FastQCFastQC Report
Fri 10 Feb 2017
SRR2031650_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031650_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604622
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7850.037.6138461
GGTATCA6050.033.848451
TACCGTA1308.223651E-932.9048467
GTACATG20950.031.3704531
TACATGG20700.030.3361552
TCAACGC9100.030.3008884
CAACGCA9400.029.333845
TATCAAC10050.028.8749162
ACATGGG21650.028.544223
ATCAACG9750.028.2784823
GGACCGA1205.350461E-627.725386
AACGCAG9950.027.7054776
ACCGTAT1554.5525667E-827.5976168
TACACCG1109.413861E-525.931485
CATGGGG14350.025.8411274
CCGTATG1501.0530512E-625.348929
GTAGGAC2501.4551915E-1124.7192963
TGTACCG1951.5523256E-824.3800265
TAACGAT500.001637473123.7902546-47
GTACCGT1801.936296E-723.7646126
Produced by FastQC (version 0.11.5)