FastQCFastQC Report
Fri 10 Feb 2017
SRR2031652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2738911
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT260810.9522397770500757No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT122060.4456515746586873No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT109440.399574867529467TruSeq Adapter, Index 3 (95% over 21bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT59800.2183349513730092No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG50960.1860593498656948TruSeq Adapter, Index 3 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC46830.1709803640936124No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37570.1371713064060862No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC37250.13600295884021057No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31490.11497270265444916No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT31030.11329320302850293RNA PCR Primer, Index 3 (95% over 23bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28250.10314318354995836No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT27770.1013906622011449No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCG458.949627E-426.39002278-79
CGTGCGA550.002878019121.59183580-81
CTGTACG3302.7284841E-1120.1494359
ACATGGG48750.019.9741213
GTACATG60750.018.774121
TAGACAG20600.018.4446985
GTCCGAC2153.6379788E-1217.67259412-13
TCGTCCG1751.3169483E-917.64103710-11
GTCGGCC2700.017.59334880-81
GAGTACG1901.8602697E-417.5081021
GTATAGG15600.017.3638061
TGCTTGA97850.017.3283154-55
CGTCCGA1701.7558705E-816.76297610-11
GCGTCCC850.001789958916.76297640-41
GGTCGGC2600.016.44301280-81
CAGTGCG3201.6071135E-716.326449
TACATGG63400.016.1081452
CTTGAAA105350.016.09468556-57
TAATACT22950.015.522694
CCGTGCA3102.0674597E-615.3209999
Produced by FastQC (version 0.11.5)