FastQCFastQC Report
Fri 10 Feb 2017
SRR2031669_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031669_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399512
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG804.495858E-429.6855585
GTATAGG4650.026.5744921
GTACATG37300.023.062921
TACATGG38550.021.5638052
ACATGGG36900.021.4981863
TATACTG7600.021.248615
TATGTCG1150.003725088420.6508245
GTATAGA6200.019.9308681
GTATCAA41150.019.865671
TCAACGC40650.019.3974114
GGTATCA31950.019.189491
ACGCTCG757.7282416E-418.99875668-69
GGACCGT1759.94373E-518.9987566
CAACGCA41050.018.9756165
GTATATA8350.018.7833061
ATCAACG42000.018.7745913
TATCAAC43350.018.2994962
AACGCAG42950.018.2467696
GTGTAAC5250.018.1056961
TATAAGG5250.018.0959932
Produced by FastQC (version 0.11.5)