FastQCFastQC Report
Fri 10 Feb 2017
SRR2031665_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031665_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191328
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.038.0638051
GTATCAA38300.035.8179631
TCAACGC37350.034.8668174
CCGTACG550.002578382434.5502369
ATCAACG37900.034.3576933
CAACGCA38650.033.57115
AACGCAG40250.032.455686
TATCAAC45350.029.172032
ACGCAGA45600.028.3340137
CGCAGAG45200.028.1676148
ACATGGG45450.024.4677813
GTACATG50700.024.4271371
GCAGAGT54050.023.1160569
TACATGG50000.023.0326822
GTATAAG12000.020.6409321
GAGTACA37900.019.7318421
CCTACGC1759.903589E-519.0095833
CATGGGG26800.018.6211664
AGAGTAC50250.018.57698410-11
GTGTAGC11900.018.4127241
Produced by FastQC (version 0.11.5)