FastQCFastQC Report
Fri 10 Feb 2017
SRR2031669_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031669_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399512
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG35300.030.1957051
GGACCGT1955.347829E-1026.795866
TACATGG37650.026.3905562
ACATGGG39100.024.1795463
GTATAGA5700.022.5403561
GTATAGG4250.022.3930341
GTCTAGA7000.021.0734461
CATGGGG24000.020.9829444
CCGTAAA1301.461558E-820.13255962-63
GGTATCA32500.020.058991
TACACTG12750.019.7486535
TATACTG8450.019.6780995
ACTATAC5400.019.355343
GTGTACG2001.2653505E-519.034081
TAGCACG1250.00602550919.0034224
TAGACTG5950.018.3646535
GTATCAA38350.017.7436331
CCGCGTG1350.00940132317.5926139
GAGTACA26700.017.4657291
TACCTGG12600.017.3561952
Produced by FastQC (version 0.11.5)