FastQCFastQC Report
Fri 10 Feb 2017
SRR2031661_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031661_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693173
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32000.032.7778931
TCAACGC50050.031.4437074
GTATCAA54000.031.254881
ATCAACG51150.030.9522533
CAACGCA50900.030.9186155
AACGCAG53750.029.2683126
ACGCAGA57400.027.3233537
CGCAGAG57850.027.1108138
TATCAAC61800.025.7320732
GCAGAGT69700.021.9560939
GTACATG64550.021.6411191
ACATGGG62000.020.0143973
CAGTGCG2401.4067882E-719.8025329
TACATGG69350.018.8800432
AGAGTAC62100.018.48150410-11
TCTTACG2403.02445E-617.8545132
CATGGGG24750.017.6734964
GGATCGA1700.001781406216.7745346
CAGAGTA71600.016.42759310-11
TACACTG31550.016.4261955
Produced by FastQC (version 0.11.5)