FastQCFastQC Report
Fri 10 Feb 2017
SRR2031663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372011
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25750.1876807110147076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23890.17412396839383942No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14860.10830816954091477No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG650.00584667629.2285045
GTATCAA58400.023.5196021
GGTATCA42350.022.1084231
GTACATG42450.021.8324181
TCAACGC63650.021.1185384
CCTAACG1353.7956453E-421.1117842
CAACGCA64650.020.7176515
AACGCAG65900.020.4688226
ATCAACG66000.020.2953623
TACATGG43900.020.1259252
TATCAAC67100.020.1049612
ACATGGG42600.019.5128213
AGAGTAC52000.019.18120610-11
GACCGTG2502.1745655E-718.9985287
ATAGCCC3801.0913936E-1118.7499143
ACGCAGA72900.018.5033687
CGCAGAG72900.018.3079098
GAGTACT31400.018.00019612-13
GTATAAA7550.017.6260911
CATGGGG26150.017.4371414
Produced by FastQC (version 0.11.5)