FastQCFastQC Report
Fri 10 Feb 2017
SRR2031653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412742
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30100.033.1567041
GTATCAA44900.030.906871
TCAACGC43150.030.2735464
GTACGCG650.00584889529.2271239
CAACGCA44750.029.18635
ATCAACG45150.029.0377353
AACGCAG46500.028.3943066
GCCGCGC700.00839847627.1394718
CTGATCG2007.203198E-1026.1217429
ACGCAGA51000.025.9820527
CGCAGAG51050.025.9566048
TATCAAC54950.024.4641512
GTACATG58700.024.126631
ACATGGG53650.024.0830023
CGTAGAC1652.4308574E-623.0312483
TACATGG65200.020.9824562
GCAGAGT61850.020.8098559
CATGGGG26200.020.487484
GGGACGC1908.266334E-619.9975036
GACTGCG2803.4979166E-818.6583867
Produced by FastQC (version 0.11.5)