##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031665_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2191328 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52077050993735 34.0 31.0 34.0 31.0 34.0 2 32.73003448137385 34.0 31.0 34.0 31.0 34.0 3 32.84439527081295 34.0 31.0 34.0 31.0 34.0 4 36.27515232772091 37.0 37.0 37.0 35.0 37.0 5 35.97167014705238 37.0 35.0 37.0 35.0 37.0 6 36.02607642488938 37.0 37.0 37.0 35.0 37.0 7 35.99309003490121 37.0 37.0 37.0 35.0 37.0 8 36.11028426597935 37.0 37.0 37.0 35.0 37.0 9 38.017167671841 39.0 39.0 39.0 35.0 39.0 10-11 37.99089684428803 39.0 38.5 39.0 35.0 39.0 12-13 38.004215252121085 39.0 39.0 39.0 35.0 39.0 14-15 39.49724664678223 41.0 39.0 41.0 37.0 41.0 16-17 39.46872147848246 41.0 39.0 41.0 36.0 41.0 18-19 39.47367714919902 41.0 39.5 41.0 36.5 41.0 20-21 39.495526913360294 41.0 39.5 41.0 37.0 41.0 22-23 39.444689704142874 41.0 39.0 41.0 36.5 41.0 24-25 39.38586373194702 41.0 39.0 41.0 36.0 41.0 26-27 39.29388275055126 41.0 39.0 41.0 36.0 41.0 28-29 39.21407406832752 41.0 39.0 41.0 36.0 41.0 30-31 39.1234726156924 41.0 39.0 41.0 36.0 41.0 32-33 39.00006388819931 40.0 39.0 41.0 35.0 41.0 34-35 38.896881251916646 40.0 38.5 41.0 35.0 41.0 36-37 38.77183356393931 40.0 38.0 41.0 35.0 41.0 38-39 38.676942703237486 40.0 38.0 41.0 35.0 41.0 40-41 38.577679379809865 40.0 38.0 41.0 34.5 41.0 42-43 38.46108615414945 40.0 38.0 41.0 34.0 41.0 44-45 38.417128563136146 40.0 38.0 41.0 34.0 41.0 46-47 38.45505168555323 40.0 38.0 41.0 34.0 41.0 48-49 38.576581415470436 40.0 38.0 41.0 35.0 41.0 50-51 38.5240691945706 40.0 38.0 41.0 34.0 41.0 52-53 38.456694068619576 40.0 38.0 41.0 34.0 41.0 54-55 38.380686277910016 40.0 38.0 41.0 34.0 41.0 56-57 38.30632064209466 40.0 38.0 41.0 34.0 41.0 58-59 38.15213514362067 40.0 38.0 41.0 34.0 41.0 60-61 37.92954135574409 40.0 37.0 41.0 33.0 41.0 62-63 37.77610106748054 40.0 37.0 41.0 33.0 41.0 64-65 37.53575183632938 39.5 36.0 41.0 33.0 41.0 66-67 37.30115345580397 39.0 36.0 41.0 32.0 41.0 68-69 37.063091650359965 39.0 35.5 41.0 32.0 41.0 70-71 36.77490612998146 38.5 35.0 41.0 32.0 41.0 72-73 36.43981800077396 37.5 35.0 40.0 31.5 41.0 74-75 36.10121602060485 37.0 35.0 39.5 31.0 41.0 76-77 35.451509997590506 36.0 34.5 39.0 30.5 40.0 78-79 35.393508183165636 36.0 35.0 39.0 31.0 40.0 80-81 35.07439643905431 36.0 35.0 38.0 31.0 39.5 82-83 34.72948549920414 35.0 35.0 37.0 31.0 39.0 84-85 34.38769093444706 35.0 34.0 37.0 30.5 39.0 86-87 34.082417602476674 35.0 34.0 36.5 30.0 37.0 88-89 33.79825954854773 35.0 34.0 36.0 30.0 37.0 90-91 33.58615278041444 35.0 34.0 36.0 30.0 37.0 92-93 33.421483456607135 35.0 34.0 35.0 29.0 36.0 94-95 33.26487477000248 35.0 34.0 35.0 29.0 36.0 96-97 33.15049253238219 35.0 34.0 35.0 29.0 36.0 98-99 33.04598421596402 35.0 34.0 35.0 29.0 36.0 100-101 32.629995600841134 35.0 33.5 35.0 28.0 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17266805889971693 1101 2 0.18442545098165652 1101 3 0.16307023949332944 1101 4 0.15811774357901953 1101 5 0.28510180278559716 1101 6 0.13529570666847945 1101 7 0.31406721993578657 1101 8 0.18763583134141015 1101 9 0.23035624766637852 1101 10-11 0.1519864221189593 1101 12-13 0.1217908262193248 1101 14-15 0.10860816369086734 1101 16-17 0.19242414827013476 1101 18-19 0.12550667762986478 1101 20-21 0.1944144917192645 1101 22-23 0.2359219771903085 1101 24-25 0.19101330269580075 1101 26-27 0.11190310250415081 1101 28-29 0.15863774481223913 1101 30-31 -0.007589821456541301 1101 32-33 -0.037050445043533387 1101 34-35 0.04479520723772623 1101 36-37 0.06950496777178472 1101 38-39 -0.07200566523302854 1101 40-41 -0.057059057064570595 1101 42-43 -0.0036945804313432973 1101 44-45 0.08404328887627344 1101 46-47 0.17466222966142908 1101 48-49 0.11174450665340174 1101 50-51 0.24791750956797642 1101 52-53 -0.010475569774335725 1101 54-55 0.1899851894766016 1101 56-57 0.23528355669598966 1101 58-59 0.11353808946620347 1101 60-61 0.032075725389063336 1101 62-63 0.13426506134960192 1101 64-65 0.16753730495977237 1101 66-67 0.18235321654211845 1101 68-69 0.15152416513328149 1101 70-71 0.10932509803324564 1101 72-73 0.041320600132927154 1101 74-75 0.12185720663514843 1101 76-77 0.12317983807113819 1101 78-79 0.2358140288081927 1101 80-81 0.1272562512865747 1101 82-83 0.036211647265091074 1101 84-85 0.1319434399841768 1101 86-87 0.020614595165959315 1101 88-89 -0.009280218684942554 1101 90-91 0.07813745444082087 1101 92-93 -0.043037146045122654 1101 94-95 -0.01630632833484924 1101 96-97 -0.09240817952635894 1101 98-99 -0.1783676822758835 1101 100-101 -0.07147282896904272 1102 1 0.09777164237687685 1102 2 0.09929997816593072 1102 3 0.13536880044972577 1102 4 0.14056177242002832 1102 5 0.0055406719967976414 1102 6 -0.011202901193641424 1102 7 0.128754124218041 1102 8 0.03227865626880089 1102 9 0.16753144055114433 1102 10-11 0.1591750252303754 1102 12-13 0.11240396824143772 1102 14-15 0.11683590902885754 1102 16-17 0.18714815618396585 1102 18-19 0.21099206168552342 1102 20-21 0.10640672220677772 1102 22-23 0.15077575761137751 1102 24-25 0.17692625048010768 1102 26-27 0.08057188193298259 1102 28-29 -0.28746817793764023 1102 30-31 -0.1532808089423341 1102 32-33 0.11182351686661463 1102 34-35 0.06567005915394475 1102 36-37 0.17442766352801442 1102 38-39 0.1128618260924128 1102 40-41 0.08348192230292995 1102 42-43 0.1551158783427269 1102 44-45 0.18383702395514234 1102 46-47 0.1962259342515793 1102 48-49 0.061861169611709954 1102 50-51 0.16675526987971523 1102 52-53 0.08364708268430832 1102 54-55 -0.09678168305408974 1102 56-57 -0.13451757505497852 1102 58-59 -0.025069532112837578 1102 60-61 0.1681248801839672 1102 62-63 0.1882234836334291 1102 64-65 0.2560156351159364 1102 66-67 0.26786415820462395 1102 68-69 0.18293145716953063 1102 70-71 0.2557509418852888 1102 72-73 0.3047927663956784 1102 74-75 0.3118804189557167 1102 76-77 0.21028707778946654 1102 78-79 0.0011985054184719957 1102 80-81 -0.053467299597123485 1102 82-83 -0.20413509429037902 1102 84-85 -0.06569383443235921 1102 86-87 -0.06716706469536149 1102 88-89 -0.046615372120790255 1102 90-91 0.14379013558411913 1102 92-93 0.17397640249328106 1102 94-95 0.19997326587048292 1102 96-97 0.22245913139244777 1102 98-99 0.13331725557403473 1102 100-101 0.09632608979385537 1103 1 0.18796935548723326 1103 2 0.2175218826870875 1103 3 0.221106794167504 1103 4 0.1583308971219708 1103 5 0.03177779491976196 1103 6 0.07208844091292832 1103 7 0.1722508968923293 1103 8 0.0812270341239909 1103 9 0.24420630457490233 1103 10-11 0.18189785913874346 1103 12-13 0.10993792533282232 1103 14-15 0.18123733734439185 1103 16-17 0.23869859504111446 1103 18-19 0.20027969517158084 1103 20-21 0.051220345332588124 1103 22-23 -0.0033480877693889965 1103 24-25 0.1668111080252217 1103 26-27 0.07913190371819923 1103 28-29 0.12677722716190942 1103 30-31 0.11093817377067694 1103 32-33 0.180545437849446 1103 34-35 0.1120060525826645 1103 36-37 0.203661230170745 1103 38-39 0.21728814781161532 1103 40-41 0.21307019623192502 1103 42-43 0.1985681486869879 1103 44-45 0.18576415620267284 1103 46-47 0.1679884385692887 1103 48-49 0.06660171405238202 1103 50-51 0.20147755022932756 1103 52-53 0.1669617222489208 1103 54-55 0.036305394752730535 1103 56-57 0.06011217950979386 1103 58-59 0.15580258521036683 1103 60-61 0.09566267089299885 1103 62-63 0.0641236443998352 1103 64-65 0.17690737252596733 1103 66-67 0.1435814269501492 1103 68-69 0.20310384719855534 1103 70-71 0.11626431741632359 1103 72-73 0.13360667188110398 1103 74-75 0.18502827104169484 1103 76-77 0.18997755362121183 1103 78-79 0.27737187421973886 1103 80-81 0.33733137632602705 1103 82-83 0.17670329054568867 1103 84-85 0.13242984130943825 1103 86-87 0.06670166375449327 1103 88-89 0.18917485254483069 1103 90-91 0.16881300057437443 1103 92-93 0.12191690720270998 1103 94-95 0.056463016942032596 1103 96-97 0.17422083773734443 1103 98-99 0.09383503948634342 1103 100-101 0.21096995117871842 1104 1 0.07937342057714858 1104 2 0.0605902917275003 1104 3 0.10441972799365828 1104 4 0.109916336048002 1104 5 0.019346887310007332 1104 6 0.10557648734032199 1104 7 0.09208797013531012 1104 8 0.14244683039822092 1104 9 0.12792426623998665 1104 10-11 0.06491321599547462 1104 12-13 0.12479307536682427 1104 14-15 0.07963769282095257 1104 16-17 0.18962142027187667 1104 18-19 0.17813310148533645 1104 20-21 0.04724116225542474 1104 22-23 0.1280325481835618 1104 24-25 0.0620602445807279 1104 26-27 0.09865780032030358 1104 28-29 0.04210796174614018 1104 30-31 -0.055761482198029455 1104 32-33 -5.9949505548217985E-5 1104 34-35 0.06300024242600699 1104 36-37 -0.08646325395062604 1104 38-39 -0.07013161749306107 1104 40-41 0.020762897517165868 1104 42-43 0.10832167147317762 1104 44-45 -0.09836112235495165 1104 46-47 0.12260970118432368 1104 48-49 0.09427516035724892 1104 50-51 0.1490754577650364 1104 52-53 0.1717343990278124 1104 54-55 0.20737835497032364 1104 56-57 0.06847516955240707 1104 58-59 0.007167197788042756 1104 60-61 0.15053034339675264 1104 62-63 0.16852320544014532 1104 64-65 0.0927437186578004 1104 66-67 -0.016205152463179218 1104 68-69 0.10110698454850109 1104 70-71 0.04882085367425759 1104 72-73 0.05824362124588589 1104 74-75 -0.035086854889918584 1104 76-77 0.025155460214357106 1104 78-79 0.08352134942053624 1104 80-81 0.14448399560928493 1104 82-83 0.07603461759084951 1104 84-85 0.13590556724238922 1104 86-87 0.02967285534153774 1104 88-89 0.09179864658386805 1104 90-91 0.0867865148775877 1104 92-93 0.11817376594790119 1104 94-95 0.15224913854464006 1104 96-97 0.20479707332135177 1104 98-99 0.25051645035127024 1104 100-101 0.07845461788578234 1105 1 0.20265684073022072 1105 2 0.14925466208286764 1105 3 0.13430645927948603 1105 4 0.15071712559564787 1105 5 0.2511928470875233 1105 6 0.1215423433263112 1105 7 0.21555622809778185 1105 8 0.17871190402615156 1105 9 0.20808269864804174 1105 10-11 0.18055606333435037 1105 12-13 0.16598001122914496 1105 14-15 0.16445327985093883 1105 16-17 0.08752428238207699 1105 18-19 0.11317189258854654 1105 20-21 0.15971000192104867 1105 22-23 0.09370173556387584 1105 24-25 0.2633679655462302 1105 26-27 0.22557549259280307 1105 28-29 0.21237404191660403 1105 30-31 0.10137810694961757 1105 32-33 0.2733475891058106 1105 34-35 0.2572625578659782 1105 36-37 0.21390263079371863 1105 38-39 0.17280108141346773 1105 40-41 0.1773517641110658 1105 42-43 0.1400652776405309 1105 44-45 0.13337202622771827 1105 46-47 0.22895170341963222 1105 48-49 0.32871290178039203 1105 50-51 0.37217377866909374 1105 52-53 0.27845274359216177 1105 54-55 0.303631010216435 1105 56-57 0.0949979572830486 1105 58-59 0.1339280262901923 1105 60-61 0.1984588919493575 1105 62-63 0.2557176315475047 1105 64-65 0.14354954091808025 1105 66-67 0.22626532120004583 1105 68-69 0.14685695677054156 1105 70-71 0.16622188243914593 1105 72-73 0.2462494252053915 1105 74-75 0.25471294772317066 1105 76-77 0.2443795074303452 1105 78-79 0.19526456342847354 1105 80-81 0.1570860387598927 1105 82-83 0.15489369510116546 1105 84-85 0.07920075217824518 1105 86-87 0.22400745883567907 1105 88-89 0.2494483044247673 1105 90-91 0.16909698749505964 1105 92-93 0.17462472314370814 1105 94-95 0.13213985678462592 1105 96-97 0.1595206989659701 1105 98-99 0.22978376582391746 1105 100-101 0.2502675055517116 1106 1 0.11854351877163793 1106 2 0.15743539784089933 1106 3 0.12773300008741728 1106 4 0.1175945115310526 1106 5 0.13122525284691022 1106 6 -0.03671682909933338 1106 7 0.08943988651161305 1106 8 0.1287122722734182 1106 9 0.1407909117895585 1106 10-11 0.1527085913518249 1106 12-13 0.14040916659266856 1106 14-15 0.09277358132269597 1106 16-17 0.019893830788177524 1106 18-19 0.06405961399720894 1106 20-21 0.07986073780183034 1106 22-23 0.11581842084748217 1106 24-25 -0.010616347120254943 1106 26-27 0.022050261517641445 1106 28-29 0.041788037767688024 1106 30-31 -0.026071903484059078 1106 32-33 0.08969298049127872 1106 34-35 -0.07211854122936501 1106 36-37 0.0054594574842781185 1106 38-39 -0.024388823701485762 1106 40-41 0.009372336858312735 1106 42-43 0.07078286881241524 1106 44-45 0.005810559621608036 1106 46-47 -0.05398199979762097 1106 48-49 8.288505694693526E-4 1106 50-51 0.10974064623509605 1106 52-53 -0.03903531905897495 1106 54-55 -0.022434758745234262 1106 56-57 -0.020552388255637766 1106 58-59 -0.008905022674419172 1106 60-61 -0.030258380074897673 1106 62-63 0.03317635716646805 1106 64-65 0.15212921121402445 1106 66-67 0.1024581354684031 1106 68-69 -0.10639380734252768 1106 70-71 -0.046690707934864406 1106 72-73 0.05926991202827736 1106 74-75 0.08177408904419536 1106 76-77 0.03768317640060559 1106 78-79 0.03964425090929069 1106 80-81 0.04708456577083808 1106 82-83 0.12923385737545345 1106 84-85 0.06961478485404626 1106 86-87 0.0594986393136665 1106 88-89 0.09940080052748357 1106 90-91 0.1155226567844636 1106 92-93 0.12464509949241176 1106 94-95 0.06866028837042393 1106 96-97 0.011968438541501314 1106 98-99 -0.015923207551800544 1106 100-101 0.05775458621014451 1107 1 0.12345216954353333 1107 2 0.1312994701894894 1107 3 0.08836787722270856 1107 4 0.11326912558056534 1107 5 0.09046294353842654 1107 6 0.11233313892843455 1107 7 0.2038429193059912 1107 8 0.1557169310840507 1107 9 0.18901965025671785 1107 10-11 0.10528920704562239 1107 12-13 0.09198311379281421 1107 14-15 0.16595390469896643 1107 16-17 0.17286610629745525 1107 18-19 0.06736348720448149 1107 20-21 0.03923590908163277 1107 22-23 0.14518902466588202 1107 24-25 0.17390384105916468 1107 26-27 0.1614608306233336 1107 28-29 0.10700727591512305 1107 30-31 0.13290041769982253 1107 32-33 0.27947237210541687 1107 34-35 0.2594885200659647 1107 36-37 0.26731453193389854 1107 38-39 0.11459514450898212 1107 40-41 0.1701337326745218 1107 42-43 0.14841189959710732 1107 44-45 0.29745895644265374 1107 46-47 0.35903389496807137 1107 48-49 0.2532032453808384 1107 50-51 0.1868384812583912 1107 52-53 0.16856607858421313 1107 54-55 0.28773355220754127 1107 56-57 0.17610674209971222 1107 58-59 0.14397978594894312 1107 60-61 0.19831999756280538 1107 62-63 0.0610671034896626 1107 64-65 -0.04077615098565701 1107 66-67 0.22392520407166927 1107 68-69 0.26357901626211344 1107 70-71 0.3147701945955106 1107 72-73 0.38522085749279 1107 74-75 0.23528281289547692 1107 76-77 0.20091899443294636 1107 78-79 0.23436951810578677 1107 80-81 0.22308278446088536 1107 82-83 0.15884859200595258 1107 84-85 0.27551388963161116 1107 86-87 0.06612282858926477 1107 88-89 0.05709194878728141 1107 90-91 0.17800118971585732 1107 92-93 0.21941786828751475 1107 94-95 0.22179836272869125 1107 96-97 0.15112094607781046 1107 98-99 0.24942665999181912 1107 100-101 0.3266707276187404 1108 1 0.11642969763342137 1108 2 0.08271264148470436 1108 3 0.01149690162684891 1108 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-0.9484118137368966 2316 62-63 -0.9705409927729889 2316 64-65 -0.9161427140584166 2316 66-67 -0.9311130459880914 2316 68-69 -1.0312960733864713 2316 70-71 -1.0841928701905346 2316 72-73 -1.3110457772988227 2316 74-75 -1.0115177289803725 2316 76-77 -0.9757375120615919 2316 78-79 -1.0113183853128191 2316 80-81 -1.2789801289186684 2316 82-83 -1.5490155804380663 2316 84-85 -1.5234192922952374 2316 86-87 -1.121163010919858 2316 88-89 -1.0011856032088886 2316 90-91 -0.8165790557220163 2316 92-93 -0.7453133851988198 2316 94-95 -0.9322746709791687 2316 96-97 -0.8448478562171928 2316 98-99 -0.8197322371314613 2316 100-101 -0.970990440601831 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 4.0 13 22.0 14 77.0 15 227.0 16 628.0 17 1192.0 18 2003.0 19 2995.0 20 4400.0 21 5730.0 22 6620.0 23 7039.0 24 7237.0 25 8218.0 26 9804.0 27 12501.0 28 16003.0 29 20009.0 30 25639.0 31 32294.0 32 41287.0 33 54082.0 34 75481.0 35 113316.0 36 197405.0 37 405430.0 38 734025.0 39 407288.0 40 370.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.66142385844509 21.728921399880015 14.43289555504218 22.17675918663271 2 14.075036804670107 21.07622970495113 43.39012767775081 21.458605812627948 3 17.765883363345715 26.361167114950913 31.791186856494587 24.081762665208792 4 11.299481441521372 17.553765870071025 41.67727790115715 29.469474787250455 5 11.28484644927642 39.35462879130828 37.746380277165265 11.614144482250033 6 28.73097957037924 40.6449878794959 18.68592926298573 11.938103287139123 7 25.305111786095008 33.89980870047752 24.033554082273398 16.761525431154077 8 22.928288234349218 39.7339877918778 21.269157332904978 16.068566640868003 9 24.01511777333197 17.38151477095168 22.239573445873916 36.36379400984243 10-11 22.865061734254297 28.198904956263966 30.685205500956496 18.25082780852524 12-13 23.0067794506345 25.263310649980287 31.177144635581712 20.5527652638035 14-15 20.1870737744418 27.79768706464756 27.867233933030565 24.14800522788008 16-17 20.185887279311906 31.176414484732547 28.51743326421239 20.12026497174316 18-19 19.994770294542853 30.227218380817476 30.230869135063305 19.547142189576366 20-21 20.635911191752214 29.330661589684432 30.345388732312095 19.688038486251262 22-23 20.36374522455188 29.1112549636937 30.100331471945818 20.4246683398086 24-25 20.32500383329196 29.243613918135487 30.038154945311703 20.39322730326085 26-27 20.42836581287694 29.40655164356956 29.8032745440208 20.3618079995327 28-29 20.295514629898083 29.39524551134351 30.03387716947166 20.275362689286744 30-31 20.54216764361212 29.158290267478044 29.953045000348027 20.346497088561808 32-33 20.329863899881715 29.280349632734122 29.95635523299114 20.433431234393026 34-35 20.535789256560406 29.327489996933366 29.827962769608202 20.308757976898026 36-37 20.601753822339695 29.10509061172038 29.83619978387535 20.456955782064576 38-39 20.478426780472848 29.386769118999982 29.782328341535365 20.352475758991808 40-41 20.612728902291213 29.177147373647394 29.900521510244015 20.309602213817374 42-43 20.484724331546897 29.186593700258474 29.793326238700917 20.535355729493713 44-45 20.67342269162809 29.302824588559993 29.528304297667894 20.495448422144015 46-47 20.577065596752288 29.176462857226305 29.490587442865696 20.75588410315571 48-49 20.548277263845193 29.163293306469708 29.67580819699708 20.612621232688014 50-51 20.584975424259277 29.192634602925903 29.50820906642764 20.714180906387185 52-53 20.620304217351304 29.308597343711213 29.513724097898624 20.55737434103886 54-55 20.523220553156943 29.268411221947755 29.470641574252515 20.737726650642788 56-57 20.657382190160487 29.209730355291402 29.37622756611516 20.75665988843295 58-59 20.657245286876268 29.086061055214007 29.45665824559354 20.800035412316184 60-61 20.649099541465265 29.223945479636093 29.4097688707487 20.717186108149942 62-63 20.439956957607443 29.310240183121834 29.360004526935263 20.88979833233546 64-65 20.54603418566276 29.1680661224609 29.417412637450894 20.868487054425444 66-67 20.62226649775844 29.219655843397245 29.212308700477518 20.9457689583668 68-69 20.539280290307975 29.16049080740081 29.282152192643 21.018076709648213 70-71 20.474935746725272 28.860330356751707 29.48360537537055 21.18112852115247 72-73 20.516143635275046 29.110589560303158 29.284684903401043 21.08858190102075 74-75 20.428114823522538 28.984227828969466 29.26697874530878 21.320678602199212 76-77 20.370273185940217 29.029656902115974 29.30814099943048 21.291928912513324 78-79 20.51527775686195 28.940986791997346 29.11325715104528 21.43047830009542 80-81 20.478421163607752 28.962403050182512 29.114816332752497 21.444359453457242 82-83 20.458494069733852 29.002718580587867 29.156970760997197 21.381816588681087 84-85 20.589432526760028 28.81843795178084 29.129025869244586 21.463103652214546 86-87 20.29438313205508 28.90829214065626 29.26271192628397 21.534612801004688 88-89 20.565771075804264 28.692441295871728 29.09176535872311 21.6500222696009 90-91 20.464987441405395 28.77424100819229 29.09797164094102 21.662799909461295 92-93 20.519041421457672 28.722719738898057 29.05069437345756 21.707544466186715 94-95 20.418143700988626 28.56644463996262 29.154010718614465 21.861400940434294 96-97 20.33659497802246 28.42821795732998 29.2299007724996 22.005286292147957 98-99 20.36748948582777 28.507507776106543 28.981352860000875 22.143649878064807 100-101 20.26640922764643 28.55768282977263 28.989818046408388 22.18608989617255 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 27.0 1 21.5 2 24.0 3 57.5 4 60.5 5 42.0 6 72.0 7 164.0 8 300.5 9 395.0 10 381.0 11 331.0 12 349.5 13 420.0 14 492.5 15 569.0 16 639.5 17 702.0 18 836.5 19 941.5 20 1016.0 21 1256.0 22 1527.0 23 1955.5 24 2588.0 25 3348.5 26 4940.5 27 8190.5 28 14893.0 29 26259.0 30 40893.0 31 58140.5 32 77391.5 33 93733.5 34 108479.0 35 119729.0 36 125302.5 37 126454.0 38 116963.0 39 103799.5 40 91854.5 41 79870.0 42 70511.0 43 64170.0 44 59922.5 45 56605.5 46 54757.0 47 54413.5 48 54819.5 49 54587.0 50 53356.0 51 51965.0 52 50224.0 53 48652.5 54 46591.0 55 43051.0 56 38996.0 57 34982.5 58 30907.5 59 26131.0 60 21240.5 61 16375.0 62 12337.5 63 9177.0 64 6609.5 65 4687.5 66 3158.0 67 2277.5 68 1677.5 69 1153.5 70 800.5 71 525.5 72 388.5 73 280.5 74 184.5 75 139.5 76 92.0 77 59.0 78 37.0 79 30.0 80 22.0 81 13.0 82 9.0 83 4.0 84 2.5 85 3.0 86 1.5 87 0.0 88 0.0 89 0.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046820923202733684 2 0.0013690328421851953 3 4.563442807283985E-4 4 0.011591144730501322 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0018938287650228538 24-25 0.0 26-27 0.0 28-29 0.02153945005038041 30-31 0.01827658844317236 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03456807926517618 50-51 0.011157617663809342 52-53 0.0 54-55 6.845164210925977E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.2817214036419925E-4 80-81 0.011226069305918603 82-83 0.005065421516085223 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2191328.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.36202186236371 #Duplication Level Percentage of deduplicated Percentage of total 1 67.34957215128097 33.24511052952373 2 16.00089930785671 15.796734829038064 3 6.413209792191571 9.49707005910256 4 3.268986877752056 6.454552069095082 5 1.92972179236198 4.762748465142588 6 1.2337215890301303 3.6539395229865232 7 0.8226889439969258 2.842671274765082 8 0.5930994077881949 2.3421268747036676 9 0.41097117726987786 1.8257731413477358 >10 1.8945119185869563 15.471499859403862 >50 0.060662619460893255 2.005729764735958 >100 0.021211023902368484 1.7687553320319707 >500 5.575488911681594E-4 0.17338696549761606 >1k 1.85849630389307E-4 0.15990131262552354 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.563442807283985E-5 0.0 9 0.0 0.0 0.0 9.12688561456797E-5 0.0 10-11 0.0 0.0 0.0 1.1408607018209963E-4 0.0 12-13 0.0 0.0 0.0 3.878926386191387E-4 0.0 14-15 0.0 0.0 0.0 6.616992070561778E-4 0.0 16-17 0.0 0.0 0.0 0.0015743877685129746 0.0 18-19 0.0 0.0 0.0 0.0023729902597876724 0.0 20-21 0.0 0.0 0.0 0.0041070985265555865 0.0 22-23 0.0 0.0 0.0 0.007963207698710553 0.0 24-25 0.0 0.0 0.0 0.0117964996568291 0.0 26-27 0.0 0.0 0.0 0.013872866134143316 0.0 28-29 0.0 0.0 0.0 0.016839103958877902 0.0 30-31 0.0 0.0 0.0 0.02377553702594956 0.0 32-33 0.0 0.0 0.0 0.03954223192511573 0.0 34-35 0.0 0.0 0.0 0.0628614246703369 0.0 36-37 0.0 0.0 0.0 0.09818247199871494 0.0 38-39 0.0 0.0 0.0 0.1554080448020561 0.0 40-41 0.0 0.0 0.0 0.22144106222345536 0.0 42-43 0.0 0.0 0.0 0.28532926152543114 0.0 44-45 0.0 0.0 0.0 0.36413991880722557 0.0 46-47 0.0 0.0 0.0 0.46031447597073555 4.563442807283985E-5 48-49 0.0 0.0 0.0 0.5591358299624702 4.563442807283985E-5 50-51 0.0 0.0 0.0 0.6644600899545846 4.563442807283985E-5 52-53 0.0 0.0 0.0 0.7738914484732546 4.563442807283985E-5 54-55 0.0 0.0 0.0 0.894594510725916 4.563442807283985E-5 56-57 0.0 0.0 0.0 1.040921304341477 4.563442807283985E-5 58-59 0.0 0.0 0.0 1.1963065319294968 4.563442807283985E-5 60-61 0.0 0.0 0.0 1.3590845368653164 4.563442807283985E-5 62-63 0.0 0.0 0.0 1.5269051461031848 9.12688561456797E-5 64-65 0.0 0.0 0.0 1.7000649834255757 9.12688561456797E-5 66-67 0.0 0.0 0.0 1.9049407482585903 9.12688561456797E-5 68-69 0.0 0.0 0.0 2.141235816819755 1.3690328421851956E-4 70-71 0.0 0.0 0.0 2.4030405306736373 1.3690328421851956E-4 72-73 0.0 0.0 0.0 2.668724170913711 1.3690328421851956E-4 74-75 0.0 0.0 0.0 2.928041808437623 1.3690328421851956E-4 76-77 0.0 0.0 0.0 3.22149399816002 1.3690328421851956E-4 78-79 0.0 0.0 0.0 3.568178748229384 1.3690328421851956E-4 80-81 0.0 0.0 0.0 3.9362660450649107 1.3690328421851956E-4 82-83 0.0 0.0 0.0 4.329201287986098 1.3690328421851956E-4 84-85 2.2817214036419924E-5 0.0 0.0 4.704407555600987 1.3690328421851956E-4 86-87 4.563442807283985E-5 0.0 0.0 5.115071773828473 1.3690328421851956E-4 88-89 4.563442807283985E-5 0.0 0.0 5.5716670439112725 1.3690328421851956E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2290 0.0 35.071865 1 GTATCAA 3895 0.0 32.82105 1 TCAACGC 3705 0.0 31.66637 4 ATCAACG 3800 0.0 30.875416 3 CAACGCA 3795 0.0 30.537111 5 AACGCAG 3860 0.0 30.145931 6 TATCAAC 4470 0.0 27.098299 2 ACGCAGA 4480 0.0 25.549883 7 CGCAGAG 4545 0.0 24.975483 8 GTACATG 4665 0.0 24.347471 1 ACATGGG 4365 0.0 23.831936 3 TACATGG 5040 0.0 21.865871 2 GCAGAGT 5365 0.0 21.423752 9 TCGTTAT 190 8.221767E-6 20.00986 1 CATGGGG 2530 0.0 19.901 4 GAGTACA 3525 0.0 19.279001 1 GTATATA 2180 0.0 19.183764 1 TAATACT 1975 0.0 18.518814 4 GTATAGG 920 0.0 18.07956 1 AGAGTAC 5095 0.0 18.037928 10-11 >>END_MODULE