FastQCFastQC Report
Fri 10 Feb 2017
SRR2031654_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031654_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993848
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC1151.2799757E-424.7889868
TATAACG1652.396433E-623.0686932
GTATCAA46350.022.9156061
GTACATG43300.022.6597731
TCAACGC45800.022.4153984
ATCAACG46750.022.1626743
CAACGCA46200.022.1184485
AACGCAG47200.021.8424456
ACATGGG41100.021.393273
CGTAGCA1404.799241E-420.3910772
TACATGG47200.020.2614732
GCACCGT3800.020.0041436
ACGCAGA51400.019.8717947
CGCAGAG52300.019.3501038
GTATTAC7400.019.3092651
TATCAAC55050.019.0143872
GTATTCG1600.001169866117.8610731
GTAATAC7350.017.4591733
GGTATCA35200.017.4551371
GTATAGG7800.017.0977761
Produced by FastQC (version 0.11.5)