FastQCFastQC Report
Fri 10 Feb 2017
SRR2031650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604622
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7450.037.010381
GGTATCA4850.036.2670141
GAACGCG352.0529279E-433.92988630-31
TCAACGC7850.033.2884254
ATCAACG8350.031.2976973
CAACGCA8450.030.914525
GTACATG16600.030.642731
TATCAAC9150.029.0805822
AACGCAG9300.028.599716
TATACTA2002.3646862E-1128.497575
ACATGGG17550.027.3451583
TACATGG17650.027.1902282
GTATAAG2500.026.6219481
ACGCAGA10600.024.6441237
GTATTAC1751.4719262E-724.4487291
GTATAGA2351.7462298E-1024.2753331
CGCAGAG10450.024.0888548
CATGGGG12150.023.0715084
CCTTACG1050.002190841822.6264742
GTATAGT1954.1812564E-721.9411681
Produced by FastQC (version 0.11.5)