745562964422362 1101 72-73 0.4575695704200129 1101 74-75 0.4486053324999233 1101 76-77 0.32375582785249435 1101 78-79 0.49385915964159466 1101 80-81 0.4424902227806271 1101 82-83 0.4477324728263383 1101 84-85 0.549380605490505 1101 86-87 0.3226185603784515 1101 88-89 0.27074293774917635 1101 90-91 0.35345740475389675 1101 92-93 0.16732029660749248 1101 94-95 0.25581368955621997 1101 96-97 0.26143007223045345 1101 98-99 0.1396524854968746 1101 100-101 0.3902898418482579 1102 1 0.08900883272748672 1102 2 0.20389581514911725 1102 3 0.17103880671195526 1102 4 0.12249682331574263 1102 5 -0.04215615156523711 1102 6 0.08785809033420833 1102 7 0.18610870327989915 1102 8 0.1659860546503822 1102 9 0.10856482398327927 1102 10-11 0.16708700062588377 1102 12-13 0.1541632493664693 1102 14-15 0.13706982521432565 1102 16-17 0.1331419363519899 1102 18-19 0.1754817056264315 1102 20-21 0.11784804767929558 1102 22-23 0.009804490013365807 1102 24-25 0.13744907985233823 1102 26-27 0.10877309316202854 1102 28-29 -0.1683612743177889 1102 30-31 -0.12282969740088134 1102 32-33 0.08677596692266576 1102 34-35 0.059529208578915416 1102 36-37 0.25190465629439274 1102 38-39 0.16187794641830777 1102 40-41 0.029029112619717523 1102 42-43 0.21461383073720697 1102 44-45 0.24112633551365548 1102 46-47 0.17778260204320873 1102 48-49 0.03270008358935428 1102 50-51 0.16189586710653714 1102 52-53 0.027866624196484224 1102 54-55 -0.035135367509369075 1102 56-57 -0.11270851397910064 1102 58-59 0.05552181077445084 1102 60-61 0.10471470876414912 1102 62-63 0.035429378953210744 1102 64-65 0.26065723986711475 1102 66-67 0.2651716931965282 1102 68-69 0.35588914614795897 1102 70-71 0.38651668440506626 1102 72-73 0.2964312341975557 1102 74-75 0.2048029608161812 1102 76-77 0.06605415375807411 1102 78-79 0.056312298793578464 1102 80-81 0.14698646491549994 1102 82-83 -0.09078339538145741 1102 84-85 0.17638774933305967 1102 86-87 0.07740811393327363 1102 88-89 0.16017532916696098 1102 90-91 0.3969472708143442 1102 92-93 0.35071191233954124 1102 94-95 0.3350347647914944 1102 96-97 0.16167719759859267 1102 98-99 0.13085764153974822 1102 100-101 0.13177841229997966 1103 1 0.15299203567666098 1103 2 0.07772136056895818 1103 3 0.010438374820346041 1103 4 0.00843299680170162 1103 5 0.10112018499113873 1103 6 0.0646795276557981 1103 7 0.08917809652346875 1103 8 0.1939336578798887 1103 9 0.18213738804055168 1103 10-11 0.056966036789681596 1103 12-13 0.05244650872364076 1103 14-15 0.12086249445258801 1103 16-17 0.17412178330965844 1103 18-19 0.18778890704859208 1103 20-21 -0.07745043044408817 1103 22-23 0.038018464384755646 1103 24-25 0.1080513920108288 1103 26-27 -0.06201354212887367 1103 28-29 0.10216639694318275 1103 30-31 -0.016858140710830583 1103 32-33 0.11914362177404314 1103 34-35 -0.0866088371970335 1103 36-37 -0.19681106444608787 1103 38-39 -0.14939453843984296 1103 40-41 -0.14837976565863897 1103 42-43 -0.19071045832249212 1103 44-45 0.14332966643492284 1103 46-47 0.11932197084330909 1103 48-49 -0.27800119898056863 1103 50-51 -0.044259727826997164 1103 52-53 -0.2789630179260527 1103 54-55 -0.2620778826227266 1103 56-57 -0.4186963093376903 1103 58-59 -0.37316906765205005 1103 60-61 -0.283100307161142 1103 62-63 -0.43907591143406677 1103 64-65 -0.3345868244722112 1103 66-67 -0.2862308149655348 1103 68-69 -0.2871920414969793 1103 70-71 -0.39896195747246566 1103 72-73 -0.16413444124558652 1103 74-75 -0.11744059642085602 1103 76-77 -0.17706862766944909 1103 78-79 -0.10872100472138158 1103 80-81 -0.0016771211522907947 1103 82-83 -0.004355218727539523 1103 84-85 -0.14238158750250562 1103 86-87 -0.3578095696918808 1103 88-89 -0.31383710338558046 1103 90-91 -0.16161164706358022 1103 92-93 -0.1346020637411769 1103 94-95 -0.025762810234006395 1103 96-97 0.0512115181669941 1103 98-99 -0.2578348322647166 1103 100-101 -0.3589128421798975 1104 1 0.16813844285501744 1104 2 0.2217794433725544 1104 3 0.25099546916984394 1104 4 0.16851401497685004 1104 5 0.17359423301409294 1104 6 0.1939633906369096 1104 7 0.2656380553670985 1104 8 0.26645085806004687 1104 9 0.20242699295558708 1104 10-11 0.20303069490322656 1104 12-13 0.2091980682695791 1104 14-15 0.24880459504566232 1104 16-17 0.3000424750387509 1104 18-19 0.323397258069825 1104 20-21 0.1734631816342045 1104 22-23 0.3106875062180805 1104 24-25 0.09735909655879027 1104 26-27 0.08417517843663802 1104 28-29 0.035319659920517665 1104 30-31 0.0390616610050003 1104 32-33 0.11906271147928749 1104 34-35 0.301021596940636 1104 36-37 0.2767258751489834 1104 38-39 0.1789124675825775 1104 40-41 0.2735498909310934 1104 42-43 0.2930225269167721 1104 44-45 0.32610871929901464 1104 46-47 0.48975087631581005 1104 48-49 0.39691876725286335 1104 50-51 0.6384613393258505 1104 52-53 0.6959912003321662 1104 54-55 0.5178070089979343 1104 56-57 0.5015625496653371 1104 58-59 0.6079720911451929 1104 60-61 0.6100037084167909 1104 62-63 0.6009760329751686 1104 64-65 0.5862208760302536 1104 66-67 0.5887614311312177 1104 68-69 0.6480743286784048 1104 70-71 0.611127556283634 1104 72-73 0.43791231951075815 1104 74-75 0.4123600136427683 1104 76-77 0.3501590350209227 1104 78-79 0.3270699338650047 1104 80-81 0.39091176554902063 1104 82-83 0.4251139288285586 1104 84-85 0.35992031038330907 1104 86-87 0.2804094868401421 1104 88-89 0.41731416919850517 1104 90-91 0.3994284278054181 1104 92-93 0.56244282259334 1104 94-95 0.4495015641541187 1104 96-97 0.5217399433020518 1104 98-99 0.46007547058251674 1104 100-101 0.5941875054646744 1105 1 0.047941562146007755 1105 2 -0.006671630865362488 1105 3 -0.04836939929955264 1105 4 0.10702150620730322 1105 5 -0.023097748324509837 1105 6 -0.04987276354605541 1105 7 0.064320987842855 1105 8 -0.13206398096782834 1105 9 0.08626888445981962 1105 10-11 0.01115630197724471 1105 12-13 -0.04539178875033656 1105 14-15 -0.09401796571206233 1105 16-17 -0.07053311920179084 1105 18-19 -0.0986217254177646 1105 20-21 0.09393334001446618 1105 22-23 0.00290412532537232 1105 24-25 0.1377946297018866 1105 26-27 0.18589184535494496 1105 28-29 0.04797951225998531 1105 30-31 -0.053429997231823734 1105 32-33 0.12832169114199132 1105 34-35 -0.04879269502862371 1105 36-37 0.018892418513566156 1105 38-39 0.15500947863949932 1105 40-41 0.19982908710218794 1105 42-43 0.22303727283899377 1105 44-45 0.11825412516417089 1105 46-47 0.327554539142497 1105 48-49 0.565989824480134 1105 50-51 0.43953985507076965 1105 52-53 0.480683121277913 1105 54-55 0.11780499590275184 1105 56-57 0.16058952010806138 1105 58-59 0.15646755413192182 1105 60-61 0.3648552678106469 1105 62-63 0.3847217991843621 1105 64-65 0.35335231604756245 1105 66-67 0.24423403038939284 1105 68-69 0.3055637976742247 1105 70-71 0.2257695524688188 1105 72-73 0.42598850509801167 1105 74-75 0.2495144532725604 1105 76-77 0.15402317122693887 1105 78-79 0.2330583887641282 1105 80-81 0.15432275402908857 1105 82-83 0.10531614423981495 1105 84-85 0.03532632651826617 1105 86-87 0.31633191221722257 1105 88-89 0.3997184591043421 1105 90-91 0.30429206577037604 1105 92-93 0.3277222150412129 1105 94-95 0.17494683564516578 1105 96-97 0.16359898637725934 1105 98-99 0.31468218727459174 1105 100-101 0.3150102071390606 1106 1 0.18049867927334873 1106 2 0.1725860946604243 1106 3 0.2178959229341757 1106 4 0.16058846076415279 1106 5 0.2491995585550697 1106 6 0.17709247690606134 1106 7 0.08263203214201553 1106 8 0.2268083782661634 1106 9 0.25752099399990414 1106 10-11 0.24549725092755637 1106 12-13 0.25819407484927837 1106 14-15 0.2894337855016573 1106 16-17 0.2785750830682403 1106 18-19 0.27781122548017834 1106 20-21 0.4604047708144634 1106 22-23 0.2578157491866975 1106 24-25 0.3692720404866563 1106 26-27 0.33855163858456194 1106 28-29 0.14596853556065525 1106 30-31 0.19079447201497857 1106 32-33 0.04607799139868263 1106 34-35 -0.11494007210802692 1106 36-37 0.07004492467082457 1106 38-39 0.01777710068555649 1106 40-41 0.0838256551109211 1106 42-43 0.22172073073565457 1106 44-45 0.2995067547029251 1106 46-47 0.390773378221823 1106 48-49 0.4827368545785973 1106 50-51 0.3204034310543946 1106 52-53 0.26351040135393333 1106 54-55 0.3938344751004408 1106 56-57 0.3603942842413943 1106 58-59 0.24591914461878162 1106 60-61 0.5446601737617911 1106 62-63 0.4180962596857256 1106 64-65 0.4407392754262105 1106 66-67 0.3819946797259206 1106 68-69 0.3384371685420078 1106 70-71 0.6089837588326574 1106 72-73 0.4640914081087928 1106 74-75 0.43846188140637565 1106 76-77 0.2388065917782427 1106 78-79 0.48500247472463087 1106 80-81 0.6926135656524721 1106 82-83 0.5836936538243336 1106 84-85 0.3641049441726878 1106 86-87 0.28661044241732725 1106 88-89 0.35118249174134775 1106 90-91 0.4405908097372091 1106 92-93 0.28635750215138955 1106 94-95 0.375702960993209 1106 96-97 0.30351999385105444 1106 98-99 0.1597060363048044 1106 100-101 0.41181150619030404 1107 1 0.2356581236667381 1107 2 0.1580324649945979 1107 3 0.05819152322376908 1107 4 0.05710594506850697 1107 5 0.1628034927196751 1107 6 0.18950105442493026 1107 7 0.24603479762303948 1107 8 0.1441017124661883 1107 9 0.20825427906769534 1107 10-11 0.06998626005691477 1107 12-13 0.13865616884157106 1107 14-15 0.1555966525644621 1107 16-17 0.1853258333882053 1107 18-19 0.10030311709340367 1107 20-21 0.002062938711304696 1107 22-23 0.2820366828335281 1107 24-25 0.14075114023719237 1107 26-27 0.1580960447502946 1107 28-29 0.04260257442263082 1107 30-31 0.06969428664840649 1107 32-33 0.30644742451766405 1107 34-35 0.378204502613535 1107 36-37 0.3287657832296276 1107 38-39 0.24361276324218295 1107 40-41 0.29435344552789644 1107 42-43 0.22196280558608805 1107 44-45 0.16865745302162338 1107 46-47 0.3710980723221411 1107 48-49 0.3808973098586321 1107 50-51 0.50028521783576 1107 52-53 0.31732498546709564 1107 54-55 0.26978344213566885 1107 56-57 0.24843354519583727 1107 58-59 0.28521035842242526 1107 60-61 0.3741262866448878 1107 62-63 0.3425184740878393 1107 64-65 -0.0640966179980964 1107 66-67 0.2998382859239328 1107 68-69 0.2636595748108448 1107 70-71 0.3104281387736165 1107 72-73 0.30654662986505343 1107 74-75 0.2525618448710105 1107 76-77 0.14372945283399474 1107 78-79 0.26977663906207994 1107 80-81 0.3606549783833728 1107 82-83 0.40241209163463765 1107 84-85 0.3335519600398058 1107 86-87 0.13077924031397004 1107 88-89 0.1254129514818345 1107 90-91 0.23219462286439096 1107 92-93 0.12926213138461407 1107 94-95 0.1580418075065424 1107 96-97 0.13038828495950838 1107 98-99 0.2751891885676443 1107 100-101 0.21158870767054339 1108 1 0.2127337898243411 1108 2 0.1868839950916552 1108 3 0.10200812855303099 1108 4 0.14373146977455065 1108 5 0.1405196549174761 1108 6 0.22053486093253838 1108 7 0.15541608262082462 1108 8 0.29173083396432276 1108 9 0.3358068748500216 1108 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-0.6862147775123013 2316 100-101 -1.0238471754598208 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 14.0 14 63.0 15 213.0 16 669.0 17 1465.0 18 2536.0 19 3272.0 20 4347.0 21 6206.0 22 6994.0 23 5523.0 24 3969.0 25 3469.0 26 3813.0 27 4434.0 28 5395.0 29 6582.0 30 7921.0 31 9801.0 32 12223.0 33 15899.0 34 21499.0 35 33081.0 36 54288.0 37 124062.0 38 280024.0 39 101632.0 40 53.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.282794852611936 25.301687335389545 15.152088240938255 21.263429571060264 2 13.427379617003945 24.13956546335795 42.765622372062445 19.66743254757566 3 14.401894788636271 32.00541527033958 33.86323106497073 19.729458876053414 4 8.910904852393111 21.154554565964716 46.11907247521118 23.81546810643098 5 8.595327813368286 42.881288319255425 38.82123680761249 9.70214705976379 6 22.291225646293206 46.500863855533886 20.319438903938984 10.888471594233922 7 22.818017677417025 36.207847950306416 27.15911760249858 13.815016769777982 8 22.447734307782763 38.52990274501737 23.276980022211443 15.74538292498843 9 25.692995611919677 16.605902572663247 25.018034634838603 32.68306718057847 10-11 20.332573955902365 29.330571034222025 33.704404342767866 16.632450667107747 12-13 21.363849279101093 29.28970642811374 31.528433565131092 17.81801072765408 14-15 20.498464797365763 30.43044051767394 29.066619037624626 20.00447564733567 16-17 17.512429651024604 33.3100747933488 30.44156013838368 18.735935417242917 18-19 17.146038148638752 32.33203465429794 33.75763952691574 16.76428767014757 20-21 17.99293626094414 31.686332179209366 33.54462929269483 16.776102267151668 22-23 18.66484208799816 30.727215990870622 32.96605463589161 17.64188728523961 24-25 18.294903460842953 31.482148143926807 32.70183154052616 17.521116854704086 26-27 17.645309118505967 31.334535179005048 33.67361689292778 17.346538809561206 28-29 17.864762152366243 29.460559856577508 34.92885529635111 17.745822694705144 30-31 18.228094651209833 31.19283018684306 33.192083620941936 17.386991541005166 32-33 17.45930036590578 31.983084241727163 33.25141862331859 17.30619676904847 34-35 17.69409645436994 31.218821625994337 33.712188077264685 17.374893842371037 36-37 18.81662216501795 29.944096106881794 32.4639411549672 18.77534057313305 38-39 17.384137027086005 32.21333270322149 33.15342713660037 17.24910313309213 40-41 16.778673679440796 31.037154822649 34.8835706214061 17.300600876504102 42-43 17.407905216353072 31.050150879353506 32.476728718783406 19.065215185510024 44-45 17.894666612921835 31.45511356607626 32.75659567252161 17.893624148480296 46-47 17.815091827217774 30.63914189886983 33.09942747852871 18.446338795383692 48-49 17.109504863352686 31.219588837960778 32.86077484112677 18.810131457559763 50-51 17.700749966984773 30.596498300584546 31.540177795694813 20.16257393673587 52-53 17.645656606653148 31.380890097838765 32.353995905199675 18.619457390308416 54-55 18.312848579619494 30.823525323603512 31.819164277647467 19.04446181912953 56-57 18.22672628636637 31.50758427630034 31.04702348602889 19.218665951304402 58-59 17.711123373581728 30.665898486202636 31.818516670396374 19.804461469819266 60-61 19.070566502976583 32.67153057409212 30.081284427388184 18.176618495543117 62-63 17.854844471255085 32.09240682800309 31.982392080606132 18.070356620135687 64-65 17.782983922418406 30.59084104641191 31.857435342880454 19.768739688289234 66-67 18.30220071193372 31.146057607974992 30.809550086246556 19.742191593844733 68-69 18.00349990061839 31.29784043066291 31.04417408322202 19.65448558549668 70-71 18.49582110754202 30.203391762306985 32.77744496135237 18.523342168798624 72-73 18.62390523859231 30.657558770670335 31.024436756462237 19.69409923427512 74-75 17.76713846290703 31.505916333193873 30.63983687516419 20.087108328734907 76-77 17.55093133773207 31.362751216556 32.1125611405395 18.973756305172433 78-79 17.221097900328864 31.21749234827909 31.295816503253892 20.26559324813815 80-81 17.95004392842447 31.509052591777042 30.348772228338838 20.192131251459646 82-83 18.457168354652083 31.115877441972316 30.881862334896677 19.545091868478924 84-85 17.254940933964743 30.936730748114183 30.90941817974589 20.898910138175186 86-87 17.08995356168401 31.625452255823554 31.49153032390065 19.793063858591783 88-89 18.435983648597745 30.31535244332816 31.039378746790945 20.209285161283148 90-91 17.52368826699321 30.285190472152994 32.33474506184594 19.85637619900785 92-93 18.415412350284733 31.63240201876714 30.829634901153526 19.122550729794607 94-95 17.70035124101917 29.790158857681366 31.752702415320616 20.756787485978855 96-97 18.69979664993627 29.70015942756193 31.079617874234312 20.520426048267492 98-99 17.350430676809616 30.58778315071673 31.11471417709942 20.947071995374237 100-101 17.273844289171297 30.11923013306016 31.692239477711414 20.91468610005713 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 367.0 1 262.0 2 281.5 3 526.0 4 416.0 5 238.5 6 473.0 7 959.0 8 1360.5 9 1529.5 10 1261.0 11 862.5 12 834.0 13 868.0 14 902.0 15 907.0 16 888.0 17 925.5 18 959.0 19 1003.0 20 1122.5 21 1299.5 22 1488.0 23 1732.0 24 1960.0 25 2584.5 26 4366.0 27 6313.5 28 6573.0 29 8181.5 30 13577.0 31 18745.0 32 28184.0 33 39244.0 34 48048.0 35 54241.0 36 59557.5 37 62171.5 38 53277.5 39 44907.5 40 38564.5 41 32418.5 42 27090.0 43 24145.0 44 23807.5 45 21711.0 46 17692.0 47 12503.0 48 10679.0 49 9008.0 50 7067.0 51 5001.5 52 2878.5 53 2060.5 54 1652.0 55 1706.0 56 1651.0 57 1341.5 58 1057.5 59 844.0 60 704.0 61 613.5 62 510.0 63 391.0 64 331.0 65 260.5 66 171.5 67 89.0 68 48.0 69 38.5 70 32.5 71 28.5 72 24.0 73 29.0 74 30.0 75 25.5 76 17.0 77 5.0 78 3.0 79 1.5 80 0.0 81 0.5 82 1.5 83 1.0 84 0.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045451449651052404 2 0.0022239241419475184 3 2.779905177434398E-4 4 0.010007658638763831 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0018764359947682184 24-25 0.0 26-27 0.0 28-29 0.02439366793198684 30-31 0.021613762754552443 32-33 2.0849288830757985E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03384534553526379 50-51 0.01167560174522447 52-53 0.0 54-55 0.0015984454770247788 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.169857766151597E-4 80-81 0.013482540110556829 82-83 0.007088758202457715 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 719449.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.062323523179376 #Duplication Level Percentage of deduplicated Percentage of total 1 72.31283412003722 10.168864682739676 2 9.5428578450316 2.6838950870508946 3 3.641533087491248 1.536252491899926 4 2.1263967663692345 1.196083170693066 5 1.4225843134793419 1.0002420427573266 6 1.0606895661150268 0.8949455901822161 7 0.8117623993200762 0.7990685838233865 8 0.6778016083358038 0.7625172400759511 9 0.5208475485484942 0.6591894060549421 >10 5.413415499765121 16.741780161511844 >50 1.1366672988426232 11.36732697461287 >100 1.1786550090901082 32.81467075296673 >500 0.11896517903454017 11.262452737693527 >1k 0.03498975853957064 8.11271107793764 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4426 0.6151930157662322 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4037 0.5611238600651332 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 3027 0.4207386486046961 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 2727 0.37904007094318015 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2606 0.362221644619702 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2487 0.34568120881396736 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 2223 0.3089864604718333 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1932 0.2685388401401628 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 1804 0.2507474470045827 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1662 0.23101012024479844 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1592 0.22128045212377803 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1585 0.22030748531167602 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1541 0.21419169392132034 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1461 0.20307207321158274 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1407 0.19556632923250988 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1391 0.19334240509056236 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1357 0.1886165662889239 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1297 0.1802768507566207 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1283 0.1783309171324166 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1268 0.17624598824934082 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 1222 0.1698522063412417 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 1210 0.16818426323478106 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 1204 0.16735029168155074 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 1196 0.16623832961057697 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 1121 0.155813685195198 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1106 0.1537287563121222 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1099 0.15275578950002014 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 1088 0.15122684165243125 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1081 0.1502538748403292 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 1081 0.1502538748403292 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1059 0.14719597914515137 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1050 0.14594502181530586 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1041 0.1446940644854604 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1036 0.1439990881911018 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 1001 0.1391342541305916 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 989 0.13746631102413098 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 985 0.1369103299886441 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 964 0.13399142955233798 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 964 0.13399142955233798 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 949 0.13190650066926216 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 949 0.13190650066926216 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 945 0.13135051963377528 No Hit GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG 939 0.13051654808054497 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 936 0.13009956230392983 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 929 0.12912659549182776 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 929 0.12912659549182776 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 920 0.1278756381619823 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 901 0.12523472824341964 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 898 0.12481774246680445 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 892 0.12398377091357414 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 877 0.12189884203049833 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 867 0.12050888944178115 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 861 0.11967491788855082 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 847 0.11772898426434673 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 843 0.11717300322885986 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 837 0.11633903167562955 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 834 0.11592204589901439 No Hit TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA 819 0.1138371170159386 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 802 0.11147419761511936 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 797 0.11077922132076073 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 775 0.10772132562558291 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 762 0.10591438726025054 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 756 0.10508041570702023 No Hit CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC 738 0.10257850104732928 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 728 0.10118854845861207 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 726 0.10091055794086863 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 724 0.1006325674231252 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.389952588717199E-4 0.0 9 0.0 0.0 0.0 1.389952588717199E-4 0.0 10-11 0.0 0.0 0.0 8.339715532303194E-4 0.0 12-13 0.0 0.0 0.0 0.0026409099185626776 0.0 14-15 0.0 0.0 0.0 0.004378350654459176 0.0 16-17 0.0 0.0 0.0 0.010146653897635551 0.0 18-19 0.0 0.0 0.0 0.016540435805734666 0.0 20-21 0.0 0.0 0.0 0.030231468804599072 0.0 22-23 0.0 0.0 0.0 0.05629307984304655 0.0 24-25 0.0 0.0 0.0 0.08374464347021122 0.0 26-27 0.0 0.0 0.0 0.09653220728640946 0.0 28-29 0.0 0.0 0.0 0.10758233036671119 0.0 30-31 0.0 0.0 0.0 0.1257907092789065 0.0 32-33 0.0 0.0 0.0 0.1500453819520216 0.0 34-35 0.0 0.0 0.0 0.19084049043087142 0.0 36-37 0.0 0.0 0.0 0.2477590489388407 0.0 38-39 0.0 0.0 0.0 0.3333106307743843 0.0 40-41 0.0 0.0 0.0 0.4294258522841786 0.0 42-43 0.0 0.0 0.0 0.5190082966270021 0.0 44-45 0.0 0.0 0.0 0.6131775845125922 0.0 46-47 0.0 0.0 0.0 0.7261807299753005 0.0 48-49 0.0 0.0 0.0 0.8491220364473369 0.0 50-51 0.0 0.0 0.0 0.9817235134109575 0.0 52-53 0.0 0.0 0.0 1.1029968767765332 0.0 54-55 0.0 0.0 0.0 1.2319844770094892 0.0 56-57 0.0 0.0 0.0 1.3752885889062325 0.0 58-59 0.0 0.0 0.0 1.5342991650554798 0.0 60-61 0.0 0.0 0.0 1.7105451533048206 0.0 62-63 0.0 0.0 0.0 1.8854706865948803 0.0 64-65 0.0 0.0 0.0 2.0674849780873976 0.0 66-67 0.0 0.0 0.0 2.270487553669544 0.0 68-69 0.0 0.0 0.0 2.5046250672389565 0.0 70-71 0.0 0.0 0.0 2.7765692912214766 0.0 72-73 0.0 0.0 0.0 3.0482355246862527 0.0 74-75 0.0 0.0 0.0 3.313716469131238 0.0 76-77 0.0 0.0 0.0 3.60442505306144 0.0 78-79 0.0 0.0 0.0 3.9371102051709017 0.0 80-81 0.0 0.0 0.0 4.324976475052436 0.0 82-83 0.0 0.0 0.0 4.712703749675098 0.0 84-85 0.0 0.0 0.0 5.079859725984747 0.0 86-87 0.0 0.0 0.0 5.465988555130385 0.0 88-89 0.0 0.0 0.0 5.8687968153406285 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACTGCA 25 0.005023263 56.99212 9 GACATAT 155 0.0 55.18435 1 GTACTAA 50 2.8282426E-5 47.49343 9 ATGGATG 45 9.629222E-4 42.216385 5 GTTATAT 70 4.295587E-6 40.731304 1 CATGGGT 385 0.0 40.708656 4 CATATAG 215 0.0 39.761944 3 CATTCCG 205 0.0 39.384796 9 TGGGTAT 185 0.0 38.508186 6 GGACAAT 260 0.0 38.36008 6 TACCTGG 150 0.0 37.997387 2 GACGTGC 25 0.0016170494 37.994743 88-89 ACGTGCA 25 0.0016170494 37.994743 90-91 ACATATA 230 0.0 37.171356 2 GGCATTC 230 0.0 37.16877 7 GTGTAGC 65 1.32443E-4 36.553734 1 GGTATCA 2510 0.0 36.53917 1 GGTATGG 130 2.055458E-10 36.53341 8 GACAATG 280 0.0 35.620075 7 ACCTGGG 160 1.8189894E-12 35.620075 3 >>END_MODULE