##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031649_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2227161 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.862643517913614 33.0 31.0 34.0 30.0 34.0 2 32.09702172406934 34.0 31.0 34.0 30.0 34.0 3 32.22118652400972 34.0 31.0 34.0 30.0 34.0 4 35.336632600876186 37.0 35.0 37.0 33.0 37.0 5 35.13880451390806 37.0 35.0 37.0 32.0 37.0 6 35.34102114755063 37.0 35.0 37.0 33.0 37.0 7 35.43521954631928 37.0 36.0 37.0 33.0 37.0 8 35.49154102464977 37.0 36.0 37.0 33.0 37.0 9 37.381766742503125 39.0 38.0 39.0 35.0 39.0 10-11 37.33773669707758 39.0 38.0 39.0 35.0 39.0 12-13 37.4060094892107 39.0 38.0 39.0 35.0 39.0 14-15 38.83134896848499 41.0 39.0 41.0 36.0 41.0 16-17 38.79057373939288 41.0 39.0 41.0 36.0 41.0 18-19 38.77105786245359 41.0 39.0 41.0 35.0 41.0 20-21 38.80205853999778 41.0 39.0 41.0 35.0 41.0 22-23 38.73144644684422 41.0 39.0 41.0 35.0 41.0 24-25 38.669129892270924 41.0 39.0 41.0 34.5 41.0 26-27 38.60548025041746 41.0 39.0 41.0 34.5 41.0 28-29 38.52937663689334 41.0 39.0 41.0 34.0 41.0 30-31 38.40452531271875 40.0 38.0 41.0 34.0 41.0 32-33 38.293751776364616 40.0 38.0 41.0 34.0 41.0 34-35 38.26536833215022 40.0 38.0 41.0 34.0 41.0 36-37 38.16378586909523 40.0 38.0 41.0 34.0 41.0 38-39 37.99936241699635 40.0 38.0 41.0 33.0 41.0 40-41 37.94325915369387 40.0 38.0 41.0 33.0 41.0 42-43 37.86673931520892 40.0 38.0 41.0 33.0 41.0 44-45 37.72169546790735 40.0 38.0 41.0 33.0 41.0 46-47 37.6011309914281 40.0 38.0 41.0 32.5 41.0 48-49 37.787252695247446 40.0 38.0 41.0 33.0 41.0 50-51 37.60646985107947 39.5 38.0 40.5 33.0 41.0 52-53 37.819194481225196 40.0 38.0 41.0 33.0 41.0 54-55 38.18556471669538 41.0 39.0 41.0 34.0 41.0 56-57 38.16138954480614 41.0 39.0 41.0 34.0 41.0 58-59 38.063013181355096 41.0 39.0 41.0 33.0 41.0 60-61 37.89068684302572 40.5 38.0 41.0 33.0 41.0 62-63 37.62427592796389 40.0 38.0 41.0 32.0 41.0 64-65 37.43312248194001 40.0 38.0 41.0 32.0 41.0 66-67 37.248348233468526 40.0 37.0 41.0 31.0 41.0 68-69 36.96731264600987 39.0 37.0 41.0 31.0 41.0 70-71 36.59312977373436 39.0 36.0 41.0 31.0 41.0 72-73 36.17204414049995 38.5 35.5 40.5 30.0 41.0 74-75 35.613694070612766 37.0 35.0 40.0 29.0 41.0 76-77 35.06679422816761 37.0 35.0 39.0 28.5 41.0 78-79 34.48659391934395 36.0 34.5 39.0 27.0 40.5 80-81 33.86736679566498 36.0 34.0 37.5 26.0 39.0 82-83 33.27736207665274 35.0 34.0 37.0 24.5 39.0 84-85 32.73158788251051 35.0 33.5 37.0 23.5 38.5 86-87 32.254511910005604 35.0 33.0 36.0 20.5 37.0 88-89 31.76614443230642 35.0 32.5 36.0 19.5 37.0 90-91 31.329105304915092 35.0 32.0 35.5 18.5 36.5 92-93 30.905976936557348 35.0 31.0 35.0 16.0 36.0 94-95 30.498582500322158 35.0 31.0 35.0 11.0 36.0 96-97 30.167231062325534 35.0 30.5 35.0 10.0 36.0 98-99 29.841517743890094 35.0 29.5 35.0 10.0 35.5 100-101 29.128260372734616 34.5 27.5 35.0 9.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22187876073764912 1101 2 0.21926796248171598 1101 3 0.24002324399034336 1101 4 0.20700536870215558 1101 5 0.2201701940294214 1101 6 0.22609606903323254 1101 7 0.1362320070123033 1101 8 0.22822886162988 1101 9 0.1777677637104631 1101 10-11 0.28977309337686563 1101 12-13 0.2695504190949052 1101 14-15 0.37242460579239633 1101 16-17 0.3291821754225026 1101 18-19 0.33745487814731945 1101 20-21 0.14436472069919404 1101 22-23 0.18981878710805944 1101 24-25 0.31545687759107466 1101 26-27 0.25934801448912737 1101 28-29 0.2293619765547774 1101 30-31 0.12390872389094199 1101 32-33 0.30572028422031394 1101 34-35 0.2252927717609623 1101 36-37 0.14155847771558427 1101 38-39 0.1023868762618676 1101 40-41 0.03387973644293396 1101 42-43 0.15480808230297072 1101 44-45 0.18189869605725306 1101 46-47 0.3967539570375962 1101 48-49 0.24018225445198738 1101 50-51 0.08894876177488698 1101 52-53 0.20461047807793165 1101 54-55 0.1790713822033254 1101 56-57 0.13170133540803874 1101 58-59 0.25461227059908254 1101 60-61 0.15156947725552783 1101 62-63 0.07452132648717225 1101 64-65 0.2643129647932483 1101 66-67 0.21201706581259572 1101 68-69 0.12468578141257325 1101 70-71 0.057688894850436157 1101 72-73 0.20512369350546322 1101 74-75 0.15994019052288877 1101 76-77 0.04364825530609551 1101 78-79 0.23409113692612493 1101 80-81 0.1708983371382118 1101 82-83 0.17599316996280123 1101 84-85 0.01385152002149681 1101 86-87 -0.026573938701702104 1101 88-89 0.10975124578222406 1101 90-91 0.00941686057158364 1101 92-93 -0.0603515236610086 1101 94-95 -0.11862201827327112 1101 96-97 -0.11128289943157199 1101 98-99 -0.08911187696023504 1101 100-101 -0.09758144549248726 1102 1 -0.013656964178629494 1102 2 0.10990756928815415 1102 3 0.10471020489799088 1102 4 0.029071283444359608 1102 5 0.13064905359539125 1102 6 0.14783678658662325 1102 7 0.09669807718973544 1102 8 0.11182841704325597 1102 9 0.133037940361028 1102 10-11 0.16306557916952613 1102 12-13 0.11743895973735619 1102 14-15 0.060144699393710255 1102 16-17 0.1602116198004424 1102 18-19 0.2458244698878005 1102 20-21 0.1743664673829457 1102 22-23 0.0817108381269307 1102 24-25 0.14121879478256716 1102 26-27 0.150461417441214 1102 28-29 0.025064087817881386 1102 30-31 0.07272500219850997 1102 32-33 0.08473077151892028 1102 34-35 0.016663663374330895 1102 36-37 -0.03930408313213718 1102 38-39 -0.025668593703997544 1102 40-41 -0.0015903733495576944 1102 42-43 0.013612905766891004 1102 44-45 -0.158918392135881 1102 46-47 -0.6540843530360618 1102 48-49 -0.1149143005250508 1102 50-51 0.031242451928889636 1102 52-53 0.09241434053868858 1102 54-55 0.01202119742773533 1102 56-57 0.0922046943397774 1102 58-59 0.013118522353124717 1102 60-61 -0.21155380917902278 1102 62-63 -0.5329702132178298 1102 64-65 -0.15503358576800963 1102 66-67 -0.09049204212919193 1102 68-69 0.0015339461843879576 1102 70-71 -0.02020011680786382 1102 72-73 -0.1609704272355259 1102 74-75 -0.0794977698215007 1102 76-77 0.0010990063473954592 1102 78-79 -0.008128106557009573 1102 80-81 -0.1103148034188024 1102 82-83 -0.316799306344123 1102 84-85 -0.2293812252616405 1102 86-87 -0.23664046314775078 1102 88-89 -0.3442923271084588 1102 90-91 -0.2690594816763756 1102 92-93 -0.28125239379735945 1102 94-95 -0.22880080149542437 1102 96-97 -0.18790556536816538 1102 98-99 -0.3506883996988499 1102 100-101 -0.1207228791207946 1103 1 0.12857452564415794 1103 2 0.035701177583639776 1103 3 -0.03374459770002147 1103 4 0.09588242388420554 1103 5 0.07515033559013773 1103 6 0.030974303359649014 1103 7 0.17952942715855613 1103 8 0.24881412295933103 1103 9 0.3251028023654783 1103 10-11 0.25379048892591527 1103 12-13 0.22450599514812808 1103 14-15 0.31210265500582324 1103 16-17 0.2924879636900144 1103 18-19 0.28553512335863473 1103 20-21 0.1641158641839482 1103 22-23 0.12562341049505932 1103 24-25 0.24159394361365116 1103 26-27 0.11275659751895262 1103 28-29 0.150171655681703 1103 30-31 0.2386998536824052 1103 32-33 0.31381945279674994 1103 34-35 0.17134121177199546 1103 36-37 0.15441099274809034 1103 38-39 0.20857358045829244 1103 40-41 0.11768805575015051 1103 42-43 0.163649129205659 1103 44-45 0.1235037904680425 1103 46-47 0.34811425220404857 1103 48-49 0.06000963025353201 1103 50-51 0.21654644532677025 1103 52-53 0.1541551445100353 1103 54-55 0.2332449826825851 1103 56-57 0.2653996646365542 1103 58-59 0.24889342045501905 1103 60-61 0.29847945338736537 1103 62-63 0.2949788471776671 1103 64-65 0.3309243194462681 1103 66-67 0.17521318097569605 1103 68-69 0.27789736492103856 1103 70-71 0.225701909814795 1103 72-73 0.3779439405552196 1103 74-75 0.23167448365437338 1103 76-77 -0.07454876323372872 1103 78-79 0.13277918033892178 1103 80-81 0.23081265898756698 1103 82-83 0.06183012472740046 1103 84-85 0.04061064808862369 1103 86-87 0.12866976698132504 1103 88-89 0.24441761449305588 1103 90-91 0.16961360651352209 1103 92-93 -0.07036299813031022 1103 94-95 -0.11967627476252574 1103 96-97 0.03349146241994205 1103 98-99 0.06664152338146323 1103 100-101 0.09325054862446791 1104 1 -0.02692679195789438 1104 2 0.08550565772308971 1104 3 0.06539961903601466 1104 4 0.035530692868057656 1104 5 -0.0357098640536293 1104 6 -0.030747382820010216 1104 7 0.12766533466610497 1104 8 0.03373964407752794 1104 9 0.04611599593452809 1104 10-11 0.12346253616655645 1104 12-13 0.034731084280032576 1104 14-15 0.15757367214781226 1104 16-17 0.15137497748609263 1104 18-19 0.28619728785269416 1104 20-21 0.1288186067265471 1104 22-23 0.13080651477134353 1104 24-25 0.088877870038516 1104 26-27 0.1711765279682922 1104 28-29 0.12861504266479074 1104 30-31 0.23465360160948734 1104 32-33 0.05455909404632564 1104 34-35 0.059448573672383986 1104 36-37 0.12512322904488116 1104 38-39 0.1241657409454362 1104 40-41 0.14460077229168888 1104 42-43 0.06523083661904394 1104 44-45 0.15933730172396565 1104 46-47 0.4029680290862032 1104 48-49 0.35736565188501146 1104 50-51 0.22867335628846774 1104 52-53 0.25674793966897624 1104 54-55 0.14185209744119476 1104 56-57 0.10039771967458933 1104 58-59 0.09730513234327276 1104 60-61 0.3359593091384525 1104 62-63 0.22157539772182133 1104 64-65 0.4129995207425168 1104 66-67 0.2555108625225415 1104 68-69 0.22843405743278566 1104 70-71 0.21406650390269277 1104 72-73 0.11966015744334157 1104 74-75 0.10800166427845426 1104 76-77 0.19576424402843173 1104 78-79 0.20769560546958843 1104 80-81 0.24928584922239594 1104 82-83 0.25825080976347436 1104 84-85 0.255489022158919 1104 86-87 0.29383725306681185 1104 88-89 0.40604585475581345 1104 90-91 0.39883234600268125 1104 92-93 0.265978981753225 1104 94-95 0.06324315701610317 1104 96-97 0.21169516574450498 1104 98-99 0.42770499348608126 1104 100-101 0.43532914263632705 1105 1 0.1696197282840366 1105 2 0.13670463808642097 1105 3 0.1082301352270747 1105 4 0.10157903134034996 1105 5 -0.0804434804316756 1105 6 0.03672834890634391 1105 7 0.11084939428804574 1105 8 0.10600568467826577 1105 9 0.1667621944884985 1105 10-11 0.20679475697412641 1105 12-13 0.17105231669338394 1105 14-15 0.1849314525308685 1105 16-17 0.14643427329270509 1105 18-19 0.2498234170776925 1105 20-21 0.11077620886936046 1105 22-23 0.13880730053239887 1105 24-25 0.13966408064045766 1105 26-27 0.17901683756468145 1105 28-29 0.1790789386609788 1105 30-31 0.09322751069170465 1105 32-33 0.245568641696984 1105 34-35 0.08817625924372408 1105 36-37 0.12232456832609984 1105 38-39 0.18228399580392818 1105 40-41 0.25755378710480414 1105 42-43 0.2526292618819639 1105 44-45 0.21887831809662117 1105 46-47 0.3398326463260304 1105 48-49 0.2099398230692202 1105 50-51 0.1800977116660647 1105 52-53 0.028130571243771385 1105 54-55 0.11837775850626997 1105 56-57 0.2270823447774788 1105 58-59 0.2045955726773272 1105 60-61 0.2001050650888061 1105 62-63 0.10659299969569958 1105 64-65 0.11733921581600981 1105 66-67 0.01343965197372654 1105 68-69 0.06785408731023779 1105 70-71 0.035817127212276034 1105 72-73 0.07843031606953588 1105 74-75 0.06309667352422821 1105 76-77 0.061835850182326624 1105 78-79 0.09236977755289644 1105 80-81 0.19426568344425021 1105 82-83 0.09986437716496255 1105 84-85 0.13664443419713734 1105 86-87 0.18018988595211027 1105 88-89 0.10235942572646906 1105 90-91 0.16522087214762848 1105 92-93 0.08684235441729626 1105 94-95 0.10019531413998095 1105 96-97 0.12634255274844364 1105 98-99 0.09204920586824983 1105 100-101 0.11897613103817761 1106 1 0.005862180927927341 1106 2 0.013815365385006828 1106 3 -0.04554561480183139 1106 4 0.11153601614280717 1106 5 0.1047083466398604 1106 6 -0.023531809136642323 1106 7 0.08001765799453153 1106 8 0.10064183074576505 1106 9 0.1241488575262224 1106 10-11 0.19674491705328023 1106 12-13 0.1692367816995457 1106 14-15 0.16343856221450181 1106 16-17 0.16226543147995898 1106 18-19 0.1629740807743545 1106 20-21 0.16226206982303637 1106 22-23 0.15590223478687903 1106 24-25 0.11924516336694779 1106 26-27 0.14914434836196477 1106 28-29 0.15200540456959288 1106 30-31 0.1962333817954942 1106 32-33 0.2204772957940193 1106 34-35 0.340526926214622 1106 36-37 0.16800621698279627 1106 38-39 0.24013311825910932 1106 40-41 0.3086077310684061 1106 42-43 0.17988239260866834 1106 44-45 0.23596685737049938 1106 46-47 0.4103389701944522 1106 48-49 0.3770977095127819 1106 50-51 0.21074808630960717 1106 52-53 0.21039148338287106 1106 54-55 0.2768880963761191 1106 56-57 0.2716777420285581 1106 58-59 0.30541973219781937 1106 60-61 0.25795354804592563 1106 62-63 0.2705360014893685 1106 64-65 0.43406239705795 1106 66-67 0.29130667525861753 1106 68-69 0.30093565452046533 1106 70-71 0.1552146126035865 1106 72-73 0.23086810825619608 1106 74-75 0.33391655608168236 1106 76-77 0.33445604767978665 1106 78-79 0.2528496415495667 1106 80-81 0.13298168900634977 1106 82-83 0.1531166596614426 1106 84-85 0.0918592339216886 1106 86-87 0.0016048086649291804 1106 88-89 0.06580750718112682 1106 90-91 0.1326601888976704 1106 92-93 0.16785767781402328 1106 94-95 -0.008293741234410845 1106 96-97 0.015498865515180427 1106 98-99 0.11585810236062599 1106 100-101 0.08063425742141561 1107 1 0.1158552642530708 1107 2 0.08034017092439427 1107 3 0.16387372467462313 1107 4 0.18700903355166787 1107 5 0.2425410462596318 1107 6 0.19773847788646037 1107 7 0.1470923958790138 1107 8 0.23678126788717435 1107 9 0.2429399134719219 1107 10-11 0.17961764267672464 1107 12-13 0.254532989245142 1107 14-15 0.20113774733193424 1107 16-17 0.13217033664970756 1107 18-19 0.2495562984946531 1107 20-21 0.1888222902495258 1107 22-23 0.2577497995881828 1107 24-25 0.13545304913220946 1107 26-27 0.15894013637154814 1107 28-29 0.29278241550776585 1107 30-31 0.27102134602532146 1107 32-33 0.2679250238742057 1107 34-35 0.25557277280806545 1107 36-37 0.2641782343084955 1107 38-39 0.27120146105404785 1107 40-41 0.33923055307175076 1107 42-43 0.23606289514478362 1107 44-45 0.1907850017362378 1107 46-47 0.37270829039491815 1107 48-49 0.2807971856597007 1107 50-51 0.013776399321272947 1107 52-53 0.14496559763274064 1107 54-55 0.11924271391801966 1107 56-57 0.11746282242072681 1107 58-59 0.21045860542235317 1107 60-61 0.2748417624855293 1107 62-63 0.22691512220235666 1107 64-65 0.29536983758588065 1107 66-67 0.12607893523487945 1107 68-69 0.19716663366460097 1107 70-71 0.16225790418489083 1107 72-73 0.1654762389397817 1107 74-75 0.20104458637899114 1107 76-77 0.10351564447166339 1107 78-79 0.12234417624254235 1107 80-81 0.19963263882077342 1107 82-83 0.11784681485196558 1107 84-85 0.14206985234045533 1107 86-87 0.1126689372016969 1107 88-89 0.12987887743866722 1107 90-91 0.25060036154996723 1107 92-93 0.18229859882786315 1107 94-95 0.1272929288590916 1107 96-97 0.004966999675993122 1107 98-99 0.06247450659680709 1107 100-101 0.17101875651052723 1108 1 0.03890383817700638 1108 2 0.08655041679349296 1108 3 0.061381730638288445 1108 4 0.04025949891208569 1108 5 0.12245776659345609 1108 6 0.06520537333695842 1108 7 0.02460512161189854 1108 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-1.4576084394521303 2316 58-59 -1.7092778430787376 2316 60-61 -1.2477395366190294 2316 62-63 -1.0320525633504616 2316 64-65 -1.4319017659484103 2316 66-67 -1.6718374121027821 2316 68-69 -1.884508249028336 2316 70-71 -1.4688330390676683 2316 72-73 -1.4658650170491825 2316 74-75 -1.3982547851815283 2316 76-77 -1.0885669327729914 2316 78-79 -1.3825140540402288 2316 80-81 -1.336294655527226 2316 82-83 -1.3582371938107052 2316 84-85 -1.6572562810508948 2316 86-87 -1.2477365941535297 2316 88-89 -0.9903080103767259 2316 90-91 -1.1506500278253995 2316 92-93 -1.2719559760236798 2316 94-95 -1.3060268484429187 2316 96-97 -1.1005864819578512 2316 98-99 -0.6126625164492694 2316 100-101 -1.1562636012005854 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 15.0 7 524.0 8 3990.0 9 5426.0 10 4655.0 11 4783.0 12 5856.0 13 6349.0 14 6234.0 15 5875.0 16 5767.0 17 5749.0 18 5921.0 19 6392.0 20 6838.0 21 6909.0 22 7020.0 23 7336.0 24 8382.0 25 10170.0 26 12545.0 27 15153.0 28 19232.0 29 24152.0 30 30993.0 31 39805.0 32 51813.0 33 68644.0 34 94671.0 35 129107.0 36 190186.0 37 363808.0 38 748456.0 39 324371.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.413791843479096 27.793222943130264 15.073554122761474 21.71943109062916 2 12.440280865813664 24.44422113451259 43.757711327551505 19.357786672122238 3 14.098966285745648 32.84217885263026 33.79068748431074 19.26816737731335 4 9.538318647747078 20.252519184411096 47.586249413319045 22.622912754522787 5 8.811284118498007 43.15856656623496 39.25874381453832 8.771405500728708 6 22.971942511097346 47.30687785700115 20.098487108095135 9.622692523806373 7 22.027140764092536 34.88879682123686 30.097350295973506 12.986712118697097 8 21.183254782866477 40.965646672697595 24.47184801350615 13.379250530929781 9 25.0945895795144 17.545236783212065 25.69192879072435 31.668244846549182 10-11 19.811312478278328 29.820254215269788 34.60380231900294 15.76463098744895 12-13 20.323139828353614 29.32327275679875 32.42822032653614 17.925367088311496 14-15 19.96179539164785 31.181992186770685 28.878153527909 19.97805889367246 16-17 18.06927094626553 33.814058885584636 30.568554733799274 17.54811543435056 18-19 17.92516327708066 33.04048525986551 32.029302624223874 17.005048838829953 20-21 18.14867971783752 32.91131539251956 31.548501418737303 17.391503470905615 22-23 19.146370648552125 31.52764887675386 31.36378555479375 17.962194919900266 24-25 18.94735046096802 32.88464551956504 29.952886208046927 18.215117811420008 26-27 18.6313427722558 32.281343827410765 31.637115592451554 17.450197807881874 28-29 18.458252456827324 30.380785223879194 33.26679121985344 17.894171099440047 30-31 19.153891433982544 31.74510060116893 31.115824136647507 17.985183828201016 32-33 18.266057101395006 31.64403022502639 32.060457236814045 18.029455436764565 34-35 18.31533508354358 32.22250210020739 31.26680109789997 18.195361718349055 36-37 19.316632250654532 30.208727613315787 30.873744646211026 19.600895489818654 38-39 17.890332131354672 31.76368928874024 32.21028924267262 18.13568933723247 40-41 17.411673426393513 30.547746660434516 33.98820291842395 18.052376994748023 42-43 17.932470979870786 29.775687523263922 31.96338297949722 20.328458517368077 44-45 18.174421843522705 30.40162566569855 32.20579265928037 19.218159831498376 46-47 18.261101381197758 29.800328679847603 32.71858278468996 19.219987154264683 48-49 16.803094792513235 30.392423627249794 32.404861126870635 20.39962045336634 50-51 16.594537729705298 29.981428811154125 31.959225603664727 21.46480785547585 52-53 16.890158066022074 29.965932613793473 32.979853872390635 20.164055447793817 54-55 17.867479705849973 30.051743623042203 31.815408051154964 20.265368619952863 56-57 18.39394698782183 29.897861600164514 30.901557641500847 20.806633770512807 58-59 17.783059496183387 28.927085844952227 31.61155655167656 21.678298107187825 60-61 18.943471985539766 30.07803401932528 30.744819089670557 20.233674905464405 62-63 17.956752078240267 29.664616725887427 31.70933644616469 20.66929474970761 64-65 18.262973911675115 29.213708453696313 30.70149606033698 21.82182157429159 66-67 18.814767370650664 29.7710731682651 30.023585992235386 21.390573468848846 68-69 18.77018907979936 29.41923547762815 30.18422082316271 21.626354619409792 70-71 19.046695640880333 28.943662066774312 30.897241907121032 21.11240038522432 72-73 19.339070834026987 29.663718834158193 29.55149855079692 21.445711781017902 74-75 19.467029851705934 29.62623655198431 28.810104373732877 22.096629222576883 76-77 19.0834669379707 30.593249587261788 30.13760640186325 20.18567707290426 78-79 20.278308834770034 29.99085912531766 28.813426339747384 20.917405700164924 80-81 20.65915892974304 30.276417137772665 28.42234165895221 20.642082273532083 82-83 20.894810058518377 30.360325157508424 28.79167858507904 19.953186198894166 84-85 21.43118783624049 29.390418747219066 28.46348490092001 20.714908515620436 86-87 20.224193032020356 30.008166566503597 29.838162691035315 19.929477710440736 88-89 21.755766102492462 28.82155761338558 29.18705477154124 20.23562151258072 90-91 21.949659892078895 29.194754264609895 29.1101543297006 19.745431513610608 92-93 23.06998666513754 29.312501264900526 28.414702978868856 19.202809091093076 94-95 22.66369242645533 27.882984943639528 28.646829356677056 20.80649327322808 96-97 23.319832416357045 27.363703468181466 28.560292923085218 20.756171192376268 98-99 22.863634760254236 28.051062062959648 28.297308877309995 20.78799429947612 100-101 23.312922300143278 27.985736987460896 28.41114281568024 20.29019789671559 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 56.0 1 40.5 2 40.0 3 70.0 4 64.0 5 58.5 6 91.0 7 156.5 8 221.5 9 244.0 10 217.5 11 198.5 12 216.5 13 241.0 14 277.5 15 343.0 16 408.0 17 441.0 18 529.5 19 629.0 20 716.0 21 796.0 22 895.5 23 1065.5 24 1216.5 25 1480.5 26 2007.0 27 3523.5 28 5687.0 29 9328.5 30 16396.5 31 26183.0 32 49730.5 33 77478.5 34 104923.5 35 138466.0 36 187406.5 37 226966.5 38 217020.0 39 196978.5 40 174029.5 41 146550.5 42 127212.5 43 108274.5 44 92991.0 45 77452.5 46 59737.5 47 43026.5 48 32510.0 49 25006.0 50 18578.0 51 13617.5 52 9089.5 53 6002.5 54 3988.5 55 2850.0 56 2279.5 57 1901.5 58 1561.5 59 1290.0 60 1135.5 61 990.0 62 809.5 63 640.0 64 506.0 65 413.0 66 349.5 67 302.5 68 246.5 69 219.5 70 182.0 71 130.5 72 106.0 73 83.5 74 62.0 75 55.5 76 46.0 77 32.0 78 30.0 79 28.0 80 17.5 81 13.0 82 10.0 83 4.0 84 4.5 85 5.0 86 3.0 87 1.5 88 0.0 89 1.5 90 1.5 91 0.0 92 0.0 93 0.0 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46507639097487785 2 0.3602343970642446 3 0.1932056101916296 4 0.2206396394333414 5 0.24331424625341408 6 0.19450771632585162 7 0.1866950795205196 8 0.18741348290491797 9 0.18570727486697192 10-11 0.19928958885325307 12-13 0.18680733004933187 14-15 0.19722417912310786 16-17 0.08591655475288945 18-19 0.03625692080635392 20-21 2.0205095186203424E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.13862940308311794 46-47 0.2080451301006079 48-49 0.08416544650341848 50-51 0.06292764645214244 52-53 0.07058313251713728 54-55 0.07929377355296721 56-57 0.11150967532208043 58-59 0.09363939113517164 60-61 0.1405825622844509 62-63 0.24198969001342965 64-65 0.13407203161334091 66-67 0.104325641478097 68-69 0.1083217603038128 70-71 0.08818401543489672 72-73 0.07307509425676904 74-75 0.14071726291902562 76-77 0.21588021701170235 78-79 0.1385171525543057 80-81 0.08535530210882823 82-83 0.08811666511760936 84-85 0.09837636345104822 86-87 0.07289549341066945 88-89 0.09471699621176916 90-91 0.11552824425355868 92-93 0.16460417545027056 94-95 0.132522974315732 96-97 0.08995757379013013 98-99 0.07864272048585622 100-101 0.06303989698095468 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2227161.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.984234585836454 #Duplication Level Percentage of deduplicated Percentage of total 1 74.05219542675373 9.615110770171759 2 9.6246193796442 2.4993663164937603 3 4.003453323309933 1.5594533130990815 4 2.2268018995943026 1.1565327296207466 5 1.413491614775464 0.9176553355678698 6 1.0315307483039846 0.8036182331089412 7 0.7588050404601908 0.6896751855175167 8 0.576152305313414 0.5984717351487867 9 0.4923250603430557 0.575321766838229 >10 3.9832623890578107 10.823823297185713 >50 0.736372230826835 6.7658670378793415 >100 0.8612414983875534 24.281491953531013 >500 0.1380697845385167 12.456975293685705 >1k 0.09668452612387086 22.764388001412122 >5k 0.004638003098193067 3.9366878796627827 >10k+ 3.567694690917744E-4 0.5555611510766486 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 11993 0.5384882368180837 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 8430 0.37850878315487746 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 8233 0.36966344148447283 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 8029 0.36050379833339397 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 7939 0.3564627792961533 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 6674 0.2996640117171592 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 6165 0.2768098040509869 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 6037 0.2710625769758001 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 5956 0.2674256598422835 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 5797 0.26028652620982495 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 5659 0.25409029701938923 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 5551 0.24924107417470046 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 5376 0.24138353715784355 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 5136 0.2306074863918684 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 4887 0.21942733372216916 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 4846 0.21758642504964842 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 4511 0.20254485418880808 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 4324 0.19414851463365246 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 4284 0.1923525061726566 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 4062 0.18238465921412955 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 4021 0.18054375054160882 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 3951 0.17740073573486603 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 3935 0.1766823323504677 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 3652 0.163975572488922 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 3639 0.16339186973909833 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 3639 0.16339186973909833 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 3632 0.16307756825842407 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 3570 0.16029375514388047 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 3555 0.15962025197100704 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 3549 0.15935085070185767 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 3496 0.15697113949103814 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 3468 0.15571393356834104 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 3376 0.15158311410805056 No Hit ATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTA 3369 0.1512688126273763 No Hit CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC 3359 0.15081981051212734 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 3352 0.15050550903145304 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 3259 0.14632978935963767 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 3177 0.14264797201459617 No Hit ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG 3172 0.14242347095697167 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 3146 0.14125606545732436 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 3142 0.14107646461122478 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 3087 0.13860695297735548 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 3054 0.13712524599703388 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 2968 0.1332638278058928 No Hit GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT 2967 0.1332189275943679 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 2946 0.13227602315234507 No Hit CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC 2930 0.13155761976794675 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 2926 0.13137801892184714 No Hit ACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAG 2902 0.13030041384524962 No Hit GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT 2868 0.12877380665340313 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 2845 0.12774110178833054 No Hit AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT 2820 0.1266185965002081 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 2816 0.12643899565410854 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 2769 0.1243286857124384 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 2736 0.12284697873211682 No Hit CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA 2711 0.1217244734439944 No Hit GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT 2707 0.1215448725978948 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 2667 0.11974886413689895 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 2597 0.11660584933015619 No Hit ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC 2594 0.1164711486955815 No Hit GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCT 2590 0.11629154784948191 No Hit GCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGT 2590 0.11629154784948191 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 2584 0.11602214658033255 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 2581 0.11588744594575787 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 2571 0.11543844383050889 No Hit GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA 2549 0.11445063917696116 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 2508 0.1126097305044404 No Hit CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT 2402 0.10785030808280137 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2401 0.10780540787127647 No Hit CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG 2398 0.10767070723670179 No Hit TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT 2389 0.10726660533297773 No Hit CTACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTC 2379 0.10681760321772876 No Hit AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA 2359 0.10591959898723083 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 2350 0.10551549708350677 No Hit GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG 2285 0.10259698333438848 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 2278 0.10228268185371421 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 2258 0.10138467762321629 No Hit ACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCAT 2256 0.10129487720016649 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 2237 0.10044177318119345 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.6940126914937895E-4 0.0 2 0.0 0.0 0.0 2.6940126914937895E-4 0.0 3 0.0 0.0 0.0 4.041019037240684E-4 0.0 4 0.0 0.0 0.0 6.735031728734474E-4 0.0 5 0.0 0.0 0.0 7.184033843983439E-4 0.0 6 0.0 0.0 0.0 8.531040189730334E-4 0.0 7 0.0 0.0 0.0 0.0012123057111722054 0.0 8 0.0 0.0 0.0 0.001526607191846481 0.0 9 0.0 0.0 0.0 0.003187915018267651 0.0 10-11 0.0 0.0 0.0 0.007251384161270784 0.0 12-13 4.4900211524896496E-5 0.0 0.0 0.013761914832380776 0.0 14-15 4.4900211524896496E-5 0.0 0.0 0.028399383789497034 0.0 16-17 4.4900211524896496E-5 0.0 0.0 0.0608622367219972 0.0 18-19 4.4900211524896496E-5 0.0 0.0 0.10131732730592893 0.0 20-21 4.4900211524896496E-5 0.0 0.0 0.1567690885391761 0.0 22-23 4.4900211524896496E-5 0.0 0.0 0.23453625490029684 0.0 24-25 4.4900211524896496E-5 0.0 0.0 0.333810622581843 0.0 26-27 4.4900211524896496E-5 0.0 0.0 0.43083997968714427 0.0 28-29 4.4900211524896496E-5 0.0 0.0 0.5343125171462683 0.0 30-31 4.4900211524896496E-5 0.0 0.0 0.7154175203319383 0.0 32-33 4.4900211524896496E-5 0.0 0.0 1.0785928812510637 0.0 34-35 4.4900211524896496E-5 0.0 0.0 1.615307559713914 0.0 36-37 4.4900211524896496E-5 0.0 0.0 2.366847300217631 0.0 38-39 4.4900211524896496E-5 0.0 0.0 3.511443492410293 0.0 40-41 4.4900211524896496E-5 0.0 0.0 4.741417436817545 0.0 42-43 4.4900211524896496E-5 0.0 0.0 5.838307154265003 0.0 44-45 4.4900211524896496E-5 0.0 0.0 7.084108423234782 0.0 46-47 4.4900211524896496E-5 0.0 0.0 8.509330937458046 0.0 48-49 4.4900211524896496E-5 0.0 0.0 9.89355955855908 0.0 50-51 4.4900211524896496E-5 0.0 0.0 11.27994338981331 0.0 52-53 4.4900211524896496E-5 0.0 0.0 12.521928140803471 0.0 54-55 4.4900211524896496E-5 0.0 0.0 13.70556506691703 0.0 56-57 4.4900211524896496E-5 0.0 0.0 15.033331672025508 0.0 58-59 4.4900211524896496E-5 0.0 0.0 16.428785346007764 0.0 60-61 4.4900211524896496E-5 0.0 0.0 17.746067751725178 0.0 62-63 4.4900211524896496E-5 0.0 0.0 18.885388169063667 0.0 64-65 4.4900211524896496E-5 0.0 0.0 20.094281464159977 0.0 66-67 4.4900211524896496E-5 0.0 0.0 21.322167548731322 0.0 68-69 4.4900211524896496E-5 0.0 0.0 22.53411405821133 0.0 70-71 4.4900211524896496E-5 0.0 0.0 23.902380654115262 0.0 72-73 4.4900211524896496E-5 0.0 0.0 25.090664752121647 0.0 74-75 4.4900211524896496E-5 0.0 0.0 26.210543377869854 0.0 76-77 4.4900211524896496E-5 0.0 0.0 27.3786448307958 0.0 78-79 4.4900211524896496E-5 4.4900211524896496E-5 0.0 28.591938346621554 0.0 80-81 4.4900211524896496E-5 8.980042304979299E-5 0.0 29.896558892688944 0.0 82-83 4.4900211524896496E-5 1.3470063457468947E-4 0.0 31.129271749999212 0.0 84-85 4.4900211524896496E-5 1.7960084609958598E-4 0.0 32.19354146377384 0.0 86-87 4.4900211524896496E-5 1.7960084609958598E-4 0.0 33.26248079954705 0.0 88-89 4.4900211524896496E-5 2.469511633869307E-4 0.0 34.393741628916814 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCAGG 15 6.564151E-4 95.19817 1 GGTACCG 50 2.830273E-5 47.493134 8 TAACTTT 1840 0.0 45.687355 6 GACATAT 560 0.0 42.499184 1 AACTTGC 295 0.0 41.858353 7 CATTCCG 600 0.0 41.160713 9 ACATATA 590 0.0 40.296436 2 GGAGTGA 595 0.0 39.91109 6 AACTTTA 2255 0.0 38.96332 7 GTATGGT 205 0.0 37.150505 1 GGCATTC 695 0.0 36.90114 7 GTAAGGT 130 2.055458E-10 36.542213 4 TAAGTGA 65 1.3295391E-4 36.534 6 TGGGCCA 130 2.055458E-10 36.534 6 ATGTATT 275 0.0 36.348392 1 ACTTTAT 2340 0.0 36.330215 8 ACCCTTG 1310 0.0 36.254303 7 TGGGTAT 280 0.0 35.62065 6 GGGGAGC 320 0.0 35.61985 7 TGGGGAG 335 0.0 35.44343 6 >>END_MODULE