##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031648_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1545527 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51370244583239 34.0 31.0 34.0 31.0 34.0 2 32.71206002871512 34.0 31.0 34.0 31.0 34.0 3 32.80227715206528 34.0 31.0 34.0 31.0 34.0 4 36.25717182553265 37.0 37.0 37.0 35.0 37.0 5 36.021879268366064 37.0 35.0 37.0 35.0 37.0 6 36.088261803255456 37.0 37.0 37.0 35.0 37.0 7 36.03438762312143 37.0 37.0 37.0 35.0 37.0 8 36.141712179728984 37.0 37.0 37.0 35.0 37.0 9 38.00847219103905 39.0 39.0 39.0 35.0 39.0 10-11 37.97486391373299 39.0 38.5 39.0 35.0 39.0 12-13 38.015014943122964 39.0 39.0 39.0 35.0 39.0 14-15 39.43938345949311 41.0 39.0 41.0 36.5 41.0 16-17 39.39699532910133 41.0 39.0 41.0 36.0 41.0 18-19 39.48460039844014 41.0 39.5 41.0 37.0 41.0 20-21 39.52637546933829 41.0 40.0 41.0 37.0 41.0 22-23 39.42573245242561 41.0 39.0 41.0 36.0 41.0 24-25 39.366575931704844 41.0 39.0 41.0 36.0 41.0 26-27 39.25626760321883 41.0 39.0 41.0 36.0 41.0 28-29 39.1827900774299 41.0 39.0 41.0 36.0 41.0 30-31 39.01235468548916 40.0 39.0 41.0 35.0 41.0 32-33 38.8336632100248 40.0 38.0 41.0 35.0 41.0 34-35 38.73312565875588 40.0 38.0 41.0 35.0 41.0 36-37 38.57238922386992 40.0 38.0 41.0 35.0 41.0 38-39 38.43055378521372 40.0 38.0 41.0 34.5 41.0 40-41 38.343894348012036 40.0 38.0 41.0 34.0 41.0 42-43 38.2050730915733 40.0 38.0 41.0 34.0 41.0 44-45 38.105067721236836 40.0 38.0 41.0 33.5 41.0 46-47 38.07135106665882 40.0 38.0 41.0 33.5 41.0 48-49 38.152672195309435 40.0 38.0 41.0 34.0 41.0 50-51 38.10488752380256 40.0 38.0 41.0 33.0 41.0 52-53 38.075427669655724 40.5 38.0 41.0 33.0 41.0 54-55 38.01214601880135 40.0 38.0 41.0 33.0 41.0 56-57 37.93512989420437 40.0 38.0 41.0 33.0 41.0 58-59 37.79264030974548 40.0 38.0 41.0 33.0 41.0 60-61 37.59794976082591 40.0 38.0 41.0 33.0 41.0 62-63 37.43259354252627 40.0 37.5 41.0 32.0 41.0 64-65 37.208775712103375 40.0 37.0 41.0 31.5 41.0 66-67 36.985534384064465 39.5 37.0 41.0 31.0 41.0 68-69 36.704542204697816 39.0 36.0 41.0 31.0 41.0 70-71 36.35476151500427 39.0 36.0 40.5 31.0 41.0 72-73 35.9896271627736 38.0 35.5 40.0 30.5 41.0 74-75 35.56941580444729 37.0 35.0 39.5 30.0 41.0 76-77 34.884249838404635 36.5 34.5 39.0 29.0 40.0 78-79 34.770958708582896 36.0 35.0 39.0 29.0 40.0 80-81 34.40814912971433 36.0 35.0 37.5 29.0 39.0 82-83 34.037630853424105 35.5 34.5 37.0 29.0 39.0 84-85 33.63226265215684 35.0 34.0 37.0 29.0 38.5 86-87 33.34613112549958 35.0 34.0 36.0 29.0 37.0 88-89 33.053130420885566 35.0 34.0 36.0 28.5 37.0 90-91 32.8574893871152 35.0 34.0 36.0 27.5 36.5 92-93 32.70101719348804 35.0 34.0 35.0 27.0 36.0 94-95 32.51772955114987 35.0 34.0 35.0 26.5 36.0 96-97 32.3761435419763 35.0 34.0 35.0 26.0 36.0 98-99 32.28215844821864 35.0 34.0 35.0 26.0 36.0 100-101 31.885857704200575 34.5 33.0 35.0 25.0 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09183222870512253 1101 2 0.09601883909672893 1101 3 0.13053720755119969 1101 4 0.1479216104084884 1101 5 0.193465213758806 1101 6 0.16563848919167157 1101 7 0.25829397145331257 1101 8 0.17606250875950735 1101 9 0.21629522431624082 1101 10-11 0.15251707226759237 1101 12-13 0.12562980101206023 1101 14-15 0.09993630591679192 1101 16-17 0.17587308941255486 1101 18-19 0.0842103232572029 1101 20-21 0.014774110829186782 1101 22-23 0.15885347199760247 1101 24-25 0.11256762613576399 1101 26-27 0.23006864355538426 1101 28-29 0.15349382725201366 1101 30-31 -0.003589933790294708 1101 32-33 0.029345053335305238 1101 34-35 0.04449784544452484 1101 36-37 0.08403425585073165 1101 38-39 0.05707060949173837 1101 40-41 0.06919937639474938 1101 42-43 -0.10133806670619805 1101 44-45 -0.1977590987421607 1101 46-47 -0.14060914109250433 1101 48-49 -0.08694208098857104 1101 50-51 -0.014817966625180645 1101 52-53 -0.1700984299520698 1101 54-55 -0.03089232518083662 1101 56-57 0.06406952106709696 1101 58-59 -0.1103811490124329 1101 60-61 -0.11207212249407661 1101 62-63 -0.1969167856669003 1101 64-65 -0.18297027499078666 1101 66-67 -0.24920431519492325 1101 68-69 -0.23899448067581375 1101 70-71 -0.17976291661760513 1101 72-73 -0.16341174699932992 1101 74-75 -0.3341339454991967 1101 76-77 -0.31449963780826806 1101 78-79 -0.2663606128695548 1101 80-81 -0.17094252924906073 1101 82-83 -0.12239083441478016 1101 84-85 -0.20622584465047566 1101 86-87 -0.11487590366321143 1101 88-89 -0.054328791208028804 1101 90-91 -0.03425707125813915 1101 92-93 -0.29880286616320717 1101 94-95 -0.10669767998806634 1101 96-97 -0.2175165619724524 1101 98-99 -0.36152053183335653 1101 100-101 -0.2394596203751469 1102 1 0.10432202906415 1102 2 0.12876512642741744 1102 3 0.0387145997069922 1102 4 0.15283919730943296 1102 5 -0.0230106062923241 1102 6 0.020970861492166648 1102 7 0.15522999930512782 1102 8 0.08901469284262475 1102 9 0.15520617465079312 1102 10-11 0.11590308108007719 1102 12-13 0.03591783887312516 1102 14-15 0.11101855582159459 1102 16-17 0.15971685811189928 1102 18-19 0.2657455732164067 1102 20-21 0.1747201207296385 1102 22-23 0.06679899231528452 1102 24-25 0.06245611006112739 1102 26-27 0.08037871264095742 1102 28-29 -0.1667313808206643 1102 30-31 -0.227970475595221 1102 32-33 0.09511505823112998 1102 34-35 0.19317325207020986 1102 36-37 0.1227670524562896 1102 38-39 0.09680501084081072 1102 40-41 0.06749943623264443 1102 42-43 0.10552629166839012 1102 44-45 0.10497712494277067 1102 46-47 0.3338081194199276 1102 48-49 0.2572407237766967 1102 50-51 0.3194834259657995 1102 52-53 0.11210931633525689 1102 54-55 0.08446561421969534 1102 56-57 0.15006541401166373 1102 58-59 0.19156494248514377 1102 60-61 0.49760597683682306 1102 62-63 0.30823267307215474 1102 64-65 0.534382714183991 1102 66-67 0.5182057919693079 1102 68-69 0.26859337223176283 1102 70-71 0.4346824949039103 1102 72-73 0.47831294429153104 1102 74-75 0.49828731327249187 1102 76-77 0.42306563989395585 1102 78-79 0.34741978206054114 1102 80-81 0.25317758172302973 1102 82-83 -0.08127540167801328 1102 84-85 0.26688926169778426 1102 86-87 0.32216443251694926 1102 88-89 0.3807321346473813 1102 90-91 0.34649076641797194 1102 92-93 0.32959361428067524 1102 94-95 0.3727058450078573 1102 96-97 0.23444924883100526 1102 98-99 0.2793752686344746 1102 100-101 0.17414602070629215 1103 1 0.23521686577642953 1103 2 0.21822632686684784 1103 3 0.15546033025727723 1103 4 0.14563356463191468 1103 5 0.0755088184555035 1103 6 0.17086849456916298 1103 7 0.22809777120166075 1103 8 0.17434875855720122 1103 9 0.17992404142398755 1103 10-11 0.14944198051081514 1103 12-13 0.13248480182129896 1103 14-15 0.3086516328215012 1103 16-17 0.21548186360475796 1103 18-19 0.26991140763166754 1103 20-21 0.1396494625852185 1103 22-23 0.12166391791200937 1103 24-25 0.25983202137641825 1103 26-27 0.21521592426584135 1103 28-29 0.2700458088327906 1103 30-31 0.2321975559637508 1103 32-33 0.251019881960282 1103 34-35 0.21969127863567195 1103 36-37 0.20626850057374213 1103 38-39 0.15891801393678406 1103 40-41 0.186195131828363 1103 42-43 0.18408291805408794 1103 44-45 0.12665394507659045 1103 46-47 0.1430223908670456 1103 48-49 -0.002370531591317615 1103 50-51 0.1673224851888122 1103 52-53 0.1381429725821377 1103 54-55 0.10465187208446736 1103 56-57 0.0627551673323481 1103 58-59 0.19746477270585672 1103 60-61 0.14488076960622465 1103 62-63 0.06762920757729773 1103 64-65 0.03037792041588716 1103 66-67 0.1015923893926498 1103 68-69 0.14825106722826575 1103 70-71 0.161377791018964 1103 72-73 0.09256941088496262 1103 74-75 0.18711433814016232 1103 76-77 0.07472722982704738 1103 78-79 0.21215812221833374 1103 80-81 0.2550027090575 1103 82-83 0.1460590635172352 1103 84-85 0.052864993246764413 1103 86-87 0.06770897461223058 1103 88-89 0.12793826667574848 1103 90-91 0.19867917149137782 1103 92-93 0.054345187235831816 1103 94-95 0.27046375971873715 1103 96-97 0.2037279365369855 1103 98-99 0.03653221460471201 1103 100-101 0.22538565917627906 1104 1 0.04874571120419802 1104 2 0.07168900526297506 1104 3 0.13358084675655846 1104 4 0.1148130368058915 1104 5 0.06541386262978222 1104 6 -0.10613869790988417 1104 7 0.06708582149238396 1104 8 0.016109500081022077 1104 9 0.18116998267596784 1104 10-11 0.049796177067491953 1104 12-13 0.14006073816502607 1104 14-15 0.20351708060523066 1104 16-17 0.2538110752637479 1104 18-19 0.22349537652089424 1104 20-21 0.16119976274379866 1104 22-23 0.28879938570631225 1104 24-25 0.27593958763784343 1104 26-27 0.16787133655912356 1104 28-29 0.16961303302670672 1104 30-31 0.21525582259517506 1104 32-33 0.12916305193687805 1104 34-35 0.36690702469019243 1104 36-37 0.26409032153575396 1104 38-39 0.16349708401153862 1104 40-41 0.12820458965514803 1104 42-43 0.15541565044100736 1104 44-45 0.16625269535976628 1104 46-47 0.22789741918388984 1104 48-49 0.1690301612275249 1104 50-51 0.2094544133772871 1104 52-53 0.16795117314092067 1104 54-55 0.2193126834565149 1104 56-57 0.17038160205257924 1104 58-59 0.2650995608958411 1104 60-61 0.3918673851767167 1104 62-63 0.2859839515930034 1104 64-65 0.24846365438855145 1104 66-67 0.3416543560920431 1104 68-69 0.35726125185411206 1104 70-71 0.4200557246442713 1104 72-73 0.376773801883985 1104 74-75 0.08124118817413972 1104 76-77 0.23518700102692236 1104 78-79 0.16049757513686558 1104 80-81 0.23209673986622903 1104 82-83 0.3159684024132545 1104 84-85 0.31819974556874797 1104 86-87 0.22735227673410208 1104 88-89 0.22281268146091548 1104 90-91 0.13454151386092406 1104 92-93 0.24069953481568973 1104 94-95 0.34191070338709295 1104 96-97 0.2108531630717536 1104 98-99 0.30437772732840784 1104 100-101 0.10329875477395589 1105 1 0.1350620670148146 1105 2 0.06787802965322243 1105 3 0.06060738908080765 1105 4 0.08777752748021328 1105 5 0.04710324570008595 1105 6 0.16169666794377946 1105 7 0.21350798791993242 1105 8 0.0872357554612222 1105 9 0.17856955225990134 1105 10-11 0.14650080404022958 1105 12-13 0.0864515200875502 1105 14-15 0.11697206625803602 1105 16-17 0.14752520725800622 1105 18-19 0.10443153147068074 1105 20-21 0.12651091769357947 1105 22-23 0.08391236992439133 1105 24-25 0.15215013283152956 1105 26-27 0.1689697942348758 1105 28-29 0.11694983360059297 1105 30-31 0.1906727881618977 1105 32-33 0.2230206559540946 1105 34-35 0.2641765727533141 1105 36-37 0.25195348509386406 1105 38-39 0.24165795201727747 1105 40-41 0.24892732997077616 1105 42-43 0.27078957440083684 1105 44-45 0.17543490482891144 1105 46-47 0.3931771896286591 1105 48-49 0.280117858501562 1105 50-51 0.3874269743528984 1105 52-53 0.4788550468855419 1105 54-55 0.3642687451494879 1105 56-57 0.3748875202735249 1105 58-59 0.3692333485572519 1105 60-61 0.3240409863244835 1105 62-63 0.47037688559586854 1105 64-65 0.32650795137563904 1105 66-67 0.3812750519313539 1105 68-69 0.29828976691867126 1105 70-71 0.3817531780158703 1105 72-73 0.4764509898323297 1105 74-75 0.42749746507514175 1105 76-77 0.3049323381861839 1105 78-79 0.34712329249697405 1105 80-81 0.47810642422778216 1105 82-83 0.42299404803735996 1105 84-85 0.2757365102746263 1105 86-87 0.3362654761602144 1105 88-89 0.4347881118769834 1105 90-91 0.3526458897576248 1105 92-93 0.4387492120036356 1105 94-95 0.32429502906857977 1105 96-97 0.1832751501592753 1105 98-99 0.2998449081031609 1105 100-101 0.2749406127366463 1106 1 0.191377681079139 1106 2 0.12506641492234394 1106 3 0.11280077351673157 1106 4 0.08358031034973123 1106 5 0.11908708172741456 1106 6 0.05996154046502511 1106 7 0.20438242551366415 1106 8 0.10164377079200193 1106 9 0.16208271673248476 1106 10-11 0.15304554658314373 1106 12-13 0.14674667922769657 1106 14-15 0.00828393094725044 1106 16-17 0.09878749687640465 1106 18-19 0.004410701608982492 1106 20-21 -0.028803821516547146 1106 22-23 0.08603389133749317 1106 24-25 -0.015806249438092834 1106 26-27 0.059269327645978365 1106 28-29 0.0702534496645768 1106 30-31 0.06373769401091778 1106 32-33 0.08299086925187993 1106 34-35 -0.08917391049946843 1106 36-37 0.07395502650363284 1106 38-39 0.10512654058585014 1106 40-41 0.061902250817304605 1106 42-43 0.08410506348598545 1106 44-45 0.153722898496639 1106 46-47 0.225271840520179 1106 48-49 0.1748718578350008 1106 50-51 0.19470388653420656 1106 52-53 0.0259971898617124 1106 54-55 0.08773254284987075 1106 56-57 -0.018522952980937646 1106 58-59 0.13544002244493214 1106 60-61 0.07071549890572015 1106 62-63 0.07321599574418514 1106 64-65 0.19275107752128662 1106 66-67 0.185351455864037 1106 68-69 0.12759113335760475 1106 70-71 0.06846893372324558 1106 72-73 0.10372873520810089 1106 74-75 0.173351281105802 1106 76-77 0.10598737068075792 1106 78-79 0.158566177016219 1106 80-81 0.18785271651240265 1106 82-83 0.35358478291487216 1106 84-85 0.23325817333078902 1106 86-87 0.18081950882143616 1106 88-89 0.29001432417493334 1106 90-91 0.26320350515506163 1106 92-93 0.13046517600964336 1106 94-95 0.14010281110490297 1106 96-97 -0.06391386000569099 1106 98-99 0.2118012487130514 1106 100-101 0.28836858307492363 1107 1 0.09641959667460753 1107 2 0.03823115684928524 1107 3 0.11464969820403326 1107 4 0.11593624353163534 1107 5 0.09469222507257768 1107 6 0.058718804275471825 1107 7 0.1245332659158791 1107 8 0.053972868437121235 1107 9 0.051608595705808114 1107 10-11 0.06919519451602696 1107 12-13 0.0229834431167788 1107 14-15 0.017095562599642733 1107 16-17 0.13709880901072324 1107 18-19 -0.024980233235204707 1107 20-21 0.058707550738922976 1107 22-23 0.09161848996903643 1107 24-25 0.12521992118256975 1107 26-27 0.09064779431755454 1107 28-29 -0.05284885186094357 1107 30-31 0.03541928479287293 1107 32-33 0.033476765664310904 1107 34-35 0.034844641456558634 1107 36-37 0.06065894373551117 1107 38-39 -0.05599716765532037 1107 40-41 -0.02047916417993889 1107 42-43 -0.05177796104623411 1107 44-45 -0.11020774119646859 1107 46-47 0.046804571712897314 1107 48-49 -0.09212879314575417 1107 50-51 -0.02576258994787395 1107 52-53 -0.29407083464064243 1107 54-55 -0.16560522657552923 1107 56-57 -0.16628662506978742 1107 58-59 -0.10381680439218854 1107 60-61 -0.007575885652691738 1107 62-63 -0.13644434957423357 1107 64-65 -0.2954712971052018 1107 66-67 -0.13813247566154985 1107 68-69 -0.1953540468963837 1107 70-71 -0.12836241249300429 1107 72-73 -0.025973207183092484 1107 74-75 -0.1071101885977086 1107 76-77 -0.2002888701144272 1107 78-79 -0.007676788111517396 1107 80-81 -0.08461059863196141 1107 82-83 -0.07897052556439377 1107 84-85 -0.023870264972359223 1107 86-87 -0.15770516374718824 1107 88-89 -0.18257630050779028 1107 90-91 -0.12233163287475435 1107 92-93 0.03182566605679682 1107 94-95 -0.023291366698437344 1107 96-97 -0.15965481346356114 1107 98-99 -0.0833854254919757 1107 100-101 -0.05609081424032425 1108 1 0.09642570337845058 1108 2 0.112998350508839 1108 3 0.023775838278666583 1108 4 0.06404758095614937 1108 5 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-1.147891190568977 2316 80-81 -1.3407159911019946 2316 82-83 -1.6168371685401866 2316 84-85 -1.3605407847342832 2316 86-87 -1.1065180577078806 2316 88-89 -0.9715008809928918 2316 90-91 -0.7331829872477229 2316 92-93 -0.6594933236469913 2316 94-95 -0.7483459023039671 2316 96-97 -0.7175409149031111 2316 98-99 -0.7849896507203695 2316 100-101 -1.0278334514344714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 2.0 12 9.0 13 38.0 14 200.0 15 748.0 16 1857.0 17 3859.0 18 5475.0 19 6578.0 20 8430.0 21 10467.0 22 10279.0 23 8100.0 24 7014.0 25 6959.0 26 8218.0 27 9671.0 28 11991.0 29 14250.0 30 17238.0 31 21038.0 32 26490.0 33 33661.0 34 45846.0 35 68079.0 36 113313.0 37 251791.0 38 602786.0 39 250970.0 40 169.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.306301465985605 27.459055391819557 15.472695718819729 20.761947423375112 2 12.62831582986251 25.184357455145673 43.65484893914797 18.53247777584385 3 14.027170108222336 34.16327946156703 33.22922611260144 18.580324317609197 4 8.944793986114535 21.390850140129434 47.28143723424426 22.382918639511775 5 8.313669059162345 43.42149959204854 39.293199018845996 8.97163232994312 6 21.48503390752798 47.79088298036851 20.831017510532007 9.893065601571504 7 21.712982044312394 35.148528624863886 29.88449894437302 13.253990386450706 8 20.854634050391873 40.572309639365734 24.497210336668335 14.075845973574063 9 25.62511039923599 16.889578765042604 26.356576106402542 31.128734729318868 10-11 20.00081525589653 29.938655228928386 34.47212504213773 15.58840447303735 12-13 20.527399391922625 29.953633938455944 31.907757030449808 17.61120963917162 14-15 20.546033812414795 30.781151024860776 29.44451957164126 19.22829559108317 16-17 17.384102639423315 33.692261603970685 31.12951116350604 17.79412459309996 18-19 16.97210724885427 33.124073536081866 33.41750095598459 16.486318259079265 20-21 17.885226204395003 32.75313210315963 32.966845613179196 16.394796079266165 22-23 18.69661697056636 31.279038054834913 32.43798825423724 17.58635672036149 24-25 18.2298012263778 32.21160160903045 31.76188445753455 17.796712707057203 26-27 18.057044619731652 31.82917542042294 32.88923454588629 17.22454541395912 28-29 17.949684553662117 29.74916054094266 34.27487790348316 18.02627700191206 30-31 18.26864118499574 32.25873149050852 32.064265142085006 17.40836218241073 32-33 18.010464395143014 32.28776730777505 32.042316970948086 17.659451326133848 34-35 17.690341223414407 31.940593726282362 33.25063230859118 17.11843274171205 36-37 18.79116314370438 30.396460236540673 32.21674548552047 18.595631134234473 38-39 17.4396500352307 32.750964557720444 32.70324620663372 17.106139200415132 40-41 17.020504979854767 31.06500242312169 34.72918946094116 17.185303136082386 42-43 17.892667032022086 31.145686875738825 31.56117622015015 19.400469872088937 44-45 17.890855352252014 32.134799327349185 32.12234402892994 17.852001291468863 46-47 17.784807382853874 31.088392503010297 33.01294639304263 18.113853721093193 48-49 16.727963188428873 31.43721896321497 32.9400841063362 18.894733742019962 50-51 17.363900445279654 30.859316608005717 31.2640221675626 20.512760779152035 52-53 17.47895701595637 30.899136669886712 32.4500316073417 19.171874706815213 54-55 17.700005143911437 31.12483755430628 32.06348816075403 19.11166914102826 56-57 18.090043072686534 31.268557585859064 30.990141226908364 19.651258114546042 58-59 17.764911256807547 30.711336650864073 31.335427980229397 20.18832411209898 60-61 19.20407679974326 32.537498560360675 29.951744584044526 18.306680055851533 62-63 17.618618115374236 32.055603040257466 32.27598094371693 18.049797900651363 64-65 17.581187517267573 30.79046823510686 31.80031794979965 19.82802629782592 66-67 17.82159095247123 31.082278083786306 31.091789402579185 20.00434156116328 68-69 17.5950662783633 31.893295943713696 30.670638558886388 19.840999219036615 70-71 18.173736207778965 30.18481721768691 32.75128807196509 18.890158502569026 72-73 18.54626286050001 30.705028123093285 30.69926956953842 20.049439446868288 74-75 17.67112447728186 30.99651445752808 30.445407941757086 20.886953123432978 76-77 17.154763391386886 31.733512258278246 32.44084380279348 18.670880547541387 78-79 17.48629672321597 32.0132097550055 30.72274169707436 19.777751824704172 80-81 18.10323071606894 31.770487538777527 30.10067596162433 20.02560578352921 82-83 18.14839451084734 31.471003877192892 30.955069339117202 19.425532272842567 84-85 18.022978569769403 30.946013883937322 30.552523508162587 20.478484038130684 86-87 16.55173283934865 31.96932179120779 31.7958534532234 19.683091916220164 88-89 18.64237894258722 30.34922715682094 30.846500902281228 20.16189299831061 90-91 17.687656055183766 30.620170336720097 32.24637939033094 19.445794217765204 92-93 18.5534125253069 32.10209203721449 30.523180766172313 18.82131467130629 94-95 18.46425199947979 30.119790854511113 30.790856452200448 20.625100693808648 96-97 19.1143862255399 29.886504732689883 30.66957096187902 20.329538079891197 98-99 18.127958942160184 30.644628013616064 30.680149877679263 20.547263166544486 100-101 17.781086969040334 30.678014683664536 30.961704324803126 20.579194022492004 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 344.0 1 251.0 2 360.5 3 574.5 4 419.5 5 310.5 6 564.0 7 1128.5 8 1770.0 9 2012.0 10 1614.5 11 1151.0 12 1097.5 13 1150.0 14 1176.5 15 1185.0 16 1177.5 17 1204.0 18 1220.0 19 1255.0 20 1347.5 21 1536.0 22 1817.5 23 2019.0 24 2227.0 25 2786.5 26 4375.5 27 7085.0 28 9598.5 29 17087.5 30 29829.5 31 39638.5 32 63869.0 33 94437.5 34 115003.0 35 129377.5 36 151115.0 37 161259.0 38 135956.5 39 115450.0 40 94081.5 41 69958.5 42 56264.5 43 44422.5 44 37652.0 45 32830.5 46 25848.5 47 16789.0 48 11576.0 49 9601.5 50 8622.5 51 6404.0 52 3540.5 53 2388.0 54 1746.5 55 1386.0 56 1262.5 57 1312.0 58 1331.0 59 1131.0 60 1011.5 61 1093.0 62 1186.0 63 1557.0 64 1943.0 65 1791.0 66 1276.5 67 695.0 68 316.0 69 198.0 70 135.0 71 99.5 72 83.5 73 65.0 74 57.5 75 57.0 76 47.5 77 33.5 78 36.5 79 28.0 80 17.5 81 16.5 82 12.5 83 7.0 84 2.5 85 1.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045033182856074334 2 0.001552868374347391 3 3.2351424465570645E-4 4 0.01099948431829402 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.002167545439193233 24-25 0.0 26-27 0.0 28-29 0.021707805816397904 30-31 0.017631526333736 32-33 3.2351424465570645E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03739824668219967 50-51 0.013328786879815105 52-53 0.0 54-55 6.793799137769835E-4 56-57 0.0 58-59 0.0 60-61 6.470284893114129E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.6175712232785323E-4 80-81 0.011193592865087443 82-83 0.005111525065560162 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1545527.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.979903172405923 #Duplication Level Percentage of deduplicated Percentage of total 1 70.77038690756407 7.062816088111933 2 10.824761031520422 2.1606013391801335 3 4.310315719915944 1.2904960058178077 4 2.3967166258879775 0.9567599943222979 5 1.4958230171123286 0.7464084436918563 6 1.0748214256446982 0.6435968253336828 7 0.7831989074211154 0.547137448255779 8 0.6211641787693369 0.4959326686628021 9 0.49297572027789843 0.44278649592484437 >10 4.61894719125099 9.955868570082465 >50 0.9700085538873822 6.930042246611377 >100 1.287967005716025 27.360249560955964 >500 0.2211592614199229 15.307812583076322 >1k 0.1263767208113845 22.6114599145994 >5k 0.005377732800484447 3.488031815373333 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 9676 0.6260647662577231 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 6953 0.4498789086182254 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 6420 0.41539229013792706 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 6061 0.3921639673716473 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 5829 0.37715290641962257 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 5810 0.37592355228993085 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5623 0.3638241195398074 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5621 0.3636947138419452 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 4986 0.3226084047706705 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 4357 0.28191031279298256 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 4113 0.2661228176537841 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 4014 0.25971723560960114 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 3987 0.2579702586884603 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 3980 0.2575173387459423 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 3721 0.24075930087277672 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 3550 0.22969511370555157 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 3481 0.22523061712930284 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 3478 0.22503650858250937 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 3462 0.22400126299961112 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 3461 0.22393656015067998 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 3339 0.21604281258108077 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 3309 0.2141017271131465 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 3284 0.21248415588986802 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 3240 0.20963723053689776 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 3206 0.207437333673239 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 3192 0.20653149378820299 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 3138 0.20303753994592136 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 3127 0.2023258086076788 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 3045 0.19702017499532523 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 3012 0.19488498098059756 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2981 0.19287919266373219 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 2803 0.18136208555398903 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 2710 0.17534472060339287 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 2641 0.17088022402714415 No Hit CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT 2603 0.16842151576776077 No Hit ATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTA 2501 0.16182182517678437 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 2496 0.16149831093212866 No Hit CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC 2475 0.16013955110457467 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 2397 0.15509272888794567 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 2354 0.15231050638390659 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 2334 0.15101644940528378 No Hit GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG 2301 0.1488812553905561 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 2299 0.1487518496926938 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 2297 0.14862244399483154 No Hit TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA 2293 0.14836363259910695 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 2261 0.14629314143331046 No Hit CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG 2191 0.14176394200813056 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 2167 0.14021107363378316 No Hit CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC 2165 0.1400816679359209 No Hit CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA 2164 0.14001696508698974 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 2162 0.13988755938912747 No Hit GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG 2142 0.13859350241050464 No Hit ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT 2100 0.1358759827553967 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 2095 0.135552468510741 No Hit CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG 2089 0.13516425141715413 No Hit ATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTT 2074 0.13419370868318703 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 2059 0.1332231659492199 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 2039 0.1319291089705971 No Hit ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC 2016 0.13044094344518084 No Hit TTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGT 2015 0.1303762405962497 No Hit GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG 2012 0.13018213204945625 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 1988 0.12862926367510888 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 1981 0.1281763437325909 No Hit ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA 1970 0.12746461239434834 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 1944 0.12578233832213867 No Hit GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC 1922 0.12435887564565355 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 1906 0.1233236300627553 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1905 0.12325892721382414 No Hit TATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGC 1891 0.12235308732878816 No Hit ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC 1889 0.1222236816309259 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1854 0.11995908191833596 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1834 0.11866502493971312 No Hit ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT 1833 0.11860032209078197 No Hit ACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTAT 1828 0.11827680784612628 No Hit ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT 1815 0.11743567081002144 No Hit GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA 1794 0.11607691098246746 No Hit GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT 1766 0.11426523121239553 No Hit CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG 1762 0.11400641981667095 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 1751 0.1132946884784284 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1733 0.11213003719766784 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1709 0.11057716882332046 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 1709 0.11057716882332046 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 1697 0.10980073463614677 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 1695 0.10967132893828448 No Hit GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 1691 0.10941251754255993 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 1686 0.10908900329790422 No Hit ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT 1685 0.10902430044897307 No Hit ATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATG 1678 0.10857138050645507 No Hit ACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGGTAAAA 1672 0.10818316341286824 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 1664 0.10766554062141911 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 1657 0.10721262067890111 No Hit CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT 1637 0.10591856370027827 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 1631 0.10553034660669143 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 1615 0.10449510102379317 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 1615 0.10449510102379317 No Hit GCCATGAAGTCATTCTAAATTTGTTGAAGCATACGATACTGATATTACTT 1612 0.10430099247699974 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 1595 0.10320104404517036 No Hit TAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTC 1587 0.10268342125372121 No Hit GGCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTG 1561 0.10100114718151156 No Hit GTAAAAGGCCTAGGAGATTTGTTGATCCAATAAATATGATTAGGGAAACA 1558 0.10080703863471813 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 6.470284893114129E-5 0.0 4 0.0 0.0 0.0 1.2940569786228258E-4 0.0 5 0.0 0.0 0.0 1.2940569786228258E-4 0.0 6 0.0 0.0 0.0 1.2940569786228258E-4 0.0 7 0.0 0.0 0.0 1.9410854679342387E-4 0.0 8 0.0 0.0 0.0 1.9410854679342387E-4 0.0 9 0.0 0.0 0.0 3.2351424465570645E-4 0.0 10-11 0.0 0.0 0.0 0.0016175712232785323 0.0 12-13 0.0 0.0 0.0 0.004982119367697879 0.0 14-15 0.0 0.0 0.0 0.00951131879287777 0.0 16-17 0.0 0.0 0.0 0.02717519655107934 0.0 18-19 0.0 0.0 0.0 0.045874319892179174 0.0 20-21 0.0 0.0 0.0 0.08230202384041171 0.0 22-23 0.0 0.0 0.0 0.16266296221288917 0.0 24-25 0.0 0.0 0.0 0.24069459802384557 0.0 26-27 0.0 0.0 0.0 0.2725607511224327 0.0 28-29 0.0 0.0 0.0 0.29669491377374835 0.0 30-31 0.0 0.0 0.0 0.329499258181837 0.0 32-33 0.0 0.0 0.0 0.3718149213828034 0.0 34-35 0.0 0.0 0.0 0.4256800431179785 0.0 36-37 0.0 0.0 0.0 0.4942650629849883 0.0 38-39 0.0 0.0 0.0 0.5963338071738636 0.0 40-41 0.0 0.0 0.0 0.718072217437806 0.0 42-43 0.0 0.0 0.0 0.8269994636133824 0.0 44-45 0.0 0.0 0.0 0.9448880543659217 0.0 46-47 0.0 0.0 0.0 1.094739852490445 0.0 48-49 0.0 0.0 0.0 1.2473738731190074 0.0 50-51 0.0 0.0 0.0 1.412333786468952 0.0 52-53 0.0 0.0 0.0 1.5818552506685422 0.0 54-55 0.0 6.470284893114129E-5 0.0 1.747559246781195 0.0 56-57 0.0 6.470284893114129E-5 0.0 1.9347769401634523 0.0 58-59 0.0 6.470284893114129E-5 0.0 2.1469699332331302 0.0 60-61 0.0 6.470284893114129E-5 0.0 2.3776032382481835 0.0 62-63 0.0 6.470284893114129E-5 0.0 2.5979811417076504 0.0 64-65 0.0 6.470284893114129E-5 0.0 2.8151239027205603 0.0 66-67 0.0 6.470284893114129E-5 0.0 3.0626446513066417 0.0 68-69 0.0 6.470284893114129E-5 0.0 3.3458813725027126 0.0 70-71 0.0 6.470284893114129E-5 0.0 3.692171019982181 0.0 72-73 0.0 6.470284893114129E-5 0.0 4.024096634998935 0.0 74-75 0.0 6.470284893114129E-5 0.0 4.342305246042288 0.0 76-77 0.0 6.470284893114129E-5 0.0 4.697944455192307 0.0 78-79 0.0 6.470284893114129E-5 0.0 5.121974575662541 0.0 80-81 0.0 6.470284893114129E-5 0.0 5.605563668573891 0.0 82-83 0.0 6.470284893114129E-5 0.0 6.1187543148712376 0.0 84-85 0.0 6.470284893114129E-5 0.0 6.578953327893981 0.0 86-87 0.0 6.470284893114129E-5 0.0 7.047434305579909 0.0 88-89 0.0 6.470284893114129E-5 0.0 7.5543487755309355 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCACAG 20 0.0020714116 71.27428 1 ATGTATT 175 0.0 57.019424 1 GACATAT 780 0.0 56.653915 1 GTTATAT 90 2.2555469E-10 47.516182 1 ACATATA 930 0.0 47.503883 2 ATACGAC 40 5.389167E-4 47.49927 3 GGATGAC 510 0.0 47.494663 8 GATGACC 355 0.0 46.825726 9 CATATAG 970 0.0 46.5199 3 GTATGGT 170 0.0 44.72111 1 AGGGTCT 170 0.0 41.90705 5 CATGGGT 660 0.0 41.741783 4 GGTATCA 3935 0.0 41.055912 1 ATGGGAC 255 0.0 40.97579 5 GGGGAGC 315 0.0 39.201946 7 TGGGGAG 380 0.0 37.495785 6 TGGGTAT 445 0.0 37.35535 6 GTCAACA 130 2.055458E-10 36.53435 9 TAACTTT 1630 0.0 35.83953 6 GATCCTC 80 1.0786782E-5 35.620995 5 >>END_MODULE