##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031648_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1545527 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21605445909389 34.0 31.0 34.0 31.0 34.0 2 32.42585085863916 34.0 31.0 34.0 31.0 34.0 3 32.54369803956838 34.0 31.0 34.0 31.0 34.0 4 35.68121682765814 37.0 35.0 37.0 35.0 37.0 5 35.49875738178628 37.0 35.0 37.0 33.0 37.0 6 35.71301763087931 37.0 36.0 37.0 35.0 37.0 7 35.81254096499123 37.0 37.0 37.0 35.0 37.0 8 35.87219505062028 37.0 37.0 37.0 35.0 37.0 9 37.7846300970478 39.0 39.0 39.0 35.0 39.0 10-11 37.73643618002144 39.0 38.5 39.0 35.0 39.0 12-13 37.805482531201335 39.0 39.0 39.0 35.0 39.0 14-15 39.23563936443686 41.0 39.0 41.0 36.0 41.0 16-17 39.195498687502706 41.0 39.0 41.0 36.0 41.0 18-19 39.19581993714765 41.0 39.0 41.0 36.0 41.0 20-21 39.246472238919154 41.0 39.0 41.0 36.0 41.0 22-23 39.14857035820144 41.0 39.0 41.0 36.0 41.0 24-25 39.072709826486374 41.0 39.0 41.0 35.5 41.0 26-27 38.98070593396298 41.0 39.0 41.0 35.0 41.0 28-29 38.89224678701828 41.0 39.0 41.0 35.0 41.0 30-31 38.69889008732943 40.0 38.0 41.0 35.0 41.0 32-33 38.53207255518668 40.0 38.0 41.0 35.0 41.0 34-35 38.443268865571426 40.0 38.0 41.0 34.5 41.0 36-37 38.28645989361557 40.0 38.0 41.0 34.0 41.0 38-39 38.095849182835366 40.0 38.0 41.0 34.0 41.0 40-41 38.010558857917076 40.0 38.0 41.0 33.5 41.0 42-43 37.86648955340152 40.0 38.0 41.0 33.0 41.0 44-45 37.62710195292609 40.0 38.0 41.0 33.0 41.0 46-47 37.412327639698304 40.0 38.0 41.0 32.0 41.0 48-49 37.50117338616536 40.0 38.0 41.0 32.5 41.0 50-51 37.28663523833618 39.5 37.5 40.5 32.5 41.0 52-53 37.44391621757498 40.0 38.0 41.0 32.5 41.0 54-55 37.768038669010636 40.0 38.0 41.0 33.0 41.0 56-57 37.71057348076093 41.0 38.0 41.0 33.0 41.0 58-59 37.58715538453873 40.0 38.0 41.0 32.0 41.0 60-61 37.37996489223417 40.0 38.0 41.0 31.5 41.0 62-63 37.1023246439564 40.0 37.5 41.0 31.0 41.0 64-65 36.89821594834642 40.0 37.0 41.0 30.5 41.0 66-67 36.70450823570213 39.5 36.5 41.0 30.0 41.0 68-69 36.45401050903672 39.0 36.0 41.0 30.0 41.0 70-71 36.13689408208333 39.0 36.0 41.0 30.0 41.0 72-73 35.764987606169285 38.0 35.0 40.0 29.0 41.0 74-75 35.271953838399455 37.0 35.0 39.5 29.0 41.0 76-77 34.819459640627436 37.0 35.0 39.0 28.0 41.0 78-79 34.40189559936513 36.0 35.0 39.0 28.0 40.5 80-81 33.9761709112814 36.0 34.5 37.5 26.5 39.0 82-83 33.573016517990304 35.5 34.0 37.0 26.0 39.0 84-85 33.16730636216643 35.0 34.0 36.5 26.0 38.0 86-87 32.88796539950451 35.0 34.0 36.0 26.0 37.0 88-89 32.59166679068046 35.0 34.0 36.0 25.5 37.0 90-91 32.36603954508721 35.0 34.0 36.0 25.0 36.5 92-93 32.180567534569114 35.0 34.0 35.0 25.0 36.0 94-95 31.989673425310592 35.0 34.0 35.0 24.0 36.0 96-97 31.84552130114841 35.0 33.0 35.0 23.5 36.0 98-99 31.72466899640058 35.0 33.0 35.0 23.0 36.0 100-101 31.29909441892636 34.5 32.0 35.0 19.5 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10428025052846834 1101 2 0.08414898464288001 1101 3 0.16455113950625844 1101 4 0.08630830141162704 1101 5 0.015168878592241697 1101 6 0.22001478127107532 1101 7 0.1072115368013229 1101 8 0.2652411668193011 1101 9 0.20939378159690847 1101 10-11 0.14875906957066576 1101 12-13 0.06327745477668856 1101 14-15 0.1452820755325206 1101 16-17 0.10260289015425172 1101 18-19 0.1855679942643107 1101 20-21 -0.052911493235811236 1101 22-23 0.05323841629725479 1101 24-25 0.16842162121670867 1101 26-27 0.04223700942288389 1101 28-29 0.08957977950287699 1101 30-31 0.0038838376546053155 1101 32-33 0.15763833038941044 1101 34-35 0.012725239626966811 1101 36-37 0.13492796915492278 1101 38-39 -0.003486112320381096 1101 40-41 -0.005033968377873066 1101 42-43 0.0744792755370085 1101 44-45 4.5705742316926035E-4 1101 46-47 0.07522576213884236 1101 48-49 -0.029840400932577893 1101 50-51 0.07564497803759451 1101 52-53 -0.028039085297528743 1101 54-55 0.01036668481577152 1101 56-57 0.10098173246267095 1101 58-59 0.11139342381382278 1101 60-61 0.05808849257751092 1101 62-63 0.052244870733360926 1101 64-65 0.1477925555330799 1101 66-67 0.018596337205522673 1101 68-69 0.01298200278331052 1101 70-71 0.04825417294630796 1101 72-73 0.07772165758751726 1101 74-75 0.01396456868629059 1101 76-77 -0.0022586277301996915 1101 78-79 0.10686482787112794 1101 80-81 0.22073772393673607 1101 82-83 0.23257096422914003 1101 84-85 0.1777245105443228 1101 86-87 0.18708856988968137 1101 88-89 0.2497817926174335 1101 90-91 0.11065263266146985 1101 92-93 0.04193081735448345 1101 94-95 -0.05515358840864337 1101 96-97 -0.02035666698736094 1101 98-99 -0.1147304981273507 1101 100-101 0.08811127492334947 1102 1 0.044430117253163814 1102 2 0.12901380540118623 1102 3 0.12204719564508082 1102 4 -0.11081811973834732 1102 5 0.1650192838579727 1102 6 0.13460706762259633 1102 7 0.1389596192688174 1102 8 0.23355700210189667 1102 9 0.22372359718740142 1102 10-11 0.12438668747851978 1102 12-13 0.11086353974649654 1102 14-15 0.12353032119427354 1102 16-17 0.268961754322234 1102 18-19 0.3755465070206583 1102 20-21 0.24355127127488174 1102 22-23 0.07391240857273118 1102 24-25 0.24785438518341607 1102 26-27 0.18678768123923106 1102 28-29 0.1754457970190586 1102 30-31 0.10846619635557886 1102 32-33 0.2756556561795449 1102 34-35 0.1050272305968818 1102 36-37 0.09015426518930525 1102 38-39 0.09670564092990475 1102 40-41 0.31186207688134004 1102 42-43 0.23635709743767563 1102 44-45 0.02601327784420704 1102 46-47 -0.4570506617310386 1102 48-49 0.09187803457848531 1102 50-51 0.014745641694240419 1102 52-53 0.1375962843374623 1102 54-55 0.13024714502357426 1102 56-57 0.13121550823376538 1102 58-59 0.25486905347166555 1102 60-61 0.14813772284460924 1102 62-63 -0.12500585004126918 1102 64-65 0.11662789252921613 1102 66-67 0.017898001786932127 1102 68-69 0.18222600539442624 1102 70-71 0.2530024458071054 1102 72-73 0.24120150309695276 1102 74-75 0.14148863983898963 1102 76-77 0.259739549933947 1102 78-79 0.24461777896724612 1102 80-81 0.13169893810199795 1102 82-83 0.16273334251342675 1102 84-85 0.16096378052201032 1102 86-87 0.0887313920623356 1102 88-89 0.14350561353894165 1102 90-91 0.26090898363791837 1102 92-93 0.3188741889478024 1102 94-95 0.2366200611588951 1102 96-97 0.1570912468460719 1102 98-99 -0.007446594520754246 1102 100-101 0.18058325990947566 1103 1 0.041806028568451836 1103 2 0.058105985566974994 1103 3 0.07252157894679101 1103 4 0.26025198912013536 1103 5 0.07299391683510237 1103 6 0.153537458114954 1103 7 0.21994184237878045 1103 8 0.06537064436695061 1103 9 0.16033849781658205 1103 10-11 0.20753234811968468 1103 12-13 0.2764083315171959 1103 14-15 0.3921423755236475 1103 16-17 0.24254110117132655 1103 18-19 0.22893709925075711 1103 20-21 0.05097661832371614 1103 22-23 0.18426395156684094 1103 24-25 0.14989797851208664 1103 26-27 0.06208479499135677 1103 28-29 0.15461287981143101 1103 30-31 0.2026899554687347 1103 32-33 0.15080421305560776 1103 34-35 0.07805853989479061 1103 36-37 0.01874210100274354 1103 38-39 -0.07256930164474795 1103 40-41 -0.15830171019546668 1103 42-43 -0.19066777023434867 1103 44-45 -0.19210816428103072 1103 46-47 -0.08889551342384294 1103 48-49 -0.18240789371472488 1103 50-51 -0.04254113549541927 1103 52-53 -0.17645794516418079 1103 54-55 -0.16071335632467765 1103 56-57 0.026834952955624658 1103 58-59 -0.04162175847281446 1103 60-61 0.0374066732091336 1103 62-63 -0.004784637617419207 1103 64-65 0.08384800638120282 1103 66-67 -0.16498050615275162 1103 68-69 -0.12653136003052623 1103 70-71 -0.12299750425781042 1103 72-73 -0.11252615095278173 1103 74-75 -0.11420645653573303 1103 76-77 -0.4601183847509631 1103 78-79 -0.22616395477888318 1103 80-81 -0.23385833743979845 1103 82-83 -0.39477190067043466 1103 84-85 -0.2722243249408791 1103 86-87 -0.16874888357932605 1103 88-89 -0.07401910432783154 1103 90-91 -0.29519682880590636 1103 92-93 -0.25930417917363613 1103 94-95 -0.2544465522436816 1103 96-97 -0.17299464149908061 1103 98-99 -0.10787353949229228 1103 100-101 -0.10020546474846981 1104 1 0.1310843265371915 1104 2 0.20112729871354418 1104 3 0.3086227267229731 1104 4 0.24381405964795988 1104 5 0.18353816662745004 1104 6 0.21611768918042884 1104 7 0.32941005199895557 1104 8 0.34399661657868563 1104 9 0.39063941150683235 1104 10-11 0.4758545277617543 1104 12-13 0.38480876004548037 1104 14-15 0.4315358286687072 1104 16-17 0.3823272145897363 1104 18-19 0.4410338201599231 1104 20-21 0.3836476520773999 1104 22-23 0.3978650049535304 1104 24-25 0.3401635905335354 1104 26-27 0.3677450966601654 1104 28-29 0.4053361392692594 1104 30-31 0.4020735921069516 1104 32-33 0.2935352785241534 1104 34-35 0.3365771912577813 1104 36-37 0.3972156341900899 1104 38-39 0.3936593191360416 1104 40-41 0.3579380013502629 1104 42-43 0.30672000066348204 1104 44-45 0.4112365272541254 1104 46-47 0.5138285672225393 1104 48-49 0.3997876529891897 1104 50-51 0.3881179863047848 1104 52-53 0.38283871156011884 1104 54-55 0.36238965092444175 1104 56-57 0.35439361602290376 1104 58-59 0.12546061523405427 1104 60-61 0.32120518940637055 1104 62-63 0.3444104560169521 1104 64-65 0.6345788169981432 1104 66-67 0.4266091544226498 1104 68-69 0.34607091098057197 1104 70-71 0.49228745760883186 1104 72-73 0.3273418453471493 1104 74-75 0.38017618901286454 1104 76-77 0.41164995512670544 1104 78-79 0.5182458277477409 1104 80-81 0.5247629624922467 1104 82-83 0.46143515211216624 1104 84-85 0.48324200632846726 1104 86-87 0.5121037366728842 1104 88-89 0.3496351375357918 1104 90-91 0.1964567806481483 1104 92-93 0.21110471609178205 1104 94-95 0.23513992740594603 1104 96-97 0.14052948445425528 1104 98-99 0.2064646958018237 1104 100-101 0.23906575400861385 1105 1 -0.1258023305646745 1105 2 -0.06998998966522407 1105 3 -0.06644900928850461 1105 4 0.031188521627875332 1105 5 -0.19956800266953678 1105 6 -0.11399350163736699 1105 7 0.06943874640354863 1105 8 -0.020736166778561937 1105 9 0.10851961236766527 1105 10-11 0.12380556793392117 1105 12-13 0.1389470312076 1105 14-15 0.19142643125120173 1105 16-17 0.18456896494841857 1105 18-19 0.22924669677397702 1105 20-21 0.10397584432990925 1105 22-23 0.12333128902813684 1105 24-25 0.0802191859424326 1105 26-27 0.1064152903473925 1105 28-29 0.035882229656628795 1105 30-31 0.040344754230339674 1105 32-33 0.08712387249833142 1105 34-35 0.11408795165949925 1105 36-37 0.15653234868076282 1105 38-39 0.08629292745741424 1105 40-41 0.13753807308626875 1105 42-43 0.06627882419289932 1105 44-45 0.22480360042485614 1105 46-47 0.2225982946256977 1105 48-49 0.012928793591768795 1105 50-51 -0.06961156893194698 1105 52-53 -0.04872961699081202 1105 54-55 0.12616744862889107 1105 56-57 0.1992420736986631 1105 58-59 0.08442313316805894 1105 60-61 0.2078981592772422 1105 62-63 0.14138409303273392 1105 64-65 0.11583330049885632 1105 66-67 0.20690416876985296 1105 68-69 0.1088982065329418 1105 70-71 0.06800481772361167 1105 72-73 0.008623229852169345 1105 74-75 0.12501567968718774 1105 76-77 -0.011085877011026923 1105 78-79 0.08358836720254459 1105 80-81 0.08647834986670233 1105 82-83 -0.025383355778792804 1105 84-85 0.060631712210820865 1105 86-87 0.10988888731850466 1105 88-89 0.10152269133780578 1105 90-91 0.080809363144553 1105 92-93 0.06790170317930233 1105 94-95 -0.01772054605173423 1105 96-97 0.10779096221608953 1105 98-99 -0.12074118655111832 1105 100-101 -0.008719396637012267 1106 1 0.04237172375960796 1106 2 0.005601578406761121 1106 3 0.14163506003327342 1106 4 0.23234874492398916 1106 5 0.1694559100116777 1106 6 -0.04714839248889291 1106 7 0.07456914133772585 1106 8 0.08473583788732952 1106 9 0.16382914411237692 1106 10-11 0.19848999552932156 1106 12-13 0.17607417668335756 1106 14-15 0.1567353381000558 1106 16-17 0.16314753518670955 1106 18-19 0.16387009287462462 1106 20-21 -0.0015826042619977443 1106 22-23 0.08686132568869454 1106 24-25 0.0895374245693219 1106 26-27 0.0012901028786345137 1106 28-29 0.17103887021653463 1106 30-31 0.11065595236399162 1106 32-33 0.2266391616168093 1106 34-35 0.28666960471732494 1106 36-37 0.12203080727099547 1106 38-39 0.18214153602266236 1106 40-41 0.1149553785353632 1106 42-43 0.14498133210823738 1106 44-45 0.259045810056584 1106 46-47 0.2075945452505934 1106 48-49 0.19722620235698685 1106 50-51 -0.0750137935611761 1106 52-53 0.0011506113044745803 1106 54-55 -0.04643086831828924 1106 56-57 -0.07122326541148993 1106 58-59 0.0035428632130489746 1106 60-61 6.233300821136822E-4 1106 62-63 0.14773928383426949 1106 64-65 0.3886305426251653 1106 66-67 0.17000094430726165 1106 68-69 0.04614928968574361 1106 70-71 -0.0036467820097598747 1106 72-73 -0.006548199909538255 1106 74-75 0.16467469485132114 1106 76-77 0.1465107735472131 1106 78-79 0.0872169291088909 1106 80-81 0.08120318739378263 1106 82-83 0.13289234827053065 1106 84-85 0.10810921596019796 1106 86-87 0.17875241006471754 1106 88-89 0.16111275965975125 1106 90-91 0.12675587205973926 1106 92-93 0.09024172984152301 1106 94-95 0.03381461475479597 1106 96-97 0.17336649129457982 1106 98-99 0.23736058815309846 1106 100-101 0.10889016843547239 1107 1 0.048532649386544335 1107 2 -0.019077460212123754 1107 3 0.043223492881722336 1107 4 0.09710301462113335 1107 5 0.07202628859580784 1107 6 0.15198403132944094 1107 7 0.1124056555846451 1107 8 0.15367012068372077 1107 9 0.21937850617114663 1107 10-11 0.202439925752941 1107 12-13 0.16312647810934067 1107 14-15 0.17535149635805425 1107 16-17 0.13982918569167424 1107 18-19 0.2674292853399791 1107 20-21 0.12048947543237887 1107 22-23 0.14329558360049788 1107 24-25 0.14239029296570038 1107 26-27 0.15173508903286148 1107 28-29 0.18733628281689363 1107 30-31 0.10370767845835616 1107 32-33 0.1897878281016574 1107 34-35 0.17070146494956617 1107 36-37 0.09606125405879595 1107 38-39 0.17088275425250998 1107 40-41 0.13657361316315786 1107 42-43 0.2197338797920807 1107 44-45 0.17953831581487378 1107 46-47 0.46131155575587 1107 48-49 0.1887134345739554 1107 50-51 -0.009960510687143653 1107 52-53 0.19746809083441264 1107 54-55 0.20022339918901366 1107 56-57 0.05064600491274973 1107 58-59 0.003205428236469743 1107 60-61 -0.001225843451045705 1107 62-63 -0.07871790771134357 1107 64-65 0.14471258411673205 1107 66-67 -0.01636145687605506 1107 68-69 0.09850720368064003 1107 70-71 0.11511763860741553 1107 72-73 0.08986289543027937 1107 74-75 0.1691046487252592 1107 76-77 -0.04985597746161119 1107 78-79 -0.006128883014476116 1107 80-81 0.07586619038590925 1107 82-83 0.1451268546300355 1107 84-85 0.039054989462684375 1107 86-87 -0.04923020655938615 1107 88-89 -0.04264869471486321 1107 90-91 0.03384354941217538 1107 92-93 0.06585807125007648 1107 94-95 0.03591562029112083 1107 96-97 0.01166869567067863 1107 98-99 0.02724385954040187 1107 100-101 -0.019347316111197443 1108 1 0.11544098494334776 1108 2 0.05233621354333451 1108 3 0.09658575006978509 1108 4 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-1.3387427758928752 2316 92-93 -1.4464560285497505 2316 94-95 -1.3009257599554758 2316 96-97 -1.172478645627045 2316 98-99 -0.8314140920769653 2316 100-101 -1.2822237415567983 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 9.0 7 68.0 8 308.0 9 435.0 10 477.0 11 613.0 12 843.0 13 1123.0 14 1587.0 15 2494.0 16 3964.0 17 5436.0 18 6634.0 19 8405.0 20 10271.0 21 11597.0 22 10679.0 23 9434.0 24 8733.0 25 8762.0 26 9511.0 27 10929.0 28 12749.0 29 14746.0 30 17947.0 31 21219.0 32 27039.0 33 36014.0 34 49547.0 35 72776.0 36 115163.0 37 256255.0 38 576730.0 39 232916.0 40 113.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.293561578820764 26.293112976315086 15.008484438694241 21.40484100616991 2 12.74132477772077 24.367518980600494 43.648561760828194 19.24259448085054 3 14.262854593834776 33.39361017889839 32.816171398042435 19.52736382922439 4 9.128230272041343 20.599417082063795 47.0247707695321 23.24758187636277 5 8.694208099475068 43.0705947070836 38.735880928842214 9.499316264599113 6 21.812933631838664 46.97413234730616 20.854461792419496 10.35847222843568 7 22.15505778669846 34.732376304957654 29.555982140483884 13.556583767860003 8 21.251398719725742 39.98976973475217 24.3190520213526 14.439779524169488 9 25.88997854754775 16.91891180507248 26.065669528506348 31.125440118873428 10-11 20.26811351528518 29.72921189824641 34.00462752087879 15.998047065589613 12-13 20.763188484910174 29.760584208502895 31.63917059922993 17.837056707357 14-15 20.613283762474843 30.73022568832085 29.171842905160073 19.484647644044234 16-17 17.46728765267354 33.38017820696605 31.00187260763284 18.150661532727565 18-19 17.05259819278668 33.1444670274189 33.16459751961266 16.638337260181757 20-21 17.996823075728557 32.83163595424194 32.55444769398002 16.617093276049484 22-23 18.826782062040973 31.235882647148838 32.17507685080882 17.76225844000137 24-25 18.20065259293432 32.395972377059735 31.516563605812127 17.88681142419382 26-27 17.98328337195015 32.35313909106732 32.399401628053084 17.264175908929445 28-29 17.888655455388356 30.009860714177105 33.950296565508076 18.151187264926463 30-31 18.30048908883507 32.398075219649996 31.85922989375145 17.44220579776348 32-33 17.915248326299054 32.593671931968835 31.813226168161414 17.677853573570697 34-35 17.75394412391372 32.185979280853715 32.68228248358003 17.37779411165253 36-37 18.654381321063948 30.655983363603482 31.931858841676657 18.757776473655912 38-39 17.64365811791059 32.88690524332477 32.27358693830648 17.19584970045816 40-41 17.082555011979732 31.346103950303032 34.33479324528138 17.23654779243585 42-43 17.856142273800458 31.30647345533271 31.443578792217803 19.393805478649032 44-45 17.836245468838534 32.371825919166895 31.873653286491216 17.91827532550335 46-47 17.898013244603717 31.187906802132026 32.827061862939395 18.087018090324865 48-49 16.901974879302138 31.48544987679266 32.68274676277163 18.929828481133573 50-51 17.413105726693978 31.03476742134476 31.2337020124929 20.318424839468364 52-53 17.532454708130214 31.197508068957415 32.23804662754975 19.03199059536263 54-55 17.682788230837225 31.38984137468795 31.986459051745076 18.940911342729756 56-57 17.94221341940374 31.496779647566964 31.026253524411356 19.534753408617945 58-59 17.850556358477434 30.808376999294374 31.454776787897252 19.886289854330936 60-61 18.937332039596132 32.56799814686725 30.23224094613442 18.262428867402196 62-63 17.75380508174456 32.037341884632696 32.18811172210352 18.020741311519217 64-65 17.76943931794379 30.877342681219773 31.885700254073583 19.46751774676285 66-67 18.00402442899001 31.063727250851215 31.097507062830775 19.834741257328 68-69 17.672794358179427 31.870559939161407 30.861754796901327 19.594890905757836 70-71 18.296817522408166 30.394280089114556 32.66152012849075 18.64738225998653 72-73 18.553503102637972 30.810040075605556 30.882012988148265 19.75444383360821 74-75 17.77815792432211 30.908667956789 30.70572154028036 20.60745257860853 76-77 17.432789440851206 31.39815082608453 32.73055987507895 18.43849985798531 78-79 17.78994219525834 31.721040446472138 30.975753888261444 19.51326347000808 80-81 18.387507791440346 31.6400961686351 30.247788040442636 19.72460799948192 82-83 18.51720765763432 31.208551370395316 31.12063494119476 19.15360603077561 84-85 18.374960085341264 30.672917426367576 30.860297722872765 20.09182476541839 86-87 17.17931085391486 31.460501726944614 32.10650333539245 19.25368408374807 88-89 19.13485954000067 30.010741715973744 31.148838991166016 19.70555975285957 90-91 18.21382021336415 30.242243718143726 32.504994649053025 19.038941419439094 92-93 19.087170277116776 31.729065714005166 30.97137186950205 18.212392139376004 94-95 19.13635759894519 29.656279460025075 31.23587133642822 19.971491604601514 96-97 19.69151439535805 29.380998470621723 31.054603953198367 19.872883180821862 98-99 18.839545513266113 30.297065029497343 30.958889561977127 19.904499895259413 100-101 18.874699697731057 30.213718419499568 31.141308869523215 19.770273013246157 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 333.0 1 229.0 2 327.5 3 553.5 4 432.5 5 358.0 6 624.5 7 1200.0 8 1781.5 9 1942.5 10 1585.5 11 1241.0 12 1234.5 13 1311.0 14 1334.0 15 1345.0 16 1372.5 17 1383.5 18 1443.0 19 1551.5 20 1642.0 21 1806.0 22 2077.5 23 2299.5 24 2572.0 25 3140.5 26 4569.5 27 7323.0 28 9965.0 29 16931.0 30 28955.5 31 38880.5 32 61560.0 33 89851.0 34 110410.5 35 125971.0 36 149058.5 37 159576.5 38 135286.0 39 116669.5 40 95990.0 41 71826.0 42 57454.0 43 45936.5 44 39519.0 45 33866.5 46 26658.5 47 17480.0 48 12172.5 49 10097.0 50 8962.0 51 6658.5 52 4029.5 53 3018.5 54 2256.0 55 1778.0 56 1472.0 57 1447.0 58 1455.5 59 1272.0 60 1182.0 61 1226.0 62 1305.0 63 1485.0 64 1577.5 65 1396.0 66 1013.5 67 677.0 68 496.5 69 394.5 70 314.0 71 251.0 72 210.5 73 161.5 74 113.0 75 91.0 76 78.0 77 49.0 78 36.0 79 34.5 80 36.0 81 26.5 82 9.5 83 9.0 84 9.0 85 7.0 86 4.5 87 2.0 88 3.5 89 4.0 90 2.5 91 3.0 92 2.5 93 1.0 94 1.5 95 1.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47990103052227495 2 0.3750824152538261 3 0.206984413730721 4 0.23480663877111174 5 0.2584878814799095 6 0.2054962482053047 7 0.19695547214639408 8 0.19702017499532523 9 0.19708487784425638 10-11 0.2091519591699142 12-13 0.1982495291250169 14-15 0.2085372821050684 16-17 0.09210450545347962 18-19 0.039274629301202756 20-21 4.52919942517989E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.14147277918794043 46-47 0.20954017626350105 48-49 0.08941933722283726 50-51 0.06790563995323279 52-53 0.07651111886107456 54-55 0.08553716628696878 56-57 0.118373862119523 58-59 0.09792776185728233 60-61 0.14199040197938956 62-63 0.24412384901719608 64-65 0.13435546580551488 66-67 0.11035070885206145 68-69 0.11452404260812007 70-71 0.09362502240336144 72-73 0.07738460732164498 74-75 0.14639019570670714 76-77 0.22212488038060801 78-79 0.1441579474185828 80-81 0.08828703736654228 82-83 0.09362502240336144 84-85 0.10404218108127519 86-87 0.07641406458767785 88-89 0.10006295587201 90-91 0.12131784174588991 92-93 0.16961851847298687 94-95 0.1372994454318818 96-97 0.09288093964065332 98-99 0.07994036985442506 100-101 0.06738801716178365 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1545527.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.257570931390513 #Duplication Level Percentage of deduplicated Percentage of total 1 74.08008213303353 8.339617792158599 2 9.628928638054797 2.1679669427239863 3 3.836830946735818 1.2958018960389805 4 2.159915950455356 0.9726162807237168 5 1.3195012119742673 0.7427189243928031 6 0.9054934658579388 0.6116194151883828 7 0.6940133993836675 0.5469033549627963 8 0.5357038196692169 0.4824578998514433 9 0.4543674418236549 0.4603566334719822 >10 4.060859609795445 9.881133392801726 >50 0.8622798119785107 6.989567121082542 >100 1.1497064159713477 27.584592239697532 >500 0.19971704236203305 15.523609347190195 >1k 0.10845168769214258 21.578685634748474 >5k 0.004148425212267749 2.822353124966839 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 8611 0.5571562321460577 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 6300 0.40762794826619014 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 6179 0.399798903545522 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 5483 0.35476572068944767 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 5380 0.3481013272495401 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 5224 0.3380076828162821 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5127 0.33173150646996136 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4706 0.30449160706995093 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 4237 0.27414597092124565 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 3783 0.2447708775065075 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 3770 0.24392974047040264 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 3755 0.24295919773643554 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 3730 0.24134162651315702 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 3667 0.2372653470304951 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 3476 0.22490710288464713 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 3438 0.22244839462526375 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 3283 0.21241945304093685 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 3069 0.19857304336967257 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 3043 0.19689076929746294 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 3032 0.19617903795922037 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 3020 0.19540260377204666 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 3017 0.19520849522525327 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 2984 0.1930733012105256 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 2975 0.19249097557014533 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 2962 0.1916498385340405 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 2934 0.18983815876396853 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 2862 0.18517955364092636 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 2861 0.18511485079199522 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 2793 0.1807150570646776 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 2697 0.17450358356728807 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2695 0.17437417786942577 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 2643 0.1710096297250064 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 2633 0.170362601235695 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 2588 0.16745097303379364 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 2553 0.1651863733212037 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 2493 0.16130420238533524 No Hit CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG 2471 0.1598807397088501 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 2458 0.1590396026727453 No Hit CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT 2413 0.15612797447084392 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 2352 0.15218110068604432 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 2342 0.1515340721967329 No Hit CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC 2284 0.1477813069587267 No Hit CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG 2267 0.1466813585268973 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 2185 0.14137572491454373 No Hit TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA 2173 0.14059929072737 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 2166 0.140146370784852 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 2165 0.1400816679359209 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 2135 0.13814058246798666 No Hit GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG 2127 0.13762295967653754 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 2103 0.1360700913021901 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 2089 0.13516425141715413 No Hit ATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTA 2068 0.13380549158960017 No Hit ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT 2049 0.1325761374599085 No Hit CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA 2030 0.13134678333021682 No Hit ATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTT 2003 0.129599806409076 No Hit ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC 2001 0.1294704007112137 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 1990 0.12875866937297115 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 1958 0.12668817820717465 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1933 0.1250706069838961 No Hit CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT 1923 0.1244235784945847 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 1923 0.1244235784945847 No Hit GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC 1916 0.12397065855206671 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1913 0.12377655000527328 No Hit GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA 1907 0.12338833291168642 No Hit GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG 1875 0.12131784174588991 No Hit ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC 1851 0.11976497337154253 No Hit TTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGT 1837 0.11885913348650655 No Hit TATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGC 1821 0.11782388790360829 No Hit ACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTAT 1815 0.11743567081002144 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1751 0.1132946884784284 No Hit ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT 1743 0.11277706568697926 No Hit GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT 1726 0.11167711725514987 No Hit GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG 1702 0.11012424888080248 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1694 0.10960662608935334 No Hit ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA 1691 0.10941251754255993 No Hit CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG 1687 0.10915370614683535 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1679 0.10863608335538621 No Hit CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT 1663 0.10760083777248797 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 1656 0.10714791782996996 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 1655 0.10708321498103883 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1653 0.10695380928317656 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 1650 0.10675970073638313 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 1648 0.10663029503852084 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1607 0.10397747823234404 No Hit ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT 1605 0.10384807253448176 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 1604 0.10378336968555062 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 1590 0.10287752980051464 No Hit GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 1589 0.10281282695158352 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 1578 0.10210109561334096 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 1576 0.10197168991547866 No Hit ACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGT 1576 0.10197168991547866 No Hit ATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATG 1575 0.10190698706654754 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 1574 0.10184228421761639 No Hit GTAAAAGGCCTAGGAGATTTGTTGATCCAATAAATATGATTAGGGAAACA 1552 0.10041882154113127 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 6.470284893114129E-5 0.0 4 0.0 0.0 0.0 1.2940569786228258E-4 0.0 5 0.0 0.0 0.0 1.2940569786228258E-4 0.0 6 0.0 0.0 0.0 1.2940569786228258E-4 0.0 7 0.0 0.0 0.0 1.9410854679342387E-4 0.0 8 0.0 0.0 0.0 1.9410854679342387E-4 0.0 9 0.0 0.0 0.0 1.9410854679342387E-4 0.0 10-11 0.0 0.0 0.0 0.0011646512807605433 0.0 12-13 0.0 0.0 0.0 0.00449684800071432 0.0 14-15 0.0 0.0 0.0 0.008896641728031927 0.0 16-17 0.0 0.0 0.0 0.026431113788371215 0.0 18-19 0.0 0.0 0.0 0.04470966861141863 0.0 20-21 0.0 0.0 0.0 0.08052269549480534 0.0 22-23 0.0 0.0 0.0 0.15997779398224682 0.0 24-25 0.0 0.0 0.0 0.2379447269442721 0.0 26-27 0.0 0.0 0.0 0.26958442007160016 0.0 28-29 0.0 0.0 0.0 0.294009745543106 0.0 30-31 0.0 0.0 0.0 0.32674938710226353 0.0 32-33 0.0 0.0 0.0 0.3688062389075053 0.0 34-35 0.0 0.0 0.0 0.4225743063692837 0.0 36-37 0.0 0.0 0.0 0.4908681634161034 0.0 38-39 0.0 0.0 0.0 0.5928722047560475 0.0 40-41 0.0 0.0 0.0 0.7137047751349539 0.0 42-43 0.0 0.0 0.0 0.821532072878701 0.0 44-45 0.0 0.0 0.0 0.9379001466813586 0.0 46-47 0.0 0.0 0.0 1.0860373193092063 0.0 48-49 0.0 0.0 0.0 1.236924363016628 0.0 50-51 0.0 0.0 0.0 1.401269599301727 0.0 52-53 0.0 0.0 0.0 1.5697558179184188 0.0 54-55 0.0 0.0 0.0 1.7341010542035176 0.0 56-57 0.0 0.0 0.0 1.921092287614516 0.0 58-59 0.0 0.0 0.0 2.133511740655453 0.0 60-61 0.0 0.0 0.0 2.3635950714545912 0.0 62-63 0.0 0.0 0.0 2.584393543432111 0.0 64-65 0.0 0.0 0.0 2.800404004588726 0.0 66-67 0.0 0.0 0.0 3.047633590354617 0.0 68-69 0.0 0.0 0.0 3.3303526887592385 0.0 70-71 0.0 0.0 0.0 3.6735689509144773 0.0 72-73 0.0 0.0 0.0 4.0025182348804 0.0 74-75 0.0 0.0 0.0 4.319206328973872 0.0 76-77 0.0 0.0 0.0 4.672548586986833 0.0 78-79 0.0 0.0 0.0 5.095640516147567 0.0 80-81 0.0 0.0 0.0 5.576317980857015 0.0 82-83 0.0 0.0 0.0 6.086273484707805 0.0 84-85 0.0 0.0 0.0 6.545178440751925 0.0 86-87 0.0 0.0 0.0 7.010521330264693 0.0 88-89 0.0 0.0 0.0 7.516497608906217 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGACGG 25 5.2508352E-5 76.07307 4 GTACACG 30 1.2809088E-4 63.53647 1 ATGTATT 145 0.0 59.154648 1 ACGGAGA 25 0.005014755 57.01965 7 GACGGGA 35 2.7821635E-4 54.309715 6 GTATGGT 160 0.0 53.6089 1 GGTCAAC 120 0.0 47.51946 8 GCCATGC 50 2.8206136E-5 47.51946 9 GACATAT 560 0.0 45.09955 1 GGGATGC 315 0.0 43.745235 7 GGTATGC 165 0.0 43.199505 8 CGGGACC 45 9.606718E-4 42.239517 8 AAAGACA 175 0.0 40.75343 4 GGATGCC 330 0.0 40.31954 8 ACATATA 635 0.0 39.731483 2 GTCAACA 145 0.0 39.326447 9 ACACGGG 50 0.0016086327 38.035297 3 GGAACTT 50 0.0016127396 38.015564 8 TGGGTAT 640 0.0 37.868298 6 GGATGAC 720 0.0 37.61957 8 >>END_MODULE