FastQCFastQC Report
Fri 10 Feb 2017
SRR2031652_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031652_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2738911
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT104480.38146548025839466No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT68670.2507200854646245No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT37010.13512669816580386No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT35200.1285182322463198No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC32850.11993817980942059No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT32750.11957307119508448No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC31760.11595849591315673No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAG700.00829235527.210441
CCTCGTC4150.022.8952528
ACATGGG51200.022.1816963
CGTCCGA2050.022.01513510-11
GCGTAGG1302.89387E-421.9776611
CTTCGGC2606.2573235E-1021.9266079
CCGTCCA2257.1486284E-821.114519
GTACATG61650.021.0091951
ATAGTCG2801.5752448E-920.3604188
GTCCGAC2300.019.62128812-13
CAGTGCG2702.2979293E-819.3549679
AGCCTCG5750.019.002716
TACATGG65300.018.650332
CGCCGGA3750.018.37264694-95
TCGTCCG2350.018.19391810-11
GTATAGG13400.018.1233711
TAGACAG19750.017.8048935
GCCGTCC1350.00939490917.5954258
CTCGTCC5700.017.5028159
GCGCCGG2450.017.45466294-95
Produced by FastQC (version 0.11.5)