FastQCFastQC Report
Fri 10 Feb 2017
SRR2031660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920141
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG63150.026.415351
ACATGGG60600.025.4725933
TACATGG67000.023.891322
GTATCAA53050.023.740131
GGTATCA39650.023.0133931
TCAACGC55350.022.4825784
CAACGCA56500.022.107885
ATCAACG57450.021.6607593
AACGCAG58200.021.4621166
GTCTACG1353.7837276E-421.1222441
CATGGGG32550.020.8663964
CGTAGCG704.8462834E-420.36230942-43
TATCAAC63000.019.753582
GCACCGT3153.2196112E-1019.6007466
CATGGGA30250.018.9986364
TGCACCG3003.7252903E-918.9976445
ACGCAGA65500.018.9251377
CGCAGAG66700.018.3710378
TAGGTCG2854.2826287E-818.3310625
ATGGGAG14600.017.5663175
Produced by FastQC (version 0.11.5)