FastQCFastQC Report
Fri 10 Feb 2017
SRR2031662_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031662_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2267482
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT311751.3748730971183014No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC210510.9283866420990333No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT201010.8864899478805125No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT135620.5981083863069255No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA115310.5085376642460668No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC102960.4540719617619897No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC100180.4418116659801489No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC91510.4035754197828252No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT91090.4017231448805327No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC85740.37812869076799727No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA85020.3749533623640673No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT84510.37270417141128354No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC78710.3471251370462919No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT78640.34681642456257644No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT77200.3404657677547165No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT76270.33636430189964023No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA72210.31845897784414606No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA68490.3020531144238411No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC68290.30117107875608273No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT68240.30095056983914314No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC68090.30028904308832444No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC67820.29909829493685064No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC62270.2746218051565569No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA62010.2734751587884711No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA55900.246528969138454No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG55020.24264801220031737No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC54700.241236755131904No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT54660.24106034799835238No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT53840.2374440017605432No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT53590.2363414571758453No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA53580.2362973553924574No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG53430.2356358286416386No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG52940.2334748412556307No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA52900.233298434122079No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG52600.2319753806204415No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT52090.22972618966765776No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC52040.2295056807507182No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT50370.2221406829249361No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT50190.2213468508239536No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA49380.21777460636953236No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG48540.21407005656494735No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC48460.21371724229784406No Hit
ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC46920.20692556765610487No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA46330.2043235624362178No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT46020.20295640715119237No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT44530.19638524142639283No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC43780.19307760767229906No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC43750.1929453023221353No Hit
TAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTC43150.1902991953188603No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC43090.19003458461853281No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT42940.18937305786771408No Hit
ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT42900.1891966507341624No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC41710.1839485385110003No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA41700.1839044367276124No Hit
CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG40740.1796706655223724No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT40620.1791414441217174No Hit
AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA40230.17742147456958865No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG38850.17133542846205616No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC38210.1685129143252295No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA37450.16516117878774783No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC36570.16128022184961116No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA36370.1603981861818528No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT36120.1592956415971549No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA36050.15898692911343948No Hit
CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC35750.157663875611802No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC35240.15541468465901825No Hit
GTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTCAG35060.15462085255803573No Hit
TAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCA34690.15298908657268281No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG34620.1526803740889674No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG34540.15232755982186408No Hit
GTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAACAATTC34540.15232755982186408No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC33460.14756456721596908No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT33030.14566819053028868No Hit
CTACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTC32790.14460974772897867No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT32650.14399232276154783No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA32020.14121391040810907No Hit
GAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTTAGGCTTTATT31920.14077289257422992No Hit
TTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGT31670.139670347989532No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG31640.13953804263936825No Hit
ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT31050.13693603741948115No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC30950.136495019585602No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG30740.13556888213445575No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC30620.13503966073380075No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC30520.1345986428999216No Hit
ATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGC30500.13451043933314577No Hit
CTATAATTATCGCAATTCCTACCGGTGTCAAAGTATTTAGCTGACTTGCA30410.1341135232826545No Hit
ACTTACTACTCCGGAAAAAAAGAACCTTTCGGCTATATAGGAATAGTATG30390.13402531971587867No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA30360.13389301436571494No Hit
CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA30090.13270226621424117No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT29900.13186433232987074No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA29720.13107050022888825No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA29560.13036487169468158No Hit
ACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAG29530.13023256634451782No Hit
GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA29410.12970334494386285No Hit
CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT29380.1295710395936991No Hit
GCTATACTATGAGCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGTCT29150.128556698575777No Hit
TCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAA29050.12811568074189783No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA28640.12630750762299325No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG28160.12419062202037325No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC27740.12233834711808077No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC27670.12202963463436535No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC27150.11973634189819367No Hit
GATTAGATGTAGACACACGAGCTTACTTTACATCAGCCACTATAATTATC27040.1192512222809266No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT27000.11907481514737493No Hit
GGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATTGTATG26620.1173989473786341No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT26000.11466463680858327No Hit
GATGTAGACACACGAGCTTACTTTACATCAGCCACTATAATTATCGCAAT25960.1144882296750316No Hit
ACAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGT25910.11426772075809202No Hit
ACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCAT25880.11413541540792825No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT25840.1139590082743766No Hit
ATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATG25730.11347388865710951No Hit
ATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGCTTCA25560.11272415833951495No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC25500.11245954763918742No Hit
TCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTA25310.11162161375481701No Hit
GTGTAGGGTTGCAAGTCAGCTAAATACTTTGACACCGGTAGGAATTGCGA25230.11126879948771369No Hit
GTCAAGGGATGAGTTGGATAAAACAATTCCGGTTAGACCACCAACTGTAA25120.11078367987044659No Hit
GTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAA25100.11069547630367077No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA25040.11043086560334327No Hit
ACTTTACATCAGCCACTATAATTATCGCAATTCCTACCGGTGTCAAAGTA24850.10959293171897286No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC24780.10928421923525743No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT24370.10747604611635285No Hit
TATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCT24180.10663811223198244No Hit
GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC24080.10619709439810326No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC23870.10527095694695703No Hit
TCAGAATACTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGTTC23600.10408020879548326No Hit
ACATAAATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACC23500.10363919096160411No Hit
CTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTC23330.10288946064400953No Hit
GCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGT22890.1009489821749412No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGAG156.594935E-495.0862353
CATGGAT603.274181E-1063.3936234
GACATAT11500.061.3174481
ATGTATT701.8189894E-1161.2582631
GGTCGGG250.004961550757.1743771
GTCGGGA250.004981657.1161542
ACCGTGT250.00501134957.0303088
CGCTCTT250.00501441957.0214847
GGACCAC352.7812115E-454.3145759
TACCTGG1850.054.0287972
ACATATA13300.052.9648552
TAACTTT13000.052.6363756
ACCTGGG1850.051.3979643
AAGACAT3650.049.4991385
CTTCGAT1151.8189894E-1245.4760253
TCTTCGA1201.8189894E-1243.6303982
TTCGATA1201.8189894E-1243.583124
ATTGAAC4700.043.4805538
GGACAAC1650.043.2047738
AACTTTA16200.042.824777
Produced by FastQC (version 0.11.5)