FastQCFastQC Report
Fri 10 Feb 2017
SRR2031653_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031653_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412742
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28300.035.8615721
TCAACGC40850.028.6377324
GTATCAA43850.028.2513941
ATCAACG41600.028.1208423
CAACGCA41900.028.0335755
AACGCAG43150.027.427646
ACGCAGA47650.024.4368937
GTCCTAC6850.024.3452151
GTACATG59700.024.0230581
CGCAGAG48750.023.790978
ACATGGG53050.023.5752813
TATCAAC51250.023.4035452
GGACCGC2354.622052E-922.248196
TACATGG64100.020.790982
AGGACCG3302.7284841E-1120.174825
GTGTACG2702.2215318E-819.4117661
GCAGAGT60800.019.1549
GGACCGT2251.6590493E-619.0120896
GCGTTAG1250.006013057719.0109067
GACCGTC2051.5827307E-518.5472247
Produced by FastQC (version 0.11.5)