FastQCFastQC Report
Fri 10 Feb 2017
SRR2031665_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031665_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191328
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22900.035.0718651
GTATCAA38950.032.821051
TCAACGC37050.031.666374
ATCAACG38000.030.8754163
CAACGCA37950.030.5371115
AACGCAG38600.030.1459316
TATCAAC44700.027.0982992
ACGCAGA44800.025.5498837
CGCAGAG45450.024.9754838
GTACATG46650.024.3474711
ACATGGG43650.023.8319363
TACATGG50400.021.8658712
GCAGAGT53650.021.4237529
TCGTTAT1908.221767E-620.009861
CATGGGG25300.019.9014
GAGTACA35250.019.2790011
GTATATA21800.019.1837641
TAATACT19750.018.5188144
GTATAGG9200.018.079561
AGAGTAC50950.018.03792810-11
Produced by FastQC (version 0.11.5)