FastQCFastQC Report
Fri 10 Feb 2017
SRR2031657_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031657_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994394
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23600.031.7003171
GTATCAA34450.030.015561
TCAACGC34700.027.8157884
CAACGCA35450.027.0931765
ATCAACG35850.026.9228343
AACGCAG37550.025.8227866
CGCAGAG41650.023.2808078
ACGCAGA41550.023.2212727
GTACATG47500.023.1736411
TATCAAC42800.023.1308922
GACCGTA1302.9303294E-421.936587
CTATACG1100.002861563321.6123584
ACATGGG45950.021.5224513
TACATGG49050.021.1538932
TCTATAC11650.020.4060043
CCGTACC1404.8317036E-420.3707059
GTACCGG1200.004677768319.8547611
CCTATAC9250.019.0183943
GCAGAGT51150.018.4922759
CATGGGG25450.017.9352844
Produced by FastQC (version 0.11.5)