FastQCFastQC Report
Fri 10 Feb 2017
SRR2031663_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031663_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372011
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23200.16909485419577538No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18970.13826419759025257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42400.024.13081
GTATCAA58550.023.814411
GTACATG46600.022.058041
TCAACGC63250.021.4162644
CAACGCA63900.021.2727955
GTATAGA4950.021.1503031
ATCAACG64050.021.1472243
TACATGG47250.021.1371612
AACGCAG65000.021.1244416
GTATAGG3850.021.0129621
TATCAAC67300.020.4226442
ACATGGG47300.020.0953923
AGAGTAC50500.019.52178810-11
GAGTACT26550.019.4155712-13
CGCAGAG73100.018.719398
ACGCAGA73250.018.6796957
ACTCTCG1801.2308646E-418.4774558
TCTATAC4303.6379788E-1217.6839453
CATGGGG27000.017.6033634
GTATATA9250.017.491871
Produced by FastQC (version 0.11.5)