##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031650_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 604622 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47346441247589 34.0 31.0 34.0 31.0 34.0 2 32.692206039475906 34.0 31.0 34.0 31.0 34.0 3 32.81275077651822 34.0 31.0 34.0 31.0 34.0 4 36.25855327791579 37.0 37.0 37.0 35.0 37.0 5 35.91237169669645 37.0 35.0 37.0 35.0 37.0 6 35.96191008597107 37.0 36.0 37.0 35.0 37.0 7 35.934487994151716 37.0 36.0 37.0 35.0 37.0 8 36.053468117269965 37.0 36.0 37.0 35.0 37.0 9 37.98001561306072 39.0 39.0 39.0 35.0 39.0 10-11 37.944513266139836 39.0 38.5 39.0 35.0 39.0 12-13 37.95838887767894 39.0 38.0 39.0 35.0 39.0 14-15 39.44545434999057 41.0 39.0 41.0 36.0 41.0 16-17 39.40697741729544 41.0 39.0 41.0 36.0 41.0 18-19 39.38789524694768 41.0 39.0 41.0 36.0 41.0 20-21 39.3991386353788 41.0 39.0 41.0 36.0 41.0 22-23 39.33975111722696 41.0 39.0 41.0 36.0 41.0 24-25 39.27388765211984 41.0 39.0 41.0 36.0 41.0 26-27 39.16951582972502 41.0 39.0 41.0 36.0 41.0 28-29 39.09446728699915 40.5 39.0 41.0 36.0 41.0 30-31 39.01373833568742 40.0 39.0 41.0 35.0 41.0 32-33 38.89825626589836 40.0 38.0 41.0 35.0 41.0 34-35 38.807060444376816 40.0 38.0 41.0 35.0 41.0 36-37 38.6849866528178 40.0 38.0 41.0 35.0 41.0 38-39 38.58207524701384 40.0 38.0 41.0 34.5 41.0 40-41 38.478940561210145 40.0 38.0 41.0 34.0 41.0 42-43 38.337694460340515 40.0 38.0 41.0 34.0 41.0 44-45 38.30774847756119 40.0 38.0 41.0 34.0 41.0 46-47 38.36994105408007 40.0 38.0 41.0 33.5 41.0 48-49 38.53061913063038 40.0 38.0 41.0 34.0 41.0 50-51 38.457915027901734 40.0 38.0 41.0 34.0 41.0 52-53 38.344982484924465 40.0 38.0 41.0 34.0 41.0 54-55 38.22166990284839 40.0 37.0 41.0 34.0 41.0 56-57 38.08314781797553 40.0 37.0 41.0 34.0 41.0 58-59 37.858240355130974 40.0 36.5 41.0 33.0 41.0 60-61 37.540804833433114 39.0 36.0 41.0 33.0 41.0 62-63 37.32114941236012 39.0 35.5 41.0 33.0 41.0 64-65 36.98724905808919 39.0 35.0 41.0 32.0 41.0 66-67 36.67185861579632 37.5 35.0 40.0 32.0 41.0 68-69 36.34800255366163 37.0 35.0 40.0 32.0 41.0 70-71 35.98899312297601 37.0 35.0 39.0 31.5 41.0 72-73 35.592580984482865 36.0 35.0 39.0 31.0 41.0 74-75 35.22339412062412 35.5 35.0 38.5 31.0 40.0 76-77 34.61284240401441 35.0 34.0 37.0 30.0 39.0 78-79 34.561428462742015 35.0 34.0 37.0 31.0 39.0 80-81 34.30955869948497 35.0 34.0 36.0 30.5 38.5 82-83 34.05471683134256 35.0 34.0 36.0 30.0 37.0 84-85 33.829960702720044 35.0 34.0 36.0 30.0 37.0 86-87 33.62448852340802 35.0 34.0 35.0 30.0 36.5 88-89 33.437915755629135 35.0 34.0 35.0 29.5 36.0 90-91 33.31712789147599 35.0 34.0 35.0 29.0 36.0 92-93 33.220755281812444 35.0 34.0 35.0 29.0 36.0 94-95 33.11584097171456 35.0 34.0 35.0 29.0 35.0 96-97 33.042883818319545 35.0 34.0 35.0 29.0 35.0 98-99 32.97529282758484 35.0 34.0 35.0 29.0 35.0 100-101 32.54172524320981 34.5 32.5 35.0 28.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.23384833925668147 1101 2 0.058796729965926886 1101 3 0.05027596272142176 1101 4 0.05418446648310038 1101 5 0.1924362912565485 1101 6 0.2157693575706574 1101 7 0.23678253169389762 1101 8 0.033624572180315226 1101 9 0.08829221070218551 1101 10-11 0.02670636165752427 1101 12-13 0.05998613925358143 1101 14-15 0.0781254869891086 1101 16-17 0.28243347232547933 1101 18-19 0.17582150169267408 1101 20-21 0.0756079267261427 1101 22-23 0.16906475445488667 1101 24-25 -0.05873690308920487 1101 26-27 0.1825618187736069 1101 28-29 0.04618768439829779 1101 30-31 0.05931541976592314 1101 32-33 0.019578820725200785 1101 34-35 0.17961768835429126 1101 36-37 0.12386653782721879 1101 38-39 0.2586943984309542 1101 40-41 0.08046832897952072 1101 42-43 -0.04876279734607891 1101 44-45 -0.07383034861341997 1101 46-47 0.07730460838283904 1101 48-49 0.1405416351705142 1101 50-51 0.42331436130307054 1101 52-53 0.0636724819111194 1101 54-55 0.22227689249949378 1101 56-57 0.2866352072958094 1101 58-59 0.2017273752443529 1101 60-61 0.1226839594458724 1101 62-63 0.12029014174757435 1101 64-65 0.23448996966816793 1101 66-67 0.08136927004186134 1101 68-69 0.2742357046354442 1101 70-71 0.19608150382116207 1101 72-73 0.1365773112055635 1101 74-75 0.09238371621906794 1101 76-77 0.013308368715556185 1101 78-79 0.18330679806130945 1101 80-81 0.07778347368127214 1101 82-83 0.050501504299596434 1101 84-85 -0.015006966307232972 1101 86-87 -0.15745738635715156 1101 88-89 -0.11714049431586204 1101 90-91 0.1379255857639734 1101 92-93 -0.2797169422084451 1101 94-95 0.0666942894665965 1101 96-97 0.010615754844231162 1101 98-99 -0.09016798856623609 1101 100-101 -0.11605091203394124 1102 1 0.1787311294224878 1102 2 0.14947711875952052 1102 3 0.2357533783875212 1102 4 0.1471899934508869 1102 5 -0.019608686826167343 1102 6 0.02716634632613335 1102 7 0.2321703723647559 1102 8 0.07208464460264707 1102 9 0.20778906604809322 1102 10-11 0.16082109388545973 1102 12-13 0.08520054748685624 1102 14-15 -0.07441882404576461 1102 16-17 -0.09747885853025196 1102 18-19 0.16979901265131758 1102 20-21 0.08473695283630178 1102 22-23 0.08343487071180533 1102 24-25 0.14532255946082984 1102 26-27 0.23861289557940069 1102 28-29 -0.10469853629923875 1102 30-31 -0.0569605474534427 1102 32-33 0.007381374565490262 1102 34-35 0.011064229234797551 1102 36-37 0.12819281951198747 1102 38-39 -0.059563653789361126 1102 40-41 0.12106295447981097 1102 42-43 0.11252936960645599 1102 44-45 0.1502977245712529 1102 46-47 0.22855294076325094 1102 48-49 -4.913360511338283E-4 1102 50-51 0.24591775371778368 1102 52-53 0.08261664047791584 1102 54-55 -0.22022358396324648 1102 56-57 -0.3319468395881202 1102 58-59 -0.2253166499128767 1102 60-61 0.1442581923408568 1102 62-63 -0.07016154493052795 1102 64-65 -0.024686702716046227 1102 66-67 0.24195627213829596 1102 68-69 0.03895840332040734 1102 70-71 0.10673521070128089 1102 72-73 0.042294864092930595 1102 74-75 0.2490446653328462 1102 76-77 0.1565902440729019 1102 78-79 -0.15736406147748028 1102 80-81 -0.022006882712858555 1102 82-83 -0.3633921492167005 1102 84-85 -0.1599850490211665 1102 86-87 -0.11979777133903724 1102 88-89 -0.05213191798652872 1102 90-91 0.06860978625949343 1102 92-93 0.011287441248775565 1102 94-95 0.16396892259028562 1102 96-97 0.22205086061895685 1102 98-99 0.3237066064404317 1102 100-101 0.07927975310804669 1103 1 0.13279605047770104 1103 2 0.12521508090524947 1103 3 0.15673013625747956 1103 4 0.09964659833467948 1103 5 -0.03696673331042888 1103 6 6.0334754103053E-5 1103 7 0.2757518178879508 1103 8 0.12655164470071867 1103 9 0.15838237780645414 1103 10-11 0.009737441830928617 1103 12-13 0.048042559843430865 1103 14-15 0.20494765629158707 1103 16-17 0.16968795523850133 1103 18-19 0.15604987074683407 1103 20-21 0.21743284378462135 1103 22-23 0.09067229348320183 1103 24-25 0.04227264281867349 1103 26-27 0.0315716089264626 1103 28-29 0.17874757530221075 1103 30-31 0.16933149922695634 1103 32-33 0.27497968779487536 1103 34-35 0.13997469309671828 1103 36-37 0.09683065416923853 1103 38-39 0.19489353950277888 1103 40-41 0.18042355073360739 1103 42-43 0.13871354142170134 1103 44-45 0.12097537486055643 1103 46-47 0.1029767909152568 1103 48-49 -0.19547025141839924 1103 50-51 -0.11453139799350254 1103 52-53 -0.07167383711352926 1103 54-55 0.08717074770292754 1103 56-57 -0.01579503023243234 1103 58-59 0.24253664027050803 1103 60-61 0.17314904259093566 1103 62-63 -0.0020826982109056758 1103 64-65 0.14202899798023338 1103 66-67 0.1393672753745392 1103 68-69 0.32908294975563734 1103 70-71 0.300115616703053 1103 72-73 0.17752091096946288 1103 74-75 0.219330043385213 1103 76-77 0.0706041697373081 1103 78-79 0.2719779018450694 1103 80-81 0.28241598675855784 1103 82-83 0.27862020735876314 1103 84-85 -0.022238653022348842 1103 86-87 -0.2424220726041213 1103 88-89 -0.025575087546201303 1103 90-91 0.03384465926136215 1103 92-93 -0.03863900770680573 1103 94-95 -0.007336363278106717 1103 96-97 0.2002699446632903 1103 98-99 0.08739200410641246 1103 100-101 0.021831484009290136 1104 1 0.20991436290898235 1104 2 0.24018069967423372 1104 3 0.2564711123490184 1104 4 0.16056753082074948 1104 5 0.037356357087048764 1104 6 0.15394662512176893 1104 7 0.14076495543150713 1104 8 0.14917803532367913 1104 9 0.20437682463201412 1104 10-11 0.2161944047290163 1104 12-13 0.31528255760725443 1104 14-15 0.3906212116276677 1104 16-17 0.2960301276850714 1104 18-19 0.43140160285275186 1104 20-21 0.4490169892852478 1104 22-23 0.31461616542476634 1104 24-25 0.3011921963285431 1104 26-27 0.3178563861472625 1104 28-29 0.26539200442469735 1104 30-31 0.23968537433643178 1104 32-33 0.12774043542358982 1104 34-35 0.3992706698404689 1104 36-37 0.3214807855446722 1104 38-39 0.18632711570985094 1104 40-41 0.08304360551996126 1104 42-43 0.19051459615214839 1104 44-45 0.23353896616182368 1104 46-47 0.32332600732134864 1104 48-49 0.28772673719573305 1104 50-51 0.3914634215283286 1104 52-53 0.5433700478724006 1104 54-55 0.3890864065367623 1104 56-57 0.370478566100239 1104 58-59 0.46542764524600244 1104 60-61 0.385414476875269 1104 62-63 0.4620434222072589 1104 64-65 0.5363807859905947 1104 66-67 0.42884046919523655 1104 68-69 0.25074033007354046 1104 70-71 0.3777873830970293 1104 72-73 0.36664004333254496 1104 74-75 0.088979522542175 1104 76-77 0.10156591246507674 1104 78-79 0.313738519030089 1104 80-81 0.4058455378458987 1104 82-83 0.4683300633116474 1104 84-85 0.4229731316337464 1104 86-87 0.3514172580333792 1104 88-89 0.3090577134526171 1104 90-91 0.2990795510862654 1104 92-93 0.4646928969574944 1104 94-95 0.34997873278435776 1104 96-97 0.3680540789830218 1104 98-99 0.3965230627501697 1104 100-101 0.5526739639302818 1105 1 0.08740007395095262 1105 2 0.05106975491777632 1105 3 0.008575873206318363 1105 4 0.11481448233230651 1105 5 -0.06075583296406961 1105 6 -0.042902176237767264 1105 7 0.12832974800657126 1105 8 0.10575592300590131 1105 9 0.12235057765212076 1105 10-11 0.19694200727069955 1105 12-13 0.2104063952435382 1105 14-15 0.12735492079637112 1105 16-17 0.11714053316862305 1105 18-19 0.10549982857394724 1105 20-21 0.16168387118802485 1105 22-23 0.18726615957081805 1105 24-25 0.1871661338367474 1105 26-27 -0.018023710779942803 1105 28-29 0.07791129584848733 1105 30-31 0.02269366565733577 1105 32-33 0.18209524151595957 1105 34-35 0.1388728141598321 1105 36-37 0.18281669225046215 1105 38-39 0.20597155037800263 1105 40-41 0.06609553537190749 1105 42-43 0.13215072176016207 1105 44-45 0.29774259844865725 1105 46-47 0.29732382894264475 1105 48-49 0.19497534555232932 1105 50-51 0.28047963874177384 1105 52-53 0.3729917408345287 1105 54-55 0.18598452298423496 1105 56-57 0.1426960775686581 1105 58-59 0.2771273837696029 1105 60-61 0.36888150494938543 1105 62-63 0.4129057266764846 1105 64-65 0.2353405569415301 1105 66-67 0.2152156683182085 1105 68-69 0.20485955905520825 1105 70-71 0.18956162642973595 1105 72-73 0.23014191127454353 1105 74-75 0.18953085812957227 1105 76-77 0.06703293344349959 1105 78-79 0.28358890627770705 1105 80-81 0.12806500063074822 1105 82-83 0.16149442446839402 1105 84-85 0.34123603427016036 1105 86-87 0.2773399646156989 1105 88-89 0.2507452828574017 1105 90-91 0.21957227622382902 1105 92-93 0.2294244490345747 1105 94-95 0.049715482458843496 1105 96-97 0.15707952060140684 1105 98-99 -0.013196621775172446 1105 100-101 0.13205706411086737 1106 1 0.05247218965990186 1106 2 -0.015416341389411059 1106 3 0.14543044098429192 1106 4 0.04958990497488713 1106 5 0.05969898846690569 1106 6 -0.12044848815001075 1106 7 -0.04962200395316785 1106 8 -0.1191857233100535 1106 9 0.15892450805944947 1106 10-11 0.16822727245952507 1106 12-13 0.12290791311392724 1106 14-15 0.10558191325424104 1106 16-17 0.034256377037102936 1106 18-19 -0.04099321620660845 1106 20-21 0.012787531616808678 1106 22-23 0.14260872605353825 1106 24-25 -0.030840531300803775 1106 26-27 0.052737720470652505 1106 28-29 0.1920315381680595 1106 30-31 0.01896476405580927 1106 32-33 -0.03462629335458445 1106 34-35 -0.044308844568938355 1106 36-37 0.007843339308486463 1106 38-39 0.12500834355635249 1106 40-41 -0.12485424672936318 1106 42-43 -0.024724556516076746 1106 44-45 -0.36570168558873917 1106 46-47 -0.22200372074231467 1106 48-49 -0.136891837415142 1106 50-51 -0.09732673496466049 1106 52-53 0.04905290089228487 1106 54-55 0.033136660820353825 1106 56-57 -0.18939541291261008 1106 58-59 -0.10017471966146019 1106 60-61 -0.12658343561755458 1106 62-63 -0.2594670083235826 1106 64-65 -0.03631945358237232 1106 66-67 -0.09514835073275663 1106 68-69 -0.149458613563759 1106 70-71 -0.011347071979535883 1106 72-73 0.03184732298845461 1106 74-75 -0.1137345870449451 1106 76-77 -0.041675257659761655 1106 78-79 -0.18483770300221636 1106 80-81 -0.03476032959039088 1106 82-83 0.06076179902483858 1106 84-85 0.09227661875144832 1106 86-87 -0.19744652161553233 1106 88-89 0.026232013297104118 1106 90-91 0.06629097773630832 1106 92-93 -0.08873467775145372 1106 94-95 0.0636242348368441 1106 96-97 -0.24355225078707576 1106 98-99 -0.09132141095984281 1106 100-101 -0.1275679207104332 1107 1 0.23247130413319894 1107 2 0.27299654175901367 1107 3 0.17343522282846635 1107 4 0.06263561080292135 1107 5 0.3104580113514501 1107 6 0.004923116803880134 1107 7 0.14624701399772277 1107 8 0.16377800804644238 1107 9 0.028894400151692423 1107 10-11 0.06721081431330589 1107 12-13 -0.09367136050116898 1107 14-15 -0.011797813244513122 1107 16-17 -0.008791289600047492 1107 18-19 0.045827932701037355 1107 20-21 -0.02906309267951457 1107 22-23 -0.011050713028545545 1107 24-25 0.06527074145407141 1107 26-27 0.13572264121601307 1107 28-29 -0.015709862735171498 1107 30-31 0.06897646565862203 1107 32-33 0.14756133710247354 1107 34-35 0.04112392497906825 1107 36-37 0.1510330064769576 1107 38-39 0.17880092790277047 1107 40-41 0.0716520341850071 1107 42-43 -0.11279493058785306 1107 44-45 0.025663164061477062 1107 46-47 0.1722201151063345 1107 48-49 0.09257975615340541 1107 50-51 -0.14496332745792273 1107 52-53 0.009246894491191426 1107 54-55 0.062468151414798 1107 56-57 -0.08037916982288351 1107 58-59 0.04924526035093635 1107 60-61 0.15916812354195287 1107 62-63 -0.2440809819348857 1107 64-65 -0.06728600597393353 1107 66-67 0.13404177184944643 1107 68-69 0.05073999742435831 1107 70-71 0.09212988535072242 1107 72-73 0.2237156578187509 1107 74-75 0.16023630442742842 1107 76-77 0.0911490032417106 1107 78-79 0.20321749061186978 1107 80-81 0.15041753477221675 1107 82-83 0.14546242774878948 1107 84-85 0.2773659304441978 1107 86-87 -0.11238219442606123 1107 88-89 -0.15026927071660623 1107 90-91 0.20040083364845884 1107 92-93 0.37633129398759735 1107 94-95 0.37452158964208593 1107 96-97 0.14416454512432608 1107 98-99 0.06473211280389535 1107 100-101 0.18828838360714428 1108 1 -0.024097153405797656 1108 2 0.04472964401205104 1108 3 0.1425715602057025 1108 4 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2316 68-69 -0.9979690691514946 2316 70-71 -1.1476054423878068 2316 72-73 -1.196316531464653 2316 74-75 -0.9707376383042714 2316 76-77 -1.0536713202562566 2316 78-79 -0.9290926110533348 2316 80-81 -1.1860591702945626 2316 82-83 -1.6416490612658592 2316 84-85 -1.4981843132529704 2316 86-87 -1.1179242172752453 2316 88-89 -0.8788066755749284 2316 90-91 -0.6344458009012186 2316 92-93 -0.596001973550031 2316 94-95 -0.6947016378324591 2316 96-97 -0.42175515953249487 2316 98-99 -0.51267725722753 2316 100-101 -0.9649067932061612 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 5.0 14 29.0 15 38.0 16 114.0 17 200.0 18 286.0 19 504.0 20 675.0 21 839.0 22 1174.0 23 1404.0 24 1794.0 25 2264.0 26 2825.0 27 3789.0 28 4926.0 29 6169.0 30 7809.0 31 9898.0 32 12863.0 33 17179.0 34 24733.0 35 38436.0 36 72041.0 37 150093.0 38 196690.0 39 47820.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.30789265538593 17.50025648068783 11.98161943561011 25.21023142831613 2 16.0845626239421 19.701066956436886 39.65204418665039 24.562326232970623 3 19.54067093269999 24.071443102373223 28.876436643781805 27.511449321144983 4 12.671797645572092 15.587126569108595 37.39345609969515 34.34761968562416 5 13.537383687659396 37.6974374071734 35.21721009159442 13.547968813572778 6 33.43477412333657 36.093790831296246 17.385407742358037 13.086027303009153 7 29.450466572503153 31.11762390386059 21.356980063576913 18.074929460059344 8 26.454379761239256 35.85297921676684 19.783600332108325 17.90904068988558 9 26.94857282732021 14.913284663806477 18.68258184452435 39.455560664348965 10-11 25.498658666075663 25.17126402942665 28.740766958529463 20.589310345968226 12-13 26.15832702084939 22.397464862343746 28.39443487005104 23.049773246755827 14-15 23.25064255022146 23.945126045694664 25.21385262196877 27.590378782115106 16-17 23.636999646059852 27.015804916129415 25.77552586574752 23.571669572063207 18-19 23.480455557356496 26.804681271935195 26.742907138675072 22.971956032033237 20-21 24.229270519431974 25.99434026548819 26.61944156845103 23.156947646628804 22-23 24.010185111675664 25.840048428072404 26.21475402244099 23.935012437810947 24-25 23.706299142274016 26.081419465384986 26.382847465027737 23.82943392731326 26-27 23.786349156993957 26.17767795415979 26.249375642963702 23.78659724588255 28-29 23.760660572980726 26.105056116114746 26.29496340397234 23.839319906932193 30-31 24.03307674429953 26.11054639651897 26.206157309727253 23.65021954945425 32-33 23.837041986563506 26.202652235611673 26.29543747994615 23.664868297878673 34-35 24.062141304815242 26.192894072660273 26.299737687348458 23.445226935176027 36-37 24.0882733344139 26.031140117296424 26.23812894668074 23.64245760160894 38-39 23.962657660488702 25.992355554379433 26.293535465133587 23.751451319998278 40-41 24.024679882637418 25.945632147027396 26.25971267998849 23.769975290346697 42-43 23.802805719937414 25.930746813711707 26.44222340569811 23.82422406065277 44-45 23.955297690127058 26.0759615098359 26.201742576353492 23.766998223683558 46-47 23.90104891982098 25.976395169213163 26.10077039869538 24.021785512270476 48-49 23.75064629944449 25.9586120176538 26.27429568871737 24.016445994184338 50-51 23.831700724831858 26.107179838360178 26.16581810723277 23.89530132957519 52-53 23.698691083023775 26.04007131728584 26.377637598367244 23.883600001323142 54-55 23.6714796070259 25.943981343653867 26.440822334689557 23.94371671463068 56-57 23.793212949578415 26.093079643148943 26.149809302340966 23.963898104931676 58-59 23.83786894952549 25.989130398827697 26.29394894661458 23.879051705032232 60-61 23.905266430927092 26.06661682836549 26.196780798581592 23.831335942125826 62-63 23.707622283013187 26.073811406134745 26.31644233918051 23.902123971671557 64-65 23.644690401606294 26.024937895081557 26.341912798409584 23.98845890490257 66-67 23.671070520093547 26.069180413547638 26.155267257890053 24.104481808468762 68-69 23.66172583862314 25.982349302539436 26.22382248743843 24.13210237139899 70-71 23.683723053411885 25.781728087962396 26.212245005970676 24.322303852655047 72-73 23.60466539424632 25.975402813658782 26.180241539341935 24.23969025275296 74-75 23.51312059435482 25.958946250715325 26.254337420735602 24.273595734194256 76-77 23.548762698016283 25.90560713966743 26.19570574673101 24.349924415585274 78-79 23.59597763884754 25.773461016837025 26.08820416129139 24.54235718302405 80-81 23.67755936509617 25.963421573232942 25.939189233894176 24.419829827776713 82-83 23.611075504200745 25.926590588252807 26.185524040453494 24.276809867092958 84-85 23.69124841636593 25.854087347135895 26.116399998676858 24.338264237821317 86-87 23.466066401817994 25.874761421185465 26.20811019116076 24.45106198583578 88-89 23.607725157205657 25.73211031024342 26.096387494996875 24.56377703755404 90-91 23.51560148324077 25.79884622127544 26.129714102364783 24.555838193119005 92-93 23.5141129499092 25.721359791737648 26.165935080099633 24.59859217825352 94-95 23.385106727839876 25.777924058337277 26.187518813407383 24.649450400415464 96-97 23.34384127603693 25.666366754765786 26.288573687361687 24.701218281835594 98-99 23.309687705707034 25.663141599214057 26.17478358379285 24.85238711128606 100-101 23.432491705561493 25.7187135102593 26.052393065419388 24.79640171875982 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 2.0 2 3.5 3 7.0 4 6.5 5 10.0 6 13.0 7 19.0 8 32.0 9 41.0 10 44.0 11 44.0 12 47.0 13 53.5 14 51.5 15 49.5 16 61.5 17 70.5 18 73.0 19 88.0 20 106.0 21 133.5 22 163.5 23 200.5 24 263.5 25 321.5 26 426.5 27 573.5 28 751.0 29 1053.5 30 1611.5 31 2502.5 32 3737.5 33 5291.0 34 6942.0 35 8678.5 36 10597.5 37 12608.0 38 14289.0 39 15745.5 40 17365.5 41 18909.5 42 20307.5 43 21954.5 44 23584.5 45 24724.5 46 25907.0 47 27236.0 48 28436.0 49 29081.0 50 28869.5 51 28706.5 52 28117.5 53 26914.5 54 25966.0 55 24269.0 56 21773.5 57 19257.0 58 17024.0 59 14582.5 60 11853.5 61 9493.0 62 7358.0 63 5343.5 64 3570.5 65 2371.0 66 1609.0 67 1035.5 68 705.5 69 487.0 70 320.5 71 237.0 72 179.0 73 130.5 74 89.0 75 57.5 76 35.5 77 17.0 78 11.5 79 8.5 80 4.5 81 1.5 82 1.5 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04763306661021266 2 8.269629619828587E-4 3 1.6539259239657173E-4 4 0.009758162951397732 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0023154962935520045 24-25 0.0 26-27 0.0 28-29 0.01926823701420061 30-31 0.014637244427096598 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0363036740310475 50-51 0.011246696282966878 52-53 0.0 54-55 3.3078518479314346E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.3078518479314346E-4 80-81 0.00959277035900116 82-83 0.004382903698509151 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 604622.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.07113458573416 #Duplication Level Percentage of deduplicated Percentage of total 1 78.56825078011465 55.05366474579125 2 13.353405058467382 18.713764860593823 3 4.060087148989852 8.534847391400326 4 1.7073918503439434 4.785555365441445 5 0.8465650964577534 2.965988840473813 6 0.4658321820632407 1.9584833714231729 7 0.2703938530039302 1.3262762845495528 8 0.17841101796938708 1.0001169961368606 9 0.1159758685628435 0.731390462528798 >10 0.4282187843277058 4.619541258248805 >50 0.004041840000341138 0.18059180987801152 >100 0.0014265196989895442 0.1297786135342056 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.6539259239657173E-4 2 0.0 0.0 0.0 0.0 1.6539259239657173E-4 3 0.0 0.0 0.0 0.0 1.6539259239657173E-4 4 0.0 0.0 0.0 0.0 1.6539259239657173E-4 5 0.0 0.0 0.0 0.0 1.6539259239657173E-4 6 0.0 0.0 0.0 0.0 1.6539259239657173E-4 7 0.0 0.0 0.0 0.0 1.6539259239657173E-4 8 0.0 0.0 0.0 0.0 1.6539259239657173E-4 9 0.0 0.0 0.0 0.0 1.6539259239657173E-4 10-11 0.0 0.0 0.0 0.0 1.6539259239657173E-4 12-13 0.0 0.0 0.0 0.0 1.6539259239657173E-4 14-15 0.0 0.0 0.0 1.6539259239657173E-4 1.6539259239657173E-4 16-17 0.0 0.0 0.0 7.442666657845728E-4 1.6539259239657173E-4 18-19 0.0 0.0 0.0 0.0012404444429742882 1.6539259239657173E-4 20-21 0.0 0.0 0.0 0.0017366222201640033 1.6539259239657173E-4 22-23 0.0 0.0 0.0 0.0026462814783451477 1.6539259239657173E-4 24-25 0.0 0.0 0.0 0.0036386370327245784 1.6539259239657173E-4 26-27 0.0 0.0 0.0 0.004630992587104009 1.6539259239657173E-4 28-29 0.0 0.0 0.0 0.005788740733880011 1.6539259239657173E-4 30-31 0.0 0.0 0.0 0.011163999986768593 2.4808888859485757E-4 32-33 0.0 0.0 0.0 0.02183182219634747 4.961777771897152E-4 34-35 0.0 0.0 0.0 0.03812299254740979 4.961777771897152E-4 36-37 0.0 0.0 0.0 0.06069908140954183 4.961777771897152E-4 38-39 0.0 0.0 0.0 0.10791866653876306 6.615703695862869E-4 40-41 0.0 0.0 0.0 0.17424109608978833 6.615703695862869E-4 42-43 0.0 0.0 0.0 0.23204580713239015 6.615703695862869E-4 44-45 0.0 0.0 0.0 0.2903466959521817 7.442666657845728E-4 46-47 0.0 0.0 0.0 0.3714717625227001 8.269629619828587E-4 48-49 0.0 0.0 0.0 0.45879905130809 8.269629619828587E-4 50-51 0.0 0.0 0.0 0.5686197326594137 8.269629619828587E-4 52-53 0.0 0.0 0.0 0.675463347347599 8.269629619828587E-4 54-55 0.0 0.0 0.0 0.7747815990817404 8.269629619828587E-4 56-57 0.0 0.0 0.0 0.9029608581890834 8.269629619828587E-4 58-59 0.0 0.0 0.0 1.0476793765360837 8.269629619828587E-4 60-61 0.0 0.0 0.0 1.2092679393075343 9.096592581811446E-4 62-63 0.0 0.0 0.0 1.3806146650303828 9.923555543794305E-4 64-65 0.0 0.0 0.0 1.5353394352173755 9.923555543794305E-4 66-67 0.0 0.0 0.0 1.7185944275927771 9.923555543794305E-4 68-69 0.0 0.0 0.0 1.93988971621939 9.923555543794305E-4 70-71 0.0 0.0 0.0 2.1986464270238266 9.923555543794305E-4 72-73 0.0 0.0 0.0 2.457485834124461 9.923555543794305E-4 74-75 0.0 0.0 0.0 2.7025149597599825 9.923555543794305E-4 76-77 0.0 0.0 0.0 2.964744915004747 9.923555543794305E-4 78-79 0.0 0.0 0.0 3.2753522035255083 0.0011577481467760023 80-81 0.0 0.0 0.0 3.6462450919748206 0.0011577481467760023 82-83 0.0 0.0 0.0 4.047239432240309 0.0011577481467760023 84-85 0.0 0.0 0.0 4.424334542904493 0.0011577481467760023 86-87 0.0 0.0 0.0 4.8016777424572705 0.0012404444429742882 88-89 0.0 0.0 0.0 5.24550876415347 0.0014058370353708597 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 745 0.0 37.01038 1 GGTATCA 485 0.0 36.267014 1 GAACGCG 35 2.0529279E-4 33.929886 30-31 TCAACGC 785 0.0 33.288425 4 ATCAACG 835 0.0 31.297697 3 CAACGCA 845 0.0 30.91452 5 GTACATG 1660 0.0 30.64273 1 TATCAAC 915 0.0 29.080582 2 AACGCAG 930 0.0 28.59971 6 TATACTA 200 2.3646862E-11 28.49757 5 ACATGGG 1755 0.0 27.345158 3 TACATGG 1765 0.0 27.190228 2 GTATAAG 250 0.0 26.621948 1 ACGCAGA 1060 0.0 24.644123 7 GTATTAC 175 1.4719262E-7 24.448729 1 GTATAGA 235 1.7462298E-10 24.275333 1 CGCAGAG 1045 0.0 24.088854 8 CATGGGG 1215 0.0 23.071508 4 CCTTACG 105 0.0021908418 22.626474 2 GTATAGT 195 4.1812564E-7 21.941168 1 >>END_MODULE