##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031663_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1372011 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.815322180361527 33.0 31.0 34.0 30.0 34.0 2 32.07527199125955 34.0 31.0 34.0 30.0 34.0 3 32.220764265009535 34.0 31.0 34.0 30.0 34.0 4 35.31711844875879 37.0 35.0 37.0 33.0 37.0 5 35.01202468493329 37.0 35.0 37.0 32.0 37.0 6 35.23461911019664 37.0 35.0 37.0 32.0 37.0 7 35.38056327536732 37.0 35.0 37.0 33.0 37.0 8 35.46345109477985 37.0 35.0 37.0 33.0 37.0 9 37.37703925114303 39.0 38.0 39.0 34.0 39.0 10-11 37.329769221966885 39.0 37.5 39.0 34.5 39.0 12-13 37.35594065936789 39.0 38.0 39.0 34.5 39.0 14-15 38.74419046202982 41.0 38.5 41.0 34.5 41.0 16-17 38.69354910419814 41.0 38.0 41.0 34.0 41.0 18-19 38.62501211724979 41.0 38.5 41.0 34.0 41.0 20-21 38.62234158472491 40.5 39.0 41.0 34.0 41.0 22-23 38.55526048989403 40.0 38.0 41.0 34.0 41.0 24-25 38.47541747114273 40.0 38.0 41.0 34.0 41.0 26-27 38.37340225406356 40.0 38.0 41.0 34.0 41.0 28-29 38.259141508340676 40.0 38.0 41.0 33.5 41.0 30-31 38.12048263461445 40.0 38.0 41.0 33.0 41.0 32-33 37.965146052036026 40.0 38.0 41.0 33.0 41.0 34-35 37.82843176913305 40.0 38.0 41.0 33.0 41.0 36-37 37.692622362357156 40.0 38.0 41.0 32.0 41.0 38-39 37.51780087768975 40.0 37.0 41.0 31.0 41.0 40-41 37.376253907585294 40.0 37.0 41.0 31.0 41.0 42-43 37.19717881270631 40.0 37.0 41.0 31.0 41.0 44-45 36.97757816810507 40.0 36.5 41.0 30.0 41.0 46-47 36.75034784706536 40.0 36.0 41.0 30.0 41.0 48-49 36.87086473796493 40.0 36.5 41.0 30.0 41.0 50-51 36.63789976902518 39.5 36.5 40.5 30.0 41.0 52-53 36.76879449217244 39.5 36.5 40.5 30.0 41.0 54-55 37.033099224423125 40.0 36.5 41.0 30.0 41.0 56-57 36.92321453690969 40.0 36.0 41.0 30.0 41.0 58-59 36.722694278690184 39.5 36.0 41.0 29.5 41.0 60-61 36.42356621047499 39.0 35.0 41.0 29.0 41.0 62-63 36.088639231026576 39.0 35.0 41.0 28.0 41.0 64-65 35.81791290303066 38.5 35.0 41.0 28.0 41.0 66-67 35.592561940101064 38.0 35.0 40.5 28.0 41.0 68-69 35.33411175274834 37.0 35.0 40.0 28.0 41.0 70-71 35.01907236895331 37.0 34.0 39.5 27.5 41.0 72-73 34.65922175551071 36.0 34.0 39.0 26.5 41.0 74-75 34.24906214308777 36.0 34.0 39.0 26.0 41.0 76-77 33.85201139057923 35.0 34.0 37.5 26.0 40.0 78-79 33.504438375494075 35.0 34.0 37.0 26.0 39.0 80-81 33.17923471459048 35.0 33.0 37.0 26.0 39.0 82-83 32.85737978777138 35.0 33.0 36.0 25.0 38.5 84-85 32.59934104026863 35.0 33.0 36.0 25.0 37.0 86-87 32.37150248795382 35.0 33.0 35.5 25.0 37.0 88-89 32.12434411969 35.0 33.0 35.0 24.0 36.5 90-91 31.92973270622466 35.0 32.0 35.0 24.0 36.0 92-93 31.754269098425596 35.0 32.0 35.0 23.0 36.0 94-95 31.603354127627256 35.0 32.0 35.0 23.0 36.0 96-97 31.510487525245786 35.0 32.0 35.0 23.0 35.0 98-99 31.398379094628247 35.0 32.0 35.0 20.5 35.0 100-101 30.93979348562074 34.5 31.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14137517713022874 1101 2 0.25184521735882015 1101 3 0.30088185900577713 1101 4 0.028134226878606228 1101 5 0.25364190230462924 1101 6 0.2766841972694962 1101 7 0.0488993883879445 1101 8 0.2132922282342875 1101 9 0.14911399818589643 1101 10-11 0.3071766431395915 1101 12-13 0.27811245922050887 1101 14-15 0.2953861359649892 1101 16-17 0.34618293453117843 1101 18-19 0.3868655290423604 1101 20-21 0.14964995080173793 1101 22-23 0.09193539200817469 1101 24-25 0.26609252000199746 1101 26-27 0.1710551551623567 1101 28-29 0.41700531248740447 1101 30-31 0.2242904741730669 1101 32-33 0.3429190052340587 1101 34-35 0.34869796679823395 1101 36-37 0.24998838610233065 1101 38-39 0.17989122999875917 1101 40-41 0.22266328753413234 1101 42-43 -0.024773753661932574 1101 44-45 0.22063101031387333 1101 46-47 0.4092965408757152 1101 48-49 0.5611182000665593 1101 50-51 0.4687882638332539 1101 52-53 0.41443022409522 1101 54-55 0.365717901287006 1101 56-57 0.31882052499292257 1101 58-59 0.5593362123015098 1101 60-61 0.5217849005652511 1101 62-63 0.15998877059612937 1101 64-65 0.24167756794109607 1101 66-67 0.394831203648323 1101 68-69 0.28097563101999157 1101 70-71 0.21657296674646886 1101 72-73 0.2811637787636698 1101 74-75 0.010999409800028559 1101 76-77 0.06515070416312341 1101 78-79 0.2318748671345645 1101 80-81 0.10617300227125526 1101 82-83 0.17445759593378085 1101 84-85 0.30815466598490104 1101 86-87 0.18408587143891708 1101 88-89 0.31106971073864287 1101 90-91 0.10664505517901546 1101 92-93 0.17428420500573694 1101 94-95 -0.09162965238752818 1101 96-97 0.23365487610025681 1101 98-99 0.31296383746498435 1101 100-101 0.2132626106897817 1102 1 0.13971542609288434 1102 2 0.11304159810237735 1102 3 0.060164530150252915 1102 4 0.2045896314776101 1102 5 0.22824465837950925 1102 6 0.2402723680739811 1102 7 0.2521599110152337 1102 8 0.23628102907804305 1102 9 0.4943387445742715 1102 10-11 0.3315289100319987 1102 12-13 0.2701058257673594 1102 14-15 0.3689418333796084 1102 16-17 0.36416819660809097 1102 18-19 0.6401891943721623 1102 20-21 0.3828866040997312 1102 22-23 0.1355617953720767 1102 24-25 0.330746137176817 1102 26-27 0.31833550876148564 1102 28-29 0.16990675436142055 1102 30-31 0.37849189245530823 1102 32-33 0.3338503947898417 1102 34-35 0.021107728243713098 1102 36-37 -0.04226206144759459 1102 38-39 0.013905024250689735 1102 40-41 0.24544323174650629 1102 42-43 0.15471805166426122 1102 44-45 -0.19922841043856465 1102 46-47 -0.6446717351203475 1102 48-49 -0.06110768427821256 1102 50-51 -0.08164506163615215 1102 52-53 -0.05104112577086539 1102 54-55 -0.2346658120590135 1102 56-57 -0.13591274912967322 1102 58-59 -0.0077175214444409335 1102 60-61 -0.3012864715048664 1102 62-63 -0.6504523262828172 1102 64-65 -0.20382923898100813 1102 66-67 -0.00941219412295169 1102 68-69 0.02095814534852991 1102 70-71 -0.05758432103160516 1102 72-73 0.08473272920704744 1102 74-75 0.005915034496453586 1102 76-77 -0.14891034303430928 1102 78-79 0.015035194088866888 1102 80-81 0.13369697568193573 1102 82-83 -0.16372361668365443 1102 84-85 0.06010931425922905 1102 86-87 0.013467697442614224 1102 88-89 -0.2027200310007693 1102 90-91 -0.20791280712825966 1102 92-93 -0.14070975827435106 1102 94-95 0.09352869008183973 1102 96-97 0.006348085831174188 1102 98-99 -0.045609692559025916 1102 100-101 0.1069497281907168 1103 1 0.15687273014817293 1103 2 0.048043256499617826 1103 3 0.12234901161343714 1103 4 0.11032227076605494 1103 5 0.13726430869460415 1103 6 0.224274588295593 1103 7 0.36572627736534 1103 8 0.3772441178936745 1103 9 0.38020649247081906 1103 10-11 0.3304079688746455 1103 12-13 0.3764050277059994 1103 14-15 0.4484166291259868 1103 16-17 0.4581248616727649 1103 18-19 0.31836367130013343 1103 20-21 0.07637688724567937 1103 22-23 0.28652434267024773 1103 24-25 0.3422415376628294 1103 26-27 0.22698997323455927 1103 28-29 0.12743553939929342 1103 30-31 0.3459944138960154 1103 32-33 0.247547402228939 1103 34-35 0.3719545261832522 1103 36-37 0.45468716452087676 1103 38-39 0.3280332149731464 1103 40-41 0.18215168551107297 1103 42-43 0.1833220196335006 1103 44-45 0.31027048283511505 1103 46-47 0.6227773114704647 1103 48-49 0.4008892967619033 1103 50-51 0.5373794623361476 1103 52-53 0.5286418157520174 1103 54-55 0.46655429448455266 1103 56-57 0.33036594283488796 1103 58-59 0.5972651161517675 1103 60-61 0.5079159409554563 1103 62-63 0.663437596754008 1103 64-65 0.6666756583675877 1103 66-67 0.2903904904498944 1103 68-69 0.4252902850871081 1103 70-71 0.44533987329194247 1103 72-73 0.536331221080907 1103 74-75 0.3536403226852798 1103 76-77 -0.15777904535539022 1103 78-79 0.1643332520718488 1103 80-81 0.4527374067298382 1103 82-83 0.2940650967385352 1103 84-85 0.21831059630184058 1103 86-87 0.2406522127506321 1103 88-89 0.45023056229930347 1103 90-91 0.4337461316328799 1103 92-93 0.3217346515731414 1103 94-95 0.2705666572252632 1103 96-97 0.294680753549283 1103 98-99 0.35039966215522256 1103 100-101 0.41879000761372964 1104 1 0.08142313876092544 1104 2 0.19675952003039043 1104 3 0.2010025651164753 1104 4 0.017261319853055568 1104 5 0.013904595160376232 1104 6 -0.05648872927221049 1104 7 0.08416816333454591 1104 8 0.32720845311391855 1104 9 0.32930496247132623 1104 10-11 0.3594411860203408 1104 12-13 0.31242815048020844 1104 14-15 0.39387657438348356 1104 16-17 0.2538100101282268 1104 18-19 0.4052087140261449 1104 20-21 0.32069171270993735 1104 22-23 0.39865356262217944 1104 24-25 0.37053505293218336 1104 26-27 0.43062575954841265 1104 28-29 0.33525232023237095 1104 30-31 0.24744519454392133 1104 32-33 0.19268167001862224 1104 34-35 0.270978536990917 1104 36-37 0.32553697732968345 1104 38-39 0.3858388464875162 1104 40-41 0.3285999428921116 1104 42-43 0.19623338711374316 1104 44-45 0.2427303876681819 1104 46-47 0.49209718998853447 1104 48-49 0.3579011750243879 1104 50-51 0.43894825624066414 1104 52-53 0.6101746319134449 1104 54-55 0.4610256016150345 1104 56-57 0.473457016468636 1104 58-59 0.4624815889767646 1104 60-61 0.45756470481548916 1104 62-63 0.5042481457047359 1104 64-65 0.7179664365117873 1104 66-67 0.5928744412990099 1104 68-69 0.3735985226732197 1104 70-71 0.27094953238358954 1104 72-73 0.30936630035843393 1104 74-75 0.3126721184558434 1104 76-77 0.2904463873490144 1104 78-79 0.3061666043133826 1104 80-81 0.3704639106816501 1104 82-83 0.42972361723045793 1104 84-85 0.3570306153925955 1104 86-87 0.19072771515457987 1104 88-89 0.26693478456586917 1104 90-91 0.2498351853491272 1104 92-93 0.14077990476700464 1104 94-95 0.09909502539533221 1104 96-97 0.20587642996084554 1104 98-99 0.31834080679253063 1104 100-101 0.29929278320728514 1105 1 -0.005022963845757289 1105 2 0.12528145848273198 1105 3 0.18201481186438428 1105 4 0.05324244974386971 1105 5 -0.1975457643041807 1105 6 -0.03894056795618184 1105 7 0.1651097617212116 1105 8 0.13933086982036258 1105 9 0.22051453547906874 1105 10-11 0.34515114632475985 1105 12-13 0.24023834229576835 1105 14-15 0.20620362985761886 1105 16-17 0.25266050370702686 1105 18-19 0.2169478587289717 1105 20-21 0.09609679530536397 1105 22-23 0.11333418063745171 1105 24-25 0.05434648558311039 1105 26-27 0.10417491402808565 1105 28-29 0.19912017250361913 1105 30-31 0.13256204297068308 1105 32-33 0.09490891997698014 1105 34-35 0.11344600376110492 1105 36-37 0.05269343961144557 1105 38-39 0.13997635754760296 1105 40-41 0.12091047866179849 1105 42-43 0.13645564772507868 1105 44-45 0.3634223701008281 1105 46-47 0.5077113446756272 1105 48-49 0.4263554743270035 1105 50-51 0.11696324161874116 1105 52-53 0.23646028038924527 1105 54-55 0.2750108193277683 1105 56-57 0.39846666018666355 1105 58-59 0.35032716069908787 1105 60-61 0.42753450743969523 1105 62-63 0.2451729890988048 1105 64-65 0.2780067079352406 1105 66-67 0.14853092308491256 1105 68-69 0.22348344819523902 1105 70-71 0.3306830004503496 1105 72-73 0.33104376860846685 1105 74-75 0.38512507785439 1105 76-77 0.28908233529089244 1105 78-79 0.3248500309260791 1105 80-81 0.2562983879487959 1105 82-83 0.2370882599856614 1105 84-85 0.42288126562376505 1105 86-87 0.40092764196758424 1105 88-89 0.339937884796754 1105 90-91 0.5074961363714223 1105 92-93 0.31393185570818716 1105 94-95 0.24932062338324457 1105 96-97 0.3930967543984316 1105 98-99 0.1930994042522869 1105 100-101 0.24715819343526135 1106 1 0.20193535881077196 1106 2 0.24786715776577495 1106 3 0.15603808250120466 1106 4 0.3560503945735931 1106 5 0.16192183617452827 1106 6 0.049034119670437804 1106 7 0.08406771379258515 1106 8 0.29090078463855207 1106 9 0.23881686846507932 1106 10-11 0.2776586181306655 1106 12-13 0.3031777158544031 1106 14-15 0.2968855827710186 1106 16-17 0.31666237774208383 1106 18-19 0.35405175919637344 1106 20-21 0.20228439465824977 1106 22-23 0.42099813964791366 1106 24-25 0.3501156579409752 1106 26-27 0.32452502318066223 1106 28-29 0.2445584862004324 1106 30-31 0.24570427085592428 1106 32-33 0.39961347506663714 1106 34-35 0.624854104213135 1106 36-37 0.38719726302996804 1106 38-39 0.5126633520609474 1106 40-41 0.4561757021181947 1106 42-43 0.2839110118572492 1106 44-45 0.5126383069043285 1106 46-47 0.710417613135256 1106 48-49 0.39350327623516534 1106 50-51 0.14811203800858408 1106 52-53 0.27950532935397376 1106 54-55 0.3593004714995942 1106 56-57 0.25067354755867655 1106 58-59 0.1257027153307888 1106 60-61 0.19121669314155554 1106 62-63 0.3285739991778982 1106 64-65 0.5383835320556472 1106 66-67 0.2903512819309668 1106 68-69 0.3727298761823903 1106 70-71 0.4242067903168376 1106 72-73 0.26846911651580285 1106 74-75 0.1613203762311528 1106 76-77 0.23114908635559317 1106 78-79 0.2585402088362727 1106 80-81 0.1899730270827007 1106 82-83 0.3561343433051647 1106 84-85 0.20101099505556164 1106 86-87 0.18468621981187283 1106 88-89 0.22808713444977258 1106 90-91 0.060865089661859884 1106 92-93 0.16562361798719039 1106 94-95 0.09210954640532165 1106 96-97 0.14331209838296033 1106 98-99 0.14805190543821567 1106 100-101 0.23766517386402697 1107 1 0.10742683764683392 1107 2 0.02117039723268732 1107 3 -0.00869063972924522 1107 4 0.06388439040030391 1107 5 0.04109701096942331 1107 6 -0.031911490694071176 1107 7 -0.05953030962140815 1107 8 0.13043362205625186 1107 9 0.15693156967265054 1107 10-11 0.1988759113028422 1107 12-13 0.07212386254524006 1107 14-15 0.10587435623180852 1107 16-17 0.06527980108513276 1107 18-19 0.16977226613648355 1107 20-21 0.16137414831127472 1107 22-23 0.18743360869601844 1107 24-25 0.08838114506216499 1107 26-27 0.05766272775981918 1107 28-29 0.19647655117972107 1107 30-31 0.08396417124448163 1107 32-33 0.12479678973097208 1107 34-35 0.11941122995093423 1107 36-37 0.05156899304552809 1107 38-39 0.19988900857186565 1107 40-41 0.07108669150061075 1107 42-43 0.15376109188719767 1107 44-45 0.14613607640132642 1107 46-47 0.1525477225514109 1107 48-49 0.1268116554960983 1107 50-51 0.026863459133700474 1107 52-53 0.25924504348086685 1107 54-55 0.23484315274018996 1107 56-57 0.3138904999401646 1107 58-59 0.2598560879016105 1107 60-61 0.3285614867257536 1107 62-63 0.3888151784254177 1107 64-65 0.38514811552282424 1107 66-67 0.16063573042269041 1107 68-69 0.3705164127154319 1107 70-71 0.36711289689493753 1107 72-73 0.34793647742711187 1107 74-75 0.48573160444637864 1107 76-77 0.563303525992346 1107 78-79 0.45783655986186034 1107 80-81 0.38374214418340813 1107 82-83 0.2893547315272116 1107 84-85 0.2789009420095354 1107 86-87 0.11593588809962085 1107 88-89 0.31028696628911945 1107 90-91 0.34619293309926036 1107 92-93 0.340559859401079 1107 94-95 0.1758088572818508 1107 96-97 0.09873527941265081 1107 98-99 0.13355461360685794 1107 100-101 0.2466765735919516 1108 1 0.18951522089390238 1108 2 0.2836781689187191 1108 3 0.2290553963744415 1108 4 0.18673974149211148 1108 5 0.0788591960311038 1108 6 0.29359444270224344 1108 7 0.25593097190606784 1108 8 0.18960824881346383 1108 9 0.2211026840314929 1108 10-11 0.31797851370742336 1108 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273.0 10 381.0 11 543.0 12 897.0 13 1414.0 14 1908.0 15 2492.0 16 3202.0 17 4111.0 18 4961.0 19 6361.0 20 7404.0 21 9222.0 22 10482.0 23 10756.0 24 11111.0 25 11549.0 26 12603.0 27 13905.0 28 16153.0 29 18992.0 30 22789.0 31 27510.0 32 34452.0 33 44347.0 34 61624.0 35 92261.0 36 157103.0 37 283748.0 38 366997.0 39 132093.0 40 146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.81580828290048 20.050940599569977 14.508111243255279 22.625139874274264 2 14.774257687938148 20.599072846034943 40.91240611596813 23.71426335005878 3 17.937432266243686 26.1174061513205 30.705993871464333 25.239167710971483 4 12.198418154045527 17.58191023933693 39.57989841530506 30.639773191312486 5 12.985099928100869 38.48561725111502 35.3330157301269 13.196267090657205 6 29.906549563932046 38.42480949870078 18.324128996072396 13.344511941294781 7 26.70378284116916 33.2654444228615 22.734216741127828 17.296555994841516 8 25.519696091160966 35.942390521689724 20.359394694448056 18.17851869270125 9 25.733199452304884 16.53192149703332 21.106490187129165 36.628388863532635 10-11 24.169064036242823 26.838671086736944 29.179730778865753 19.812534098154483 12-13 24.949119643870368 24.26801296052473 29.028384191781882 21.754483203823018 14-15 22.260520840104263 26.0900255694703 26.508843414199855 25.140610176225582 16-17 21.861408384945907 29.268049810693103 26.97262162694505 21.89792017741594 18-19 21.825844437558104 28.52990393554385 28.63555660876064 21.00869501813741 20-21 22.7229973035842 27.386349846010205 28.569398177049454 21.321254673356137 22-23 22.48939695089908 27.22441729694587 28.298898478219197 21.987287273935852 24-25 22.391147009754295 27.37809682283888 28.210342336905462 22.020413830501358 26-27 22.29369881145268 27.368220808725297 28.384284091016767 21.953796288805265 28-29 22.193225856060923 27.503970449216514 28.31362139224831 21.98918230247425 30-31 22.44850806589743 27.410713179413282 28.10505892445469 22.035719830234598 32-33 22.187467884732705 27.529954205906513 28.14842592369886 22.134151985661923 34-35 22.45087685157043 27.390633165477535 28.198571294253473 21.95991868869856 36-37 22.582763549271835 27.248323810814927 28.10473093874612 22.06418170116712 38-39 22.441766137443505 27.55156481981558 28.050103096841063 21.95656594589985 40-41 22.37766315284644 27.491980749425476 28.060780853797816 22.06957524393026 42-43 22.37671563857724 27.45743292145617 27.842998343307745 22.322853096658847 44-45 22.586657489282207 27.445476737994557 27.723900383530488 22.243965389192756 46-47 22.52593281626568 27.38953878427381 27.771916119487255 22.31261227997325 48-49 22.442778328529755 27.557404039674566 27.705049337894 22.29476829390168 50-51 22.243619079222054 27.696450678340334 27.591394987155198 22.46853525528241 52-53 22.518256054474957 27.65416628982708 27.38955196447691 22.438025691221046 54-55 22.456519527051498 27.637741200052663 27.498565756202993 22.407173516692847 56-57 22.525690657017552 27.65094344097014 27.271297789073017 22.552068112939292 58-59 22.74744426927586 27.360927613710228 27.533607685758742 22.358020431255166 60-61 22.68413968880513 27.516798362716177 27.55405912381375 22.245002824664944 62-63 22.358412780870196 27.693517175974787 27.620672762363185 22.327397280791832 64-65 22.425440902835884 27.749081045693913 27.42357434817466 22.401903703295538 66-67 22.602028364711664 27.608333944374465 27.28840375263158 22.50123393828229 68-69 22.4486616776374 27.565949346261565 27.39802289680604 22.587366079294995 70-71 22.479509519670533 27.30611141729454 27.570114081394376 22.644264981640543 72-73 22.424692869003028 27.393017905079773 27.533021573839626 22.649267652077572 74-75 22.268967057542373 27.686729154228274 27.369084860366428 22.67521892786292 76-77 22.33917445198425 27.419815489799348 27.539863991293107 22.701146066923297 78-79 22.367962012530143 27.473769027308236 27.35713826948566 22.80113069067596 80-81 22.447405278438353 27.452219774448157 27.29906773849992 22.801307208613572 82-83 22.46260912700222 27.30047106755251 27.564837898415 22.672081907030268 84-85 22.40159862899616 27.495441113116208 27.372946676982757 22.730013580904878 86-87 22.103199002786976 27.551109720420037 27.461359089321025 22.884332187471966 88-89 22.419070373984333 27.25232315310468 27.355189363196146 22.973417109714838 90-91 22.37010703163081 27.199446015720547 27.499022208990425 22.931424743658223 92-93 22.529829956109225 27.169278795471335 27.50400798969749 22.79688325872194 94-95 22.225688822622086 27.241713639102084 27.556189031165466 22.976408507110364 96-97 22.109044645648122 27.173731065421023 27.662567738457426 23.054656550473425 98-99 22.22230326893402 27.21635302197051 27.523256657875933 23.038087051219534 100-101 22.267835830392738 27.125113816243605 27.35312918549322 23.253921167870438 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 47.0 1 33.0 2 37.0 3 89.0 4 93.5 5 74.5 6 117.5 7 209.0 8 349.0 9 458.5 10 479.0 11 475.5 12 484.0 13 533.0 14 625.0 15 712.0 16 791.5 17 855.0 18 963.0 19 1077.0 20 1190.5 21 1418.5 22 1707.0 23 2053.0 24 2554.5 25 3217.5 26 4176.0 27 5644.5 28 7701.0 29 10942.0 30 15815.5 31 21617.5 32 28626.0 33 35245.0 34 41173.5 35 45480.0 36 48654.0 37 50448.5 38 48346.5 39 46112.0 40 44119.0 41 41806.0 42 40677.5 43 41301.5 44 42696.5 45 43664.0 46 44169.0 47 46054.0 48 49503.0 49 49859.5 50 48161.0 51 46863.5 52 45435.5 53 44160.0 54 42599.0 55 40383.5 56 37584.5 57 34551.5 58 31327.0 59 27131.5 60 22609.0 61 18684.0 62 14964.0 63 11350.5 64 8270.0 65 6242.0 66 4714.5 67 3346.0 68 2341.0 69 1744.0 70 1368.5 71 1010.0 72 677.5 73 514.5 74 402.0 75 283.0 76 208.0 77 150.0 78 110.0 79 85.0 80 58.0 81 41.0 82 36.0 83 28.5 84 19.5 85 21.5 86 19.5 87 12.5 88 11.0 89 9.5 90 8.5 91 6.5 92 2.5 93 3.5 94 4.5 95 2.0 96 1.5 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47368424888721733 2 0.3667609078935956 3 0.19526082516831134 4 0.22623725319986504 5 0.24977933850384582 6 0.19890511081908235 7 0.19074191096135526 8 0.1933657966299104 9 0.19212673950864825 10-11 0.20422576786920804 12-13 0.19110633952643236 14-15 0.2040799964431772 16-17 0.08899345559182834 18-19 0.03906674217626535 20-21 3.2798570856939195E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.14405861177497847 46-47 0.21461198197390546 48-49 0.08440165567185685 50-51 0.06075024179835293 52-53 0.06989739878178819 54-55 0.07868012720014635 56-57 0.11096849806597761 58-59 0.0924190841035531 60-61 0.14117962611086937 62-63 0.24347472432801193 64-65 0.13407326909186587 66-67 0.10200355536508089 68-69 0.10717844098917574 70-71 0.08684332705787344 72-73 0.07110001304654262 74-75 0.14030499755468434 76-77 0.21727231049896828 78-79 0.1366607119039133 80-81 0.08389145568074892 82-83 0.08662466991882718 84-85 0.0974117554451094 86-87 0.07215685588526623 88-89 0.09464209835052342 90-91 0.11479499799928716 92-93 0.16377419714564972 94-95 0.1306476405801411 96-97 0.08786372704008934 98-99 0.07722241293983795 100-101 0.06060447037232209 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1372011.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.711419195847256 #Duplication Level Percentage of deduplicated Percentage of total 1 69.21402426684894 37.17583471628267 2 15.743599889601622 16.912221866441744 3 6.288866740894545 10.13351873361026 4 3.1384413635847888 6.742805588043561 5 1.7071092672627617 4.584563073353292 6 1.0468051127751796 3.3735232937174295 7 0.6951714025070421 2.613704982911456 8 0.48453213979744836 2.081992709961729 9 0.3362708992416447 1.6255428509279242 >10 1.2883861556619092 11.109611696893737 >50 0.037821674338536594 1.3684789088924911 >100 0.01774277113187585 1.5749854926663722 >500 6.823979742932306E-4 0.219402246042785 >1k 5.459183794345845E-4 0.4838138402547213 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2320 0.16909485419577538 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1897 0.13826419759025257 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4577142603084087E-4 2 0.0 0.0 0.0 0.0 1.4577142603084087E-4 3 0.0 0.0 0.0 0.0 1.4577142603084087E-4 4 0.0 0.0 0.0 7.288571301542043E-5 1.4577142603084087E-4 5 0.0 0.0 0.0 7.288571301542043E-5 1.4577142603084087E-4 6 0.0 0.0 0.0 7.288571301542043E-5 1.4577142603084087E-4 7 0.0 0.0 0.0 7.288571301542043E-5 2.186571390462613E-4 8 0.0 0.0 0.0 1.4577142603084087E-4 2.186571390462613E-4 9 0.0 0.0 0.0 1.4577142603084087E-4 2.186571390462613E-4 10-11 0.0 3.6442856507710216E-5 0.0 2.186571390462613E-4 2.186571390462613E-4 12-13 0.0 7.288571301542043E-5 0.0 2.9154285206168173E-4 2.9154285206168173E-4 14-15 0.0 7.288571301542043E-5 0.0 8.017428431696247E-4 2.9154285206168173E-4 16-17 0.0 7.288571301542043E-5 0.0 0.002478114242524295 2.9154285206168173E-4 18-19 0.0 7.288571301542043E-5 0.0 0.0035349570812478913 3.6442856507710216E-4 20-21 0.0 7.288571301542043E-5 0.0 0.004409585637432936 5.10199991107943E-4 22-23 0.0 7.288571301542043E-5 0.0 0.007616557010111435 5.10199991107943E-4 24-25 0.0 7.288571301542043E-5 0.0 0.01162527122595956 5.10199991107943E-4 26-27 0.0 7.288571301542043E-5 0.0 0.013119428342775678 5.10199991107943E-4 28-29 0.0 7.288571301542043E-5 0.0 0.015342442589746002 5.10199991107943E-4 30-31 0.0 7.288571301542043E-5 0.0 0.019168942523055572 5.10199991107943E-4 32-33 0.0 7.288571301542043E-5 0.0 0.026311742398566776 5.10199991107943E-4 34-35 0.0 7.288571301542043E-5 0.0 0.03429272797375531 5.10199991107943E-4 36-37 0.0 7.288571301542043E-5 0.0 0.047302827747007864 5.10199991107943E-4 38-39 0.0 7.288571301542043E-5 0.0 0.07022538449035759 5.10199991107943E-4 40-41 0.0 7.288571301542043E-5 0.0 0.09941611255303347 5.10199991107943E-4 42-43 0.0 1.0932856952313065E-4 0.0 0.12539986924303084 5.10199991107943E-4 44-45 0.0 1.4577142603084087E-4 0.0 0.1569958258352156 5.466428476156532E-4 46-47 0.0 1.4577142603084087E-4 0.0 0.19773893941083565 5.830857041233635E-4 48-49 0.0 1.4577142603084087E-4 0.0 0.24383915289308905 5.830857041233635E-4 50-51 0.0 1.4577142603084087E-4 0.0 0.2919437234832665 5.830857041233635E-4 52-53 0.0 1.4577142603084087E-4 0.0 0.34351036544167646 5.830857041233635E-4 54-55 0.0 1.4577142603084087E-4 0.0 0.39915860732895003 5.830857041233635E-4 56-57 0.0 1.4577142603084087E-4 0.0 0.46923822039327673 6.559714171387839E-4 58-59 0.0 1.4577142603084087E-4 0.0 0.5483192190150079 6.559714171387839E-4 60-61 0.0 1.4577142603084087E-4 0.0 0.6334861746735267 6.559714171387839E-4 62-63 0.0 1.4577142603084087E-4 0.0 0.7226254016913858 7.288571301542043E-4 64-65 0.0 1.4577142603084087E-4 0.0 0.8169395143333399 7.288571301542043E-4 66-67 0.0 1.4577142603084087E-4 0.0 0.931880283758658 7.288571301542043E-4 68-69 0.0 1.4577142603084087E-4 0.0 1.0616532957826141 7.288571301542043E-4 70-71 0.0 1.4577142603084087E-4 0.0 1.2068416361093315 7.652999866619146E-4 72-73 0.0 1.4577142603084087E-4 0.0 1.3523215192881106 8.381856996773349E-4 74-75 0.0 1.4577142603084087E-4 0.0 1.5081511737150795 9.110714126927555E-4 76-77 0.0 1.4577142603084087E-4 0.0 1.6758976422200695 9.475142692004656E-4 78-79 0.0 1.4577142603084087E-4 0.0 1.8811073672149858 9.475142692004656E-4 80-81 0.0 1.4577142603084087E-4 0.0 2.119407205918903 9.475142692004656E-4 82-83 0.0 1.4577142603084087E-4 0.0 2.3731952586385967 9.475142692004656E-4 84-85 0.0 1.4577142603084087E-4 0.0 2.623448354277043 9.475142692004656E-4 86-87 0.0 1.8221428253855108E-4 0.0 2.8943645495553607 9.839571257081758E-4 88-89 0.0 2.186571390462613E-4 0.0 3.198297972829664 0.001020399982215886 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4240 0.0 24.1308 1 GTATCAA 5855 0.0 23.81441 1 GTACATG 4660 0.0 22.05804 1 TCAACGC 6325 0.0 21.416264 4 CAACGCA 6390 0.0 21.272795 5 GTATAGA 495 0.0 21.150303 1 ATCAACG 6405 0.0 21.147224 3 TACATGG 4725 0.0 21.137161 2 AACGCAG 6500 0.0 21.124441 6 GTATAGG 385 0.0 21.012962 1 TATCAAC 6730 0.0 20.422644 2 ACATGGG 4730 0.0 20.095392 3 AGAGTAC 5050 0.0 19.521788 10-11 GAGTACT 2655 0.0 19.41557 12-13 CGCAGAG 7310 0.0 18.71939 8 ACGCAGA 7325 0.0 18.679695 7 ACTCTCG 180 1.2308646E-4 18.477455 8 TCTATAC 430 3.6379788E-12 17.683945 3 CATGGGG 2700 0.0 17.603363 4 GTATATA 925 0.0 17.49187 1 >>END_MODULE