FastQCFastQC Report
Fri 10 Feb 2017
SRR2031655_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031655_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156737
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29950.030.0648781
GGTATCA21350.028.7863311
TCAACGC29700.028.4896814
ATCAACG30050.028.3153883
CAACGCA30750.027.6714525
AACGCAG33150.025.942826
GTACCGG1501.0334879E-625.4093281
GTACATG47350.023.6451641
TATCAAC36350.023.567172
ACGCAGA36450.022.9423167
CGCAGAG36750.022.8853868
TACATGG46300.022.716982
ACATGGG44000.022.0389793
TGGACCG3301.8189894E-1221.6073445
CCTATAC7250.020.9809883
CATGGGG28100.018.9467934
CTATACT13500.018.6623424
GCAGAGT46800.017.8693669
GGACCGT2152.3899125E-517.6796516
TATACTG18850.017.1483215
Produced by FastQC (version 0.11.5)