##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031650_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 604622 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92021295950197 33.0 31.0 34.0 30.0 34.0 2 32.170270019946344 34.0 31.0 34.0 30.0 34.0 3 32.33559314745411 34.0 31.0 34.0 30.0 34.0 4 35.421086232389825 37.0 35.0 37.0 33.0 37.0 5 35.10300650654459 37.0 35.0 37.0 32.0 37.0 6 35.31572122747767 37.0 35.0 37.0 32.0 37.0 7 35.46246249722967 37.0 35.0 37.0 33.0 37.0 8 35.54755037031401 37.0 35.0 37.0 33.0 37.0 9 37.49652510163375 39.0 38.0 39.0 35.0 39.0 10-11 37.44321410732656 39.0 38.0 39.0 35.0 39.0 12-13 37.46321255263619 39.0 38.0 39.0 35.0 39.0 14-15 38.90059574411781 41.0 39.0 41.0 35.5 41.0 16-17 38.847731309810094 41.0 39.0 41.0 35.0 41.0 18-19 38.752053349034604 41.0 38.5 41.0 34.0 41.0 20-21 38.742359689194245 41.0 39.0 41.0 34.0 41.0 22-23 38.67488943505198 40.0 38.5 41.0 34.0 41.0 24-25 38.600028612918486 40.0 38.0 41.0 34.0 41.0 26-27 38.50474594043882 40.0 38.0 41.0 34.0 41.0 28-29 38.40084796782122 40.0 38.0 41.0 34.0 41.0 30-31 38.28650131817896 40.0 38.0 41.0 34.0 41.0 32-33 38.158966263219 40.0 38.0 41.0 33.0 41.0 34-35 38.03595304173517 40.0 38.0 41.0 33.0 41.0 36-37 37.9236729725349 40.0 38.0 41.0 33.0 41.0 38-39 37.75845817717516 40.0 38.0 41.0 32.5 41.0 40-41 37.61063441290591 40.0 37.5 41.0 32.0 41.0 42-43 37.46144285189755 40.0 37.0 41.0 31.0 41.0 44-45 37.26950557538429 40.0 37.0 41.0 31.0 41.0 46-47 37.117196364009246 40.0 37.0 41.0 31.0 41.0 48-49 37.30531968734185 40.0 37.0 41.0 31.0 41.0 50-51 37.06988829384309 39.5 36.5 40.5 31.0 41.0 52-53 37.17920121993576 39.5 36.5 40.5 31.0 41.0 54-55 37.444516573991685 40.0 37.0 41.0 32.0 41.0 56-57 37.32258667398804 40.0 36.0 41.0 31.0 41.0 58-59 37.10989097320309 39.5 36.0 41.0 31.0 41.0 60-61 36.78952055995316 39.0 35.0 41.0 31.0 41.0 62-63 36.42966845400928 39.0 35.0 41.0 30.0 41.0 64-65 36.12891194829166 38.0 35.0 41.0 30.0 41.0 66-67 35.87933700725412 37.5 35.0 40.0 29.5 41.0 68-69 35.59549685588682 37.0 35.0 40.0 29.0 41.0 70-71 35.2589163146561 36.5 35.0 39.0 29.0 41.0 72-73 34.87050669674606 36.0 34.0 39.0 29.0 41.0 74-75 34.4312950901555 35.5 34.0 38.0 28.5 40.0 76-77 34.04442114246587 35.0 34.0 37.0 28.0 39.0 78-79 33.705321672052946 35.0 34.0 37.0 27.5 39.0 80-81 33.409705568107015 35.0 34.0 36.0 27.0 38.0 82-83 33.11290194534767 35.0 33.5 36.0 26.5 37.0 84-85 32.88233970976908 35.0 33.0 35.5 26.0 37.0 86-87 32.669636566317465 35.0 33.0 35.0 26.0 36.5 88-89 32.46416273307951 35.0 33.0 35.0 25.5 36.0 90-91 32.292984707800905 35.0 33.0 35.0 25.0 36.0 92-93 32.13831120931756 35.0 33.0 35.0 25.0 35.5 94-95 31.988965006235304 35.0 32.5 35.0 25.0 35.0 96-97 31.90667557581431 35.0 32.0 35.0 25.0 35.0 98-99 31.79918775697874 35.0 32.0 35.0 24.0 35.0 100-101 31.32754018213032 34.5 31.5 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1848687212111173 1101 2 0.3225559786710974 1101 3 0.42229742119551616 1101 4 0.2539034990029876 1101 5 0.27126871827861976 1101 6 0.25309793647127776 1101 7 0.2931947668803261 1101 8 0.15833686637699884 1101 9 0.4021226972204559 1101 10-11 0.3862170783911907 1101 12-13 0.4231607460355704 1101 14-15 0.48043068521642596 1101 16-17 0.4355568692444578 1101 18-19 0.517049277080659 1101 20-21 0.24503621196980419 1101 22-23 0.3147428299713084 1101 24-25 0.5508299284628961 1101 26-27 0.4988903389947481 1101 28-29 0.4118195187143172 1101 30-31 0.3645762035547193 1101 32-33 0.478989704636561 1101 34-35 0.30409424097638293 1101 36-37 0.47540031021134865 1101 38-39 0.5633499171217764 1101 40-41 0.2740306631507181 1101 42-43 0.46363722716956346 1101 44-45 0.47155507071244784 1101 46-47 0.6185065749533152 1101 48-49 0.452989254148882 1101 50-51 0.30244749178787345 1101 52-53 0.21829369885993088 1101 54-55 0.2734755556750983 1101 56-57 0.2731862175683446 1101 58-59 0.29137423172978316 1101 60-61 0.0858563992125525 1101 62-63 0.05206773943052667 1101 64-65 0.10380579645190124 1101 66-67 -6.373678363900126E-4 1101 68-69 0.08874286679858301 1101 70-71 -0.012638072447565207 1101 72-73 0.14489077911057535 1101 74-75 0.07641016836789305 1101 76-77 0.02432050925265372 1101 78-79 0.06255189210826728 1101 80-81 0.127391925057303 1101 82-83 0.00543831104157988 1101 84-85 0.05541807747574978 1101 86-87 -0.013325697585088392 1101 88-89 -0.10530858880086669 1101 90-91 0.09211926206751997 1101 92-93 0.13473523841060597 1101 94-95 0.012675402740988773 1101 96-97 0.34596703804479034 1101 98-99 0.17714760568111032 1101 100-101 0.055585925056750085 1102 1 -0.04135578803226281 1102 2 0.0699544921073425 1102 3 -0.09819047665087055 1102 4 0.10616193239349059 1102 5 -0.07491005054166067 1102 6 0.1039460401495731 1102 7 0.12948216920546685 1102 8 0.10314342250430997 1102 9 0.31319569131231617 1102 10-11 0.3034650296014121 1102 12-13 0.2825280035160276 1102 14-15 0.28914042411484076 1102 16-17 0.319566779669465 1102 18-19 0.518840776985364 1102 20-21 0.09298742218516765 1102 22-23 0.029036712189721925 1102 24-25 0.3056803191623416 1102 26-27 0.08320518557374612 1102 28-29 0.027314087039080448 1102 30-31 0.071608793606174 1102 32-33 0.15932132270403088 1102 34-35 -0.03617638986028027 1102 36-37 0.09251485185419739 1102 38-39 -0.08296226126586959 1102 40-41 -0.2005834020293733 1102 42-43 0.0525070575488229 1102 44-45 -0.4555842469929061 1102 46-47 -0.7933668765427768 1102 48-49 -0.15147043710325647 1102 50-51 -0.007805986390131636 1102 52-53 0.23195865026073648 1102 54-55 0.06406065192056332 1102 56-57 0.22382467764077063 1102 58-59 0.01649334741492936 1102 60-61 -0.18025757067500336 1102 62-63 -0.26624748831866185 1102 64-65 -0.216472457788619 1102 66-67 -0.15544964151659002 1102 68-69 -0.14457140802512924 1102 70-71 -0.03432665640030308 1102 72-73 0.15097044368080503 1102 74-75 -0.035615751205192225 1102 76-77 -0.08200691594269927 1102 78-79 -0.17066709016732062 1102 80-81 0.052568216271332346 1102 82-83 -0.12619881493517227 1102 84-85 0.15189225319520006 1102 86-87 -0.005016187388790172 1102 88-89 0.08401864580938678 1102 90-91 0.10170569240867167 1102 92-93 0.11009258546607015 1102 94-95 0.30257439263997554 1102 96-97 0.16220488824490076 1102 98-99 0.09447179092981983 1102 100-101 0.06759278612021191 1103 1 0.07944444733107758 1103 2 -0.025387275079083338 1103 3 -0.18917802200734712 1103 4 0.10686802241997384 1103 5 0.15372582275441005 1103 6 0.11938195267286034 1103 7 0.1696312326554974 1103 8 0.05324639396650355 1103 9 0.35177566581462116 1103 10-11 0.15379355759984037 1103 12-13 0.31223281865774055 1103 14-15 0.3546302740707077 1103 16-17 0.2956675936343558 1103 18-19 0.47548488799840527 1103 20-21 0.2223784195373284 1103 22-23 0.22745781643606477 1103 24-25 0.36320704270847415 1103 26-27 0.08691215821275478 1103 28-29 0.06537430082745033 1103 30-31 0.24616013223518252 1103 32-33 -0.02467397002912719 1103 34-35 0.21245556071341554 1103 36-37 0.11908148910044503 1103 38-39 0.1737995314182541 1103 40-41 -0.10034803868722264 1103 42-43 -0.19260424969840528 1103 44-45 0.007532355929519952 1103 46-47 0.11005569872445875 1103 48-49 0.11006727006551387 1103 50-51 0.0227422140609832 1103 52-53 -0.26411862736993896 1103 54-55 -0.20409664924644488 1103 56-57 -0.03991956086075987 1103 58-59 -0.10102160796409976 1103 60-61 -0.047865690710303 1103 62-63 0.06819401408400694 1103 64-65 0.10936847581930209 1103 66-67 -0.30678827052512503 1103 68-69 -0.31024758729353863 1103 70-71 -0.026441984437958865 1103 72-73 -0.007821357829442377 1103 74-75 0.004435244185607701 1103 76-77 -0.4360914506097586 1103 78-79 -0.1627824182049764 1103 80-81 -0.14018455415133957 1103 82-83 -0.25422666626398893 1103 84-85 -0.45529613554658255 1103 86-87 -0.13120035920402273 1103 88-89 0.10075886352047547 1103 90-91 -0.1425818431409951 1103 92-93 -0.36338000451137376 1103 94-95 -0.49530733728058607 1103 96-97 -0.44158053884149595 1103 98-99 -0.348001485524037 1103 100-101 -0.31530414434953613 1104 1 0.3378683148002892 1104 2 0.46547980279580514 1104 3 0.43613550873077855 1104 4 0.22491956631427712 1104 5 0.07167673865549773 1104 6 0.27905994309456617 1104 7 0.4634165223336737 1104 8 0.4969897553141749 1104 9 0.4988565222706498 1104 10-11 0.7124764489775117 1104 12-13 0.6029452275800864 1104 14-15 0.6761758455072666 1104 16-17 0.6031550617222408 1104 18-19 0.8597682862430105 1104 20-21 0.599949557938082 1104 22-23 0.6510960774352625 1104 24-25 0.5241335656386141 1104 26-27 0.459369903773009 1104 28-29 0.5784298512066712 1104 30-31 0.6201925195411491 1104 32-33 0.5430356250363744 1104 34-35 0.6060675466254963 1104 36-37 0.7933524020199556 1104 38-39 0.6997671441122932 1104 40-41 0.7459762443383298 1104 42-43 0.35653389017450365 1104 44-45 0.5324321938066348 1104 46-47 0.529937181310224 1104 48-49 0.6896994188458621 1104 50-51 0.747284263517777 1104 52-53 0.7655983098120984 1104 54-55 0.6950945998451132 1104 56-57 0.605443165785168 1104 58-59 0.41145253623208333 1104 60-61 0.6451481176865386 1104 62-63 0.84499739816615 1104 64-65 0.7826647879897024 1104 66-67 0.5619760549878023 1104 68-69 0.49665661112995707 1104 70-71 0.6351143951904632 1104 72-73 0.6244615931796318 1104 74-75 0.3123960365378764 1104 76-77 0.4088575831751555 1104 78-79 0.41758394222959083 1104 80-81 0.7646642243366344 1104 82-83 0.8126406352286537 1104 84-85 0.5772998803302798 1104 86-87 0.6786108701346265 1104 88-89 0.7271233977433127 1104 90-91 0.663221037801172 1104 92-93 0.7644305912436167 1104 94-95 0.6836073711777502 1104 96-97 0.6980314843402127 1104 98-99 0.8196428876940125 1104 100-101 0.6444651566586082 1105 1 0.26139445002296924 1105 2 0.10316646395148155 1105 3 0.09686544298914868 1105 4 0.13802212395793845 1105 5 0.17669658886535444 1105 6 0.27434130518439304 1105 7 0.25084365840429257 1105 8 0.19833190623253216 1105 9 0.21078899962650866 1105 10-11 0.0863007808290277 1105 12-13 0.07131793611372217 1105 14-15 0.29999574254870964 1105 16-17 0.1752810387108923 1105 18-19 0.4759478925028304 1105 20-21 0.42341928237673443 1105 22-23 0.253213787937959 1105 24-25 0.24919308078970204 1105 26-27 0.24658949073254632 1105 28-29 -0.02955777389932024 1105 30-31 0.07476232522551385 1105 32-33 -0.008594433751468955 1105 34-35 -0.22258203597018422 1105 36-37 0.06352812693400978 1105 38-39 -0.02929434364231298 1105 40-41 -0.02816420077468962 1105 42-43 0.31159329889015197 1105 44-45 0.23621171633724458 1105 46-47 0.2615389282706033 1105 48-49 0.20331960752042733 1105 50-51 -0.0916611741270188 1105 52-53 -0.08680943726891144 1105 54-55 0.09579388069631278 1105 56-57 0.1369391868735761 1105 58-59 -0.05327830627539498 1105 60-61 -0.049540044208860934 1105 62-63 -0.034831666514669735 1105 64-65 0.12707391163575465 1105 66-67 0.08732188147674691 1105 68-69 0.09619858394634662 1105 70-71 0.14559828618637738 1105 72-73 0.049142552943493456 1105 74-75 0.11399682557928514 1105 76-77 0.11833849752655823 1105 78-79 0.24437800029367196 1105 80-81 0.30900877714812935 1105 82-83 0.41761081556577295 1105 84-85 0.45491508782617274 1105 86-87 0.2365454549122319 1105 88-89 0.16937684891250626 1105 90-91 0.26943473888078273 1105 92-93 0.12778522349142207 1105 94-95 0.04214757361796728 1105 96-97 0.3918096232100794 1105 98-99 0.12208806457197241 1105 100-101 0.4434866601656573 1106 1 -0.29279352304663675 1106 2 -0.18582593995046182 1106 3 -0.029805900967026844 1106 4 0.15938024692567865 1106 5 0.3515147817225923 1106 6 0.2298879409089878 1106 7 0.06259062603654542 1106 8 0.22303946320327128 1106 9 0.036922005549577364 1106 10-11 0.008548713152244147 1106 12-13 0.047244511663194544 1106 14-15 0.1194821626682554 1106 16-17 0.2333598195573856 1106 18-19 0.27140473776100293 1106 20-21 0.07626545801794293 1106 22-23 0.1456794166061428 1106 24-25 0.040460603760145375 1106 26-27 0.043623234677923506 1106 28-29 0.2878390446443291 1106 30-31 0.1549918946900064 1106 32-33 -0.006842136707604141 1106 34-35 0.01700874617156245 1106 36-37 0.20689773174802184 1106 38-39 0.07463930181014433 1106 40-41 0.10201005074655001 1106 42-43 0.12775220980127955 1106 44-45 -0.002776977214843157 1106 46-47 0.0834505679753974 1106 48-49 0.009585376507303067 1106 50-51 -0.1480146422922175 1106 52-53 -0.20042540931311237 1106 54-55 -0.08204824729052262 1106 56-57 0.06233754352589216 1106 58-59 0.09103069166030053 1106 60-61 -0.0664221360366426 1106 62-63 0.11368689198068438 1106 64-65 0.31754472130155165 1106 66-67 0.15615730384947568 1106 68-69 -0.0749666008290788 1106 70-71 0.06468904808985343 1106 72-73 -0.0638308539672039 1106 74-75 0.1270842414218052 1106 76-77 0.29283456455571866 1106 78-79 0.13382306687758927 1106 80-81 0.15652014431085348 1106 82-83 0.19441977977101743 1106 84-85 -0.05492408537296001 1106 86-87 -0.10243379411296161 1106 88-89 0.15978728106707507 1106 90-91 -0.0567640414260282 1106 92-93 0.11561864902132868 1106 94-95 -0.10218672215763291 1106 96-97 0.08407320646358585 1106 98-99 -0.044835515713252505 1106 100-101 0.12259536667023951 1107 1 -0.21471853158564613 1107 2 -0.28015667152201473 1107 3 -0.2091440330117038 1107 4 0.16385848194446595 1107 5 0.004578071580780829 1107 6 -0.19953241861485083 1107 7 -0.12792728057833358 1107 8 -0.13109847655561424 1107 9 0.14640473145376376 1107 10-11 0.13807280580364534 1107 12-13 -0.0423043757357604 1107 14-15 0.11562537763618508 1107 16-17 0.13462201136247387 1107 18-19 0.10290323869080709 1107 20-21 0.1782718085094146 1107 22-23 0.17845813613453743 1107 24-25 0.20007837248586924 1107 26-27 0.14443096153088675 1107 28-29 0.29579613455059217 1107 30-31 0.22102846694266276 1107 32-33 0.4343541466103318 1107 34-35 -0.0066506296586155145 1107 36-37 0.2693700590386925 1107 38-39 0.44901785182343446 1107 40-41 0.3618030272547941 1107 42-43 0.3314454886536353 1107 44-45 0.033619751396535946 1107 46-47 0.035535667091131984 1107 48-49 0.027536267726667063 1107 50-51 -0.1366577623822849 1107 52-53 0.06919058835921987 1107 54-55 0.2770344103016029 1107 56-57 0.1266046436789665 1107 58-59 0.1707692082765817 1107 60-61 0.283079438935836 1107 62-63 0.3031168657943937 1107 64-65 0.5535676311422861 1107 66-67 0.37080423170435495 1107 68-69 0.25521151654116636 1107 70-71 0.484994807808512 1107 72-73 0.48582505565846645 1107 74-75 0.3873784892933756 1107 76-77 0.5062955191534968 1107 78-79 0.46459891476940385 1107 80-81 0.19789321670898374 1107 82-83 0.3358795072818026 1107 84-85 0.46553474396074535 1107 86-87 0.2100828095126417 1107 88-89 0.25351079433099954 1107 90-91 0.26008848042750543 1107 92-93 0.2592479169437567 1107 94-95 0.24134861668283492 1107 96-97 0.30155856364710587 1107 98-99 0.01573292917536051 1107 100-101 0.16518920087434097 1108 1 0.3125836059071361 1108 2 0.051570653723509 1108 3 0.25221356375316617 1108 4 0.1326770839086464 1108 5 0.32913035349874775 1108 6 0.28308745429098536 1108 7 0.11498721971051395 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-1.8796065957376697 2316 52-53 -1.638745892033299 2316 54-55 -1.3870474250948917 2316 56-57 -1.6078272610199278 2316 58-59 -1.8566068662359712 2316 60-61 -1.403707337428898 2316 62-63 -1.131443882801669 2316 64-65 -1.5621760519277572 2316 66-67 -1.730266647622706 2316 68-69 -2.014925765112899 2316 70-71 -1.6279171293399628 2316 72-73 -1.3672050534684104 2316 74-75 -1.6069309052487597 2316 76-77 -1.3036261116545091 2316 78-79 -1.5698896031570442 2316 80-81 -1.3813099900724595 2316 82-83 -1.3837733083044057 2316 84-85 -1.9325516855666294 2316 86-87 -1.3149907768377176 2316 88-89 -1.3591072312338852 2316 90-91 -1.507033767269462 2316 92-93 -1.4753184712841616 2316 94-95 -1.547260628409063 2316 96-97 -1.6467216739983073 2316 98-99 -1.1226506221875852 2316 100-101 -1.6761083048900147 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 37.0 8 128.0 9 174.0 10 170.0 11 248.0 12 368.0 13 436.0 14 646.0 15 821.0 16 1003.0 17 1246.0 18 1489.0 19 1797.0 20 2064.0 21 2513.0 22 2920.0 23 3096.0 24 3832.0 25 4254.0 26 4914.0 27 5827.0 28 6783.0 29 8219.0 30 9837.0 31 12007.0 32 15179.0 33 19775.0 34 27768.0 35 43449.0 36 76613.0 37 139710.0 38 166704.0 39 40565.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.793990275160446 17.070151123873718 11.576912978306893 25.55894562265894 2 15.803155684298769 19.346452967687853 39.94173355134835 24.908657796665036 3 19.586981344390473 23.854719254372228 28.59874944275129 27.959549958486008 4 12.604292551278034 15.453947161203585 37.344731951773035 34.59702833574534 5 13.959126145172657 37.496331302076854 34.7817435642333 13.762798988517183 6 33.620253919747306 35.581246198744154 17.454041056048947 13.34445882545959 7 29.58643420679781 31.089601779076954 21.1570297236432 18.166934290482036 8 26.733569082599516 35.53307062866839 19.582710542101793 18.1506497466303 9 27.214125328317767 14.770612556031518 18.50129669983677 39.51396541581394 10-11 25.757314603352437 25.045493567922144 28.41168612918516 20.78550569954026 12-13 26.366641009048656 22.316410629048015 28.162619032428854 23.15432932947447 14-15 23.38790134668863 23.917146995555232 25.006546172891888 27.688405484864255 16-17 23.77824146227809 26.74654878302131 25.632326516288323 23.84288323841228 18-19 23.6740808882837 26.717091205101244 26.51581771388721 23.093010192727846 20-21 24.464499248289425 25.888449127635326 26.348572080691874 23.298479543383376 22-23 24.2475464008918 25.735087376906563 26.02320127286139 23.99416494934025 24-25 23.96662708270622 25.91139588040131 26.09936456166001 24.02261247523246 26-27 24.055608297415574 26.051400709865007 26.001286754368845 23.891704238350574 28-29 23.970100327146547 26.03345561358998 26.04842364320187 23.948020416061606 30-31 24.239276771271967 25.98474749512919 25.93347579148625 23.842499942112592 32-33 23.99259371971248 26.104574428320504 26.05801641356087 23.844815438406144 34-35 24.179652741713003 26.168167880096988 26.031140117296424 23.621039260893582 36-37 24.24208844534271 26.03171899136981 25.972839228476634 23.75335333481084 38-39 24.202146134278937 26.027253391375105 26.002857983996613 23.76774249034934 40-41 24.231668712021726 25.87525759896266 26.026757213597918 23.866316475417698 42-43 23.98159511231811 25.982928176612823 26.100108828325798 23.935367882743268 44-45 24.018989228506697 26.160204433138574 25.880152231805713 23.940654106549015 46-47 24.023632845678016 26.0315132230412 25.900670785013197 24.044183146267592 48-49 23.95937481377502 25.949912929476188 26.07985658193568 24.010855674813115 50-51 23.90934862945574 26.14625758586278 25.98829290573919 23.95610087894229 52-53 23.882269407292043 26.076187575824285 26.085952667442356 23.955590349441323 54-55 23.823707839409668 26.053430243480953 26.186099595536415 23.936762321572964 56-57 23.90078161673211 26.112174957632416 25.96307086542466 24.023972560210815 58-59 24.010988920029863 26.070873402277577 26.062761665587864 23.8553760121047 60-61 24.102265989619045 26.017641661615308 26.05002003981464 23.830072308951006 62-63 23.861937019831757 26.084678716257663 26.094791884265568 23.95859237964501 64-65 23.88630544874024 26.012012996691208 26.271930260364396 23.82975129420415 66-67 23.853413785938564 26.048614278654203 26.010617558458243 24.087354376948987 68-69 23.88167692787846 25.987646809007348 26.029949989527683 24.100726273586506 70-71 23.781658181444968 25.83607230385564 26.025448215868675 24.35682129883072 72-73 23.76831983051829 25.8413260619502 26.155297544666873 24.23505656286464 74-75 23.712513260573058 25.897226314608286 26.12198319375053 24.268277231068126 76-77 23.58449596470087 25.849865987293242 26.166540416806868 24.39909763119902 78-79 23.75014077788893 25.650725088938504 26.155703657575174 24.443430475597395 80-81 23.74461837424831 25.85174934907364 25.938153305778965 24.46547897089908 82-83 23.70772817906193 25.837738781935883 26.188353723793895 24.266179315208294 84-85 23.814518071640897 25.657649126321445 26.07965047891111 24.448182323126552 86-87 23.565254955444036 25.794790990823984 26.224792447333922 24.41516160639805 88-89 23.62210068960151 25.654566840688066 26.1783693088837 24.544963160826725 90-91 23.60715430030476 25.639306096124216 26.28244136399651 24.47109823957451 92-93 23.70806443463142 25.52021596386465 26.264227712988063 24.50749188851586 94-95 23.46844224659109 25.522360498922282 26.39389289715586 24.615304357330768 96-97 23.328792247820775 25.54617174904074 26.38194115680153 24.743094846336948 98-99 23.479153218465 25.33467401559164 26.43750931029346 24.7486634556499 100-101 23.550736330289045 25.402215817551284 26.282568455092413 24.76447939706726 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8.0 1 5.5 2 3.5 3 9.5 4 8.5 5 6.5 6 16.0 7 26.0 8 37.5 9 45.0 10 41.5 11 44.0 12 49.0 13 59.5 14 62.5 15 62.0 16 96.5 17 107.0 18 91.0 19 101.5 20 127.5 21 168.0 22 193.0 23 237.0 24 316.5 25 381.0 26 499.5 27 662.5 28 830.5 29 1137.5 30 1661.0 31 2401.5 32 3482.5 33 4986.5 34 6705.0 35 8339.0 36 10322.5 37 12450.0 38 14028.0 39 15644.5 40 17442.0 41 18793.5 42 20094.5 43 21974.0 44 23579.0 45 24734.5 46 25947.5 47 26954.0 48 28037.5 49 28623.5 50 28495.0 51 28130.5 52 27376.0 53 26509.5 54 25393.0 55 23461.5 56 21198.0 57 18958.0 58 16771.5 59 14674.0 60 12497.0 61 10277.0 62 8149.0 63 6107.0 64 4324.5 65 3071.5 66 2116.0 67 1474.0 68 1096.0 69 809.5 70 567.0 71 414.0 72 307.5 73 228.5 74 162.5 75 107.5 76 78.5 77 52.0 78 33.5 79 27.0 80 20.5 81 17.5 82 19.0 83 12.0 84 9.5 85 11.0 86 8.0 87 5.0 88 6.0 89 4.5 90 0.5 91 2.0 92 3.0 93 1.5 94 0.5 95 0.5 96 0.5 97 1.0 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47368438462378143 2 0.36667537734319955 3 0.2002904293922484 4 0.2300610960236313 5 0.2558623404374965 6 0.1989672886530758 7 0.19235158495721294 8 0.19119383681043695 9 0.19301315532679922 10-11 0.20508681457174896 12-13 0.19226888866101466 14-15 0.20111739235423123 16-17 0.08658302211960531 18-19 0.03646906662344407 20-21 1.6539259239657173E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.13330642947163682 46-47 0.2025232293896021 48-49 0.08501179249183786 50-51 0.06169143696392126 52-53 0.07095342213812927 54-55 0.0807942813857253 56-57 0.11271505171826365 58-59 0.0924544591496836 60-61 0.1362834961347751 62-63 0.23948847379023588 64-65 0.12834465169973966 66-67 0.10295688876686591 68-69 0.10725709616917678 70-71 0.08807155545117445 72-73 0.07062263695333613 74-75 0.14198954057245683 76-77 0.21848361455587126 78-79 0.13793742205874085 80-81 0.07988462212754416 82-83 0.08939469619034703 84-85 0.09849128877215847 86-87 0.07244195546969842 88-89 0.09551422210902018 90-91 0.11668447393578137 92-93 0.16580607387756316 94-95 0.13239677021345567 96-97 0.08401943693745845 98-99 0.07475745176325042 100-101 0.06210491844491269 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 604622.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.32793402402378 #Duplication Level Percentage of deduplicated Percentage of total 1 79.72283182903723 56.864648889099115 2 12.52198500184133 17.863346401223072 3 3.983653426934756 8.524373064329355 4 1.633875946873543 4.661639829681418 5 0.7949072824429403 2.8349547098653045 6 0.42209064972254456 1.8064112409340194 7 0.2544028156089531 1.2702219073096908 8 0.16471807555926402 0.9399200020844277 9 0.10593608420059923 0.6800581822161392 >10 0.3907137148469657 4.276496577836838 >50 0.003954670067968097 0.18534186402300515 >100 9.305028638799409E-4 0.09258733139765372 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.6539259239657173E-4 0.0 10-11 0.0 0.0 0.0 3.3078518479314346E-4 0.0 12-13 0.0 0.0 0.0 3.3078518479314346E-4 3.3078518479314346E-4 14-15 0.0 0.0 0.0 4.961777771897152E-4 3.3078518479314346E-4 16-17 0.0 0.0 0.0 0.0010750518505777164 3.3078518479314346E-4 18-19 0.0 0.0 0.0 0.0015712296277674315 3.3078518479314346E-4 20-21 0.0 0.0 0.0 0.002067407404957147 3.3078518479314346E-4 22-23 0.0 0.0 0.0 0.0029770666631382912 4.961777771897152E-4 24-25 0.0 0.0 0.0 0.003969422217517722 4.961777771897152E-4 26-27 0.0 0.0 0.0 0.004961777771897153 4.961777771897152E-4 28-29 0.0 0.0 0.0 0.006119525918673154 4.961777771897152E-4 30-31 0.0 0.0 0.0 0.011577481467760022 4.961777771897152E-4 32-33 0.0 0.0 0.0 0.0228241777507269 6.615703695862869E-4 34-35 0.0 0.0 0.0 0.03845377773220293 6.615703695862869E-4 36-37 0.0 0.0 0.0 0.06160874066772297 6.615703695862869E-4 38-39 0.0 0.0 0.0 0.10915911098173735 6.615703695862869E-4 40-41 0.0 0.0 0.0 0.17498536275557292 6.615703695862869E-4 42-43 0.0 0.0 0.0 0.2335343404639593 6.615703695862869E-4 44-45 0.0 0.0 0.0 0.29216601446854396 6.615703695862869E-4 46-47 0.0 0.0 0.0 0.37370456252005385 6.615703695862869E-4 48-49 0.0 0.0 0.0 0.46045297723205575 6.615703695862869E-4 50-51 0.0 0.0 0.0 0.5693639993251982 6.615703695862869E-4 52-53 0.0 0.0 0.0 0.6748017769780127 6.615703695862869E-4 54-55 0.0 0.0 0.0 0.7739546361197575 6.615703695862869E-4 56-57 0.0 0.0 0.0 0.9018858063385057 7.442666657845728E-4 58-59 0.0 0.0 0.0 1.0459427543159197 8.269629619828587E-4 60-61 0.0 0.0 0.0 1.2062081763481978 8.269629619828587E-4 62-63 0.0 0.0 0.0 1.3768933317014598 8.269629619828587E-4 64-65 0.0 0.0 0.0 1.5301295685568834 8.269629619828587E-4 66-67 0.0 0.0 0.0 1.7115652424159227 8.269629619828587E-4 68-69 0.0 0.0 0.0 1.9298834643793974 8.269629619828587E-4 70-71 0.0 0.0 0.0 2.1854150196321007 8.269629619828587E-4 72-73 0.0 0.0 0.0 2.442765893401166 9.096592581811446E-4 74-75 0.0 0.0 0.0 2.685893004224127 9.923555543794305E-4 76-77 0.0 0.0 0.0 2.9435746631779858 9.923555543794305E-4 78-79 0.0 0.0 0.0 3.25211454429379 9.923555543794305E-4 80-81 0.0 0.0 0.0 3.6206092401533523 0.0010750518505777164 82-83 0.0 0.0 0.0 4.018543817459504 0.0016539259239657174 84-85 0.0 0.0 0.0 4.393488824422532 0.0016539259239657174 86-87 0.0 0.0 0.0 4.767772261015974 0.0017366222201640033 88-89 0.0 0.0 0.0 5.207551164198458 0.0018193185163622892 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 785 0.0 37.613846 1 GGTATCA 605 0.0 33.84845 1 TACCGTA 130 8.223651E-9 32.904846 7 GTACATG 2095 0.0 31.370453 1 TACATGG 2070 0.0 30.336155 2 TCAACGC 910 0.0 30.300888 4 CAACGCA 940 0.0 29.33384 5 TATCAAC 1005 0.0 28.874916 2 ACATGGG 2165 0.0 28.54422 3 ATCAACG 975 0.0 28.278482 3 GGACCGA 120 5.350461E-6 27.72538 6 AACGCAG 995 0.0 27.705477 6 ACCGTAT 155 4.5525667E-8 27.597616 8 TACACCG 110 9.413861E-5 25.93148 5 CATGGGG 1435 0.0 25.841127 4 CCGTATG 150 1.0530512E-6 25.34892 9 GTAGGAC 250 1.4551915E-11 24.719296 3 TGTACCG 195 1.5523256E-8 24.380026 5 TAACGAT 50 0.0016374731 23.79025 46-47 GTACCGT 180 1.936296E-7 23.764612 6 >>END_MODULE