##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031665_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2191328 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09995810759503 33.0 31.0 34.0 30.0 34.0 2 32.32559434279122 34.0 31.0 34.0 31.0 34.0 3 32.470833211641526 34.0 31.0 34.0 31.0 34.0 4 35.56056464390543 37.0 35.0 37.0 33.0 37.0 5 35.302774390689116 37.0 35.0 37.0 33.0 37.0 6 35.51766873786124 37.0 35.0 37.0 33.0 37.0 7 35.65751270462478 37.0 36.0 37.0 33.0 37.0 8 35.730557451919566 37.0 36.0 37.0 35.0 37.0 9 37.67136184085632 39.0 38.0 39.0 35.0 39.0 10-11 37.63131078505819 39.0 38.0 39.0 35.0 39.0 12-13 37.66102107945502 39.0 38.0 39.0 35.0 39.0 14-15 39.13413897873801 41.0 39.0 41.0 36.0 41.0 16-17 39.10466050723579 41.0 39.0 41.0 36.0 41.0 18-19 39.037583602272235 41.0 39.0 41.0 36.0 41.0 20-21 39.05067109989924 41.0 39.0 41.0 36.0 41.0 22-23 38.99416541019875 41.0 39.0 41.0 35.5 41.0 24-25 38.940306289154336 41.0 39.0 41.0 35.0 41.0 26-27 38.86238048343287 41.0 39.0 41.0 35.0 41.0 28-29 38.77210189437638 41.0 39.0 41.0 35.0 41.0 30-31 38.66145734458739 40.0 38.5 41.0 34.5 41.0 32-33 38.53405012850655 40.0 38.0 41.0 34.0 41.0 34-35 38.42806553834022 40.0 38.0 41.0 34.0 41.0 36-37 38.315147253172505 40.0 38.0 41.0 34.0 41.0 38-39 38.16960103644913 40.0 38.0 41.0 33.5 41.0 40-41 38.04989440193344 40.0 38.0 41.0 33.0 41.0 42-43 37.91204511602097 40.0 38.0 41.0 33.0 41.0 44-45 37.744029875947376 40.0 38.0 41.0 32.5 41.0 46-47 37.586031849180046 40.0 38.0 41.0 32.0 41.0 48-49 37.71684658800508 40.0 38.0 41.0 32.5 41.0 50-51 37.50419175039063 39.5 37.5 40.5 32.5 41.0 52-53 37.65006699134041 40.0 37.5 41.0 32.5 41.0 54-55 37.947382363571315 40.0 38.0 41.0 33.0 41.0 56-57 37.87771205406037 40.0 38.0 41.0 33.0 41.0 58-59 37.73025284211218 40.0 37.0 41.0 32.5 41.0 60-61 37.5082548116941 40.0 37.0 41.0 32.0 41.0 62-63 37.243500516581726 40.0 36.5 41.0 31.0 41.0 64-65 37.027168000408885 39.5 36.0 41.0 31.0 41.0 66-67 36.858375377853065 39.0 35.5 41.0 31.0 41.0 68-69 36.65249748097957 39.0 35.0 41.0 31.0 41.0 70-71 36.39062842258211 38.5 35.0 41.0 31.0 41.0 72-73 36.06774636202339 37.5 35.0 40.5 30.0 41.0 74-75 35.65834051315002 37.0 35.0 39.5 30.0 41.0 76-77 35.26244519305189 36.5 35.0 39.0 29.0 41.0 78-79 34.87999947976752 36.0 35.0 39.0 29.0 40.5 80-81 34.50139025285124 36.0 34.0 38.5 29.0 39.5 82-83 34.117266105302356 35.0 34.0 37.0 29.0 39.0 84-85 33.781787573562696 35.0 34.0 37.0 28.5 39.0 86-87 33.47108853626659 35.0 34.0 36.5 28.0 37.0 88-89 33.16337467508287 35.0 34.0 36.0 27.0 37.0 90-91 32.91767617627302 35.0 34.0 36.0 27.0 37.0 92-93 32.70207700535931 35.0 34.0 35.0 26.0 36.0 94-95 32.51658149761241 35.0 34.0 35.0 26.0 36.0 96-97 32.3870196520101 35.0 33.0 35.0 25.5 36.0 98-99 32.25908878086713 35.0 33.0 35.0 25.0 36.0 100-101 31.80505063596139 34.5 32.5 35.0 24.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1924159255678788 1101 2 0.1338800865933294 1101 3 0.16274589699398945 1101 4 0.13593604703176254 1101 5 0.21360720323576743 1101 6 0.23955262408225764 1101 7 0.23603967568372042 1101 8 0.336656257588686 1101 9 0.2991451292872114 1101 10-11 0.3474422992983861 1101 12-13 0.2950477058446026 1101 14-15 0.33482536043351274 1101 16-17 0.2534948334193743 1101 18-19 0.33688412799467926 1101 20-21 0.1523608522850779 1101 22-23 0.28065835961673 1101 24-25 0.25864320832148024 1101 26-27 0.10587484967606287 1101 28-29 0.22897428575912926 1101 30-31 0.22684992131461001 1101 32-33 0.2810044986435045 1101 34-35 0.2638868185053411 1101 36-37 0.24164364845164243 1101 38-39 0.24297015238112607 1101 40-41 0.21847876364977026 1101 42-43 0.2596722775699405 1101 44-45 0.27592528450902876 1101 46-47 0.27912343384149096 1101 48-49 0.18297956375798208 1101 50-51 0.007886379228146723 1101 52-53 0.06492781845965112 1101 54-55 0.12503601579739865 1101 56-57 0.2598885591828761 1101 58-59 0.2964802663511321 1101 60-61 0.08143471943554204 1101 62-63 -0.051753472927728694 1101 64-65 0.14730621325057314 1101 66-67 0.29065772741454765 1101 68-69 0.049104978505205565 1101 70-71 0.26790007059986465 1101 72-73 0.30220097689460346 1101 74-75 0.16957685493928665 1101 76-77 0.1538156198193903 1101 78-79 0.13133940373679565 1101 80-81 0.13572291015967863 1101 82-83 0.25962199273312336 1101 84-85 0.0694928925839946 1101 86-87 0.1327916193561478 1101 88-89 0.25467445595845106 1101 90-91 0.12102920794173144 1101 92-93 0.04542225682134671 1101 94-95 -0.025078621718968463 1101 96-97 0.21298066437903884 1101 98-99 0.05332599980661712 1101 100-101 0.11013250307759748 1102 1 0.09310808569681228 1102 2 0.1587948444575602 1102 3 0.18380897316768596 1102 4 0.03139040114594138 1102 5 0.23222637083954822 1102 6 0.278130177888535 1102 7 0.17571350048482515 1102 8 0.2679135114388629 1102 9 0.241808292056092 1102 10-11 0.32186334433389163 1102 12-13 0.36790071653068424 1102 14-15 0.2954504092936503 1102 16-17 0.35359014825901625 1102 18-19 0.4798455630693397 1102 20-21 0.28394374011423906 1102 22-23 0.1817350865592502 1102 24-25 0.3149309809610159 1102 26-27 0.23128512443536664 1102 28-29 0.2243306189341041 1102 30-31 0.4057588821122451 1102 32-33 0.2451979907747628 1102 34-35 0.1337414708918203 1102 36-37 0.16237838454680542 1102 38-39 0.13436415446182082 1102 40-41 0.26712150438739 1102 42-43 0.27995449368506087 1102 44-45 0.06869548854892571 1102 46-47 -0.3831258761031293 1102 48-49 0.10883303748776996 1102 50-51 -0.0317991720043338 1102 52-53 0.21306902874763978 1102 54-55 0.26478921951859036 1102 56-57 0.14492984223487326 1102 58-59 0.1473874110552984 1102 60-61 -0.134724303001029 1102 62-63 -0.4651588887349618 1102 64-65 -0.08789923984070214 1102 66-67 0.008607586396692568 1102 68-69 0.06340851868476705 1102 70-71 0.19674408500834062 1102 72-73 0.18847894744020977 1102 74-75 0.17578164052600442 1102 76-77 0.20889105986125145 1102 78-79 0.2692133716604914 1102 80-81 0.08686578620228147 1102 82-83 -0.07148702699734599 1102 84-85 -0.021204739879578938 1102 86-87 -0.0024345804983170183 1102 88-89 -0.04138967258729309 1102 90-91 0.0415498614948433 1102 92-93 0.042768475197561884 1102 94-95 0.12382240118665777 1102 96-97 0.07205932768613366 1102 98-99 0.059466741037347504 1102 100-101 0.0914321524873678 1103 1 -0.02838913451187608 1103 2 -0.00964494012326611 1103 3 -0.033578073222436444 1103 4 0.08584786054558435 1103 5 0.11232509625933318 1103 6 0.1426578309946862 1103 7 0.17649222150738808 1103 8 0.18852526083522037 1103 9 0.3359065855661214 1103 10-11 0.32691526557899664 1103 12-13 0.3306815625046369 1103 14-15 0.3543336881918435 1103 16-17 0.3505606416796425 1103 18-19 0.4268233386894238 1103 20-21 0.12655015927540347 1103 22-23 0.19974457964563186 1103 24-25 0.3094276191517693 1103 26-27 0.22380175770436495 1103 28-29 0.14451813138499148 1103 30-31 0.28465102241484175 1103 32-33 0.3244553849973357 1103 34-35 0.3142436190011537 1103 36-37 0.36716429625306546 1103 38-39 0.2787719833005582 1103 40-41 0.1546242177739714 1103 42-43 0.18668890991772713 1103 44-45 0.17161124215054713 1103 46-47 0.33014170538636023 1103 48-49 0.07471537500125436 1103 50-51 0.04383330515439354 1103 52-53 -0.06535345154158989 1103 54-55 0.03195930658691282 1103 56-57 -0.04920536449309765 1103 58-59 0.037408063484562604 1103 60-61 0.10962166899403059 1103 62-63 0.16134909852649315 1103 64-65 0.2280922748623695 1103 66-67 0.035772282963343116 1103 68-69 0.06875521555163999 1103 70-71 0.2020408388214534 1103 72-73 0.24913646029845182 1103 74-75 0.2237153931577751 1103 76-77 -0.3084522933321239 1103 78-79 -0.0779841812972748 1103 80-81 0.07632808087075915 1103 82-83 -0.13652488048396094 1103 84-85 -0.10194424304942373 1103 86-87 0.0037565445931235786 1103 88-89 0.07426923253820661 1103 90-91 0.0984520194253804 1103 92-93 -0.0057195337263351576 1103 94-95 -0.10657104405259332 1103 96-97 -0.03455025881328311 1103 98-99 -0.023737549489482035 1103 100-101 -0.057209535956147306 1104 1 -0.04484446767016692 1104 2 0.10793943506608628 1104 3 0.1377745554863381 1104 4 0.20410117799018934 1104 5 0.10540434464184756 1104 6 0.09934947527575844 1104 7 0.02966370528540807 1104 8 0.18634500459586434 1104 9 0.12509013400316604 1104 10-11 0.23677408483128914 1104 12-13 0.1697056177650822 1104 14-15 0.19882521532722564 1104 16-17 0.13290207422326006 1104 18-19 0.2476176402924395 1104 20-21 0.22052308111769747 1104 22-23 0.232205918203384 1104 24-25 0.09027928174526778 1104 26-27 0.15784321650476585 1104 28-29 0.23276518759472253 1104 30-31 0.16439182075594516 1104 32-33 0.08065043929503446 1104 34-35 0.1200567379713533 1104 36-37 0.19102404459181344 1104 38-39 0.1764106225255162 1104 40-41 0.17643566708152747 1104 42-43 0.14465232545501294 1104 44-45 0.16007387335034906 1104 46-47 0.30040689897970907 1104 48-49 0.26449084577205184 1104 50-51 0.12273873357770526 1104 52-53 0.14012842790607039 1104 54-55 0.03721551227857134 1104 56-57 0.021706015469597162 1104 58-59 -0.0014185945644911158 1104 60-61 0.050722973081342104 1104 62-63 0.2890373563547115 1104 64-65 0.3211711192942488 1104 66-67 0.14473231204779324 1104 68-69 0.09554697226563746 1104 70-71 0.1251952893475945 1104 72-73 0.14185417286063995 1104 74-75 -0.011497657140814965 1104 76-77 0.04686430297980593 1104 78-79 0.07984916213482052 1104 80-81 0.08643465651388027 1104 82-83 0.11495755102957617 1104 84-85 0.11954875850577906 1104 86-87 0.11935550240048798 1104 88-89 0.1605280437114729 1104 90-91 0.15465178648051392 1104 92-93 0.1721247163729629 1104 94-95 -0.016859801433106725 1104 96-97 0.036328266497591244 1104 98-99 0.08924488539030762 1104 100-101 0.18335168612918196 1105 1 0.18414709436618892 1105 2 0.2905191904893272 1105 3 0.22918979349442736 1105 4 0.198960229356544 1105 5 0.021572947051012648 1105 6 0.15163844448470343 1105 7 0.2587020650132459 1105 8 0.23827684728765064 1105 9 0.24502294402153524 1105 10-11 0.3217898038882723 1105 12-13 0.34665592462982886 1105 14-15 0.3995678345600311 1105 16-17 0.4189525712853239 1105 18-19 0.4132748014503491 1105 20-21 0.33917261477477467 1105 22-23 0.30375710481245477 1105 24-25 0.2774588057944456 1105 26-27 0.35456058067054386 1105 28-29 0.3213193611514171 1105 30-31 0.2343163300664699 1105 32-33 0.3265719159059941 1105 34-35 0.27938771399080053 1105 36-37 0.2934424917712093 1105 38-39 0.3694420802474596 1105 40-41 0.31853358929016906 1105 42-43 0.2627312671474442 1105 44-45 0.23151904619462016 1105 46-47 0.3973147219066462 1105 48-49 0.23595954818187437 1105 50-51 0.17584247935382535 1105 52-53 0.1842625725736795 1105 54-55 0.273170257080821 1105 56-57 0.26618774410835044 1105 58-59 0.20940056111626149 1105 60-61 0.31269658075198237 1105 62-63 0.27680028290978953 1105 64-65 0.2967569727965582 1105 66-67 0.20947796398015583 1105 68-69 0.14999582487251928 1105 70-71 0.19107022970668197 1105 72-73 0.22045847746394287 1105 74-75 0.32217249756607913 1105 76-77 0.2802369394022648 1105 78-79 0.21453056528124392 1105 80-81 0.3160866150234156 1105 82-83 0.3111332123084054 1105 84-85 0.3056182099400999 1105 86-87 0.2111516579309125 1105 88-89 0.2586179658678347 1105 90-91 0.253370993795599 1105 92-93 0.32250007830343463 1105 94-95 0.20768911718727168 1105 96-97 0.33616094514255224 1105 98-99 0.3120086943040903 1105 100-101 0.1742121492694615 1106 1 0.17415458206058787 1106 2 0.2187281585377363 1106 3 0.16540838507138744 1106 4 0.2649754256937058 1106 5 0.1524490072962692 1106 6 0.07748694755957075 1106 7 0.13088638504467554 1106 8 0.23143211260980223 1106 9 0.273762924627178 1106 10-11 0.19187069553765213 1106 12-13 0.25791594402418383 1106 14-15 0.27388381526387207 1106 16-17 0.2753315959210667 1106 18-19 0.26590715565512113 1106 20-21 0.2211059129200379 1106 22-23 0.3173595450643276 1106 24-25 0.1972479228602495 1106 26-27 0.18013019781614048 1106 28-29 0.2587930621591781 1106 30-31 0.30341621099984906 1106 32-33 0.2977806261813143 1106 34-35 0.1805825536191179 1106 36-37 0.2978311399354965 1106 38-39 0.2470061245844235 1106 40-41 0.29323009216864193 1106 42-43 0.24886835332957702 1106 44-45 0.3671407089537695 1106 46-47 0.5531880202974122 1106 48-49 0.4777090909415236 1106 50-51 0.21144953497143604 1106 52-53 0.20161401548921987 1106 54-55 0.322410317473377 1106 56-57 0.28176619918831847 1106 58-59 0.28078603007915603 1106 60-61 0.5229117599042752 1106 62-63 0.5155213601557946 1106 64-65 0.66316668470445 1106 66-67 0.3816851153420586 1106 68-69 0.4677832725507187 1106 70-71 0.4677420109187054 1106 72-73 0.4699568019388849 1106 74-75 0.34938609325513426 1106 76-77 0.36275280472197124 1106 78-79 0.3321583163951942 1106 80-81 0.41180684530070977 1106 82-83 0.491945197529418 1106 84-85 0.44101217313993146 1106 86-87 0.3001727339494238 1106 88-89 0.444655379790035 1106 90-91 0.33767997780138614 1106 92-93 0.4183204718370206 1106 94-95 0.4145275916616029 1106 96-97 0.4488179719141243 1106 98-99 0.3974900548549911 1106 100-101 0.49928770133348976 1107 1 0.006376652034099095 1107 2 0.018043039326997246 1107 3 0.0689805966362016 1107 4 0.16379839088965298 1107 5 0.06419149382225697 1107 6 0.03770664245314492 1107 7 0.006723143974056711 1107 8 0.12120054231657207 1107 9 0.14291566053655913 1107 10-11 0.16522366895502927 1107 12-13 0.13556096092379732 1107 14-15 0.19746790222833965 1107 16-17 0.12847469448688997 1107 18-19 0.14451887576108646 1107 20-21 0.060789387844671694 1107 22-23 0.14677970583897348 1107 24-25 0.13083840427068338 1107 26-27 0.22664933547942212 1107 28-29 0.2121391843844478 1107 30-31 0.13528133386456176 1107 32-33 0.11298250797540987 1107 34-35 0.11405839174828003 1107 36-37 0.1880311947450295 1107 38-39 0.30843528325803504 1107 40-41 0.32616948195579454 1107 42-43 0.196757729414351 1107 44-45 0.02265911582323099 1107 46-47 0.30879069682979576 1107 48-49 0.31900602160745706 1107 50-51 0.2042569251239854 1107 52-53 0.3346474949813185 1107 54-55 0.22080919387786935 1107 56-57 0.3120525790184132 1107 58-59 0.31332713723180206 1107 60-61 0.33898237285759336 1107 62-63 0.37897801494705874 1107 64-65 0.34939464670316056 1107 66-67 0.25255042090523006 1107 68-69 0.3507840946937648 1107 70-71 0.3693547815407996 1107 72-73 0.25748260809582035 1107 74-75 0.3488348006635533 1107 76-77 0.29257992630778773 1107 78-79 0.37342715717348796 1107 80-81 0.3349636462507348 1107 82-83 0.3595586989453281 1107 84-85 0.35113115550952756 1107 86-87 0.2863180248967083 1107 88-89 0.22911710446346234 1107 90-91 0.35264183949865924 1107 92-93 0.3997126340732464 1107 94-95 0.40364812696807917 1107 96-97 0.3935489484373704 1107 98-99 0.2453195971953548 1107 100-101 0.31034278491803136 1108 1 0.04565004473305834 1108 2 0.0890201892588749 1108 3 0.029018231128354444 1108 4 0.016572542494749598 1108 5 0.08620559963397767 1108 6 0.2169386449811057 1108 7 0.05924631262735147 1108 8 0.128506632732595 1108 9 0.008705181933564177 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-1.5921023066734037 2316 72-73 -1.4287095187668797 2316 74-75 -1.5558906754700033 2316 76-77 -1.40066355922891 2316 78-79 -1.564358722179449 2316 80-81 -1.4674259863893084 2316 82-83 -1.5537571031106125 2316 84-85 -1.9974015961041474 2316 86-87 -1.6261142406224494 2316 88-89 -1.3480673325434722 2316 90-91 -1.5193170815309038 2316 92-93 -1.6634776599740881 2316 94-95 -1.7371987532614241 2316 96-97 -1.4295923377208872 2316 98-99 -1.0906832715591257 2316 100-101 -1.71506362196698 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 3.0 7 79.0 8 353.0 9 472.0 10 510.0 11 664.0 12 977.0 13 1354.0 14 1886.0 15 2432.0 16 3173.0 17 4095.0 18 5265.0 19 6777.0 20 8207.0 21 9792.0 22 10770.0 23 11164.0 24 12038.0 25 12625.0 26 14327.0 27 16651.0 28 19557.0 29 23697.0 30 28425.0 31 35198.0 32 44829.0 33 58187.0 34 81043.0 35 120049.0 36 202020.0 37 390933.0 38 677281.0 39 386118.0 40 376.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.89966264450396 21.49176417943021 14.251733772503739 22.356839403562084 2 13.815548124919566 20.817897156476462 43.86038994198639 21.506164776617588 3 17.69838428480701 26.3581915804673 31.623017163058893 24.320406971666802 4 11.143949310260611 17.339712542271176 41.87510690708985 29.641231240378364 5 11.518524838320241 39.27629866461234 37.53234853201595 11.672827965051463 6 28.774196557248725 40.429100246537864 18.796088680473762 12.00061451573965 7 25.414072900969963 33.733660190481764 24.10108188375053 16.751185024797742 8 23.11988884677616 39.58727187689022 21.132462620888045 16.160376655445578 9 24.065727776802433 17.448968208684455 22.248983204588015 36.23632080992509 10-11 22.9177507309146 28.14188110943554 30.65364844585196 18.286719713797893 12-13 23.050998409192893 25.25769177752292 31.137071611922618 20.554238201361567 14-15 20.235351245913975 27.702947697538534 27.8846782552952 24.177022801252292 16-17 20.16062437427379 31.196653415277588 28.506944190703077 20.135778019745544 18-19 20.140133660404697 30.157816422028883 30.164435759670567 19.537614157895856 20-21 20.686330143705522 29.30852455926709 30.241089680359543 19.764055616667843 22-23 20.37914908220038 29.110133216022433 30.07851859694213 20.432199104835057 24-25 20.361397289680045 29.23882230318784 29.96698805473211 20.432792352400007 26-27 20.51358810730297 29.465922034492326 29.662743322770485 20.35774653543422 28-29 20.352567027847954 29.412894829071686 29.92755990887717 20.306978234203186 30-31 20.67935516727756 29.228349201945118 29.758895062719958 20.33340056805736 32-33 20.35393606069014 29.38259356883132 29.80062774719257 20.462842623285972 34-35 20.583408782254413 29.412027774938306 29.65065019933118 20.3539132434761 36-37 20.662767052673082 29.0798547729961 29.720927218563354 20.536450955767464 38-39 20.56953591612027 29.399638027716524 29.676433651192337 20.354392404970866 40-41 20.607321222564583 29.248610888009463 29.746368412213965 20.39769947721199 42-43 20.518402539464653 29.135985119525692 29.79161494764818 20.553997393361467 44-45 20.705552906414702 29.349957193590303 29.428578413642665 20.51591148635233 46-47 20.609639462720867 29.27810919304099 29.408068789873575 20.704182554364564 48-49 20.53739685822362 29.224462447852485 29.60868925790902 20.629451436014875 50-51 20.502744801498476 29.31081326673845 29.449400399459712 20.737041532303362 52-53 20.600142891164563 29.333925782904522 29.465628601248937 20.60030272468197 54-55 20.5779553256993 29.351894266528618 29.410261597995234 20.659888809776845 56-57 20.725723075601714 29.228522910585735 29.31073394189446 20.735020071918093 58-59 20.695689271642483 29.12164810198047 29.39009227682866 20.79257034954839 60-61 20.711977363539653 29.154678823284264 29.39607316636263 20.737270646813457 62-63 20.52114388177403 29.33009019270156 29.35755854177602 20.79120738374839 64-65 20.6766206118496 29.19182683498448 29.325664079938623 20.805888473227295 66-67 20.725108830735685 29.213940475486215 29.171799186364765 20.889151507413324 68-69 20.640680027282226 29.113245447290076 29.280036144837002 20.9660383805907 70-71 20.581306894569614 28.835305743118646 29.496168262567362 21.087219099744384 72-73 20.62624243124472 29.119802681482298 29.27114353680017 20.982811350472815 74-75 20.545237726930093 29.120009468725446 29.245131912095314 21.08962089224915 76-77 20.552945822459236 29.06747730873723 29.310551022727505 21.069025846076027 78-79 20.670283070709527 28.965056634934587 29.13244917959145 21.23221111476443 80-81 20.701661518618607 28.97429233940305 29.103088691817273 21.220957450161062 82-83 20.626855814119192 29.136344473813182 29.181904838899005 21.05489487316862 84-85 20.801898600031425 28.893514136722594 29.144200234607105 21.160387028638876 86-87 20.498340319359226 28.962426163791537 29.314364771705716 21.224868745143517 88-89 20.85632627295354 28.818152626071196 29.06657001295872 21.258951088016538 90-91 20.74408812608708 28.88801168508566 29.1337658130834 21.234134375743853 92-93 20.882967264403142 28.802562295863897 29.033989867558795 21.28048057217416 94-95 20.803905655963035 28.68730168304381 29.12572381119495 21.38306884979821 96-97 20.80896632740121 28.57521297722336 29.14871683849174 21.46710385688369 98-99 20.833250558586826 28.638463883380087 28.93490023074174 21.593385327291347 100-101 20.876125425751923 28.70078252963882 28.8433885903279 21.579703454281354 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 39.0 1 28.5 2 39.0 3 74.0 4 77.0 5 80.0 6 120.0 7 207.0 8 334.0 9 416.5 10 438.0 11 455.5 12 488.0 13 552.5 14 637.0 15 704.0 16 775.5 17 904.0 18 1037.0 19 1192.5 20 1369.5 21 1615.0 22 1931.0 23 2357.5 24 2970.0 25 3867.5 26 5536.5 27 8841.5 28 15648.5 29 26847.0 30 41368.5 31 59001.5 32 77590.5 33 93512.5 34 108555.0 35 119681.5 36 125360.5 37 126171.0 38 116598.0 39 103990.0 40 91802.5 41 79171.5 42 69023.5 43 61974.5 44 58332.0 45 55550.5 46 53871.0 47 53738.0 48 54168.5 49 53861.0 50 52933.0 51 51281.5 52 49330.5 53 47840.5 54 45488.5 55 42181.0 56 38545.5 57 34556.5 58 30680.0 59 26330.0 60 21533.0 61 17032.5 62 13214.5 63 10023.5 64 7391.5 65 5447.5 66 3950.5 67 2923.5 68 2159.5 69 1553.5 70 1099.0 71 772.0 72 578.0 73 445.0 74 329.5 75 226.5 76 152.0 77 104.5 78 70.0 79 57.0 80 40.0 81 28.5 82 21.0 83 17.5 84 16.5 85 15.0 86 14.5 87 12.0 88 9.0 89 7.5 90 5.5 91 3.5 92 4.0 93 5.5 94 6.0 95 3.5 96 1.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46757035003431713 2 0.3593711210736138 3 0.19380941602535084 4 0.22132697615327326 5 0.2451025131792228 6 0.19348997502884097 7 0.18399801398969026 8 0.18605156325296807 9 0.18545831568802115 10-11 0.19814468669227064 12-13 0.18589184275471313 14-15 0.19618240628513853 16-17 0.0854276493523562 18-19 0.035435133398560144 20-21 2.96623782473459E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.13633285386760904 46-47 0.20510393697337873 48-49 0.08515384278391916 50-51 0.06251916645979058 52-53 0.07071054629886535 54-55 0.07972334584325122 56-57 0.11175871435038479 58-59 0.09336803983703033 60-61 0.13690328421851955 62-63 0.23618098249098265 64-65 0.13001248557952072 66-67 0.103293527942873 68-69 0.10756034696768352 70-71 0.08745838140159756 72-73 0.07160041764628572 74-75 0.1401661458257276 76-77 0.21669508170388002 78-79 0.13957289826078068 80-81 0.08328283123293272 82-83 0.0878462740402167 84-85 0.09747513836358591 86-87 0.07299226770250734 88-89 0.09441763168270564 90-91 0.11659596372610583 92-93 0.16439802713240556 94-95 0.13288745454810963 96-97 0.086340337913813 98-99 0.07531962353422217 100-101 0.06292987631244615 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2191328.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.51552273208952 #Duplication Level Percentage of deduplicated Percentage of total 1 68.6593102427818 34.683609473388316 2 15.438112476267607 15.597286434709023 3 6.173444986391352 9.355644016360694 4 3.1031491148523895 6.270287986095576 5 1.8372566573806122 4.6404990220296565 6 1.1410619541596667 3.4584804650445085 7 0.7819945133914621 2.765200313231698 8 0.5653543070205513 2.2847334678385107 9 0.412902317420814 1.8772178761623217 >10 1.8078602380626438 15.07772723156654 >50 0.05900357960746552 1.9736017138148967 >100 0.019732160205632832 1.673522069702759 >500 6.357963561640233E-4 0.1974772672244091 >1k 1.8165610176104394E-4 0.1447126628312 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.12688561456797E-5 0.0 10-11 0.0 0.0 0.0 1.1408607018209963E-4 0.0 12-13 0.0 0.0 0.0 4.3352706669197856E-4 0.0 14-15 0.0 0.0 0.0 7.757852772382774E-4 0.0 16-17 0.0 0.0 0.0 0.0016884738386950746 0.0 18-19 0.0 0.0 0.0 0.002464259115933352 0.0 20-21 0.0 0.0 0.0 0.004312453452883365 0.0 22-23 0.0 0.0 0.0 0.008259831481184012 0.0 24-25 0.0 0.0 0.0 0.01220720950948466 0.0 26-27 0.0 0.0 0.0 0.014260758772762453 0.0 28-29 0.0 0.0 0.0 0.01724981381153346 0.0 30-31 0.0 0.0 0.0 0.02400370916631376 0.0 32-33 0.0 0.0 0.0 0.03974758685144351 0.0 34-35 0.0 0.0 0.0 0.06297551074051899 0.0 36-37 0.0 0.0 0.0 0.0979086654302779 0.0 38-39 0.0 0.0 0.0 0.15454099066867216 0.0 40-41 0.0 0.0 0.0 0.2206880941602535 0.0 42-43 0.0 0.0 0.0 0.2849185516727756 2.2817214036419924E-5 44-45 0.0 0.0 0.0 0.3631131441755867 4.563442807283985E-5 46-47 0.0 0.0 0.0 0.4592648841250602 6.845164210925978E-5 48-49 0.0 0.0 0.0 0.5584284963273412 9.12688561456797E-5 50-51 0.0 0.0 0.0 0.6637071218913828 9.12688561456797E-5 52-53 0.0 0.0 0.0 0.7723626951328144 1.1408607018209963E-4 54-55 0.0 0.0 0.0 0.8924268753924561 1.3690328421851956E-4 56-57 0.0 0.0 0.0 1.0382288730851794 1.3690328421851956E-4 58-59 0.0 0.0 0.0 1.1934771973889806 1.3690328421851956E-4 60-61 0.0 0.0 0.0 1.3551371588370156 1.3690328421851956E-4 62-63 0.0 0.0 0.0 1.5220450795134275 1.3690328421851956E-4 64-65 0.0 0.0 0.0 1.6947942069831625 1.3690328421851956E-4 66-67 0.0 0.0 0.0 1.8993048963915946 1.3690328421851956E-4 68-69 0.0 0.0 0.0 2.135029534601849 1.3690328421851956E-4 70-71 0.0 0.0 0.0 2.395967194322347 1.825377122913594E-4 72-73 0.0 0.0 0.0 2.660373070576381 1.825377122913594E-4 74-75 0.0 0.0 0.0 2.9175230727668335 1.825377122913594E-4 76-77 0.0 0.0 0.0 3.2091498853663167 1.825377122913594E-4 78-79 0.0 0.0 0.0 3.5547165919478965 1.825377122913594E-4 80-81 0.0 0.0 0.0 3.922461630572876 1.825377122913594E-4 82-83 0.0 0.0 0.0 4.312795711093912 1.825377122913594E-4 84-85 0.0 0.0 0.0 4.68718055900349 1.825377122913594E-4 86-87 0.0 0.0 0.0 5.0960878517501715 1.825377122913594E-4 88-89 0.0 0.0 0.0 5.55211269148206 1.825377122913594E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2240 0.0 38.063805 1 GTATCAA 3830 0.0 35.817963 1 TCAACGC 3735 0.0 34.866817 4 CCGTACG 55 0.0025783824 34.550236 9 ATCAACG 3790 0.0 34.357693 3 CAACGCA 3865 0.0 33.5711 5 AACGCAG 4025 0.0 32.45568 6 TATCAAC 4535 0.0 29.17203 2 ACGCAGA 4560 0.0 28.334013 7 CGCAGAG 4520 0.0 28.167614 8 ACATGGG 4545 0.0 24.467781 3 GTACATG 5070 0.0 24.427137 1 GCAGAGT 5405 0.0 23.116056 9 TACATGG 5000 0.0 23.032682 2 GTATAAG 1200 0.0 20.640932 1 GAGTACA 3790 0.0 19.731842 1 CCTACGC 175 9.903589E-5 19.009583 3 CATGGGG 2680 0.0 18.621166 4 AGAGTAC 5025 0.0 18.576984 10-11 GTGTAGC 1190 0.0 18.412724 1 >>END_MODULE