##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031666_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 847655 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52370480914995 34.0 31.0 34.0 31.0 34.0 2 32.730522441323416 34.0 31.0 34.0 31.0 34.0 3 32.81439500740277 34.0 31.0 34.0 31.0 34.0 4 36.271215293958036 37.0 37.0 37.0 35.0 37.0 5 36.02627366086438 37.0 35.0 37.0 35.0 37.0 6 36.09428246161469 37.0 37.0 37.0 35.0 37.0 7 36.042680099804755 37.0 37.0 37.0 35.0 37.0 8 36.1428022013673 37.0 37.0 37.0 35.0 37.0 9 38.01640054031416 39.0 39.0 39.0 35.0 39.0 10-11 37.992749408662725 39.0 38.5 39.0 35.0 39.0 12-13 38.03317505353003 39.0 39.0 39.0 35.0 39.0 14-15 39.43230205685096 41.0 39.0 41.0 36.5 41.0 16-17 39.39035987518507 41.0 39.0 41.0 36.0 41.0 18-19 39.49484696014298 41.0 39.5 41.0 37.0 41.0 20-21 39.545090868336764 41.0 40.0 41.0 37.0 41.0 22-23 39.46544702738732 41.0 39.0 41.0 36.5 41.0 24-25 39.40029021240953 41.0 39.0 41.0 36.0 41.0 26-27 39.27910470651385 41.0 39.0 41.0 36.0 41.0 28-29 39.192562422211864 41.0 39.0 41.0 36.0 41.0 30-31 39.04434528198382 40.0 39.0 41.0 35.0 41.0 32-33 38.85015837811373 40.0 38.0 41.0 35.0 41.0 34-35 38.75417829187582 40.0 38.0 41.0 35.0 41.0 36-37 38.595623809214835 40.0 38.0 41.0 35.0 41.0 38-39 38.44698019831181 40.0 38.0 41.0 35.0 41.0 40-41 38.36479286974064 40.0 38.0 41.0 34.0 41.0 42-43 38.21646955424082 40.0 38.0 41.0 34.0 41.0 44-45 38.10083052657036 40.0 38.0 41.0 34.0 41.0 46-47 37.9852221717562 40.0 38.0 41.0 33.0 41.0 48-49 37.976661495537684 40.0 38.0 41.0 33.0 41.0 50-51 37.894672950669786 40.0 38.0 41.0 33.0 41.0 52-53 37.84609245506722 40.0 38.0 41.0 33.0 41.0 54-55 37.757850776554136 40.0 38.0 41.0 33.0 41.0 56-57 37.688956002147094 40.0 38.0 41.0 33.0 41.0 58-59 37.54083441966366 40.0 38.0 41.0 32.5 41.0 60-61 37.330172652789166 40.0 37.5 41.0 32.0 41.0 62-63 37.13664698491721 40.0 37.0 41.0 31.5 41.0 64-65 36.8811680459621 39.5 36.5 41.0 31.0 41.0 66-67 36.62124921105874 39.0 36.0 41.0 31.0 41.0 68-69 36.31055146256437 39.0 36.0 41.0 31.0 41.0 70-71 35.951920297762655 38.0 35.0 40.0 30.0 41.0 72-73 35.568516672466984 37.0 35.0 39.5 30.0 41.0 74-75 35.141765222879584 37.0 35.0 39.0 29.0 41.0 76-77 34.45488612702101 36.0 34.0 39.0 29.0 40.0 78-79 34.34104205130625 36.0 35.0 37.5 29.0 39.5 80-81 34.019217134329416 35.5 34.5 37.0 29.0 39.0 82-83 33.69511888681126 35.0 34.0 37.0 29.0 39.0 84-85 33.337268700119736 35.0 34.0 36.0 29.0 37.5 86-87 33.08174080256708 35.0 34.0 36.0 28.0 37.0 88-89 32.818342368062474 35.0 34.0 36.0 27.5 37.0 90-91 32.65888185641564 35.0 34.0 35.0 27.0 36.0 92-93 32.53353133055312 35.0 34.0 35.0 26.5 36.0 94-95 32.40483569376692 35.0 34.0 35.0 26.0 36.0 96-97 32.303440668668266 35.0 34.0 35.0 26.0 36.0 98-99 32.22878234659148 35.0 34.0 35.0 25.5 35.5 100-101 31.826505476874434 34.5 33.0 35.0 24.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19260531893920785 1101 2 0.20592242952179163 1101 3 0.28014979243432236 1101 4 0.1972499028743897 1101 5 0.2970895019265143 1101 6 0.289997050753243 1101 7 0.2899579888343098 1101 8 0.29737621215170407 1101 9 0.2519891188433263 1101 10-11 0.14080122675498785 1101 12-13 0.1429238881233701 1101 14-15 0.17353678809914896 1101 16-17 0.18220655961718535 1101 18-19 0.1868997268520971 1101 20-21 0.09723531943394192 1101 22-23 0.2060495299391647 1101 24-25 0.1707533027241368 1101 26-27 0.2321700744983346 1101 28-29 0.34949828750206535 1101 30-31 0.32907857140160246 1101 32-33 0.2380993554253621 1101 34-35 0.3750552455969185 1101 36-37 0.23659915759922967 1101 38-39 0.24803786803100536 1101 40-41 0.29759356901080736 1101 42-43 0.2145413161080043 1101 44-45 0.17643276955428888 1101 46-47 0.2958222631083487 1101 48-49 0.26735525441324626 1101 50-51 0.3916711607081709 1101 52-53 0.16864968811380976 1101 54-55 0.2542930184619294 1101 56-57 0.34160458139010785 1101 58-59 0.26696447669053214 1101 60-61 0.1885150802857467 1101 62-63 0.1611487664752076 1101 64-65 0.1706298489633582 1101 66-67 0.21869492970331095 1101 68-69 0.21483233515682798 1101 70-71 0.15541740849697305 1101 72-73 0.41735514698253695 1101 74-75 0.24669054271325308 1101 76-77 0.39008739616943444 1101 78-79 0.48696261428766263 1101 80-81 0.4007271524173177 1101 82-83 0.4640671980064752 1101 84-85 0.3948308628775479 1101 86-87 0.1742816721116398 1101 88-89 0.35591793783396497 1101 90-91 0.5565876597750048 1101 92-93 0.3872804502980429 1101 94-95 0.3513703366183307 1101 96-97 0.3114197869138451 1101 98-99 0.3588385276170598 1101 100-101 0.34434289742237567 1102 1 0.21657440939091543 1102 2 0.167072636251973 1102 3 0.22153216478945836 1102 4 0.25411521542074667 1102 5 0.16107544900444992 1102 6 0.10065671675983623 1102 7 0.1787322067965107 1102 8 0.15666638478161587 1102 9 0.20106662707275547 1102 10-11 0.3290880216094152 1102 12-13 0.26659433248037345 1102 14-15 0.3561839234402342 1102 16-17 0.33196607318311777 1102 18-19 0.3539050310991456 1102 20-21 0.24159598243257818 1102 22-23 0.2050830690351404 1102 24-25 0.2606825435954363 1102 26-27 0.26574249671381267 1102 28-29 0.04245613088594524 1102 30-31 0.0033597459472360924 1102 32-33 0.1982433247738271 1102 34-35 0.3038682510000683 1102 36-37 0.23296659764962868 1102 38-39 0.4937393208399783 1102 40-41 0.33055429350172005 1102 42-43 0.48080935796575375 1102 44-45 0.5161002382007496 1102 46-47 0.6466002695112891 1102 48-49 0.5452810293557846 1102 50-51 0.45702176348306267 1102 52-53 0.3020567691868692 1102 54-55 -0.01545922783402176 1102 56-57 0.11394659889605663 1102 58-59 0.2592446148976606 1102 60-61 0.633134397893393 1102 62-63 0.38315956417633856 1102 64-65 0.4818431605378777 1102 66-67 0.4695673246127825 1102 68-69 0.31550982532559146 1102 70-71 0.5458181618849594 1102 72-73 0.512747740817403 1102 74-75 0.5850679634344331 1102 76-77 0.5086288249835178 1102 78-79 0.2111105913850082 1102 80-81 0.259117516619348 1102 82-83 0.1940397414864634 1102 84-85 0.08586115965307073 1102 86-87 0.05180882224527039 1102 88-89 0.17064555496494904 1102 90-91 0.13784885362836263 1102 92-93 0.2769340509293059 1102 94-95 0.3619562548852002 1102 96-97 0.483458428149369 1102 98-99 0.3196350462077291 1102 100-101 0.25456308710242226 1103 1 0.2286821119117306 1103 2 0.2327575314667456 1103 3 0.20890677881075703 1103 4 0.062334810653190686 1103 5 -0.0024455293563789837 1103 6 0.03852252102677056 1103 7 0.2820800961706027 1103 8 0.15836560460218863 1103 9 0.23952746279656623 1103 10-11 0.09116679476053235 1103 12-13 0.18176123971759495 1103 14-15 0.20479805437150844 1103 16-17 0.1969055042185417 1103 18-19 0.19447375205557904 1103 20-21 0.15121454108003007 1103 22-23 0.09983497685094278 1103 24-25 0.08027290372040596 1103 26-27 0.17883257972587785 1103 28-29 0.26058357846287805 1103 30-31 0.09466388193354192 1103 32-33 0.20389521005516542 1103 34-35 0.2333232216748229 1103 36-37 0.37958064331539987 1103 38-39 0.2957193078065359 1103 40-41 0.1728740026245248 1103 42-43 0.0446732254875144 1103 44-45 0.22302440670976154 1103 46-47 0.2699337855564252 1103 48-49 0.2851237753924565 1103 50-51 0.45036496325406716 1103 52-53 0.6841345678749988 1103 54-55 0.4882132795201315 1103 56-57 0.47604790650357387 1103 58-59 0.59581499244117 1103 60-61 0.6847687550218922 1103 62-63 0.5350478907188858 1103 64-65 0.7490317739379577 1103 66-67 0.6955391991750375 1103 68-69 0.5379310004061466 1103 70-71 0.46409421506627524 1103 72-73 0.45171895152674324 1103 74-75 0.4278079097171599 1103 76-77 0.418332649141675 1103 78-79 0.6117305934557464 1103 80-81 0.6759805984803293 1103 82-83 0.4835435571180682 1103 84-85 0.38151322764623075 1103 86-87 0.41757959201011374 1103 88-89 0.45406506736803465 1103 90-91 0.3791898364960815 1103 92-93 0.39044376206193476 1103 94-95 0.5474513734347113 1103 96-97 0.3864019153869407 1103 98-99 0.3152804147678978 1103 100-101 0.3656610023606852 1104 1 0.1474478302943254 1104 2 0.227570815285425 1104 3 0.21469842517305437 1104 4 0.12558362179734672 1104 5 0.19924267394497264 1104 6 0.20226255788269754 1104 7 0.23254863700059047 1104 8 0.17733128601683035 1104 9 0.22135155134042606 1104 10-11 0.13273053927834866 1104 12-13 0.19276480768541404 1104 14-15 0.22621426313963866 1104 16-17 0.1530144588655773 1104 18-19 0.11879866677723072 1104 20-21 0.025254634508129925 1104 22-23 0.08135379970162404 1104 24-25 0.09842360975234499 1104 26-27 0.08216977789092539 1104 28-29 0.00871417616750847 1104 30-31 0.15252993470809884 1104 32-33 0.29343047804484 1104 34-35 0.2822329470477243 1104 36-37 0.07131270405486845 1104 38-39 -0.08117459976236319 1104 40-41 0.011125194280964479 1104 42-43 -0.15295130331108453 1104 44-45 -0.23526621743256015 1104 46-47 0.15578402052699403 1104 48-49 0.30958423928429113 1104 50-51 0.2775761791077187 1104 52-53 0.36283262369661173 1104 54-55 0.11341142216042499 1104 56-57 0.20196972487888587 1104 58-59 0.12088038651396715 1104 60-61 0.3835275279098198 1104 62-63 0.27714599022989717 1104 64-65 0.10051581330323245 1104 66-67 -0.027027497178018223 1104 68-69 -0.060056486501039785 1104 70-71 -0.03810705864782449 1104 72-73 0.060250707560641104 1104 74-75 0.021465664866063605 1104 76-77 0.08696253829381817 1104 78-79 0.10667830620353413 1104 80-81 0.25347047430160075 1104 82-83 0.3441715642652099 1104 84-85 0.2384248350241407 1104 86-87 0.13683597598394925 1104 88-89 0.34256467248305 1104 90-91 0.10583140976265071 1104 92-93 0.09189111353196466 1104 94-95 0.0942186377499965 1104 96-97 0.12913575170851743 1104 98-99 0.06696497728575679 1104 100-101 0.1284779605700912 1105 1 0.11076672127656195 1105 2 0.13444491703627648 1105 3 0.0020018218521968834 1105 4 0.058693195440142176 1105 5 0.19865336972853953 1105 6 0.08073923286998763 1105 7 0.19053388504488566 1105 8 0.2248292058609067 1105 9 0.1730620288175686 1105 10-11 0.23628800171680098 1105 12-13 0.21700719994390028 1105 14-15 0.2631821082388228 1105 16-17 0.13967331505248382 1105 18-19 0.12573574491896267 1105 20-21 0.22690222355023337 1105 22-23 0.13354328475528376 1105 24-25 0.1408763818569696 1105 26-27 0.097268279650919 1105 28-29 0.02442748934135608 1105 30-31 0.20508619566960107 1105 32-33 0.17883367647148418 1105 34-35 0.2554679809195193 1105 36-37 0.36200943755039106 1105 38-39 0.30492872725573505 1105 40-41 0.15463660303468885 1105 42-43 0.20321361039543007 1105 44-45 0.23274423875927397 1105 46-47 0.5737062569980651 1105 48-49 0.5896339167501807 1105 50-51 0.7093127618820887 1105 52-53 0.636278575704381 1105 54-55 0.568502922634508 1105 56-57 0.3428283851001481 1105 58-59 0.5061894721776952 1105 60-61 0.6200645652400425 1105 62-63 0.5787307787928242 1105 64-65 0.5509843541332202 1105 66-67 0.6853630197737104 1105 68-69 0.6983929925364407 1105 70-71 0.6375786012057816 1105 72-73 0.5850181962627161 1105 74-75 0.40557151445803896 1105 76-77 0.37941575017771356 1105 78-79 0.37896412050761796 1105 80-81 0.3978350548313472 1105 82-83 0.3965580704300322 1105 84-85 0.3551431317766358 1105 86-87 0.44449675488579743 1105 88-89 0.5777709377543871 1105 90-91 0.44757596133732136 1105 92-93 0.5234341934293099 1105 94-95 0.43072541826671085 1105 96-97 0.38080295490588867 1105 98-99 0.3252834021019666 1105 100-101 0.3538023857346069 1106 1 0.1419845356744034 1106 2 0.24595064089416496 1106 3 0.11965673976992264 1106 4 0.15434732525984884 1106 5 0.20260231585882593 1106 6 0.11397633706635446 1106 7 0.2627750768655801 1106 8 0.21542445664012178 1106 9 0.18270016329144312 1106 10-11 0.07842063557085766 1106 12-13 0.13792511429151233 1106 14-15 -0.04166807703185782 1106 16-17 0.13953971045107494 1106 18-19 0.12554379400427962 1106 20-21 0.13231608494307778 1106 22-23 0.19621737239651793 1106 24-25 0.09393719562737601 1106 26-27 0.1287499208566132 1106 28-29 0.18169955397215887 1106 30-31 0.09331495380637733 1106 32-33 0.1443915940661995 1106 34-35 0.03199906428372401 1106 36-37 0.13176558378486192 1106 38-39 0.11736731564701586 1106 40-41 0.020811300352619355 1106 42-43 0.1241442087241893 1106 44-45 0.10373715771539338 1106 46-47 0.2602301189489964 1106 48-49 0.5258495579592477 1106 50-51 0.4044879445736953 1106 52-53 0.31988089399789743 1106 54-55 0.35442438004085375 1106 56-57 0.2231871557348697 1106 58-59 0.13178371669440736 1106 60-61 0.1383667044357466 1106 62-63 0.17815543340802265 1106 64-65 0.24532154847041454 1106 66-67 0.30169795805345956 1106 68-69 0.328930607305324 1106 70-71 0.4128861031887112 1106 72-73 0.3875573601685005 1106 74-75 0.4302849833239364 1106 76-77 0.3545399263531337 1106 78-79 0.40124395619086783 1106 80-81 0.3365595875112035 1106 82-83 0.3410445129453876 1106 84-85 0.31423644698032405 1106 86-87 0.36618839208335174 1106 88-89 0.38960999663680695 1106 90-91 0.18434107260966215 1106 92-93 0.3286192358022575 1106 94-95 0.3625997555286986 1106 96-97 0.28620159552135505 1106 98-99 0.4137575529939923 1106 100-101 0.48811729008894034 1107 1 0.05945617917344492 1107 2 0.09497401046548504 1107 3 0.064700342347777 1107 4 0.09866648875708961 1107 5 0.026398536915323234 1107 6 0.05292631711223095 1107 7 0.14052758475507687 1107 8 0.03982651391888936 1107 9 0.04203236508939767 1107 10-11 0.08133441930655039 1107 12-13 0.04804979153946931 1107 14-15 0.07183085153555879 1107 16-17 0.19708613786051643 1107 18-19 0.03924258441539763 1107 20-21 0.059271148884441516 1107 22-23 0.10422402697851396 1107 24-25 0.09870535108046852 1107 26-27 0.07838377460004864 1107 28-29 0.08260125624372705 1107 30-31 0.0030669936953984234 1107 32-33 0.09563139462106562 1107 34-35 0.1652061016003472 1107 36-37 0.12035486689469366 1107 38-39 0.06863676691646958 1107 40-41 0.03584341127513824 1107 42-43 0.08822677554648806 1107 44-45 0.015199322602221343 1107 46-47 0.29109571145842494 1107 48-49 0.22797928063692297 1107 50-51 0.22241572825490863 1107 52-53 0.07624417775843995 1107 54-55 0.3080546692875785 1107 56-57 0.44888599102274895 1107 58-59 0.39408195159538195 1107 60-61 0.38355086742812006 1107 62-63 0.30208259843107044 1107 64-65 0.3467502320908835 1107 66-67 0.4617853715655329 1107 68-69 0.31506208658748847 1107 70-71 0.40745601556684363 1107 72-73 0.4604901043341272 1107 74-75 0.42833765260948553 1107 76-77 0.284008088698009 1107 78-79 0.41095977412586393 1107 80-81 0.3760679626360144 1107 82-83 0.3179754556297496 1107 84-85 0.3496209686662226 1107 86-87 0.11693486885722137 1107 88-89 0.22857696574076414 1107 90-91 0.3826314284342658 1107 92-93 0.3722362383233744 1107 94-95 0.4252605305182371 1107 96-97 0.2799140412885919 1107 98-99 0.40173619165755525 1107 100-101 0.498200937158888 1108 1 -0.047897654207787355 1108 2 -0.010921615978091381 1108 3 -0.0837554636422766 1108 4 -0.1239419530294299 1108 5 -0.03364492038840439 1108 6 0.02517324826327183 1108 7 0.048868862959366766 1108 8 -0.028973787758118874 1108 9 -0.05127007067878964 1108 10-11 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-1.6978465228455661 2316 84-85 -1.7157544448351842 2316 86-87 -1.2693639640181615 2316 88-89 -1.0352708502989536 2316 90-91 -0.8449578707725394 2316 92-93 -0.9610991172464729 2316 94-95 -1.0413505062603576 2316 96-97 -0.9301987222961472 2316 98-99 -1.0297905123432685 2316 100-101 -1.2694556694326486 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 3.0 13 12.0 14 81.0 15 256.0 16 659.0 17 1462.0 18 2534.0 19 3465.0 20 4870.0 21 7055.0 22 8294.0 23 6784.0 24 4663.0 25 3700.0 26 4111.0 27 4967.0 28 6052.0 29 7565.0 30 9111.0 31 11428.0 32 14348.0 33 18552.0 34 25614.0 35 39292.0 36 67578.0 37 154151.0 38 330952.0 39 110058.0 40 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.59322798084629 25.942961412851684 15.142502044848575 21.321308561453453 2 13.1473671171304 24.02727556937161 42.49609211403359 20.3292651994644 3 14.247373034421006 32.78318553272514 33.54411190454562 19.42532952830823 4 9.351248162384994 21.187258573373636 46.04597164114653 23.41552162309484 5 8.978416926697772 42.707823347942266 38.16151618288101 10.152243542478958 6 22.384106741539895 46.41227858031864 20.106883106924396 11.096731571217063 7 22.881124985990763 35.78531360046245 27.553544779420875 13.780016634125914 8 22.688239908925212 37.6305218514608 23.369767181223494 16.3114710583905 9 25.462835705564174 16.68037114156113 24.971716087323262 32.885077065551435 10-11 20.225681438792904 29.35923223481251 33.17045260158909 17.244633724805492 12-13 21.86549952516059 28.794497761471355 31.383345818758812 17.956656894609246 14-15 21.15955194035309 29.823159186225528 28.76836684736125 20.248922026060132 16-17 17.941025535152864 32.89864390583433 30.257593006588767 18.90273755242404 18-19 17.5764904353776 31.60065120833358 33.231267437813734 17.591590918475084 20-21 18.097987978599782 31.266317074753292 33.08480454902053 17.550890397626393 22-23 19.045324156465284 30.508701527832915 32.00084705921215 18.445127256489645 24-25 18.631872636862877 31.484153340686955 32.08221505211436 17.80175897033581 26-27 18.159274704921224 31.03048999887926 32.982227439229405 17.828007856970114 28-29 18.236327990177827 28.905430926504227 34.48013824670003 18.378102836617913 30-31 18.821312516076656 30.64919020840069 32.79508484935729 17.734412426165367 32-33 18.032346905490385 30.92492283117709 33.07798165408194 17.964748609250584 34-35 18.182751237236847 30.839197550890397 33.08450961771003 17.89354159416272 36-37 19.340592575989053 29.4275383263238 31.97609876659726 19.255770331089888 38-39 18.278426954362327 31.360164217753685 32.687178156207416 17.674230671676565 40-41 17.676649108422648 30.295108269284082 34.34675664037846 17.68148598191481 42-43 18.04142015324631 30.326960850817848 32.19959771369248 19.432021282243365 44-45 18.233420436380367 30.790592870920364 32.26253605535271 18.71345063734656 46-47 18.80546920622187 30.418448543334257 31.775309530410368 19.000772720033503 48-49 17.913566332882912 30.936620424733434 31.631252544683164 19.51856069770049 50-51 18.113927709706154 30.382158090035453 30.94754912660535 20.556365073653037 52-53 18.190773368882386 30.863086987040717 31.728002548206526 19.21813709587037 54-55 18.860364207784365 30.862044150402053 31.037175808826305 19.240415832987278 56-57 19.07674702561773 31.126519633577338 30.177548648919668 19.61918469188526 58-59 18.058939073089878 30.253640927028098 31.297166889831356 20.39025311005067 60-61 19.637175501825624 31.6778642254219 30.072140198547757 18.612820074204716 62-63 18.794320802685053 31.061221841433127 31.819431254460834 18.32502610142098 64-65 18.729730845686042 30.525508609044955 31.049247630226922 19.695512915042087 66-67 19.015696244344692 31.188278249995577 30.086296901451654 19.70972860420808 68-69 18.76931062755484 30.880782865670586 30.646666391397442 19.70324011537713 70-71 19.079519379936414 30.38199503335673 31.567913832868328 18.97057175383853 72-73 19.070140564262584 30.859724770101042 30.2799487999245 19.790185865711877 74-75 18.666025682618518 30.951035503831157 30.007255310238246 20.37568350331208 76-77 18.13969126590417 31.386000200553294 31.509104529555067 18.96520400398747 78-79 18.19959452771681 30.76251086086473 30.795307280628226 20.242587330790236 80-81 18.274500966594243 31.739804746193055 30.00135093122816 19.98434335598454 82-83 18.96740185191412 31.372229782700643 29.945334496604865 19.715033868780377 84-85 18.204871085524182 30.675274728515728 30.158791017572007 20.961063168388083 86-87 17.67216615250308 31.441742218237373 31.255463602526973 19.630628026732573 88-89 18.920492417316005 30.00182857412509 30.823802136482414 20.253876872076493 90-91 18.337118285151387 30.520140859193894 31.6778642254219 19.46487663023282 92-93 18.989978234069284 31.201609145230076 30.584022981047713 19.224389639652927 94-95 18.312697972642173 29.762698267573484 31.157546407441707 20.76705735234264 96-97 19.246981378037056 29.757212545198225 30.56974830561962 20.4260577711451 98-99 18.025788793789925 30.142038919135732 30.879603140428596 20.952569146645747 100-101 18.153671010021768 30.259539553238053 31.224318856138407 20.362470580601777 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 245.0 1 166.5 2 151.5 3 293.0 4 246.5 5 165.0 6 301.0 7 590.0 8 898.5 9 1013.0 10 856.0 11 664.0 12 640.5 13 636.5 14 684.5 15 709.5 16 700.5 17 778.5 18 867.0 19 964.0 20 1137.5 21 1356.0 22 1644.5 23 1986.5 24 2231.0 25 2834.0 26 4352.5 27 5937.0 28 6159.5 29 7252.0 30 11412.5 31 15879.0 32 24883.5 33 37059.5 34 47071.0 35 55150.5 36 68938.5 37 79320.0 38 73213.0 39 64367.5 40 53152.0 41 42181.5 42 35398.0 43 31900.5 44 30871.5 45 28650.0 46 23004.0 47 16318.0 48 16751.0 49 14590.0 50 9500.5 51 6215.0 52 2838.0 53 1763.0 54 1251.5 55 1002.0 56 912.5 57 872.5 58 1081.5 59 1111.5 60 1195.0 61 1323.0 62 936.5 63 450.0 64 161.5 65 115.0 66 84.5 67 69.5 68 59.0 69 46.5 70 41.0 71 31.5 72 21.5 73 22.5 74 24.5 75 25.0 76 19.0 77 9.0 78 5.5 79 5.5 80 5.0 81 2.0 82 1.0 83 1.0 84 0.5 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04766089977644207 2 0.0011797252419911404 3 4.718900967964561E-4 4 0.010499554653721148 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0019465466492853813 24-25 0.0 26-27 0.0 28-29 0.02176593071473654 30-31 0.018049796202464448 32-33 5.898626209955701E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03539175725973421 50-51 0.011797252419911402 52-53 0.0 54-55 8.258076693937982E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.7695878629867104E-4 80-81 0.010794485964218933 82-83 0.005367749851059689 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 847655.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.8863738054745 #Duplication Level Percentage of deduplicated Percentage of total 1 73.33889834260778 10.184113568671458 2 9.24600939965323 2.567870854650313 3 3.461712819170895 1.4421191464263001 4 1.9853158554364025 1.1027535236210104 5 1.3266618922733968 0.9211261474793265 6 0.9096425999887546 0.7578982303696538 7 0.7478792872580713 0.7269731940966193 8 0.6135301106579225 0.6815766766008038 9 0.5356614808033704 0.6694556000066644 >10 5.355757276277492 16.484239296975602 >50 1.1123956564150879 11.027714605669729 >100 1.2236352220565965 34.79433066752962 >500 0.10268267597677733 9.82972933888638 >1k 0.04021738142423779 8.810099149016521 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4661 0.5498699352920705 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4184 0.4935970412490931 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 3350 0.39520795606703196 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 2716 0.3204133757247937 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2359 0.27829718458571 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 2171 0.25611835003627653 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 2031 0.2396021966484006 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 1950 0.23004642218827234 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 1944 0.22933858704307766 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1923 0.22686116403489628 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1635 0.19288507706555144 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 1630 0.19229521444455588 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1616 0.19064359910576828 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1614 0.19040765405737003 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1605 0.18934590133957802 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1485 0.1751891984356843 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1480 0.17459933581468876 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1460 0.17223988533070647 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 1454 0.1715320501855118 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1438 0.16964448979832597 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1422 0.16775692941114015 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1415 0.16693112174174635 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 1332 0.15713940223321987 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1316 0.15525184184603405 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 1299 0.1532463089346491 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1293 0.15253847378945443 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 1273 0.15017902330547217 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1256 0.14817349039408723 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1236 0.14581403991010494 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1202 0.14180297408733505 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 1195 0.14097716641794125 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 1184 0.139679468651751 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 1150 0.13566840282898113 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1145 0.13507854020798557 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1103 0.13012369419162276 No Hit CCATTATACTAGGACTTTACTTCACCACCCTCCAAGCTTCAGAATACTTT 1090 0.12859005137703428 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 1089 0.12847207885283518 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 1075 0.12682046351404758 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 1065 0.12564073827205643 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 1062 0.1252868206994591 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1059 0.12493290312686174 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 1058 0.12481493060266263 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 1044 0.12316331526387504 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 1026 0.12103980982829099 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 1017 0.11997805711049896 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1016 0.11986008458629985 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 1016 0.11986008458629985 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 992 0.11702874400552112 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 988 0.11655685390872465 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 987 0.11643888138452554 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 984 0.1160849638119282 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 977 0.1152591561425344 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 973 0.11478726604573794 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 968 0.11419740342474237 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 961 0.11337159575534857 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 958 0.11301767818275123 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 948 0.1118379529407601 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 947 0.11171998041656099 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 918 0.10829877721478667 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 902 0.10641121682760085 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 901 0.10629324430340174 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 896 0.10570338168240617 No Hit TACTAGGACTTTACTTCACCACCCTCCAAGCTTCAGAATACTTTGAAACA 896 0.10570338168240617 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 889 0.10487757401301237 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 886 0.10452365644041503 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 871 0.10275406857742833 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 869 0.10251812352903009 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.9493131049778505E-4 0.0 12-13 0.0 0.0 0.0 0.0011797252419911402 0.0 14-15 0.0 0.0 0.0 0.0017695878629867103 0.0 16-17 0.0 0.0 0.0 0.007550241548743298 0.0 18-19 0.0 0.0 0.0 0.012387115040906973 0.0 20-21 0.0 0.0 0.0 0.02211984828733388 0.0 22-23 0.0 0.0 0.0 0.050197309046723024 0.0 24-25 0.0 0.0 0.0 0.08122408291109001 0.0 26-27 0.0 0.0 0.0 0.09290336280680231 0.0 28-29 0.0 0.0 0.0 0.10352088998472256 0.0 30-31 0.0 0.0 0.0 0.1173236753160189 0.0 32-33 0.0 0.0 0.0 0.13808683957506296 0.0 34-35 0.0 0.0 0.0 0.16840577829423528 0.0 36-37 0.0 0.0 0.0 0.20527219210645842 0.0 38-39 0.0 0.0 0.0 0.26184001745993357 0.0 40-41 0.0 0.0 0.0 0.33333136712459666 0.0 42-43 0.0 0.0 0.0 0.39691855766791917 0.0 44-45 0.0 0.0 0.0 0.4650476903929075 0.0 46-47 0.0 0.0 0.0 0.5528782346591479 0.0 48-49 0.0 0.0 0.0 0.6487898968330276 0.0 50-51 0.0 0.0 0.0 0.7500103225958674 0.0 52-53 0.0 0.0 0.0 0.8448602320519552 0.0 54-55 0.0 0.0 0.0 0.9434262760203149 0.0 56-57 0.0 0.0 0.0 1.0662946599736922 0.0 58-59 0.0 0.0 0.0 1.2052662934802485 0.0 60-61 0.0 0.0 0.0 1.3571559183866078 0.0 62-63 0.0 0.0 0.0 1.4994897688328388 0.0 64-65 0.0 0.0 0.0 1.6590476078121406 0.0 66-67 0.0 0.0 0.0 1.841138198913473 0.0 68-69 0.0 0.0 0.0 2.04263527024556 0.0 70-71 0.0 0.0 0.0 2.2890208870354094 0.0 72-73 0.0 0.0 0.0 2.5257917431030315 0.0 74-75 0.0 0.0 0.0 2.771882428582383 0.0 76-77 0.0 0.0 1.1797252419911402E-4 3.043573151812943 0.0 78-79 0.0 0.0 1.1797252419911402E-4 3.3490630032265485 0.0 80-81 0.0 0.0 1.1797252419911402E-4 3.697848771021229 0.0 82-83 0.0 0.0 1.1797252419911402E-4 4.065628115211967 0.0 84-85 0.0 0.0 1.1797252419911402E-4 4.428275654600043 0.0 86-87 0.0 0.0 1.1797252419911402E-4 4.822362871687184 0.0 88-89 0.0 0.0 1.1797252419911402E-4 5.244173631961116 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTTGC 15 6.602079E-4 95.05513 1 GGGACAT 25 0.005023365 56.99271 7 GCCTATG 25 0.005023365 56.99271 9 GACATAT 245 0.0 54.31722 1 ACGCAGT 45 1.5167769E-5 52.771027 8 CGCAGTG 45 1.5167769E-5 52.771027 9 CTCAATT 60 1.4931338E-6 47.493923 9 TAGGGTC 290 0.0 40.945454 4 GTATGGT 140 0.0 40.737915 1 CGCAGAA 105 1.027729E-9 40.709076 9 AGGGTCT 305 0.0 38.929447 5 TAGATAT 245 0.0 36.832024 7 GTGGTAC 155 1.8189894E-12 36.76949 6 CATATAG 390 0.0 36.535942 3 CTCCTCG 65 1.3288899E-4 36.533787 9 GTCTTCG 80 1.0729986E-5 35.645676 1 GGACAAT 280 0.0 35.620445 6 TGGTACT 160 1.8189894E-12 35.620445 7 CTTCGAT 95 8.7010267E-7 34.99759 3 CATCGGA 35 2.0552857E-4 33.924232 16-17 >>END_MODULE