##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031651_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	795750
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.15792397109645	33.0	30.0	34.0	16.0	34.0
2	29.39452340559221	33.0	31.0	34.0	16.0	34.0
3	29.500011310084826	33.0	31.0	34.0	16.0	34.0
4	32.2847150486962	37.0	33.0	37.0	19.0	37.0
5	32.123021049324535	37.0	33.0	37.0	19.0	37.0
6	32.359475966069745	37.0	33.0	37.0	17.0	37.0
7	32.48019604147031	37.0	35.0	37.0	17.0	37.0
8	32.538993402450515	37.0	35.0	37.0	17.0	37.0
9	34.10417844800503	39.0	35.0	39.0	17.0	39.0
10-11	34.056333647502356	39.0	35.0	39.0	17.0	39.0
12-13	34.07693433867421	39.0	35.0	39.0	17.0	39.0
14-15	34.96483820295319	40.0	35.5	41.0	17.0	41.0
16-17	34.94719384228715	40.0	35.5	41.0	16.5	41.0
18-19	35.01898146402765	40.0	36.0	41.0	16.0	41.0
20-21	35.02466918001885	40.0	36.0	41.0	16.0	41.0
22-23	34.974276468740186	40.0	35.5	41.0	14.0	41.0
24-25	34.77730631479736	40.0	35.0	41.0	12.0	41.0
26-27	34.52226076028903	40.0	34.5	41.0	11.0	41.0
28-29	34.401497329563306	40.0	34.0	41.0	11.0	41.0
30-31	34.27487338988376	40.0	34.0	41.0	10.0	41.0
32-33	33.999511781338356	40.0	34.0	41.0	10.0	41.0
34-35	33.78674395224631	39.0	33.5	41.0	9.5	41.0
36-37	33.56970216776626	39.5	33.5	41.0	9.0	41.0
38-39	33.342985234055924	39.0	33.0	41.0	9.0	41.0
40-41	33.26436632108074	39.0	32.5	41.0	9.0	41.0
42-43	33.023157398680496	39.0	31.0	41.0	9.0	41.0
44-45	32.69490040841973	38.5	30.5	40.5	9.0	41.0
46-47	32.48459629280553	38.5	30.0	40.5	9.0	41.0
48-49	32.48980018850141	39.0	30.0	41.0	8.5	41.0
50-51	32.197276154571156	38.5	29.5	40.5	8.0	41.0
52-53	32.497313854853914	38.5	30.0	40.5	8.0	41.0
54-55	32.80476217404964	40.0	30.0	41.0	8.0	41.0
56-57	32.643653785736724	39.5	29.5	41.0	8.0	41.0
58-59	32.41997109644989	39.0	28.0	41.0	8.0	41.0
60-61	31.877896952560477	39.0	27.0	41.0	8.0	41.0
62-63	31.592930568645933	38.0	26.0	41.0	8.0	41.0
64-65	31.138571787621743	37.5	25.5	40.5	8.0	41.0
66-67	30.51218535972353	37.0	23.5	39.0	7.0	41.0
68-69	29.84621866163996	36.0	22.5	39.0	7.0	41.0
70-71	28.997482249450204	35.0	21.0	38.0	7.0	39.0
72-73	28.24955702167766	34.5	20.0	37.0	7.0	39.0
74-75	27.651874960728872	34.0	18.5	37.0	7.0	38.0
76-77	27.066561734213007	34.0	17.5	36.0	7.0	37.0
78-79	26.626529688972667	33.5	15.5	35.0	7.0	36.5
80-81	26.193857995601633	33.0	13.0	35.0	7.0	36.0
82-83	25.646381401193842	31.5	12.5	35.0	7.0	36.0
84-85	25.294417844800503	31.0	11.5	35.0	7.0	35.0
86-87	25.09088030160226	31.5	11.0	35.0	7.0	35.0
88-89	24.62380647188187	31.0	10.0	35.0	7.0	35.0
90-91	24.17668865849827	30.0	10.0	35.0	7.0	35.0
92-93	23.72536537857367	29.0	10.0	35.0	7.0	35.0
94-95	23.259459000942506	29.0	10.0	35.0	7.0	35.0
96-97	22.929868049010366	27.0	9.0	35.0	7.0	35.0
98-99	22.73590574929312	27.0	8.0	35.0	7.0	35.0
100-101	22.166325479107762	26.0	8.0	34.5	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.2620098137277722
1101	2	-0.48192022285756053
1101	3	0.059349253259682655
1101	4	-0.016629789199939182
1101	5	-0.3022744719908985
1101	6	-0.16721581433968424
1101	7	-0.20821388789315165
1101	8	-0.3362884724059043
1101	9	-0.19855159364833241
1101	10-11	-0.20672787123160674
1101	12-13	0.060598092505514956
1101	14-15	-0.10310745071432592
1101	16-17	0.010476301944088107
1101	18-19	-0.21736035180464341
1101	20-21	-0.3886769041425566
1101	22-23	-0.1772765625947983
1101	24-25	-0.16582540974532378
1101	26-27	-0.22987734175305974
1101	28-29	-0.2685839541497401
1101	30-31	-0.16870784634694047
1101	32-33	-0.089338791153736
1101	34-35	-0.06859300564165949
1101	36-37	-0.22474724361714493
1101	38-39	0.06455374921036139
1101	40-41	-0.16501849881579034
1101	42-43	-0.02668903383824528
1101	44-45	-0.09796517736932486
1101	46-47	0.01641251175743008
1101	48-49	0.04783252719459341
1101	50-51	-0.05001791769824848
1101	52-53	-0.03415021857743028
1101	54-55	-0.06895473904862115
1101	56-57	0.1502607212828977
1101	58-59	0.27625948899440544
1101	60-61	0.12022653126086169
1101	62-63	-0.0037608781467284302
1101	64-65	0.020759889676476462
1101	66-67	0.33257021301462686
1101	68-69	-0.039258631678993794
1101	70-71	0.09330424904942092
1101	72-73	0.3263238670182709
1101	74-75	0.11348751397355272
1101	76-77	-0.03127825049234545
1101	78-79	-0.0150047773596107
1101	80-81	-0.15453415886102562
1101	82-83	0.233535559729372
1101	84-85	0.2581788975366841
1101	86-87	0.16686199232730559
1101	88-89	0.34858323157008186
1101	90-91	0.152644101186862
1101	92-93	-0.2905122344601523
1101	94-95	-0.3789825567295395
1101	96-97	-0.2156065006691179
1101	98-99	-0.14142468769127348
1101	100-101	-0.31724736616982696
1102	1	-0.7702905829981148
1102	2	-0.6894539478917743
1102	3	-0.7688177100981051
1102	4	-1.0286663593819654
1102	5	-0.8390504513288484
1102	6	-0.7191487655760085
1102	7	-0.8618775945085098
1102	8	-0.6514927776856361
1102	9	-0.7862186753395761
1102	10-11	-0.754733952542118
1102	12-13	-0.712047329018823
1102	14-15	-0.803530878680121
1102	16-17	-0.7780618168626958
1102	18-19	-0.8181084250273258
1102	20-21	-0.9611947313345581
1102	22-23	-0.9970662891930715
1102	24-25	-0.7417453237220499
1102	26-27	-0.7688013305165313
1102	28-29	-0.8670168076323392
1102	30-31	-0.8266920102439173
1102	32-33	-0.7039511719199254
1102	34-35	-0.6253055994080583
1102	36-37	-1.0084336908352824
1102	38-39	-0.8865758207761587
1102	40-41	-1.0628144096232433
1102	42-43	-0.8813935603500838
1102	44-45	-1.0950055548015953
1102	46-47	-1.2184832519049422
1102	48-49	-0.9106645864981289
1102	50-51	-0.8078600814511638
1102	52-53	-0.621289944661207
1102	54-55	-0.7230326127508135
1102	56-57	-0.6949025274334417
1102	58-59	-0.5133629243906519
1102	60-61	-0.69510639733776
1102	62-63	-1.1538848710261433
1102	64-65	-0.9439150020226883
1102	66-67	-1.1854704936933338
1102	68-69	-1.0150182810003763
1102	70-71	-1.0898424947226353
1102	72-73	-1.0539188194598168
1102	74-75	-0.9493771331355134
1102	76-77	-0.8025345401726369
1102	78-79	-0.6495293341236561
1102	80-81	-0.94773615921088
1102	82-83	-1.3224786094154375
1102	84-85	-0.8635347766655954
1102	86-87	-0.7257105254998848
1102	88-89	-1.020044769211946
1102	90-91	-0.6312365672428051
1102	92-93	-0.7331500929333394
1102	94-95	-0.7968089769882241
1102	96-97	-0.9737959473830529
1102	98-99	-0.8007555377370643
1102	100-101	-0.6700251153646484
1103	1	0.1770009036702902
1103	2	0.19807141930467154
1103	3	0.26521451879940017
1103	4	0.5413119403628599
1103	5	-0.23259864397888919
1103	6	0.12822610633220677
1103	7	0.3672308866745553
1103	8	0.18698097103344935
1103	9	0.3007782504576042
1103	10-11	0.3331043604639632
1103	12-13	0.19145686033599674
1103	14-15	0.4645426208878831
1103	16-17	0.25889391863530165
1103	18-19	0.23908078914213604
1103	20-21	0.09071006884225596
1103	22-23	0.29084134407261786
1103	24-25	0.4280817774855308
1103	26-27	0.14408620992028176
1103	28-29	0.2729096526139969
1103	30-31	0.3793624614151412
1103	32-33	0.26692678423752625
1103	34-35	0.38325896889751476
1103	36-37	0.300870293572693
1103	38-39	0.3802494117983315
1103	40-41	0.5294719533738075
1103	42-43	0.2636515480507242
1103	44-45	0.4526621700873861
1103	46-47	0.6504436905692899
1103	48-49	0.9157710461245827
1103	50-51	0.9549114228989382
1103	52-53	0.5714655858899391
1103	54-55	0.6498651750287223
1103	56-57	0.8630301214041864
1103	58-59	0.8998586593261209
1103	60-61	1.0830838415659798
1103	62-63	0.9855191756695909
1103	64-65	0.6726635910774341
1103	66-67	0.39242940383324054
1103	68-69	0.6521791712289229
1103	70-71	0.6826413807821936
1103	72-73	0.8139884119492926
1103	74-75	0.3372546054969696
1103	76-77	-0.010113555222165616
1103	78-79	0.10702092808775987
1103	80-81	0.438970425209142
1103	82-83	0.23464781466381268
1103	84-85	0.428956533185918
1103	86-87	0.4651386979122769
1103	88-89	0.4200911587753531
1103	90-91	0.7044919987319993
1103	92-93	0.6075514481866335
1103	94-95	0.18859107123456553
1103	96-97	0.24852869245299303
1103	98-99	-0.02144512362385953
1103	100-101	0.21313006155271097
1104	1	0.3671416824263538
1104	2	0.16851258885126086
1104	3	0.25810024788269104
1104	4	0.23336900519641546
1104	5	0.05413331138623789
1104	6	-0.13608953941382396
1104	7	-0.029364998254109764
1104	8	-0.0766472600508763
1104	9	0.2616803644931096
1104	10-11	0.41739505211611316
1104	12-13	0.13608937599115478
1104	14-15	0.17887239159271928
1104	16-17	0.09060915946584913
1104	18-19	0.15473595181457966
1104	20-21	-0.02335368180513342
1104	22-23	0.1301487502593517
1104	24-25	0.055557176515613094
1104	26-27	0.051191064197794844
1104	28-29	0.09043637232772994
1104	30-31	0.1747553706858227
1104	32-33	-0.08416554590984049
1104	34-35	0.06649970527869442
1104	36-37	-0.09770073142849611
1104	38-39	0.054723257082955
1104	40-41	-0.26569966487564045
1104	42-43	-0.12660223037575236
1104	44-45	-0.180006505462309
1104	46-47	0.2983790924243692
1104	48-49	0.2650389024266744
1104	50-51	0.22897564437828777
1104	52-53	-0.05793316169705065
1104	54-55	0.13729631585456303
1104	56-57	0.02764239128507029
1104	58-59	-0.03294891165180047
1104	60-61	-0.02778060591271725
1104	62-63	-0.15138463336096564
1104	64-65	-0.12544366444307187
1104	66-67	0.035006737982683944
1104	68-69	-0.1314029183233174
1104	70-71	-0.1901657193637618
1104	72-73	-0.15127360603648654
1104	74-75	-0.30673287037289043
1104	76-77	0.09636744670824626
1104	78-79	-0.05688744801280876
1104	80-81	-0.17504388808244897
1104	82-83	-0.2963188033502213
1104	84-85	0.020161392171747394
1104	86-87	0.22427283793770414
1104	88-89	0.17073619856065392
1104	90-91	-0.146962872024627
1104	92-93	-0.08384645190282569
1104	94-95	-0.374114329208016
1104	96-97	0.059303681859269375
1104	98-99	-0.11705461559617447
1104	100-101	-0.11563306288817543
1105	1	-0.1630118975001018
1105	2	0.041668548185025855
1105	3	-0.006078856594921689
1105	4	-0.07342312112847793
1105	5	-0.11962831526312101
1105	6	-0.16103005423104833
1105	7	-0.15274808283122354
1105	8	0.05592898913796063
1105	9	-0.05151453059666267
1105	10-11	-0.10213771881185352
1105	12-13	0.012576505560694784
1105	14-15	-0.06477891708129846
1105	16-17	-0.08394199779134226
1105	18-19	-0.11352059155552041
1105	20-21	-0.1468773677233628
1105	22-23	-0.014681446582528679
1105	24-25	-0.024942499019751097
1105	26-27	-0.17658310841738967
1105	28-29	-0.1720338850808787
1105	30-31	0.007266726947989355
1105	32-33	-0.19277467418152838
1105	34-35	-0.050750997728009395
1105	36-37	0.24860037175125882
1105	38-39	0.12027592850843405
1105	40-41	0.007618526774798795
1105	42-43	-0.029598120490398117
1105	44-45	0.2498550562941304
1105	46-47	0.22349265504604432
1105	48-49	0.112410738896493
1105	50-51	0.01992308110768448
1105	52-53	-0.12229612946689628
1105	54-55	0.24794957131638284
1105	56-57	0.016502438873217784
1105	58-59	-0.15541267976774265
1105	60-61	0.00757522363519314
1105	62-63	0.09096881677963609
1105	64-65	-0.23413931661698584
1105	66-67	-0.1859320463564913
1105	68-69	0.04420815673616829
1105	70-71	-0.06332167869753036
1105	72-73	-0.100127164331969
1105	74-75	-0.2583335625641112
1105	76-77	-0.6468620579424176
1105	78-79	-0.59579075755207
1105	80-81	-0.49950852143741287
1105	82-83	-0.5375690737374121
1105	84-85	-0.41419941250054393
1105	86-87	-0.8636570777014931
1105	88-89	-0.5978556249305846
1105	90-91	-0.9762079509776918
1105	92-93	-0.8040930450241746
1105	94-95	-0.8554792437656644
1105	96-97	-0.7829416134035441
1105	98-99	-0.7057956928187572
1105	100-101	-0.8390918558797402
1106	1	0.24068498287327245
1106	2	0.1036926208944351
1106	3	0.3159550417101613
1106	4	0.2046903645015874
1106	5	0.1621820507403129
1106	6	-0.10218701812198105
1106	7	-0.14709836531709897
1106	8	-0.04005479862917127
1106	9	0.22284066468705532
1106	10-11	0.06710202572265445
1106	12-13	0.17983884896526803
1106	14-15	0.056094332734460295
1106	16-17	0.30630609849949764
1106	18-19	0.34423548803179926
1106	20-21	0.39911870203836486
1106	22-23	0.3128854902880178
1106	24-25	0.262640404443772
1106	26-27	0.37589171349369366
1106	28-29	0.22857407365161464
1106	30-31	0.08622767020517585
1106	32-33	0.30074043267181594
1106	34-35	0.2344860447676922
1106	36-37	0.26296416443119597
1106	38-39	0.24863505403041586
1106	40-41	0.1937214498895159
1106	42-43	0.22752543637523814
1106	44-45	0.3182964711799059
1106	46-47	0.5818873983523574
1106	48-49	0.4619378821045572
1106	50-51	-0.09393100879651683
1106	52-53	0.08835235984777512
1106	54-55	0.19325170838344263
1106	56-57	0.29176036075972434
1106	58-59	0.3114013708340764
1106	60-61	0.3719390163151246
1106	62-63	0.4677190010095593
1106	64-65	0.5567597253874368
1106	66-67	0.5692570963602819
1106	68-69	0.3449757813984142
1106	70-71	-0.0381988587515707
1106	72-73	0.3060752429389737
1106	74-75	0.23211104564026286
1106	76-77	0.07401733287065326
1106	78-79	0.11747379661152735
1106	80-81	0.22216919902439258
1106	82-83	0.16797624682264356
1106	84-85	0.19488312250314266
1106	86-87	-0.08685162449589257
1106	88-89	0.28779286438408036
1106	90-91	0.25439263855805194
1106	92-93	0.1941997577269916
1106	94-95	0.29393736149103233
1106	96-97	-0.27104036064009307
1106	98-99	-0.29966697751538973
1106	100-101	-0.1331535114926119
1107	1	0.4436263917468821
1107	2	0.4427428362158352
1107	3	0.4673477168316502
1107	4	0.5983413462881586
1107	5	0.4268397094657743
1107	6	0.45765850358795035
1107	7	0.480352150269006
1107	8	0.5647158544465611
1107	9	0.6253376027772148
1107	10-11	0.5672906788774128
1107	12-13	0.4493708706159296
1107	14-15	0.645979165415909
1107	16-17	0.8603774508759301
1107	18-19	1.0203397510004706
1107	20-21	0.7225966365332468
1107	22-23	0.9476677983754058
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2316	84-85	-0.6384488448443335
2316	86-87	0.014191715503450553
2316	88-89	0.31534343318059044
2316	90-91	0.24640298726692222
2316	92-93	0.10613738134871298
2316	94-95	0.2853821920541435
2316	96-97	0.19951991211996045
2316	98-99	0.735933345950059
2316	100-101	0.4164795847055771
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	1.0
6	49.0
7	1810.0
8	11963.0
9	14777.0
10	12562.0
11	13255.0
12	15094.0
13	16757.0
14	15213.0
15	13361.0
16	11118.0
17	10481.0
18	10316.0
19	10339.0
20	9909.0
21	8786.0
22	7059.0
23	6706.0
24	6828.0
25	7005.0
26	7953.0
27	9577.0
28	11338.0
29	13678.0
30	16782.0
31	20181.0
32	26063.0
33	33969.0
34	44813.0
35	58710.0
36	83551.0
37	128209.0
38	123214.0
39	14270.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.20138527788856	21.127380946370966	16.053371566464563	14.61786220927591
2	14.929714558775878	28.641585227007667	34.31939308278791	22.109307131428544
3	25.331161261245384	30.03250554558204	31.164534460662246	13.47179873251033
4	11.983151585362782	19.123202458004886	41.03092653688267	27.862719419749666
5	9.247371145511773	44.221306158742635	31.98039741705364	14.55092527869195
6	21.664039761111056	39.31835577533159	16.94895410552607	22.06865035803128
7	20.38494945807475	36.91637608731227	21.985926308236508	20.712748146376462
8	22.930154394224537	27.769560483134963	21.6635301078179	27.6367550148226
9	23.126714359265502	22.770215749635256	22.54450882623588	31.558561064863362
10-11	24.929591650457326	24.947657986012754	25.754411144474034	24.368339219055894
12-13	31.970573758832217	25.173434307704124	24.232401342912134	18.62359059055153
14-15	25.13182667679693	26.842536284203018	30.84149388249942	17.184143156500628
16-17	17.90581223443624	30.67783724435607	30.6114954324763	20.804855088731387
18-19	17.661356472329686	34.99919859959208	27.9422237447084	19.397221183369844
20-21	22.983532475105186	27.52253225895635	24.89613545996974	24.597799805968723
22-23	20.370782280867104	28.50135092679862	25.344894753377318	25.78297203895696
24-25	24.639396795475964	29.547093936537856	24.205152371976123	21.608356896010054
26-27	22.938799874332393	23.855922086082312	32.045680175934656	21.159597863650646
28-29	19.322463085139805	31.919384228715046	28.454037071944704	20.30411561420044
30-31	21.26302230600063	27.32968897266729	27.09311969839774	24.31416902293434
32-33	19.82023248507697	28.423562676720078	29.400754005655045	22.35545083254791
34-35	22.506063462142635	26.466603832862077	28.48105560791706	22.546277097078228
36-37	21.7229657555765	24.7229657555765	32.407414388941255	21.14665409990575
38-39	20.3090794847628	27.32101790763431	30.78894125039271	21.580961357210178
40-41	18.74388941250393	28.37869934024505	30.297643732327995	22.57976751492303
42-43	18.418535972353126	29.230662896638393	27.1813383600377	25.169462770970778
44-45	19.41676775404811	26.134779260392705	28.507144906454325	25.941308079104864
46-47	21.291873861190897	28.858162467370402	28.786260878106027	21.06370279333267
48-49	19.538915155886368	28.51820387815327	29.314786646234374	22.62809431972599
50-51	15.006522552314014	29.196515856874772	32.80432028718091	22.99264130363031
52-53	18.32039423386448	30.41876659826171	26.249647915660713	25.0111912522131
54-55	17.051222076347706	26.315825876127384	28.00790740489233	28.62504464263258
56-57	21.38029889347399	24.112052661404803	27.813785629690845	26.693862815430354
58-59	25.259449639131855	24.162538761018318	26.188833682452383	24.389177917397447
60-61	20.87218868268804	25.727301041001425	29.616348976144106	23.784161300166424
62-63	20.532723364619002	25.411234500179802	28.10023169826198	25.955810436939213
64-65	20.152707007271072	29.609002868289576	25.07095226081491	25.16733786362444
66-67	22.066972526107588	25.457861520513443	23.140646078280312	29.334519875098657
68-69	21.683690957702066	25.99839117776731	24.49221155194741	27.825706312583215
70-71	25.038688179872842	26.66771050865917	23.44154704929135	24.852054262176637
72-73	23.501148380290235	23.17967734694917	28.42852660891128	24.890647663849315
74-75	21.27043564588154	29.755042774403556	24.874331708925183	24.100189870789716
76-77	22.850199727401215	25.262793413560058	25.887881945209536	25.999124913829196
78-79	23.636990442183485	25.308491691877588	27.06246709054982	23.99205077538911
80-81	26.156414674329987	25.177832555682716	25.796159118634687	22.86959365135261
82-83	24.333663258004027	24.183625088193015	29.104135041898754	22.3785766119042
84-85	23.31083396902999	26.241935352008245	27.31487391824134	23.132356760720423
86-87	24.299030163944735	26.139675254247948	26.15118125088417	23.410113330923153
88-89	24.753123414081664	24.715643073966444	24.61798037564608	25.91325313630581
90-91	26.464122696284054	22.323928203250944	25.34510531317832	25.86684378728669
92-93	28.533501975886633	23.78904578519469	25.52165924136022	22.155792997558457
94-95	26.41550739097231	23.089098048491422	25.882934648178647	24.612459912357625
96-97	23.945550338769664	23.770159844947006	27.874511214552445	24.40977860173089
98-99	25.445305421008317	25.397956251379444	23.86731417022938	25.289424157382857
100-101	24.454077094027305	24.146216495720317	24.401017191031368	26.99868921922101
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	158.0
1	100.5
2	63.5
3	123.0
4	107.0
5	62.0
6	94.5
7	160.5
8	207.5
9	194.5
10	143.0
11	105.0
12	111.5
13	115.0
14	120.0
15	136.0
16	159.0
17	192.5
18	224.5
19	266.5
20	333.0
21	443.5
22	585.5
23	756.5
24	1002.0
25	1282.0
26	1651.5
27	2071.0
28	2546.0
29	3190.0
30	3891.5
31	4816.5
32	6167.5
33	8075.0
34	10752.0
35	12770.0
36	14802.5
37	17060.5
38	19628.0
39	22263.5
40	21636.0
41	20382.5
42	19806.5
43	17896.5
44	17197.0
45	19979.0
46	30140.0
47	56827.0
48	100491.0
49	103073.5
50	72459.5
51	53552.0
52	38829.5
53	28794.0
54	17719.5
55	9695.0
56	5573.5
57	3729.5
58	2958.0
59	2550.5
60	2258.0
61	1998.0
62	1721.5
63	1506.5
64	1320.0
65	1139.5
66	975.5
67	845.0
68	718.0
69	609.5
70	539.0
71	440.5
72	360.5
73	286.5
74	211.5
75	163.5
76	134.0
77	107.5
78	65.5
79	46.5
80	46.0
81	36.5
82	23.5
83	11.5
84	6.0
85	6.0
86	4.5
87	4.0
88	3.5
89	4.5
90	4.5
91	3.0
92	1.5
93	1.5
94	1.0
95	1.0
96	1.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4295318881558278
2	0.3301288092994031
3	0.1789506754633993
4	0.2023248507697141
5	0.222808671065033
6	0.1766886584982721
7	0.17090794847628024
8	0.17027961043041157
9	0.17053094564875904
10-11	0.1828463713477851
12-13	0.17053094564875904
14-15	0.1797675149230286
16-17	0.07860508953817154
18-19	0.03361608545397424
20-21	2.513352183474709E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.13295633050581213
46-47	0.2021991831605404
48-49	0.07584040213634935
50-51	0.05435124096764059
52-53	0.061074458058435435
54-55	0.06861451460885956
56-57	0.09858623939679548
58-59	0.08212378259503614
60-61	0.13603518693056865
62-63	0.22990889098334905
64-65	0.12887213320766572
66-67	0.09079484762802388
68-69	0.09531888155827835
70-71	0.0767200754005655
72-73	0.06277097078228086
74-75	0.12554194156456172
76-77	0.19384228715048696
78-79	0.12007540056550425
80-81	0.077913917687716
82-83	0.07778825007854226
84-85	0.08677348413446434
86-87	0.06440464970153942
88-89	0.0837574615142947
90-91	0.10197926484448634
92-93	0.1470311027332705
94-95	0.11674520892240026
96-97	0.08382029531888155
98-99	0.074583726044612
100-101	0.05309456487590324
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	795750.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.522411602641476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.07815007597402	30.40670150122003
2	5.152945216174127	3.557841914372511
3	2.0443229735259965	2.1172487742240116
4	1.0822846835659214	1.4945230926918927
5	0.6470609949580994	1.1169052999979114
6	0.443207559299647	0.9180356272526727
7	0.3022588919298725	0.730429411443297
8	0.2253116804909144	0.6222642058232153
9	0.1890985989736168	0.5875325700525031
>10	1.4117940897250487	10.00986850578099
>50	0.20451830628879036	5.010872561448529
>100	0.189244625815896	13.781539267359832
>500	0.01378356526611841	3.3173897724129757
>1k	0.011920921311237544	9.809288137275725
>5k	0.002980230327809386	7.983727066548754
>10k+	0.0011175863729285195	8.535832292095137
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	34473	4.332139491046183	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	20150	2.5322023248507697	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	11749	1.476468740182218	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	9916	1.2461200125667609	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	9475	1.1907005969211435	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	9186	1.154382657869934	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	8272	1.0395224630851398	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	7134	0.8965127238454289	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	6713	0.8436066603832862	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	5857	0.7360351869305687	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	5526	0.6944392082940622	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	4968	0.6243166823751178	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	4889	0.6143889412503928	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	4882	0.6135092679861766	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	4633	0.5822180333019165	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	4622	0.5808356896010053	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	4589	0.5766886584982721	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	4108	0.5162425384857053	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	3953	0.4967640590637763	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	3591	0.45127238454288404	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	3004	0.37750549795790134	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2606	0.3274897895067546	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	2571	0.32309142318567385	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2158	0.2711907005969212	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	2127	0.26729500471253537	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	2040	0.2563619227144204	No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT	1911	0.2401508011310085	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	1861	0.2338674206723217	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	1587	0.1994344957587182	No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG	1543	0.19390512095507384	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	1328	0.1668865849827207	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	1229	0.15444549167452087	No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT	1189	0.14941878730757147	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	1183	0.14866478165252905	No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT	1154	0.14502042098649073	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	1148	0.14426641533144832	No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC	1114	0.1399937166195413	No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA	1108	0.1392397109644989	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	1073	0.13484134464341815	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	1060	0.1332076657241596	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1019	0.12805529374803645	No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA	1015	0.1275526233113415	No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG	1011	0.12704995287464657	No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT	1000	0.12566760917373546	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	996	0.12516493873704054	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	986	0.12390826264530316	No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT	923	0.11599120326735785	No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	921	0.11573986804901037	No Hit
GTGTAGGGTTGCAAGTCAGCTAAATACTTTGACACCGGTAGGAATTGCGA	885	0.1112158341187559	No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC	849	0.10669180018850141	No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA	818	0.10279610430411561	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2566760917373548E-4	0.0
2	0.0	0.0	0.0	0.0011310084825636192	0.0
3	0.0	0.0	0.0	0.00138234370091109	0.0
4	0.0	0.0	0.0	0.002387684574300974	0.0
5	0.0	0.0	0.0	0.00276468740182218	0.0
6	0.0	0.0	0.0	0.002890355010995916	0.0
7	0.0	0.0	0.0	0.0032673578385171223	0.0
8	0.0	0.0	0.0	0.0041470311027332705	0.0
9	0.0	0.0	0.0	0.008419729814640276	0.0
10-11	0.0	0.0	0.0	0.012755262331134151	0.0
12-13	0.0	0.0	0.0	0.019227144203581528	0.0
14-15	0.0	0.0	0.0	0.027332704995287463	0.0
16-17	6.283380458686774E-5	0.0	0.0	0.043857995601633684	0.0
18-19	1.2566760917373548E-4	0.0	0.0	0.05981778196669808	0.0
20-21	1.2566760917373548E-4	0.0	0.0	0.0921143575243481	0.0
22-23	1.2566760917373548E-4	0.0	0.0	0.2010053408733899	0.0
24-25	1.2566760917373548E-4	0.0	0.0	0.46748350612629597	0.0
26-27	1.2566760917373548E-4	0.0	0.0	0.8745208922400252	0.0
28-29	1.2566760917373548E-4	0.0	0.0	1.485579641847314	0.0
30-31	1.2566760917373548E-4	0.0	0.0	2.460823122840088	0.0
32-33	1.2566760917373548E-4	0.0	0.0	3.8311655670750864	0.0
34-35	1.2566760917373548E-4	0.0	0.0	5.941376060320453	0.0
36-37	1.2566760917373548E-4	0.0	0.0	8.050078542255735	0.0
38-39	1.2566760917373548E-4	0.0	0.0	10.530882814954445	0.0
40-41	1.2566760917373548E-4	0.0	0.0	13.506189129751807	0.0
42-43	1.2566760917373548E-4	0.0	0.0	15.328432296575558	0.0
44-45	1.2566760917373548E-4	0.0	0.0	16.981024191014765	0.0
46-47	1.2566760917373548E-4	0.0	0.0	18.854099905749294	0.0
48-49	1.8850141376060322E-4	0.0	0.0	20.343700911090167	0.0
50-51	2.5133521834747095E-4	0.0	0.0	21.791894439208292	0.0
52-53	2.5133521834747095E-4	0.0	0.0	23.0865849827207	0.0
54-55	2.5133521834747095E-4	0.0	0.0	24.226138862708137	0.0
56-57	2.5133521834747095E-4	0.0	0.0	25.584291548853283	0.0
58-59	2.5133521834747095E-4	0.0	0.0	26.55061262959472	0.0
60-61	2.5133521834747095E-4	0.0	0.0	27.446497015394282	0.0
62-63	2.5133521834747095E-4	0.0	0.0	28.305686459315112	0.0
64-65	2.5133521834747095E-4	0.0	0.0	29.052843229657555	0.0
66-67	2.5133521834747095E-4	0.0	0.0	29.892617027961045	0.0
68-69	2.5133521834747095E-4	0.0	0.0	30.82079798931825	0.0
70-71	2.5133521834747095E-4	0.0	0.0	31.72912346842601	0.0
72-73	2.5133521834747095E-4	0.0	0.0	32.45007854225574	0.0
74-75	2.5133521834747095E-4	0.0	0.0	33.235563933396165	0.0
76-77	2.5133521834747095E-4	0.0	0.0	33.86094879044926	0.0
78-79	2.5133521834747095E-4	6.283380458686774E-5	0.0	34.89048067860509	0.0
80-81	2.5133521834747095E-4	1.2566760917373548E-4	0.0	35.841721646245674	0.0
82-83	2.5133521834747095E-4	1.2566760917373548E-4	0.0	36.56581841030474	0.0
84-85	2.5133521834747095E-4	1.2566760917373548E-4	0.0	37.30461828463714	0.0
86-87	2.5133521834747095E-4	1.2566760917373548E-4	0.0	38.028212378259504	0.0
88-89	2.5133521834747095E-4	1.2566760917373548E-4	0.0	38.82664153314484	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCCA	520	0.0	46.683163	1
TACCCAT	575	0.0	42.17525	2
ACCCATG	585	0.0	41.417774	3
GACATAT	170	0.0	36.398865	1
AGTACTC	1175	0.0	32.750626	5
GTACCTA	75	3.04042E-4	31.732346	1
AAGTACT	1240	0.0	31.416992	4
AAAAAGT	1250	0.0	31.224627	1
AAAGTAC	1260	0.0	30.918312	3
CATATAG	200	0.0	30.880606	3
CTTTGCG	320	0.0	28.23313	2
TTTTGTA	355	0.0	28.128485	2
TTTGTAC	360	0.0	27.713366	3
GCTTTGC	330	0.0	25.96283	1
GGGCTTG	1915	0.0	25.797781	7
CGCAGAA	450	0.0	25.334743	9
ATGGGCA	550	0.0	25.050001	5
AGGATAT	250	1.4551915E-11	24.701376	8
CGTATCC	175	0.0	24.438393	94-95
ACATATA	275	1.8189894E-12	24.207542	2
>>END_MODULE