FastQCFastQC Report
Fri 10 Feb 2017
SRR2031662_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031662_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2267482
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT342791.5117650327543946No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC224010.9879240496727207No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT181950.8024319487431433No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT126460.5577111527235938No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA115720.5103458373649714No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT96100.4238181383578789No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC95760.4223186777226898No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC95360.42055460638717307No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC94020.41464496741319223No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC93040.4103229926411764No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA89790.3959899130401035No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC82400.36339869511643313No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT81780.3606643845463823No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT81070.35753315792584023No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT78960.3482276816309898No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT78550.3464195085120852No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT75750.33407100916346855No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC74950.3305428664924352No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC74500.32855828623997896No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA74140.32697062203801397No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT73980.3262649935038073No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA72550.31995843847933525No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT68510.3021413179906169No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC65160.2873672205556648No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA64370.2838831796680194No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC60100.265051718161379No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA59970.2644783949773361No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG58310.25715749893494194No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA58180.25658417575089904No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC57150.2520416920619436No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT56330.24842534582413442No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG55620.24529411920359234No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG54970.24242750328337778No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC51930.22902056113345112No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG51530.22725648979793445No Hit
ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC50920.22456628101127155No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA50860.22430167031094406No Hit
ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT50110.2209940365568503No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT49800.21962688127182486No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC48220.21265879949653402No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC48170.21243829057959446No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT47260.20842502829129406No Hit
CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC46510.2051173945372003No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG46470.20494098740364863No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC46000.20286820358441654No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT45310.1998251805306503No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA45000.19845802524562486No Hit
AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA44960.19828161811207323No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT44220.19501808614136737No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA43950.19382733798989366No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA43750.1929453023221353No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT43090.19003458461853281No Hit
CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG42790.1887115311168953No Hit
TAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTC42600.1878735972325249No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT41870.18465416704520696No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT41070.18112602437417366No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC40860.18019988692302738No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC40490.1785681209376745No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG40030.1765394389018303No Hit
TAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCA39430.1738933318985553No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA39250.1730994997975728No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC37890.16710165725681614No Hit
CTACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTC37570.1656904001884028No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC36860.16255917356786076No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT35860.15814899522906906No Hit
TTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGT35770.1577520791785778No Hit
ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT35460.1563849238935524No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG34900.1539152240238291No Hit
ATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGC34140.15056348848634743No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA34040.15012247065246825No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT33610.14822609396678782No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG32740.14438923881203908No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA32440.14306618531040158No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG31860.14050828187390244No Hit
ACTTACTACTCCGGAAAAAAAGAACCTTTCGGCTATATAGGAATAGTATG31800.14024367117357492No Hit
GTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTCAG31660.13962624620614408No Hit
GAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTTAGGCTTTATT31330.13817088735434283No Hit
GTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAACAATTC31240.13777397130385158No Hit
CTATAATTATCGCAATTCCTACCGGTGTCAAAGTATTTAGCTGACTTGCA31230.13772986952046365No Hit
TCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAA31220.13768576773707575No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC31210.13764166595368782No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT31070.137024240986257No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG30890.1362304088852745No Hit
ACAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGT30740.13556888213445575No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC30740.13556888213445575No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG30540.1346868464666974No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA30340.1338048107989391No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA30140.13292277513118075No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA29970.13217304481358616No Hit
GATTAGATGTAGACACACGAGCTTACTTTACATCAGCCACTATAATTATC29580.13045307526145744No Hit
GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA29150.128556698575777No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC28760.12683672902364826No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC28750.12679262724026036No Hit
GGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATTGTATG28740.12674852545687243No Hit
ATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGCTTCA28690.12652801653993284No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC28440.12542547195523493No Hit
ATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATG28440.12542547195523493No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA28380.1251608612549074No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT28280.12471984342102826No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC28010.12352909526955451No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA27680.12207373641775326No Hit
TCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTA27610.12176502393403785No Hit
GCTATACTATGAGCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGTCT27300.12039786864901243No Hit
CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA27080.11942762941447828No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT26980.1189866115805991No Hit
CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT26910.11867789909688368No Hit
ACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAG26630.11744304916202201No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC26100.11510565464246245No Hit
GATGTAGACACACGAGCTTACTTTACATCAGCCACTATAATTATCGCAAT26000.11466463680858327No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT25920.11431182254147992No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT25830.11391490649098868No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC25550.11268005655612702No Hit
ACTTTACATCAGCCACTATAATTATCGCAATTCCTACCGGTGTCAAAGTA25420.1121067333720841No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA24840.10954882993558493No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT24760.10919601566848161No Hit
GTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAA24550.10826987821733534No Hit
ACATAAATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACC24530.10818167465055951No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC24300.10716733363263743No Hit
TGATAGAACATAGTGGAAATGGGCTACTACATAGTATGTATCGTGAAGCA24020.10593248369777576No Hit
ACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCAT23970.10571197478083619No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC23860.1052268551635691No Hit
GTCAAGGGATGAGTTGGATAAAACAATTCCGGTTAGACCACCAACTGTAA23680.1044330230625866No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC23410.10324227491111285No Hit
GTAGTAGGCAAACAATAAGGAATGTTGATCCAATAATTACATGGAGTCCA23340.10293356242739744No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT23230.10244844281013035No Hit
ATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAG23180.10222793389319078No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT23090.10183101784269953No Hit
TTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTAT23080.1017869160593116No Hit
TATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGCT23050.10165461070914786No Hit
GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC23030.10156640714237201No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA22780.1004638625576741No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG200.002074998871.244285
ACGCACC353.4197164E-667.851697
CGCACCT353.4197164E-667.851698
GACATAT14150.057.094031
AACCGTG250.005023500456.9954267
AAGACAT4150.056.079835
TACCTGG1650.054.698612
TAACTTT12500.052.8157586
AGACGCA451.5171632E-552.773545
CTAGACG558.1926373E-751.8197333
ACATATA15950.051.2241022
ACCTGGG1600.050.470263
ATGGGCG405.391431E-447.4961855
CTCCCGC2650.046.6000337
TACTCCC2750.044.9054835
ACTCCCG2750.044.9054836
AACTTTA15000.044.6464167
ACTTAAT56950.043.3918151
AAAGACA5500.043.1831134
GTCAAGC554.967201E-543.178359
Produced by FastQC (version 0.11.5)