##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031660_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1920141 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.495823483796244 34.0 31.0 34.0 31.0 34.0 2 32.70954060144541 34.0 31.0 34.0 31.0 34.0 3 32.82754599792411 34.0 31.0 34.0 31.0 34.0 4 36.265515397046364 37.0 37.0 37.0 35.0 37.0 5 35.94154543859019 37.0 35.0 37.0 35.0 37.0 6 35.99847407039379 37.0 37.0 37.0 35.0 37.0 7 35.96860022258782 37.0 36.0 37.0 35.0 37.0 8 36.0873696254598 37.0 37.0 37.0 35.0 37.0 9 37.99215942995853 39.0 39.0 39.0 35.0 39.0 10-11 37.969091332355276 39.0 38.5 39.0 35.0 39.0 12-13 37.98444489232822 39.0 38.5 39.0 35.0 39.0 14-15 39.467944801970276 41.0 39.0 41.0 36.5 41.0 16-17 39.441629286599266 41.0 39.0 41.0 36.0 41.0 18-19 39.43914301085181 41.0 39.0 41.0 36.5 41.0 20-21 39.45673234413515 41.0 39.0 41.0 36.0 41.0 22-23 39.400313049927064 41.0 39.0 41.0 36.0 41.0 24-25 39.33726976300178 41.0 39.0 41.0 36.0 41.0 26-27 39.24398572813142 41.0 39.0 41.0 36.0 41.0 28-29 39.15971066708122 41.0 39.0 41.0 36.0 41.0 30-31 39.07864344337213 40.5 39.0 41.0 36.0 41.0 32-33 38.95587381343349 40.0 38.5 41.0 35.0 41.0 34-35 38.84944152538798 40.0 38.0 41.0 35.0 41.0 36-37 38.72351848119487 40.0 38.0 41.0 35.0 41.0 38-39 38.61941623037058 40.0 38.0 41.0 35.0 41.0 40-41 38.51489864546406 40.0 38.0 41.0 34.0 41.0 42-43 38.390168222021195 40.0 38.0 41.0 34.0 41.0 44-45 38.341872289587066 40.0 38.0 41.0 34.0 41.0 46-47 38.38151000369244 40.0 38.0 41.0 34.0 41.0 48-49 38.497489767678516 40.0 38.0 41.0 34.0 41.0 50-51 38.42251792967287 40.0 38.0 41.0 34.0 41.0 52-53 38.32772332865139 40.0 38.0 41.0 34.0 41.0 54-55 38.22621047100187 40.0 38.0 41.0 34.0 41.0 56-57 38.11756298105191 40.0 37.0 41.0 34.0 41.0 58-59 37.92597028030754 40.0 37.0 41.0 33.0 41.0 60-61 37.65539015103579 40.0 36.5 41.0 33.0 41.0 62-63 37.46657094452959 39.0 36.0 41.0 33.0 41.0 64-65 37.17964435945069 39.0 35.5 41.0 32.0 41.0 66-67 36.90372477854491 39.0 35.0 41.0 32.0 41.0 68-69 36.62968005995393 38.0 35.0 40.5 32.0 41.0 70-71 36.30987073345135 37.0 35.0 40.0 31.5 41.0 72-73 35.96084245896525 37.0 35.0 39.5 31.0 41.0 74-75 35.61729164681135 36.0 35.0 39.0 31.0 41.0 76-77 34.9934939673701 36.0 34.0 39.0 30.5 40.0 78-79 34.94746948270986 35.0 34.5 37.5 31.0 39.5 80-81 34.6718209756471 35.0 34.0 37.0 30.5 39.0 82-83 34.36665171984765 35.0 34.0 37.0 30.0 39.0 84-85 34.07653838962868 35.0 34.0 36.0 30.0 38.0 86-87 33.8190619855521 35.0 34.0 36.0 30.0 37.0 88-89 33.5754259713219 35.0 34.0 36.0 29.5 37.0 90-91 33.40432759885863 35.0 34.0 35.0 29.0 36.0 92-93 33.26812249725411 35.0 34.0 35.0 29.0 36.0 94-95 33.134500018488225 35.0 34.0 35.0 29.0 36.0 96-97 33.034419607726726 35.0 34.0 35.0 29.0 36.0 98-99 32.943296091276636 35.0 34.0 35.0 29.0 35.5 100-101 32.52242778004324 34.5 33.0 35.0 27.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08622232321994971 1101 2 0.16689271462452382 1101 3 0.14228463669079616 1101 4 0.06605851139772767 1101 5 0.18042364910055397 1101 6 0.09387417243587493 1101 7 0.24466317089852652 1101 8 0.10380322054380287 1101 9 0.14979789045131042 1101 10-11 0.03908834904286351 1101 12-13 0.08141796413126912 1101 14-15 0.10315068289354912 1101 16-17 0.10683799843075548 1101 18-19 0.07015690792822937 1101 20-21 0.0031083406131031666 1101 22-23 0.1724877838551251 1101 24-25 0.1520659393864534 1101 26-27 0.10533236902163168 1101 28-29 0.04726938527269908 1101 30-31 0.03625518866310529 1101 32-33 -0.08241692334288331 1101 34-35 -0.007704762173865731 1101 36-37 0.016667035563969534 1101 38-39 -0.011118019368723253 1101 40-41 0.009126926368864474 1101 42-43 -0.09534675481014432 1101 44-45 -0.10703948238123928 1101 46-47 7.815796056505064E-4 1101 48-49 0.039844582161656206 1101 50-51 0.08794482922350255 1101 52-53 -0.10509139106638798 1101 54-55 0.010251834349254807 1101 56-57 0.07134513215679306 1101 58-59 -0.03647967515967565 1101 60-61 -0.10930286994428684 1101 62-63 -0.09158662755298508 1101 64-65 -0.021530121767611377 1101 66-67 0.0538633370988677 1101 68-69 -0.10814149206503743 1101 70-71 -0.03622450176184344 1101 72-73 0.01529394570934528 1101 74-75 -0.05373211310686088 1101 76-77 -0.15575859123352132 1101 78-79 0.01877598604121289 1101 80-81 0.09525985575616858 1101 82-83 -0.03975528870470413 1101 84-85 -0.014923889472264307 1101 86-87 -0.0011569607088901535 1101 88-89 -0.03740509973614081 1101 90-91 0.01756538923105211 1101 92-93 -0.2595151457806324 1101 94-95 -0.055212701822775045 1101 96-97 2.2098979642493077E-4 1101 98-99 -0.1437408831731659 1101 100-101 -0.12665985298824012 1102 1 0.044629031815333065 1102 2 0.03942940225554992 1102 3 0.11398873971565848 1102 4 0.11431247965169433 1102 5 -0.008387372163291218 1102 6 0.08332610415195774 1102 7 0.13001207775686652 1102 8 0.002467491283546508 1102 9 0.18526359874720555 1102 10-11 0.16608445566161123 1102 12-13 -0.012927648327547558 1102 14-15 0.11079668469049153 1102 16-17 0.10619708797852212 1102 18-19 0.11872833649965742 1102 20-21 0.18107180272750867 1102 22-23 0.125545585592171 1102 24-25 0.11907103073116332 1102 26-27 0.04886935614352694 1102 28-29 -0.2220088417413777 1102 30-31 -0.19417907309190952 1102 32-33 -0.027469334244351273 1102 34-35 -0.031888649565296134 1102 36-37 0.034088419211762755 1102 38-39 0.08427581111944704 1102 40-41 0.13105564754286547 1102 42-43 0.17878022931683546 1102 44-45 0.16324503394100986 1102 46-47 0.28138355624536615 1102 48-49 0.1789191553871845 1102 50-51 0.2860400673187371 1102 52-53 0.07991609620524542 1102 54-55 -0.06802309826530717 1102 56-57 -0.061724649214880856 1102 58-59 0.031106428464163116 1102 60-61 0.17401249393711282 1102 62-63 0.06095604989535275 1102 64-65 0.2122614325899761 1102 66-67 0.13170101304975645 1102 68-69 0.04617417130723567 1102 70-71 -0.02621851194453484 1102 72-73 0.06884590737451646 1102 74-75 0.07825650624025116 1102 76-77 0.1974220017583903 1102 78-79 -0.05498262432117684 1102 80-81 0.015849852761263605 1102 82-83 -0.174373437851159 1102 84-85 -0.045166477073344424 1102 86-87 -0.15432856897484015 1102 88-89 0.006164831380957025 1102 90-91 0.0572299594616581 1102 92-93 0.15220692669615232 1102 94-95 0.17471242546083943 1102 96-97 0.2152105076161277 1102 98-99 0.02387217462857194 1102 100-101 0.11158213563110309 1103 1 0.04548783722611205 1103 2 0.1224884792673322 1103 3 0.1447289662521385 1103 4 0.07559112862636397 1103 5 0.004171518976292532 1103 6 -0.010348930556830283 1103 7 0.1534486649916218 1103 8 0.07528387231396039 1103 9 0.16461130113926714 1103 10-11 0.19398083760241747 1103 12-13 0.1837384114095002 1103 14-15 0.26376760517512565 1103 16-17 0.3140723791150819 1103 18-19 0.22168370441280416 1103 20-21 0.07173874251250112 1103 22-23 -0.025681170900078598 1103 24-25 0.1276052198352602 1103 26-27 0.14727351057676685 1103 28-29 0.15088226749836764 1103 30-31 0.17755355923771532 1103 32-33 0.14771710694796525 1103 34-35 0.10059649962558836 1103 36-37 0.11088094790815717 1103 38-39 0.22147642411835733 1103 40-41 0.1600490547698854 1103 42-43 0.1628373262207461 1103 44-45 0.09319966595199958 1103 46-47 0.21574108331063968 1103 48-49 -0.027458536914672038 1103 50-51 0.15779318352299754 1103 52-53 0.19918838759215163 1103 54-55 0.1533984826346213 1103 56-57 0.010540184524337803 1103 58-59 0.12746636807467127 1103 60-61 0.06247552054338712 1103 62-63 -0.08539213045378347 1103 64-65 0.015199730607598383 1103 66-67 -0.22738965464869665 1103 68-69 -0.14692162494649352 1103 70-71 -0.10538451478193167 1103 72-73 -0.0555443180239692 1103 74-75 -0.14966946351947286 1103 76-77 -0.11859562954703762 1103 78-79 0.06339103189871054 1103 80-81 0.010911721649293327 1103 82-83 -0.016335793041541535 1103 84-85 -0.014184616682321405 1103 86-87 -0.10620549882136032 1103 88-89 0.025157176481137355 1103 90-91 0.02307537448771768 1103 92-93 -0.016613581455672488 1103 94-95 0.0137747950658067 1103 96-97 -0.1242998519138041 1103 98-99 -0.16885567054368522 1103 100-101 -0.1491449660523685 1104 1 0.26497590583675645 1104 2 0.20479180147381726 1104 3 0.14471919739770556 1104 4 0.08923749464869957 1104 5 0.24128043012530753 1104 6 0.31051198772996 1104 7 0.29552652203782515 1104 8 0.23088218570315178 1104 9 0.2853677288645784 1104 10-11 0.18513875800868362 1104 12-13 0.1625466907668951 1104 14-15 0.15845790716297614 1104 16-17 0.26621602758682883 1104 18-19 0.2427835254618671 1104 20-21 0.07885300120479144 1104 22-23 0.12956817730024994 1104 24-25 0.07268200664918822 1104 26-27 0.14556794095486936 1104 28-29 0.015834910262938706 1104 30-31 0.0454563960406702 1104 32-33 0.1259707324592938 1104 34-35 0.12996294238046602 1104 36-37 0.13971842528601996 1104 38-39 0.14050003797218835 1104 40-41 0.1876205609752759 1104 42-43 0.10410516432984451 1104 44-45 -0.007790570258897844 1104 46-47 0.2590882039950628 1104 48-49 0.23245920209481596 1104 50-51 0.29891749183383354 1104 52-53 0.24457448528215053 1104 54-55 0.25660027894604553 1104 56-57 0.2468321299953118 1104 58-59 0.2037120960099017 1104 60-61 0.312827561112357 1104 62-63 0.2838759942083726 1104 64-65 0.09030865333451032 1104 66-67 0.06858420244960683 1104 68-69 0.14441980519931974 1104 70-71 0.203594582040445 1104 72-73 0.04429138431685686 1104 74-75 0.009227104573412248 1104 76-77 0.11661721931864832 1104 78-79 0.21632009626677728 1104 80-81 0.3425865808873283 1104 82-83 0.3687652928932579 1104 84-85 0.308276041970025 1104 86-87 0.2987853365459401 1104 88-89 0.25833213376953523 1104 90-91 0.06884046754872486 1104 92-93 0.27330648037033 1104 94-95 0.12908834273022052 1104 96-97 0.22868196426819765 1104 98-99 0.3420794765644146 1104 100-101 0.42043225240021087 1105 1 0.03531276842979736 1105 2 0.04854853807139392 1105 3 -0.019537388713317227 1105 4 0.10294623395709124 1105 5 0.08765707902055198 1105 6 -0.03053564660081065 1105 7 0.11166260595766175 1105 8 0.12804064149764116 1105 9 0.16067464222358296 1105 10-11 0.19700171262786625 1105 12-13 0.12917569562034714 1105 14-15 0.19441523923273252 1105 16-17 0.09684255440546252 1105 18-19 0.10684544327347822 1105 20-21 0.12427703924448963 1105 22-23 0.08279923028597835 1105 24-25 0.22919421741025303 1105 26-27 0.1487834287413463 1105 28-29 0.08104483039142707 1105 30-31 0.010070361881268752 1105 32-33 0.14072633472574836 1105 34-35 0.1167097950765168 1105 36-37 0.20732528278937679 1105 38-39 0.16691197716873063 1105 40-41 0.18328434440688568 1105 42-43 0.07302056609449181 1105 44-45 0.06013034613187074 1105 46-47 0.010186204831178713 1105 48-49 0.14474207273079287 1105 50-51 0.15054446839030078 1105 52-53 0.19611557775251498 1105 54-55 0.18813767984703844 1105 56-57 0.09323495046452024 1105 58-59 0.002388985201527305 1105 60-61 0.08791215383789819 1105 62-63 0.09303477094901069 1105 64-65 0.09763066769168205 1105 66-67 0.13443119378613488 1105 68-69 0.1021508192720475 1105 70-71 0.08164348431509438 1105 72-73 0.14306479840556818 1105 74-75 0.09079288097037619 1105 76-77 -0.051375014611139136 1105 78-79 0.08273986627374796 1105 80-81 0.012013085942236046 1105 82-83 -0.030691268148153483 1105 84-85 0.02230097521528762 1105 86-87 0.003888690157658914 1105 88-89 0.09599037709217129 1105 90-91 -3.3995583847001853E-4 1105 92-93 -0.10170565841890777 1105 94-95 -0.10421694799120473 1105 96-97 -0.1559149969365805 1105 98-99 -0.1366304645702101 1105 100-101 -0.009250926922597102 1106 1 0.20241742089320525 1106 2 0.15163826644734257 1106 3 0.16587588431340095 1106 4 0.07825424896860511 1106 5 0.22237178184376205 1106 6 0.06144522773369232 1106 7 0.16439832438508262 1106 8 0.12231297009498121 1106 9 0.13148473166782537 1106 10-11 0.2501157402158327 1106 12-13 0.20082783632950196 1106 14-15 0.2966429241349502 1106 16-17 0.2082557271497194 1106 18-19 0.2440922782689725 1106 20-21 0.2508486132805885 1106 22-23 0.23377415581339278 1106 24-25 0.23549401311687035 1106 26-27 0.27036498602513603 1106 28-29 0.29397888952979656 1106 30-31 0.2164757755914195 1106 32-33 0.25197526279622195 1106 34-35 0.09826939307240679 1106 36-37 0.2789925301514984 1106 38-39 0.31100501872295894 1106 40-41 0.36498234505742744 1106 42-43 0.35438679893237435 1106 44-45 0.2998755001104598 1106 46-47 0.2772525442513114 1106 48-49 0.36890873189770446 1106 50-51 0.4059915417494935 1106 52-53 0.3327459327395772 1106 54-55 0.39619968269351347 1106 56-57 0.3247762276259607 1106 58-59 0.2899653380826521 1106 60-61 0.4021928907339998 1106 62-63 0.24697413605816365 1106 64-65 0.35975873868138564 1106 66-67 0.320745080464377 1106 68-69 0.304507328568107 1106 70-71 0.24087178505448748 1106 72-73 0.3378265182755129 1106 74-75 0.4510033070037025 1106 76-77 0.3769423099556164 1106 78-79 0.48356597519849487 1106 80-81 0.4603303333686384 1106 82-83 0.4580221335743957 1106 84-85 0.5106608169747062 1106 86-87 0.3893976172253062 1106 88-89 0.4274801630662566 1106 90-91 0.43580229354858346 1106 92-93 0.39355085254852185 1106 94-95 0.4257533658286192 1106 96-97 0.3905283184016213 1106 98-99 0.4893686637437682 1106 100-101 0.4561249458883836 1107 1 0.05162844517704457 1107 2 0.09417639743966078 1107 3 0.09790871133873225 1107 4 0.1058526098035415 1107 5 0.12422835247141961 1107 6 0.07718891264866556 1107 7 0.12584831017477427 1107 8 0.11789198547855051 1107 9 0.10055848680843837 1107 10-11 0.12242185453471421 1107 12-13 0.016032501197003057 1107 14-15 0.12400286758521162 1107 16-17 0.16581761486156665 1107 18-19 0.021982132595105952 1107 20-21 -0.010277328911399763 1107 22-23 0.13947449321711503 1107 24-25 0.20153259228043652 1107 26-27 0.10501439222860398 1107 28-29 0.028831551715562398 1107 30-31 -0.021428762719331473 1107 32-33 0.10766381900467081 1107 34-35 0.02524207527405764 1107 36-37 0.04182249471075039 1107 38-39 0.06552316783645296 1107 40-41 0.07565380031439872 1107 42-43 0.039336989982061255 1107 44-45 0.14259502507322708 1107 46-47 0.2842693391346316 1107 48-49 0.11219041420380194 1107 50-51 0.1416619039058702 1107 52-53 0.15938989530209113 1107 54-55 0.2798117971630134 1107 56-57 0.1889056458778242 1107 58-59 0.04120479269833055 1107 60-61 0.1957699531111814 1107 62-63 0.04219965485585675 1107 64-65 -0.008876986760895988 1107 66-67 0.1556692800111037 1107 68-69 0.10042150073874012 1107 70-71 0.11952972472990808 1107 72-73 0.19489298850795223 1107 74-75 0.18982726436792063 1107 76-77 0.16713986966927763 1107 78-79 0.13593812493870416 1107 80-81 0.22628110011797276 1107 82-83 0.22195734428775182 1107 84-85 0.23429674889154484 1107 86-87 0.06695480460460601 1107 88-89 -0.18271257485923798 1107 90-91 -0.02654403472652689 1107 92-93 0.18356683610829094 1107 94-95 0.21598311128192904 1107 96-97 0.0013477673331792062 1107 98-99 0.06120419825781909 1107 100-101 0.2691637533885185 1108 1 0.10648281192442255 1108 2 0.08876915538063201 1108 3 0.05785731666181704 1108 4 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-0.8559838998027303 2316 70-71 -0.9033785460886676 2316 72-73 -1.0943841830263779 2316 74-75 -0.885048812695814 2316 76-77 -0.8362825350035408 2316 78-79 -0.984332644711408 2316 80-81 -1.2244155241286308 2316 82-83 -1.5611115589614997 2316 84-85 -1.485922105915506 2316 86-87 -1.0907774275021396 2316 88-89 -0.922858692100732 2316 90-91 -0.8399447911063547 2316 92-93 -0.7640153343645864 2316 94-95 -0.8976075263118375 2316 96-97 -0.7922164738452366 2316 98-99 -0.7147752821656823 2316 100-101 -0.970203313599761 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 20.0 14 71.0 15 223.0 16 514.0 17 990.0 18 1648.0 19 2297.0 20 3188.0 21 4333.0 22 5410.0 23 5963.0 24 6540.0 25 7663.0 26 9357.0 27 11666.0 28 14719.0 29 18735.0 30 23461.0 31 29905.0 32 38603.0 33 51147.0 34 71695.0 35 111382.0 36 202986.0 37 413072.0 38 618588.0 39 265730.0 40 234.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.04622683929633 20.240739496043513 13.643381044604332 23.069652620055827 2 14.767969626819227 20.35529121953633 41.93498826893181 22.941750884712633 3 18.638542935024326 25.914545547342012 30.123835107341467 25.3230764102922 4 11.861919107322175 16.598957486968587 39.475962199527494 32.063161206181746 5 12.349718067579413 38.954326791626244 35.998293875293534 12.697661265500814 6 30.850598992469823 38.342080086826954 18.028311462543638 12.779009458159582 7 27.257685763701726 32.64312360394367 22.73864263093179 17.360548001422814 8 24.769691392455034 37.96294126316766 20.21393220602029 17.053435138357028 9 25.40756121555657 16.66388041294884 20.762225274081434 37.16633309741316 10-11 24.060420562864916 27.138033092361447 29.227957738520242 19.573588606253395 12-13 24.49825299287917 24.155569825340955 29.606914283898945 21.739262897880938 14-15 21.520164404593203 26.322650263704595 26.629346490700424 25.52783884100178 16-17 21.58888331638145 29.631157295219467 27.160245002840938 21.619714385558144 18-19 21.5022750933395 28.811217509547475 28.67013412035887 21.016373276754155 20-21 22.230763261656307 27.91573639644172 28.627298724416594 21.226201617485383 22-23 22.0562011224392 27.682667188860606 28.364166732635315 21.896964956064878 24-25 21.85245771013691 27.859620725769616 28.288599639297324 21.99932192479615 26-27 21.722857852626447 28.061168424610482 28.34172073821662 21.874252984546448 28-29 21.679593185799632 27.886692535332397 28.594017701751888 21.839696577116076 30-31 21.98949825122781 27.873727943710087 28.24371912236613 21.893054682695972 32-33 21.692058471762873 28.00938264673861 28.379876368421996 21.91868251307652 34-35 21.877143397281763 28.030806071012492 28.241233326094285 21.850817205611463 36-37 22.02280978323988 27.89006119863073 28.076479800181342 22.010649217948057 38-39 21.982083607401748 27.866026505345182 28.427079053048708 21.724810834204362 40-41 21.766630679726124 27.869619991448545 28.433068196554316 21.930681132271015 42-43 21.86766492669028 27.77077308385165 28.25974759145292 22.101814398005146 44-45 21.970339678179883 27.833867408695507 28.074058103024726 22.121734810099884 46-47 21.968256497830108 27.82342546719225 28.03804512272797 22.170272912249672 48-49 21.944912594197955 27.75117934665107 28.149018361600316 22.154889697550658 50-51 21.863439541004936 27.956098479452546 28.126442921069923 22.054019058472598 52-53 21.933311147462607 27.811655498216016 28.13983452256891 22.115198831752462 54-55 21.965450076036912 27.865185509239005 28.010645168010335 22.158719246713748 56-57 22.09759595779685 27.83670574192208 27.877770434567044 22.187927865714027 58-59 21.99275990669435 27.75775320666555 28.059762277874384 22.189724608765708 60-61 21.98759517212161 27.90476941909689 28.014318751319887 22.093316657461607 62-63 21.83894307761774 27.965576486310116 28.072834234569232 22.12264620150291 64-65 21.93924821145947 27.867120175028816 27.985496898404854 22.20813471510686 66-67 21.918859083786035 27.829284411925997 27.94346873484812 22.30838776943985 68-69 21.96734510642708 27.71994348331711 27.93779206839498 22.37491934186083 70-71 21.904953854951277 27.568235874344644 28.00960971095352 22.51720055975056 72-73 21.873159314862814 27.791474688577555 27.779730759355694 22.555635237203933 74-75 21.708015192634292 27.759393711191 27.826185681155707 22.706405415019002 76-77 21.609246404300517 27.691820548595132 27.989038304999475 22.70989474210488 78-79 21.818618814425875 27.74232305782985 27.74190642002403 22.69715170772025 80-81 21.841347688874837 27.79090050622191 27.626856272875433 22.740895532027817 82-83 21.842892049187608 27.597365987573674 27.830825091085508 22.728916872153214 84-85 21.965027568287955 27.41712717972274 27.79019874061332 22.82764651137599 86-87 21.62757839137855 27.641954418972357 27.883316902248325 22.84715028740077 88-89 21.72429003911692 27.41861144572195 27.719422688229667 23.13767582693146 90-91 21.754574273451794 27.577818503953615 27.608727692393426 23.058879530201168 92-93 21.957840596081223 27.51316179384743 27.682289998494902 22.84670761157644 94-95 21.74626759180706 27.344424185515543 27.77730906219908 23.13199916047832 96-97 21.641379461195818 27.290938530035035 27.898784516345415 23.168897492423735 98-99 21.675621738195268 27.241358837710354 27.787178129106145 23.295841294988232 100-101 21.693511049448976 27.224667355157774 27.739837855657473 23.34198373973578 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13.0 1 14.0 2 32.0 3 60.5 4 51.0 5 36.0 6 67.0 7 166.5 8 296.5 9 382.0 10 352.0 11 286.0 12 298.0 13 328.5 14 355.5 15 403.0 16 478.5 17 511.5 18 561.0 19 656.0 20 773.0 21 910.5 22 1037.5 23 1229.0 24 1543.5 25 1997.0 26 2709.0 27 4154.5 28 7008.0 29 12508.0 30 20892.5 31 31359.0 32 43622.0 33 56013.5 34 65111.0 35 73273.0 36 83224.5 37 90811.0 38 88724.5 39 81681.5 40 75170.0 41 67330.5 42 61328.5 43 58076.0 44 56632.0 45 55746.0 46 54820.5 47 55229.0 48 58257.5 49 59868.0 50 58842.5 51 58156.5 52 57655.5 53 57617.5 54 56097.0 55 52901.0 56 49548.0 57 45185.5 58 41172.0 59 37215.5 60 31849.5 61 26026.0 62 20563.5 63 15328.5 64 10872.5 65 7481.0 66 4972.5 67 3442.0 68 2484.0 69 1767.0 70 1333.0 71 964.0 72 634.5 73 480.5 74 379.0 75 274.5 76 180.0 77 119.5 78 89.5 79 48.5 80 23.0 81 16.0 82 9.5 83 7.0 84 5.0 85 5.5 86 5.0 87 2.5 88 1.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046246603765036004 2 0.001354067227354658 3 3.645565612108694E-4 4 0.008593118942827636 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0019269418235431668 24-25 0.0 26-27 0.0 28-29 0.023123301882518002 30-31 0.019035060446081824 32-33 2.603975437220496E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03364336264888881 50-51 0.010806498064465058 52-53 0.0 54-55 0.0010936696836326082 56-57 0.0 58-59 0.0 60-61 7.811926311661488E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.166360699552794E-4 80-81 0.0120824460287031 82-83 0.0054423086637908355 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1920141.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.65675161843454 #Duplication Level Percentage of deduplicated Percentage of total 1 64.86005170172432 29.612992705044494 2 16.416711126801655 14.990674046159477 3 6.975832856231961 9.554816041460922 4 3.650055297880952 6.665986725156067 5 2.1952686714178062 5.011441823332679 6 1.415702320226519 3.8781821520134185 7 0.9342790285986163 2.9859301885727527 8 0.6826589633030857 2.4934392582101608 9 0.514682184580082 2.1148844997425478 >10 2.2402015692069353 16.866127733601438 >50 0.08105906338387595 2.54230168132309 >100 0.03177101269855072 2.6077443522076704 >500 0.0012658828935162493 0.3963236260828712 >1k 4.603210521877268E-4 0.27915516709244254 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.2079508744409915E-5 0.0 8 0.0 0.0 0.0 5.2079508744409915E-5 0.0 9 0.0 0.0 0.0 2.0831803497763966E-4 0.0 10-11 0.0 0.0 0.0 3.124770524664595E-4 0.0 12-13 0.0 0.0 0.0 6.770336136773289E-4 0.0 14-15 0.0 0.0 0.0 0.001328027472982453 0.0 16-17 0.0 0.0 0.0 0.0031508102790367997 0.0 18-19 0.0 0.0 0.0 0.005155871365696582 0.0 20-21 0.0 0.0 0.0 0.00960866936334363 0.0 22-23 0.0 0.0 0.0 0.018878821919848597 0.0 24-25 0.0 0.0 0.0 0.027992735950120327 0.0 26-27 0.0 0.0 0.0 0.032367414684650764 0.0 28-29 0.0 0.0 0.0 0.03637753685797032 0.0 30-31 0.0 0.0 0.0 0.04403322464339858 0.0 32-33 0.0 0.0 0.0 0.05942271947737172 0.0 34-35 0.0 0.0 0.0 0.0850979172883658 0.0 36-37 0.0 0.0 0.0 0.12322011768927386 0.0 38-39 0.0 0.0 0.0 0.179648265413842 0.0 40-41 0.0 0.0 0.0 0.24604963906296462 0.0 42-43 0.0 0.0 0.0 0.311904177870271 0.0 44-45 0.0 0.0 0.0 0.38989324221502486 0.0 46-47 0.0 0.0 0.0 0.48105842227211437 0.0 48-49 0.0 0.0 0.0 0.5819103909556642 0.0 50-51 0.0 0.0 0.0 0.6893243777410096 0.0 52-53 0.0 0.0 0.0 0.7959832116495611 0.0 54-55 0.0 0.0 0.0 0.9137870604294164 0.0 56-57 0.0 0.0 0.0 1.0532039053382016 0.0 58-59 0.0 0.0 0.0 1.2042605204513626 0.0 60-61 0.0 0.0 0.0 1.3716440615558962 0.0 62-63 0.0 0.0 0.0 1.5342883673646883 0.0 64-65 0.0 0.0 0.0 1.705629951133797 0.0 66-67 0.0 0.0 0.0 1.9020998978720836 0.0 68-69 0.0 0.0 0.0 2.129817549857016 0.0 70-71 0.0 0.0 0.0 2.382845843091731 0.0 72-73 0.0 0.0 0.0 2.642904870006942 0.0 74-75 0.0 0.0 0.0 2.8950478115930025 0.0 76-77 0.0 0.0 0.0 3.1702619755528367 0.0 78-79 0.0 0.0 0.0 3.5007064585361176 0.0 80-81 0.0 0.0 0.0 3.8628413225903726 0.0 82-83 0.0 0.0 0.0 4.243151935196426 0.0 84-85 0.0 0.0 0.0 4.608698007073439 0.0 86-87 0.0 0.0 0.0 4.989242977468842 2.6039754372204958E-5 88-89 0.0 0.0 0.0 5.4337676243567525 5.2079508744409915E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6315 0.0 26.41535 1 ACATGGG 6060 0.0 25.472593 3 TACATGG 6700 0.0 23.89132 2 GTATCAA 5305 0.0 23.74013 1 GGTATCA 3965 0.0 23.013393 1 TCAACGC 5535 0.0 22.482578 4 CAACGCA 5650 0.0 22.10788 5 ATCAACG 5745 0.0 21.660759 3 AACGCAG 5820 0.0 21.462116 6 GTCTACG 135 3.7837276E-4 21.122244 1 CATGGGG 3255 0.0 20.866396 4 CGTAGCG 70 4.8462834E-4 20.362309 42-43 TATCAAC 6300 0.0 19.75358 2 GCACCGT 315 3.2196112E-10 19.600746 6 CATGGGA 3025 0.0 18.998636 4 TGCACCG 300 3.7252903E-9 18.997644 5 ACGCAGA 6550 0.0 18.925137 7 CGCAGAG 6670 0.0 18.371037 8 TAGGTCG 285 4.2826287E-8 18.331062 5 ATGGGAG 1460 0.0 17.566317 5 >>END_MODULE