FastQCFastQC Report
Fri 10 Feb 2017
SRR2031658_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031658_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422693
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT100460.7061256363811448No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC72920.512549088243212No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC72590.5102295435487488No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC70960.4987723985427637No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT63520.4464772090675922No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT49710.3494077780659636No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC48050.33773976536048184No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA44410.3121544844882206No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT42650.29978357945108325No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42090.2958473823938123No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT39150.2751823478431397No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC36580.25711801491959263No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA34040.23926454969554217No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT33730.23708558346741004No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA33410.23483632800611237No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC33300.23406314644129128No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG33060.23237620484531799No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG31530.22162195217098837No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA30980.217756044346883No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT29560.20777497323737448No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29460.20707208090571896No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA29230.20545542854291124No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA28810.20250328074995802No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT28330.19912939755801143No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC28000.19680985286354824No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC27810.19547435743340272No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA27670.19449030816908497No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC27480.19315481273893945No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC26400.18556357555705974No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT26200.1841577908937487No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA26150.1838063447279209No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT26070.1832440308625965No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT26050.18310345239626538No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC25960.1824708492977754No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT25730.1808541969349677No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA25720.18078390770180214No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT24100.16939705192898258No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA23900.1679912672656715No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG23250.16342246710991057No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC22890.16089205471595067No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG22750.15990800545163292No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC22730.15976742698530183No Hit
ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC22620.15899424542048074No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22580.1587130884878185No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT22540.15843193155515634No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG22490.15808048538932853No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC22450.15779932845666633No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG22220.1561826760938586No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG22200.15604209762752752No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC22180.1559015191611964No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA22170.15583122992803086No Hit
CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT22080.1551986268295409No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA21850.15358197446673316No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG21740.15280879290191207No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG21250.1493646204768No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC21070.14809941427982004No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT21060.14802912504665447No Hit
ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA20160.14170309406175471No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC19870.13966470629995367No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA19520.13720458313915934No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC19470.13685313697333157No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA19380.1362205338748416No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT19160.1346741707451994No Hit
TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT19130.13446330304570275No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA18730.13165173371908065No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG18570.13052710598843179No Hit
ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC18460.12975392442361072No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT18420.12947276749094852No Hit
GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT18220.12806698282763745No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT18220.12806698282763745No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC18210.12799669359447188No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT18070.12701264433015413No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA17910.1258880165995053No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA17840.12539599196734644No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC17650.12406049653720093No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG17550.12335760420554541No Hit
TATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAA16810.11815620095129448No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC16790.11801562248496339No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC16760.11780475478546672No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA16740.1176641763191356No Hit
GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC16740.1176641763191356No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16450.11562578855733457No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA16420.11541492085783792No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA16420.11541492085783792No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT16300.11457145005985128No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC16000.1124627730648847No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT15820.11119756686790473No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG15730.11056496376941477No Hit
ATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTT15670.11014322837042145No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT15620.10979178220459368No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC15620.10979178220459368No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC15430.10845628677444819No Hit
ATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGC15310.10761281597646154No Hit
CTACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTC15280.10740194827696488No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT15100.10613674207998493No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA15080.10599616361365383No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT14880.10459037895034276No Hit
GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCT14750.10367661891919058No Hit
GTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCA14350.10086504959256846No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT14270.10030273572724405No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG1350.052.882552
GACATAT3700.047.6329461
ACCTGGG1500.047.540623
CTTCGTC405.3790165E-447.517179
TAACTTT18150.045.033226
AACTTTA18300.044.1416367
CGGAACA554.9389277E-543.2187424
GTCTTAA459.494337E-442.3403931
GTATGGT1700.042.029071
ATGTATT1155.2750693E-1141.4199561
TGGGTAT1850.038.530156
ACATATA4600.038.2823682
ATGCGCA250.001601656938.0700656-57
CATATAG4500.038.0324943
CGCCGTC250.001614885238.00637494-95
ACTTTAT21800.037.0546728
GGGCCTA905.683123E-736.95787
AAGACAT1700.036.3545885
ATAACTT23550.035.7311635
CATTCCG3900.035.3332829
Produced by FastQC (version 0.11.5)