FastQCFastQC Report
Fri 10 Feb 2017
SRR2031654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993848
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCT1252.2569325E-422.799674
ATACCGT2806.548362E-1122.0510396
GTACATG40450.021.6163671
GTATCAA40750.021.5738431
TCAACGC40800.020.9555784
CAACGCA41000.020.8512635
ATCAACG41450.020.6269633
GGTATCA30750.020.2446171
AACGCAG42900.020.038496
GTATAGG8100.019.946991
ACATGGG39750.019.59723
TACATGG44350.018.6360472
GATACCG1600.001192349417.8104555
ACGCAGA47700.017.7233317
TATCAAC49200.017.667872
TATTACG1350.00940218117.592782
CGCAGAG48650.017.4748698
GCACCGT2202.9218043E-517.2707446
AGAGTAC40000.017.09803610-11
TATATCG1952.2783656E-417.0493245
Produced by FastQC (version 0.11.5)