##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031651_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 795750 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8074068488847 31.0 31.0 34.0 30.0 34.0 2 32.02677851083883 33.0 31.0 34.0 30.0 34.0 3 32.0818699340245 34.0 31.0 34.0 30.0 34.0 4 35.62123531259818 37.0 35.0 37.0 33.0 37.0 5 35.32467357838517 37.0 35.0 37.0 33.0 37.0 6 35.58573169965442 37.0 35.0 37.0 33.0 37.0 7 35.52250204209865 37.0 35.0 37.0 33.0 37.0 8 35.621636192271446 37.0 35.0 37.0 33.0 37.0 9 37.43293873704053 39.0 37.0 39.0 34.0 39.0 10-11 37.29716933710336 39.0 37.0 39.0 34.0 39.0 12-13 37.30475966069746 39.0 37.0 39.0 34.0 39.0 14-15 38.13057304429783 40.0 37.0 41.0 33.0 41.0 16-17 38.094451146716935 40.0 37.5 41.0 33.0 41.0 18-19 38.39271630537229 40.0 38.0 41.0 33.5 41.0 20-21 38.5076877159912 40.0 38.0 41.0 34.0 41.0 22-23 38.39442098649073 40.0 38.0 41.0 34.0 41.0 24-25 38.07490355010996 40.0 37.5 41.0 32.5 41.0 26-27 37.655896952560475 39.5 36.5 41.0 32.0 41.0 28-29 37.571964184731385 39.5 37.0 41.0 32.0 41.0 30-31 37.29155325164939 39.0 36.5 41.0 31.5 41.0 32-33 37.070049638705626 39.0 36.0 41.0 30.5 41.0 34-35 36.819609802073515 39.0 35.0 40.5 30.0 41.0 36-37 36.37563870562363 39.0 35.0 40.0 30.0 41.0 38-39 35.9007590323594 38.0 35.0 40.0 28.0 41.0 40-41 36.064752120640904 39.0 35.0 40.0 27.5 41.0 42-43 35.99066603832863 39.0 35.0 40.0 27.5 41.0 44-45 35.942415331448316 38.5 35.0 40.5 27.5 41.0 46-47 36.029947847942196 39.0 35.0 41.0 27.5 41.0 48-49 36.25132704995288 39.5 36.0 41.0 28.0 41.0 50-51 35.766361294376374 39.0 35.0 41.0 26.0 41.0 52-53 35.93359786365065 39.0 36.0 41.0 27.5 41.0 54-55 35.90817719132893 39.0 35.5 41.0 27.0 41.0 56-57 35.40473955387999 38.5 34.5 40.5 25.5 41.0 58-59 34.878291548853284 38.0 34.0 40.0 23.5 41.0 60-61 33.9190562362551 37.0 32.0 40.0 20.0 41.0 62-63 33.58914294690544 37.0 32.0 40.0 18.5 41.0 64-65 32.85786113729186 36.5 31.0 39.5 14.5 41.0 66-67 31.486419101476592 36.0 28.0 39.0 10.5 40.5 68-69 30.066704366949416 35.0 24.5 38.0 8.0 39.5 70-71 28.824158969525605 34.5 21.5 37.0 7.0 39.0 72-73 28.21807037386114 34.0 20.0 36.5 7.0 38.5 74-75 27.90960289035501 34.0 20.0 35.5 7.0 37.0 76-77 27.47512723845429 33.0 20.0 35.0 7.0 36.5 78-79 27.480694313540685 33.0 20.0 35.0 7.0 36.0 80-81 27.24496135721018 33.0 20.0 35.0 7.0 36.0 82-83 26.984558592522777 33.0 19.5 35.0 7.0 35.5 84-85 26.828290920515236 33.0 19.0 35.0 7.0 35.0 86-87 26.88567012252592 33.0 19.0 35.0 7.0 35.0 88-89 26.690717562048384 33.0 19.0 35.0 7.0 35.0 90-91 26.52927929626139 33.0 19.0 35.0 7.0 35.0 92-93 26.449057492931196 33.0 19.0 35.0 7.0 35.0 94-95 26.33890983349042 33.0 18.0 35.0 7.0 35.0 96-97 26.212324850769715 32.5 18.0 35.0 7.0 35.0 98-99 26.23008482563619 33.0 18.0 35.0 7.0 35.0 100-101 25.70768206094879 32.0 16.0 34.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.03602391258935356 1101 2 0.09044465753692066 1101 3 0.006980196653259441 1101 4 0.06129709174196307 1101 5 0.0687237595448309 1101 6 0.11365453620324928 1101 7 0.346077188646575 1101 8 0.09945696431450557 1101 9 0.17475266699089786 1101 10-11 -0.14489769163634492 1101 12-13 0.008020359200884286 1101 14-15 0.11891548139616503 1101 16-17 0.12421711684389436 1101 18-19 0.10079110649354561 1101 20-21 0.11442584265451217 1101 22-23 0.348421256017275 1101 24-25 0.1251105328880584 1101 26-27 0.2533749950714608 1101 28-29 0.2693112890840723 1101 30-31 -0.017440254067871308 1101 32-33 -0.04966533060036227 1101 34-35 0.03828980242482771 1101 36-37 0.005046766206412201 1101 38-39 0.24678295087168323 1101 40-41 0.02557277503222366 1101 42-43 -0.0957279664831816 1101 44-45 0.06299396140400404 1101 46-47 -0.013337959119247955 1101 48-49 -0.07191413513582035 1101 50-51 0.2263657545083575 1101 52-53 -0.08189002819501923 1101 54-55 0.15367967477937583 1101 56-57 0.17178928988044362 1101 58-59 -0.11157799505299693 1101 60-61 -0.43172372526703384 1101 62-63 -0.027605548816211467 1101 64-65 0.021394281405299864 1101 66-67 -0.11815672686831391 1101 68-69 -0.1187154024169601 1101 70-71 0.180495696966414 1101 72-73 0.29638450381984427 1101 74-75 0.25972263699399534 1101 76-77 0.21421210219009978 1101 78-79 0.4856528661058981 1101 80-81 0.07168877543308128 1101 82-83 -0.17034865361640428 1101 84-85 0.1310518403455596 1101 86-87 0.06962072948730125 1101 88-89 0.06612159777881743 1101 90-91 -0.2170204243279663 1101 92-93 -0.4397145929493469 1101 94-95 -0.21008285988752107 1101 96-97 -0.2114903170952651 1101 98-99 -0.3528875908127773 1101 100-101 -0.2674340557071524 1102 1 0.18278054698014046 1102 2 0.27007483977043734 1102 3 0.24922163805761954 1102 4 0.1961534966687566 1102 5 0.026126288793776098 1102 6 0.18774671281850175 1102 7 0.15834630978916664 1102 8 0.15866227800106003 1102 9 0.24674828350818956 1102 10-11 0.297619260917088 1102 12-13 0.2483697515843275 1102 14-15 0.2745715632211798 1102 16-17 0.05011336412994183 1102 18-19 0.20605771690894414 1102 20-21 0.13675742328074847 1102 22-23 0.09679288480008097 1102 24-25 0.1966289568922548 1102 26-27 0.22351380535720722 1102 28-29 -0.18143626964533155 1102 30-31 -0.4128669233413049 1102 32-33 0.08483993266585088 1102 34-35 0.13415790692869933 1102 36-37 0.26895480567833374 1102 38-39 0.2398270017859403 1102 40-41 0.09778833742482362 1102 42-43 0.34021247577523894 1102 44-45 0.33298845632596397 1102 46-47 0.11528407354078496 1102 48-49 0.2212775753485161 1102 50-51 0.4790179673439283 1102 52-53 0.23791256073514688 1102 54-55 0.17048688385915511 1102 56-57 -0.12358644455870404 1102 58-59 0.029642691692622236 1102 60-61 -0.05138199883339922 1102 62-63 -0.1541335938446622 1102 64-65 -0.1788349253489585 1102 66-67 -0.5334235430810281 1102 68-69 -0.6095175488829057 1102 70-71 -0.4436412405638137 1102 72-73 -0.17651327665616634 1102 74-75 -0.21655021936977548 1102 76-77 -0.3175411365450742 1102 78-79 -0.5459974739741575 1102 80-81 -0.5908280742216938 1102 82-83 -1.2289224268071912 1102 84-85 -0.7680508833818429 1102 86-87 -0.4060193001475092 1102 88-89 -0.45249019646503186 1102 90-91 -0.4964481534575462 1102 92-93 -0.47942189772487254 1102 94-95 -0.5623113875106647 1102 96-97 -0.5306883764266281 1102 98-99 -0.1851293409131074 1102 100-101 -0.14194528709536058 1103 1 0.16368733634701726 1103 2 0.15671109928941718 1103 3 0.2822894111693941 1103 4 0.11253216612162475 1103 5 0.1708499119326774 1103 6 0.12976885511591263 1103 7 0.3332091762321312 1103 8 0.2282186283396186 1103 9 0.30938265430125256 1103 10-11 0.29490421741611783 1103 12-13 0.2523405968896384 1103 14-15 0.2883477090147437 1103 16-17 0.2729101854444167 1103 18-19 0.45543735081760417 1103 20-21 0.1862561072004567 1103 22-23 0.022389139496780786 1103 24-25 -0.090153477956747 1103 26-27 0.23825189533290114 1103 28-29 0.6195492836107093 1103 30-31 0.3836429522840845 1103 32-33 0.48442853199475167 1103 34-35 0.5535505007119852 1103 36-37 0.28063627406226743 1103 38-39 0.4208935291423259 1103 40-41 0.6818592450178969 1103 42-43 0.4102410075922336 1103 44-45 0.6682950728945087 1103 46-47 0.385911873978813 1103 48-49 0.11479562875526028 1103 50-51 0.4131838135534238 1103 52-53 0.7081626159904246 1103 54-55 0.44930251206144334 1103 56-57 0.4830512214582896 1103 58-59 0.9591017927394603 1103 60-61 0.8612694508642065 1103 62-63 0.8287046186972873 1103 64-65 0.8530348128414786 1103 66-67 0.7457035239687428 1103 68-69 0.7077205913248541 1103 70-71 0.6012159998870672 1103 72-73 0.28487081786050084 1103 74-75 0.4100923693902949 1103 76-77 0.47645964707525224 1103 78-79 0.7154730197066499 1103 80-81 0.6985011266865016 1103 82-83 0.5904706692968098 1103 84-85 0.1785373542761377 1103 86-87 0.3046314061154227 1103 88-89 0.47598435596720634 1103 90-91 0.6915478882551689 1103 92-93 0.3375299196714998 1103 94-95 0.3884988558134914 1103 96-97 0.38405021586978094 1103 98-99 0.47637681753894157 1103 100-101 0.4996714823796147 1104 1 0.0010855189356533401 1104 2 0.17886845133047302 1104 3 0.2040795142815952 1104 4 0.14971847432822472 1104 5 0.15938515370488915 1104 6 0.10967845537957999 1104 7 0.310646908705408 1104 8 0.1880519538257559 1104 9 0.4735711754180656 1104 10-11 0.10127692799571264 1104 12-13 0.06422439556188664 1104 14-15 0.1807484811791582 1104 16-17 0.3005197715831258 1104 18-19 0.14523206808301836 1104 20-21 0.15642184005040605 1104 22-23 0.11589165844777227 1104 24-25 0.302986500384975 1104 26-27 0.21516700433050318 1104 28-29 0.07792773834022171 1104 30-31 0.03177610356239313 1104 32-33 0.3428611323996691 1104 34-35 0.2741046699289882 1104 36-37 0.08215235297370072 1104 38-39 0.2511303576182371 1104 40-41 0.09048488652644693 1104 42-43 0.2346701238406439 1104 44-45 0.14730302995462807 1104 46-47 0.26684553375563524 1104 48-49 0.26371506940689216 1104 50-51 0.6205523337285754 1104 52-53 0.5333897924111639 1104 54-55 0.3792686121603239 1104 56-57 0.2118731998941925 1104 58-59 0.21313446847749162 1104 60-61 0.43122566349192226 1104 62-63 0.09935295430631186 1104 64-65 -0.3722111968576627 1104 66-67 -0.4546684332797142 1104 68-69 -0.26488640523807305 1104 70-71 0.022144480512334752 1104 72-73 0.2938189792616974 1104 74-75 -0.15902811650828497 1104 76-77 -0.2357866922062577 1104 78-79 -0.0424063531451786 1104 80-81 0.056251020749307656 1104 82-83 0.39490826052050565 1104 84-85 0.3968126968063892 1104 86-87 0.25572373626279443 1104 88-89 0.33045019710850454 1104 90-91 -0.15416989506796597 1104 92-93 -0.3598216505530871 1104 94-95 -0.009033984715721033 1104 96-97 -0.1506736411856373 1104 98-99 0.010787571340912905 1104 100-101 -0.06508674540888748 1105 1 0.20584433355818987 1105 2 0.20290046517429516 1105 3 0.15932502585416586 1105 4 0.2123401485657368 1105 5 0.2026471048188796 1105 6 0.1788110532597429 1105 7 0.19059499436407634 1105 8 0.2562760507325237 1105 9 0.2578670871636888 1105 10-11 0.1882658961981747 1105 12-13 0.286071680874457 1105 14-15 0.2780770556978709 1105 16-17 0.27636878737374104 1105 18-19 0.16152019267743611 1105 20-21 0.1903067632604447 1105 22-23 0.02610147891067527 1105 24-25 0.27371979045636863 1105 26-27 0.33011400844038263 1105 28-29 0.3495154519461039 1105 30-31 0.14443022426323893 1105 32-33 0.5123830878398579 1105 34-35 0.29345357756472623 1105 36-37 0.7148908344792204 1105 38-39 0.35947991418323966 1105 40-41 0.458817138310998 1105 42-43 0.39496646820577297 1105 44-45 0.6419818099676107 1105 46-47 0.718868029970217 1105 48-49 0.5769315954722174 1105 50-51 0.6537018037696711 1105 52-53 0.5370886892314033 1105 54-55 0.6530194233219078 1105 56-57 0.2480834529766085 1105 58-59 0.2961108907141323 1105 60-61 0.4245849539959181 1105 62-63 0.08171289373957791 1105 64-65 0.17496596083214655 1105 66-67 0.16027857083017238 1105 68-69 0.11777636785299705 1105 70-71 0.22960716707950013 1105 72-73 0.17640604393831438 1105 74-75 0.2117268045386531 1105 76-77 0.11013586890124927 1105 78-79 0.268225271341084 1105 80-81 0.04176908261390011 1105 82-83 -0.1318925181946149 1105 84-85 -0.10029956795674977 1105 86-87 0.09041982105621926 1105 88-89 0.3389295395268448 1105 90-91 0.20904663099735998 1105 92-93 0.10312448831777843 1105 94-95 -0.2708985748087329 1105 96-97 0.02161707912584987 1105 98-99 -0.26858729346549026 1105 100-101 -0.023425486486111424 1106 1 0.03532112058840653 1106 2 0.15147575380116507 1106 3 0.1190892115579274 1106 4 0.29943177224541984 1106 5 0.1547473258947818 1106 6 -0.16174409289599367 1106 7 0.08326264359030944 1106 8 0.10873244965171125 1106 9 0.21680837922951923 1106 10-11 0.13007994679999513 1106 12-13 0.1785489591844538 1106 14-15 0.10101368060575311 1106 16-17 0.4658983869807116 1106 18-19 0.2619746264774818 1106 20-21 0.32333304672888374 1106 22-23 0.4184129502936287 1106 24-25 0.2696974372261991 1106 26-27 0.32956745968086665 1106 28-29 0.37905978994659506 1106 30-31 0.0595862055946057 1106 32-33 0.14843074197888484 1106 34-35 0.0128284240397889 1106 36-37 0.21939832649592717 1106 38-39 0.1966759348169944 1106 40-41 0.4099532228111187 1106 42-43 0.47082620782748563 1106 44-45 0.36117856752103705 1106 46-47 0.16077419553150918 1106 48-49 0.37434623560977087 1106 50-51 0.35163842867755335 1106 52-53 0.23981529203170027 1106 54-55 0.2393495633366456 1106 56-57 0.21578794818662317 1106 58-59 0.264380357675563 1106 60-61 0.31765790904381674 1106 62-63 0.4024940285958749 1106 64-65 0.5813894440058149 1106 66-67 0.6898167678333635 1106 68-69 0.45223350369185766 1106 70-71 0.402206037135997 1106 72-73 0.36948513998351373 1106 74-75 0.26210140537383 1106 76-77 0.3989653948162548 1106 78-79 0.5968423187495837 1106 80-81 0.42442076524715944 1106 82-83 0.1353943891991456 1106 84-85 0.15378419524639142 1106 86-87 0.19313414186191835 1106 88-89 0.39925869698201666 1106 90-91 0.3635234180275724 1106 92-93 0.31549245687586946 1106 94-95 0.2851687305216508 1106 96-97 0.243921181312043 1106 98-99 0.31854117619798217 1106 100-101 0.37731143318964655 1107 1 2.296583782985806E-4 1107 2 0.1383925093619922 1107 3 0.10585385933951841 1107 4 0.12240399776001709 1107 5 0.0575168727321298 1107 6 0.11783000532217613 1107 7 -0.03184343677001067 1107 8 0.06486022018626159 1107 9 -0.05283525397343425 1107 10-11 0.1522215058059544 1107 12-13 0.19662706751680048 1107 14-15 0.20309479158517263 1107 16-17 0.11014298419141255 1107 18-19 -0.09877168935357616 1107 20-21 0.08751462785961195 1107 22-23 0.09680179308924863 1107 24-25 0.012166126728786253 1107 26-27 0.1612582265411291 1107 28-29 0.09018637522571993 1107 30-31 0.12778382718205705 1107 32-33 0.3368231405407798 1107 34-35 0.25160701832685817 1107 36-37 0.20900758729163016 1107 38-39 0.40422502414574524 1107 40-41 0.48885666400270367 1107 42-43 0.5269569415005932 1107 44-45 0.4207139473953987 1107 46-47 0.4777055692425378 1107 48-49 0.30150340047880064 1107 50-51 0.42487338581081957 1107 52-53 0.4040087257166576 1107 54-55 0.5976226461859966 1107 56-57 0.6842038543143971 1107 58-59 0.6135832734039042 1107 60-61 0.7758974096562028 1107 62-63 0.8734631724463782 1107 64-65 0.5739322119723127 1107 66-67 0.7854787329868529 1107 68-69 0.8154757699002246 1107 70-71 1.0897470272193601 1107 72-73 0.9199957875280589 1107 74-75 0.9799187575132144 1107 76-77 0.9544857219467566 1107 78-79 1.1035804958267441 1107 80-81 1.053302476755988 1107 82-83 0.7531485277159966 1107 84-85 0.9504605414990088 1107 86-87 0.46707432597159126 1107 88-89 0.606903594457421 1107 90-91 0.6664855688710816 1107 92-93 0.8013306300021803 1107 94-95 0.9118824123200753 1107 96-97 0.6101394263438458 1107 98-99 0.8714806096458929 1107 100-101 1.1460029112031584 1108 1 -0.21842521615892707 1108 2 -0.2479094239987667 1108 3 -0.23032466801547713 1108 4 0.027278534987495107 1108 5 -0.22107218526869588 1108 6 -0.09321483219324733 1108 7 -0.33060937671241675 1108 8 -0.2277354194682033 1108 9 -0.0724979442243594 1108 10-11 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-0.7715837574473383 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 7.0 13 98.0 14 383.0 15 1023.0 16 2243.0 17 3957.0 18 5833.0 19 7835.0 20 9317.0 21 10422.0 22 10884.0 23 12388.0 24 15669.0 25 21212.0 26 28172.0 27 35917.0 28 47180.0 29 32372.0 30 17701.0 31 18570.0 32 22313.0 33 28392.0 34 37143.0 35 52719.0 36 89008.0 37 156401.0 38 118294.0 39 10275.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.834832165572664 20.82218062175241 17.58480356350351 19.75818364917142 2 12.284917184004826 25.793852263302085 42.0173674818408 19.90386307085229 3 22.259587532218156 30.27180749660382 38.04777146505108 9.420833506126948 4 8.725027900383065 24.38129015392968 36.14544896994802 30.74823297573924 5 6.171033616085454 43.96757775683318 35.629908890983344 14.231479736098022 6 17.717624882186616 43.01539428212378 14.664907320138235 24.602073515551368 7 16.75186930568646 36.64392082940622 17.910650329877473 28.693559535029845 8 21.155890669180017 30.118630223060006 22.542758404021363 26.18272070373861 9 18.585234055922086 19.631793905120958 18.417970468111843 43.365001570845116 10-11 22.220169651272386 23.75142946905435 28.21363493559535 25.814765944077916 12-13 31.640841972981466 20.753879987433237 23.90807414388941 23.697203895695885 14-15 24.71127866792334 25.038642789820926 26.432233741753063 23.817844800502673 16-17 20.379893182532204 30.95545083254791 25.739742381401193 22.924913603518693 18-19 20.187056236255106 31.38152686145146 23.109582155199497 25.321834747093934 20-21 22.77932767829092 23.36449890040842 21.236003770028276 32.620169651272384 22-23 20.732935669883624 31.041060960889443 20.764227632059306 27.461775737167628 24-25 28.790826264530317 31.49035501099592 20.66120012566761 19.057618598806158 26-27 27.280929940307885 25.023185673892556 30.20722588752749 17.48865849827207 28-29 22.95567740820463 30.025672851823337 25.657077317077004 21.36157242289503 30-31 21.41470968260911 28.93533531419838 27.98851701583184 21.661437987360674 32-33 24.83166823751178 30.366195413132264 26.523845428840715 18.278290920515236 34-35 28.589443920829403 24.18554822494502 28.299780081683945 18.92522777254163 36-37 19.711781338360037 27.925039271127865 31.307194470625195 21.0559849198869 38-39 19.167012252591896 22.419541313226514 32.68532830662897 25.728118127552623 40-41 16.66082312284009 24.105183788878417 33.778887841658815 25.455105246622683 42-43 21.12177191328935 23.867232170907947 31.996921143575243 23.01407477222746 44-45 17.745900094250707 23.341564561734213 31.321520578071006 27.591014765944077 46-47 25.192334275840402 22.57348413446434 27.56418473138548 24.66999685830977 48-49 20.917275728296918 22.484368046703523 30.130259992004888 26.46809623299467 50-51 12.472004444098122 24.172952836757318 36.55993374070899 26.795108978435568 52-53 16.07521206409048 26.827961043041153 33.692491360351866 23.404335532516495 54-55 13.945807982336039 22.95221644635935 30.235081737919643 32.866893833384964 56-57 22.994847628023876 22.65830977065661 25.882563619227145 28.464278982092367 58-59 21.240402136349356 27.070813697769402 29.497455230914234 22.19132893496701 60-61 20.1961169941043 35.17262656149956 24.142459404624194 20.48879703977194 62-63 15.323656927426956 39.4052780395853 22.069117185045556 23.201947847942193 64-65 14.427458372604463 43.73879987433239 18.753942821237825 23.07979893182532 66-67 17.600314169022933 37.9851712221175 18.264278982092367 26.1502356267672 68-69 18.136726358781026 37.06446748350613 19.848633364750235 24.950172792962615 70-71 21.829783223374175 36.55601633678919 19.27803958529689 22.33616085453974 72-73 20.43009739239711 32.20263901979265 24.421237825950364 22.946025761859882 74-75 18.807037386113727 37.60138234370091 21.042412818096135 22.549167452089225 76-77 21.422934338674207 32.36676091737355 21.254476908576812 24.955827835375434 78-79 21.794651070439386 32.04921658615542 22.8701800067358 23.285952336669396 80-81 23.301912877359676 30.196440690747306 23.355453334338787 23.14619309755423 82-83 21.05707317962088 29.54644708821657 26.656059740620442 22.740419991542108 84-85 20.33415017279296 30.042664153314487 25.575620483820295 24.04756519007226 86-87 20.699214577442664 29.568708765315737 24.407288721332076 25.32478793590952 88-89 20.68143261074458 29.0770342444235 23.35425699026076 26.88727615457116 90-91 22.374238140119385 25.107068803016023 25.319886899151744 27.198806157712852 92-93 22.4980835689601 27.32761545711593 25.826390197926486 24.347910775997487 94-95 21.608482563619226 26.67452089224003 26.73741753063148 24.979579013509266 96-97 17.876531573986803 27.421363493559536 29.229406220546654 25.472698711907004 98-99 20.52214891611687 29.037574615142947 24.777442664153316 25.662833804586864 100-101 19.761420043983662 28.839019792648447 25.289035501099594 26.110524662268304 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 148.0 1 89.5 2 51.5 3 122.0 4 100.0 5 41.5 6 79.0 7 131.5 8 160.5 9 154.0 10 115.5 11 79.0 12 71.0 13 58.5 14 66.5 15 80.5 16 82.5 17 84.0 18 90.0 19 110.0 20 128.5 21 156.0 22 189.5 23 237.5 24 318.5 25 412.5 26 527.0 27 687.5 28 884.5 29 1090.0 30 1414.0 31 1959.5 32 2950.0 33 4868.0 34 8092.0 35 11107.5 36 14411.0 37 18261.0 38 23368.0 39 28948.5 40 30587.0 41 30370.0 42 30551.5 43 29351.5 44 27644.0 45 27605.0 46 33730.5 47 63060.0 48 111258.5 49 118898.0 50 87640.5 51 53808.5 52 21111.5 53 4877.5 54 1347.5 55 605.0 56 333.5 57 203.0 58 144.0 59 115.5 60 95.0 61 76.5 62 62.0 63 50.0 64 43.5 65 45.0 66 32.5 67 30.0 68 35.0 69 30.5 70 27.5 71 26.0 72 24.0 73 18.5 74 12.0 75 13.0 76 12.5 77 6.0 78 4.0 79 4.0 80 3.0 81 1.0 82 0.5 83 1.0 84 0.5 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044360666038328625 2 0.0012566760917373547 3 3.7700282752120643E-4 4 0.007791391768771599 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.002324850769714106 24-25 0.0 26-27 0.0 28-29 0.020420986490732013 30-31 0.017342130065975497 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.033176248821866164 50-51 0.01105874960728872 52-53 0.0 54-55 6.91171850455545E-4 56-57 0.0 58-59 0.0 60-61 6.283380458686773E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.513352183474709E-4 80-81 0.011310084825636191 82-83 0.005466540999057493 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 795750.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.914629722484452 #Duplication Level Percentage of deduplicated Percentage of total 1 74.64917152107945 12.626630953692905 2 10.781717626204202 3.6473752283925625 3 4.292711920892353 2.178288979415674 4 2.3111600958255285 1.5636966900108198 5 1.39241113320379 1.1776059369803544 6 0.9912373289879981 1.0059847432161897 7 0.6719981755492621 0.7956620219520611 8 0.47796120577090007 0.646762945386158 9 0.4041092259409172 0.6151822131807382 >10 3.130612906958574 10.784072921245478 >50 0.4301882061361074 5.13451729248856 >100 0.39365575882125603 13.591999847339236 >500 0.03131352626987263 3.5673639977874587 >1k 0.028331285672741912 10.204214693331801 >5k 0.008946721791392182 11.066294030466809 >10k+ 0.004473360895696091 21.394347505113206 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 59160 7.434495758718191 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 39865 5.009739239710965 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 29963 3.7653785736726357 TruSeq Adapter, Index 3 (95% over 21bp) GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 16718 2.1009110901665093 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 11980 1.5054979579013508 No Hit TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG 11964 1.5034872761545712 TruSeq Adapter, Index 3 (96% over 28bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9803 1.2319195727301289 No Hit ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 9237 1.1607917059377946 RNA PCR Primer, Index 3 (95% over 23bp) GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9067 1.1394282123782595 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8518 1.0704366949418787 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7589 0.9536914860194784 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7477 0.9396167137920202 No Hit TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC 6581 0.8270185359723532 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6484 0.8148287778825009 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6117 0.7687087653157398 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6007 0.7548853283066289 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5603 0.7041156142004399 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5269 0.6621426327364122 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4783 0.6010681746779768 No Hit TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT 4661 0.585736726358781 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4470 0.5617342130065975 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4445 0.5585925227772541 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4137 0.5198868991517437 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3528 0.44335532516493875 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3522 0.44260131950989634 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3213 0.403770028275212 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2972 0.3734841344643418 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2656 0.3337731699654414 No Hit CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT 2642 0.33201382343700914 TruSeq Adapter, Index 3 (96% over 26bp) GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2343 0.29443920829406217 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2338 0.2938108702481935 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2221 0.2791077599748665 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2200 0.276468740182218 No Hit CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA 1877 0.2358781024191015 RNA PCR Primer, Index 3 (96% over 28bp) CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1858 0.2334904178448005 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1838 0.23097706566132578 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1787 0.2245680175934653 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATATCGTATGCCGT 1664 0.20911090166509583 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC 1610 0.2023248507697141 No Hit ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC 1587 0.1994344957587182 Illumina PCR Primer Index 12 (95% over 24bp) CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1510 0.18975808985234058 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1390 0.1746779767514923 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1271 0.15972353125981778 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1226 0.1540684888469997 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1226 0.1540684888469997 No Hit CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG 1200 0.15080113100848258 TruSeq Adapter, Index 3 (95% over 24bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1176 0.1477851083883129 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1147 0.1441407477222746 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 1110 0.13949104618284638 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1085 0.13634935595350298 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1083 0.1360980207351555 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1045 0.13132265158655357 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 1041 0.13081998114985863 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1027 0.12906063462142633 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1021 0.12830662896638392 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAGG 1006 0.1264216148287779 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 968 0.12164624568017593 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 956 0.12013823437009111 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 945 0.11875589066918002 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 901 0.11322651586553566 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 887 0.11146716933710335 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 858 0.10782280867106503 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 852 0.10706880301602263 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 841 0.10568645931511153 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 833 0.10468111844172165 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 827 0.10392711278667922 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.7700282752120643E-4 0.0 2 0.0 0.0 0.0 0.0015080113100848257 0.0 3 0.0 0.0 0.0 0.0017593465284322966 0.0 4 0.0 0.0 0.0 0.00276468740182218 0.0 5 0.0 0.0 0.0 0.0030160226201696515 0.0 6 0.0 0.0 0.0 0.0031416902293433867 0.0 7 0.0 0.0 0.0 0.003644360666038329 0.0 8 0.0 0.0 0.0 0.004398366321080742 0.0 9 0.0 0.0 0.0 0.008419729814640276 0.0 10-11 0.0 0.0 0.0 0.012378259503612944 0.0 12-13 0.0 0.0 0.0 0.01834747093936538 0.0 14-15 0.0 0.0 0.0 0.025887527489789508 0.0 16-17 0.0 0.0 0.0 0.04184731385485391 0.0 18-19 0.0 0.0 0.0 0.057681432610744585 0.0 20-21 0.0 0.0 0.0 0.08972667295004713 0.0 22-23 0.0 0.0 0.0 0.1976751492302859 0.0 24-25 0.0 0.0 0.0 0.4598177819666981 0.0 26-27 0.0 0.0 0.0 0.8668551680804273 0.0 28-29 0.0 0.0 0.0 1.4732642161482876 0.0 30-31 0.0 0.0 0.0 2.429971724787936 0.0 32-33 0.0 0.0 0.0 3.7820923656927428 0.0 34-35 0.0 0.0 0.0 5.870185359723531 0.0 36-37 0.0 0.0 0.0 7.9646874018221805 0.0 38-39 0.0 0.0 0.0 10.430097392397109 0.0 40-41 0.0 0.0 0.0 13.383223374175305 0.0 42-43 0.0 0.0 0.0 15.19396795475966 0.0 44-45 0.0 0.0 0.0 16.84090480678605 0.0 46-47 0.0 0.0 0.0 18.699591580270187 0.0 48-49 0.0 0.0 0.0 20.17392397109645 0.0 50-51 0.0 0.0 0.0 21.606157712849516 0.0 52-53 0.0 0.0 0.0 22.889852340559223 0.0 54-55 0.0 0.0 0.0 24.01702796104304 0.0 56-57 0.0 0.0 0.0 25.360917373546968 0.0 58-59 0.0 0.0 0.0 26.317247879359094 0.0 60-61 0.0 0.0 0.0 27.197926484448633 0.0 62-63 0.0 0.0 0.0 28.044423499842914 0.0 64-65 0.0 0.0 0.0 28.784480050267042 0.0 66-67 0.0 0.0 0.0 29.613572101790762 0.0 68-69 0.0 0.0 0.0 30.527678290920512 0.0 70-71 0.0 0.0 0.0 31.42808671065033 0.0 72-73 0.0 0.0 0.0 32.14326107445806 0.0 74-75 0.0 0.0 0.0 32.92409676405906 0.0 76-77 0.0 0.0 0.0 33.54539742381401 0.0 78-79 0.0 0.0 0.0 34.568771599120325 0.0 80-81 0.0 0.0 6.283380458686774E-5 35.51957273012881 0.0 82-83 0.0 0.0 1.2566760917373548E-4 36.2420986490732 0.0 84-85 0.0 0.0 1.2566760917373548E-4 36.97807100219919 0.0 86-87 0.0 0.0 1.2566760917373548E-4 37.6951932139491 0.0 88-89 0.0 0.0 1.2566760917373548E-4 38.48903550109959 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACATAT 95 0.0 60.03431 1 CGGAGAT 80 1.8189894E-12 59.408955 1 GGAGCTT 40 7.548704E-6 59.36789 8 GTACCAG 40 5.3720106E-4 47.52716 1 ATAGTCG 20 5.3919904E-4 47.494312 90-91 TAGGATA 120 0.0 47.494312 7 GTACCCA 390 0.0 46.308517 1 CATATAG 125 0.0 45.59454 3 ACCCATG 415 0.0 44.633213 3 CTAGATA 55 4.9654354E-5 43.176647 4 TAATCTA 55 4.9654354E-5 43.176647 5 GGATATG 140 0.0 40.70941 9 GGGAGCT 60 8.294299E-5 39.578594 7 TCGCCGA 50 0.0016164674 37.995453 8 TGTTCGT 25 0.0016169534 37.995453 92-93 TACCCAT 490 0.0 36.834637 2 TATATTG 65 1.3287533E-4 36.53409 5 TAGATAG 65 1.3287533E-4 36.53409 5 AGTACTC 1025 0.0 35.678654 5 AAGTACT 1030 0.0 35.50546 4 >>END_MODULE