FastQCFastQC Report
Fri 10 Feb 2017
SRR2031648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545527
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT96760.6260647662577231No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC69530.4498789086182254No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC64200.41539229013792706No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT60610.3921639673716473No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA58290.37715290641962257No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC58100.37592355228993085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56230.3638241195398074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56210.3636947138419452No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA49860.3226084047706705No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC43570.28191031279298256No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT41130.2661228176537841No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC40140.25971723560960114No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA39870.2579702586884603No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC39800.2575173387459423No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC37210.24075930087277672No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT35500.22969511370555157No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT34810.22523061712930284No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC34780.22503650858250937No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG34620.22400126299961112No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG34610.22393656015067998No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT33390.21604281258108077No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC33090.2141017271131465No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC32840.21248415588986802No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT32400.20963723053689776No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA32060.207437333673239No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC31920.20653149378820299No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA31380.20303753994592136No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT31270.2023258086076788No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC30450.19702017499532523No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA30120.19488498098059756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29810.19287919266373219No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT28030.18136208555398903No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT27100.17534472060339287No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA26410.17088022402714415No Hit
CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT26030.16842151576776077No Hit
ATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTA25010.16182182517678437No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT24960.16149831093212866No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC24750.16013955110457467No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA23970.15509272888794567No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT23540.15231050638390659No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG23340.15101644940528378No Hit
GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG23010.1488812553905561No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT22990.1487518496926938No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT22970.14862244399483154No Hit
TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA22930.14836363259910695No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA22610.14629314143331046No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG21910.14176394200813056No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC21670.14021107363378316No Hit
CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC21650.1400816679359209No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA21640.14001696508698974No Hit
ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA21620.13988755938912747No Hit
GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG21420.13859350241050464No Hit
ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT21000.1358759827553967No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG20950.135552468510741No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG20890.13516425141715413No Hit
ATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTT20740.13419370868318703No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG20590.1332231659492199No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC20390.1319291089705971No Hit
ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC20160.13044094344518084No Hit
TTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGT20150.1303762405962497No Hit
GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG20120.13018213204945625No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA19880.12862926367510888No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT19810.1281763437325909No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA19700.12746461239434834No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC19440.12578233832213867No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC19220.12435887564565355No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA19060.1233236300627553No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA19050.12325892721382414No Hit
TATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGC18910.12235308732878816No Hit
ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC18890.1222236816309259No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA18540.11995908191833596No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA18340.11866502493971312No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT18330.11860032209078197No Hit
ACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTAT18280.11827680784612628No Hit
ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT18150.11743567081002144No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA17940.11607691098246746No Hit
GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT17660.11426523121239553No Hit
CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG17620.11400641981667095No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA17510.1132946884784284No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT17330.11213003719766784No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC17090.11057716882332046No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG17090.11057716882332046No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC16970.10980073463614677No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT16950.10967132893828448No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA16910.10941251754255993No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC16860.10908900329790422No Hit
ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT16850.10902430044897307No Hit
ATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATG16780.10857138050645507No Hit
ACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGGTAAAA16720.10818316341286824No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA16640.10766554062141911No Hit
TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA16570.10721262067890111No Hit
CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT16370.10591856370027827No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA16310.10553034660669143No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC16150.10449510102379317No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT16150.10449510102379317No Hit
GCCATGAAGTCATTCTAAATTTGTTGAAGCATACGATACTGATATTACTT16120.10430099247699974No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC15950.10320104404517036No Hit
TAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTC15870.10268342125372121No Hit
GGCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTG15610.10100114718151156No Hit
GTAAAAGGCCTAGGAGATTTGTTGATCCAATAAATATGATTAGGGAAACA15580.10080703863471813No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAG200.002071411671.274281
ATGTATT1750.057.0194241
GACATAT7800.056.6539151
GTTATAT902.2555469E-1047.5161821
ACATATA9300.047.5038832
ATACGAC405.389167E-447.499273
GGATGAC5100.047.4946638
GATGACC3550.046.8257269
CATATAG9700.046.51993
GTATGGT1700.044.721111
AGGGTCT1700.041.907055
CATGGGT6600.041.7417834
GGTATCA39350.041.0559121
ATGGGAC2550.040.975795
GGGGAGC3150.039.2019467
TGGGGAG3800.037.4957856
TGGGTAT4450.037.355356
GTCAACA1302.055458E-1036.534359
TAACTTT16300.035.839536
GATCCTC801.0786782E-535.6209955
Produced by FastQC (version 0.11.5)