FastQCFastQC Report
Fri 10 Feb 2017
SRR2031657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994394
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGCG753.0813145E-431.6630558
GGTATCA24050.030.631321
GTATCAA35900.028.3314231
TCAACGC35650.027.1819154
ATCAACG36450.026.715653
CAACGCA36800.026.3285225
AACGCAG37650.025.8602646
TAGATCG1151.2832078E-424.7797855
CGCAGAG40300.023.5705138
ACGCAGA41300.023.3447957
GTACATG44250.022.8778611
TATCAAC44150.022.7029862
TACATGG45900.020.698962
TCGCGTG600.004768668719.78941234-35
ACATGGG44200.019.7744773
ATACCGT2051.5919122E-518.5344726
GCAGAGT52750.018.0074259
TCTATAC10700.017.7576583
TACCGTG2253.54316E-516.8869657
GTGTAGC11050.016.774571
Produced by FastQC (version 0.11.5)