FastQCFastQC Report
Fri 10 Feb 2017
SRR2031660_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031660_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920141
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG63550.028.411451
GTACCGT2550.027.949536
GTATCAA48050.025.5797351
TACATGG66550.025.5264322
ACATGGG62000.025.4537163
TACCGTA2452.9649527E-1023.2722597
TCAACGC52000.023.0375354
AACGCAG53150.022.7960876
CAACGCA52800.022.7785155
GGTATCA36500.022.710471
ATCAACG54200.022.10073
CATGGGG33300.021.9844344
CCGTATG2606.2391337E-1021.93029
TATCAAC59450.020.3306772
ACCGTAT2401.4095713E-719.79818
ACGCAGA60850.019.7552877
CGCAGAG61400.019.501458
GAGTACA48700.018.8798311
CGTACAC2905.1757524E-818.0301573
ACCGTAA1350.00938504617.598318
Produced by FastQC (version 0.11.5)