FastQCFastQC Report
Fri 10 Feb 2017
SRR2031655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156737
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19200.031.190971
GTATCAA28850.028.8304711
TCAACGC28400.028.4353434
ATCAACG29000.028.010833
CAACGCA31750.025.4303595
AACGCAG32550.024.9512566
TATCAAC35150.023.1104662
CGCAGAG35850.022.1245578
ACGCAGA36650.022.0303947
GTACATG47650.020.6474761
ACATGGG44500.020.0689983
TACATGG48100.019.653772
CCGTGCG1300.00756987718.2672829
GAGTACA36050.017.2713221
TATACCG1650.001465422617.2708845
GTATAGG8600.017.132581
GTATATA20450.016.9663751
GTATTAG9700.016.6596831
CATGGGG26500.016.3126074
AGAGTAC39250.016.27533510-11
Produced by FastQC (version 0.11.5)