FastQCFastQC Report
Fri 10 Feb 2017
SRR2031661_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031661_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693173
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC43850.032.389334
GGTATCA29650.032.2161331
ATCAACG45200.031.7378123
CAACGCA45750.030.9363545
GTATCAA48250.030.5327851
AACGCAG48200.029.5609346
ATCGCGA350.00839895127.13975566-67
ACGCAGA53150.026.8078487
CGCAGAG52650.026.7016018
CATCGCG458.957827E-426.38587266-67
TATCAAC58200.024.6490692
ATACGAC1252.2564728E-422.8007813
GTACATG59050.021.4878711
GCAGAGT64650.021.30469
ACATGGG56850.020.7219053
CATGGGG24100.019.1185554
TACATGG64850.019.1182022
GTAGGAC11700.018.2698553
CTCGCGC800.001192703417.81046360-61
TAATACT26200.017.7674544
Produced by FastQC (version 0.11.5)