##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031645_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2377132 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.718386694554614 33.0 31.0 34.0 30.0 34.0 2 31.96634389676299 34.0 31.0 34.0 30.0 34.0 3 32.08848141373723 34.0 31.0 34.0 30.0 34.0 4 35.19072016194305 37.0 35.0 37.0 33.0 37.0 5 35.00962925071052 37.0 35.0 37.0 32.0 37.0 6 35.20987938406449 37.0 35.0 37.0 33.0 37.0 7 35.32880336472691 37.0 35.0 37.0 33.0 37.0 8 35.375231160911554 37.0 36.0 37.0 33.0 37.0 9 37.201651401773226 39.0 38.0 39.0 34.0 39.0 10-11 37.168491695034184 39.0 38.0 39.0 34.5 39.0 12-13 37.24509955694509 39.0 38.0 39.0 35.0 39.0 14-15 38.63652502259025 41.0 39.0 41.0 35.0 41.0 16-17 38.595296138371786 41.0 39.0 41.0 34.5 41.0 18-19 38.59584427789454 41.0 39.0 41.0 34.0 41.0 20-21 38.6311944393496 41.0 39.0 41.0 34.5 41.0 22-23 38.54416793009391 41.0 39.0 41.0 34.0 41.0 24-25 38.46057497017414 41.0 39.0 41.0 34.0 41.0 26-27 38.358610922742194 41.0 39.0 41.0 34.0 41.0 28-29 38.23130141700167 40.5 38.0 41.0 34.0 41.0 30-31 38.111977374415886 40.0 38.0 41.0 34.0 41.0 32-33 37.97506133441475 40.0 38.0 41.0 33.0 41.0 34-35 37.90023755517153 40.0 38.0 41.0 33.0 41.0 36-37 37.79363135913361 40.0 38.0 41.0 33.0 41.0 38-39 37.6062688146893 40.0 38.0 41.0 32.5 41.0 40-41 37.52759943494934 40.0 38.0 41.0 32.0 41.0 42-43 37.41394062256534 40.0 38.0 41.0 31.0 41.0 44-45 37.2513419532445 40.0 38.0 41.0 31.0 41.0 46-47 37.09171219772398 40.0 38.0 41.0 30.0 41.0 48-49 37.188000708416695 40.0 38.0 41.0 30.5 41.0 50-51 36.970549595058245 39.5 37.5 40.5 30.5 41.0 52-53 37.15581212991117 40.0 38.0 41.0 31.0 41.0 54-55 37.469175249838884 40.0 38.0 41.0 31.0 41.0 56-57 37.4410741599541 40.5 38.0 41.0 31.0 41.0 58-59 37.32687099412233 40.0 38.0 41.0 31.0 41.0 60-61 37.09533799553411 40.0 38.0 41.0 30.0 41.0 62-63 36.80003676699485 40.0 37.0 41.0 29.0 41.0 64-65 36.58932970487125 40.0 37.0 41.0 28.0 41.0 66-67 36.35960140202563 39.0 36.0 41.0 28.0 41.0 68-69 36.005582777902106 39.0 36.0 41.0 28.0 41.0 70-71 35.638015684446636 38.5 35.0 40.5 28.0 41.0 72-73 35.211011630822355 37.0 35.0 40.0 27.0 41.0 74-75 34.657686237028486 37.0 35.0 39.0 26.0 41.0 76-77 34.166395261180284 36.0 34.5 39.0 26.0 41.0 78-79 33.69813666216264 36.0 34.0 38.0 25.0 39.5 80-81 33.200598662590046 35.5 34.0 37.0 23.5 39.0 82-83 32.7261222767604 35.0 34.0 37.0 22.0 39.0 84-85 32.347298761701076 35.0 33.5 36.0 20.5 37.5 86-87 31.99150909583481 35.0 33.0 36.0 20.0 37.0 88-89 31.645213012992127 35.0 33.0 35.5 19.5 37.0 90-91 31.33218243665055 35.0 32.0 35.0 18.0 36.0 92-93 31.040670858833252 35.0 32.0 35.0 16.0 36.0 94-95 30.756888342759257 35.0 31.5 35.0 14.0 36.0 96-97 30.534574647095745 35.0 31.0 35.0 11.0 35.5 98-99 30.340162220692832 35.0 31.0 35.0 10.0 35.0 100-101 29.81781743714695 34.5 30.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18114037552162543 1101 2 0.2130022460082479 1101 3 0.24349736263974364 1101 4 0.1699381834812428 1101 5 0.15927769098657762 1101 6 0.16557802555899315 1101 7 0.23179858906543416 1101 8 0.2429337233511646 1101 9 0.18393293856631487 1101 10-11 0.2652155381144965 1101 12-13 0.18535079867942983 1101 14-15 0.3187856498781585 1101 16-17 0.2576325029612647 1101 18-19 0.37075994366554 1101 20-21 0.07426580436570873 1101 22-23 0.13689291592749697 1101 24-25 0.339924454978636 1101 26-27 0.26856558418051435 1101 28-29 0.3785250270308751 1101 30-31 0.2801090477630126 1101 32-33 0.4907286372813857 1101 34-35 0.468779579969997 1101 36-37 0.4544863974490241 1101 38-39 0.4072386270943156 1101 40-41 0.35518736904634807 1101 42-43 0.31436655028694105 1101 44-45 0.31322706734740535 1101 46-47 0.5036582575470163 1101 48-49 0.27117409229003897 1101 50-51 0.3254196015672193 1101 52-53 0.41866582083262927 1101 54-55 0.27047716615065553 1101 56-57 0.40236678150819927 1101 58-59 0.40578525702114376 1101 60-61 0.3003295646484929 1101 62-63 0.11242590397561969 1101 64-65 0.3420149081220458 1101 66-67 0.40939789469958043 1101 68-69 0.2903101220598714 1101 70-71 0.4032896779362005 1101 72-73 0.5215107030229333 1101 74-75 0.37812320147259726 1101 76-77 0.3122481976039566 1101 78-79 0.3415528357771862 1101 80-81 0.4033342872678176 1101 82-83 0.4798165334885667 1101 84-85 0.4241075190396799 1101 86-87 0.45772835285388425 1101 88-89 0.3931355389154412 1101 90-91 0.346015588093767 1101 92-93 0.22839881756761216 1101 94-95 0.20570707864270332 1101 96-97 0.34074203431637073 1101 98-99 0.24003240363753164 1101 100-101 0.4110809407974614 1102 1 0.15461816115783833 1102 2 0.1575002706837303 1102 3 0.24291155211372484 1102 4 0.1212105353676236 1102 5 0.21912502304827797 1102 6 0.12580720606722195 1102 7 0.09560657180545462 1102 8 0.2608455945871526 1102 9 0.30902491796292963 1102 10-11 0.3381364465494485 1102 12-13 0.44226711350258086 1102 14-15 0.420045142509089 1102 16-17 0.38055984102394547 1102 18-19 0.5940948486856499 1102 20-21 0.49694149394327525 1102 22-23 0.3713376818533263 1102 24-25 0.42547333086538686 1102 26-27 0.42753277164702297 1102 28-29 0.37377246053780766 1102 30-31 0.534195433383502 1102 32-33 0.4525679037323016 1102 34-35 0.3484624921627173 1102 36-37 0.4122553523487582 1102 38-39 0.49994760785543235 1102 40-41 0.5087608132550372 1102 42-43 0.41585911692288846 1102 44-45 0.15351243565791606 1102 46-47 -0.11924586241554636 1102 48-49 0.34597298782287567 1102 50-51 0.26831111231541627 1102 52-53 0.375231899545561 1102 54-55 0.4531661079053748 1102 56-57 0.5751165904053721 1102 58-59 0.5430917430867055 1102 60-61 0.3677486763597031 1102 62-63 0.17279028526682794 1102 64-65 0.41067708119334156 1102 66-67 0.29159461273183496 1102 68-69 0.41372146820960154 1102 70-71 0.4351636643768231 1102 72-73 0.3569979616439909 1102 74-75 0.3721659727850337 1102 76-77 0.340593032826888 1102 78-79 0.3183668837993636 1102 80-81 0.2964068991969171 1102 82-83 0.024203289884262347 1102 84-85 0.1843859933604648 1102 86-87 0.38691939852567714 1102 88-89 -0.03421859783559711 1102 90-91 0.12890313390486696 1102 92-93 0.05650629236704319 1102 94-95 0.15997367244025895 1102 96-97 0.22667686646736485 1102 98-99 0.26351661890146616 1102 100-101 0.16319646691961154 1103 1 0.18551021724371353 1103 2 0.14851088724574524 1103 3 0.1511562254385268 1103 4 0.19954319519051467 1103 5 0.2851952650932148 1103 6 0.2624013486771162 1103 7 0.32527532918435753 1103 8 0.29109753276908634 1103 9 0.34266138759100073 1103 10-11 0.376472773891372 1103 12-13 0.3660127144606591 1103 14-15 0.4946975488656733 1103 16-17 0.4156208701252524 1103 18-19 0.43517949479836204 1103 20-21 0.24514508879109087 1103 22-23 0.3147410149942331 1103 24-25 0.439173891520376 1103 26-27 0.3021333346791124 1103 28-29 0.2746518161411373 1103 30-31 0.39161355658723807 1103 32-33 0.37534738509530996 1103 34-35 0.3785709556420329 1103 36-37 0.3883686537837292 1103 38-39 0.35504693083595384 1103 40-41 0.16157778481252194 1103 42-43 0.2891918956005668 1103 44-45 0.3457880632960624 1103 46-47 0.5201195775565637 1103 48-49 0.3481197315979969 1103 50-51 0.43249201608719545 1103 52-53 0.3871926525641882 1103 54-55 0.3304793898708027 1103 56-57 0.3094789797713844 1103 58-59 0.3685622665344894 1103 60-61 0.4912246467524142 1103 62-63 0.5264076022955564 1103 64-65 0.5313064795277853 1103 66-67 0.20812516237294432 1103 68-69 0.3142400146495632 1103 70-71 0.3139711207599518 1103 72-73 0.2820710573356777 1103 74-75 0.20548289283878063 1103 76-77 -0.145906195748978 1103 78-79 0.06562275324473887 1103 80-81 0.27914154400788505 1103 82-83 0.016237189532340324 1103 84-85 -0.039475009932374405 1103 86-87 0.12089352893313787 1103 88-89 0.2378793362418783 1103 90-91 0.2598030004479206 1103 92-93 0.020362269800482835 1103 94-95 -0.03492694798170959 1103 96-97 0.13737645704093637 1103 98-99 -0.006085930703424225 1103 100-101 0.0782308612299758 1104 1 0.06112881400726877 1104 2 0.2496779387694552 1104 3 0.21526799842161637 1104 4 0.14942072119983862 1104 5 0.104070614798907 1104 6 0.22756227891166247 1104 7 0.24570874730943615 1104 8 0.321917966562161 1104 9 0.3736837575069103 1104 10-11 0.4024966891274815 1104 12-13 0.2735998884637141 1104 14-15 0.385566072380513 1104 16-17 0.35483796324137984 1104 18-19 0.461862648451401 1104 20-21 0.3210715979690093 1104 22-23 0.3531063340452718 1104 24-25 0.29545107857696706 1104 26-27 0.383950004532835 1104 28-29 0.44394646451249287 1104 30-31 0.4123278105457473 1104 32-33 0.3107260849587732 1104 34-35 0.3601845913117714 1104 36-37 0.3727930581659038 1104 38-39 0.3383711315143927 1104 40-41 0.36273615897498246 1104 42-43 0.3138996104710543 1104 44-45 0.4493229095969653 1104 46-47 0.582001884684793 1104 48-49 0.5086013679983523 1104 50-51 0.5281623342088935 1104 52-53 0.45077775792717745 1104 54-55 0.3782967389155161 1104 56-57 0.3754262556959347 1104 58-59 0.38752685379473206 1104 60-61 0.4331690827158923 1104 62-63 0.5209018969305319 1104 64-65 0.6183287626700817 1104 66-67 0.3329938637201266 1104 68-69 0.3393485218381116 1104 70-71 0.3054490949924755 1104 72-73 0.2616724290015213 1104 74-75 0.16517962977599865 1104 76-77 0.2422786395445371 1104 78-79 0.26449635676538463 1104 80-81 0.23521186705747965 1104 82-83 0.250503446327599 1104 84-85 0.11933315450040283 1104 86-87 0.1773662780015286 1104 88-89 0.22769348001932954 1104 90-91 0.2894343143664635 1104 92-93 0.20151745155102674 1104 94-95 0.10411745493281188 1104 96-97 0.34269768813352997 1104 98-99 0.13162968431526778 1104 100-101 0.18187096070895947 1105 1 0.19907462072043458 1105 2 0.26355144986309753 1105 3 0.31570096627223876 1105 4 0.2249331324092907 1105 5 0.17455624825077365 1105 6 0.2523533036352035 1105 7 0.16371949302023125 1105 8 0.24999907727362825 1105 9 0.2943894592596692 1105 10-11 0.3452100861638243 1105 12-13 0.3938043956306174 1105 14-15 0.3792662232950903 1105 16-17 0.42423048573951405 1105 18-19 0.36995765170823347 1105 20-21 0.2835083306499868 1105 22-23 0.27356570267605207 1105 24-25 0.31996857836376336 1105 26-27 0.3691244091247867 1105 28-29 0.3266831229103744 1105 30-31 0.22126860284107153 1105 32-33 0.21056318126846918 1105 34-35 0.2649841938168791 1105 36-37 0.20336246712708572 1105 38-39 0.22792650826316674 1105 40-41 0.25150890585503305 1105 42-43 0.18541816208667683 1105 44-45 0.19202258712676468 1105 46-47 0.07347663275881189 1105 48-49 0.1779747326269927 1105 50-51 -0.02331696231414071 1105 52-53 0.058228809570614715 1105 54-55 0.17358958858061868 1105 56-57 0.14123304302010098 1105 58-59 0.1310101499865155 1105 60-61 0.25989653544849034 1105 62-63 0.34197320499277595 1105 64-65 0.38139362627223505 1105 66-67 0.17246092887048547 1105 68-69 0.19441565472079958 1105 70-71 0.024826352994587353 1105 72-73 0.09375011848829473 1105 74-75 0.1428377223690731 1105 76-77 0.24143631128247023 1105 78-79 0.24687222922094776 1105 80-81 0.20593345130204455 1105 82-83 0.1292070641985461 1105 84-85 0.15656159170028872 1105 86-87 0.13367335763770427 1105 88-89 0.18249808829033753 1105 90-91 0.15454548562916415 1105 92-93 0.1635336998963446 1105 94-95 -0.04961752622449822 1105 96-97 0.10574104548217989 1105 98-99 -0.108335485441998 1105 100-101 0.025491328367014177 1106 1 0.08447306387219555 1106 2 0.024644392926997938 1106 3 0.15879864241038177 1106 4 0.22572867227414406 1106 5 0.14638167677199476 1106 6 0.05600169601025584 1106 7 0.1338563961656547 1106 8 0.17595590451642096 1106 9 0.1851375999191518 1106 10-11 0.10999991621587668 1106 12-13 0.23910829140256595 1106 14-15 0.23742575573855618 1106 16-17 0.296345512679693 1106 18-19 0.3235841359286553 1106 20-21 0.28861464225240496 1106 22-23 0.30177313638677106 1106 24-25 0.3105088197642374 1106 26-27 0.2944486384027556 1106 28-29 0.3473494731259237 1106 30-31 0.2813501194330783 1106 32-33 0.2730487755157611 1106 34-35 0.28623567432889274 1106 36-37 0.22509957839997696 1106 38-39 0.3195805282954325 1106 40-41 0.41170190300469045 1106 42-43 0.30596070840758927 1106 44-45 0.4283432266623137 1106 46-47 0.6060222234003803 1106 48-49 0.3915447805282568 1106 50-51 0.24317987811919295 1106 52-53 0.2193344182864081 1106 54-55 0.356746211347307 1106 56-57 0.31018314035163996 1106 58-59 0.37611344921767653 1106 60-61 0.3993091915155631 1106 62-63 0.47305631220809374 1106 64-65 0.5973882907237424 1106 66-67 0.5306754599104622 1106 68-69 0.4524374624630525 1106 70-71 0.4540308359507321 1106 72-73 0.4528866177021129 1106 74-75 0.4151855234521591 1106 76-77 0.22662491539070828 1106 78-79 0.28951417010087965 1106 80-81 0.2999115614042154 1106 82-83 0.3263207789315601 1106 84-85 0.3705130925229838 1106 86-87 0.31735383633088077 1106 88-89 0.4032841346215186 1106 90-91 0.296621351742953 1106 92-93 0.3910541075705858 1106 94-95 0.2030499057061803 1106 96-97 0.27476862008971636 1106 98-99 0.2915821307682087 1106 100-101 0.25299644597501114 1107 1 0.026598530174695156 1107 2 -0.03732768279895282 1107 3 0.03898589169093469 1107 4 0.14598056399614023 1107 5 0.07306352803816196 1107 6 0.023336564555435757 1107 7 0.011772982799428178 1107 8 0.04756923556127646 1107 9 -0.007036737908940438 1107 10-11 0.0492819966990794 1107 12-13 0.07954701928633767 1107 14-15 0.007306671056447556 1107 16-17 0.04458752191907678 1107 18-19 0.04146904870309953 1107 20-21 -0.035621311602653805 1107 22-23 0.08359744335871966 1107 24-25 0.11751740113565035 1107 26-27 0.14452357474200284 1107 28-29 0.07708855241619261 1107 30-31 0.050578349670558964 1107 32-33 0.09117674693645483 1107 34-35 0.08807228542611512 1107 36-37 0.08218592831669724 1107 38-39 0.2688449639219499 1107 40-41 0.2547558431143955 1107 42-43 0.275064747474147 1107 44-45 0.1264682849567791 1107 46-47 0.26733222304688553 1107 48-49 0.17950837326469582 1107 50-51 0.08057813697259775 1107 52-53 0.13351506107367328 1107 54-55 0.2107578076340033 1107 56-57 0.11681427170509551 1107 58-59 0.09874198771843368 1107 60-61 0.2297348997693831 1107 62-63 0.08316311405972243 1107 64-65 0.26367551985944004 1107 66-67 0.1860443217426706 1107 68-69 0.26848195237422345 1107 70-71 0.2154046986496425 1107 72-73 0.2496468720981042 1107 74-75 0.2458966882639757 1107 76-77 0.28588967895686324 1107 78-79 0.3035663840555216 1107 80-81 0.2661191906433089 1107 82-83 0.3494506391994534 1107 84-85 0.2886102716883414 1107 86-87 0.21105070851065477 1107 88-89 0.24078748322605392 1107 90-91 0.2979110702387082 1107 92-93 0.22030072372319154 1107 94-95 0.11543763428969811 1107 96-97 0.15124269816405445 1107 98-99 0.06250925874516966 1107 100-101 0.11847336605100622 1108 1 0.09592692096256528 1108 2 0.11190309821474287 1108 3 0.059973642748303746 1108 4 -0.03834370374890028 1108 5 0.07034068979792352 1108 6 0.030921627966243648 1108 7 0.054375729439179565 1108 8 -0.04327713803647981 1108 9 -0.012284657583933267 1108 10-11 -0.04504259900411256 1108 12-13 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-1.376964252197105 2316 74-75 -1.4658142588249703 2316 76-77 -1.0670716623336247 2316 78-79 -1.3626161633544953 2316 80-81 -1.3937837795851777 2316 82-83 -1.5175619274011325 2316 84-85 -1.9850866707141748 2316 86-87 -1.4416672103092694 2316 88-89 -1.2492554718149087 2316 90-91 -1.5298748746336095 2316 92-93 -1.6689267460468784 2316 94-95 -1.517090063927661 2316 96-97 -1.3721380901176907 2316 98-99 -1.0900007864134018 2316 100-101 -1.6457836300525521 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 27.0 7 816.0 8 5223.0 9 6707.0 10 5786.0 11 6284.0 12 7298.0 13 8585.0 14 8993.0 15 8769.0 16 9554.0 17 10403.0 18 11203.0 19 12619.0 20 13758.0 21 13453.0 22 11188.0 23 11088.0 24 11542.0 25 12536.0 26 14756.0 27 17435.0 28 20507.0 29 24468.0 30 30286.0 31 37480.0 32 47742.0 33 62102.0 34 86163.0 35 123573.0 36 196435.0 37 419520.0 38 840172.0 39 280598.0 40 62.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.16368435759976 28.70981365482832 14.793893252401553 19.33260873517036 2 13.222292086928212 24.173619940973005 43.96383720237881 18.64025076971997 3 14.950648014855128 30.600970810350532 36.077133685601616 18.371247489192726 4 9.004405999648329 21.3993107429395 47.552032667394464 22.044250590017704 5 8.8790025230478 43.15672842271131 39.02971898932998 8.93455006491091 6 21.41900846791735 46.52485743480446 21.831739345970576 10.22439475130762 7 22.15844307697236 37.73007596031568 26.913286542914143 13.198194419797819 8 22.266827459084976 37.232535426950655 25.606543874875285 14.894093239089083 9 25.3371212759859 17.20595673652442 26.164075803813798 31.292846183675877 10-11 21.02679761775533 28.417320701942238 35.03790365841706 15.51797802188537 12-13 21.052532857140747 30.526491935798305 31.532717832078887 16.88825737498206 14-15 19.771568190191783 31.170226130123673 29.942027861362615 19.116177818321926 16-17 17.67972352898451 33.271471926044214 31.201644678322438 17.847159866648845 18-19 17.535620715682164 32.25718753268112 33.06821598780059 17.13897576383612 20-21 18.766544397195524 32.12805460710652 31.738613826399227 17.366787169298732 22-23 18.471755039265805 31.345062032735242 32.026618631190864 18.156564296808085 24-25 18.581908787564174 33.15375418781961 30.855459435992614 17.408877588623604 26-27 19.546600693608937 31.406375413733862 32.40827602337607 16.638747869281133 28-29 18.36057063722166 30.053842193029247 33.92760688089681 17.657980288852283 30-31 18.712318037029497 31.64418299025885 31.744177437348874 17.89932153536278 32-33 17.848945704319323 31.037674811495535 32.97370528855781 18.139674195627336 34-35 19.27152131223676 31.85147059565897 30.74246613145589 18.134541960648377 36-37 19.414235305401636 29.331690457240068 32.00352357378555 19.250550663572742 38-39 17.74518621599474 31.90767277542854 31.96946993267517 18.37767107590155 40-41 17.70629481240419 30.98477493046242 32.860186140273235 18.448744116860148 42-43 17.622938061495955 30.488567736246875 31.835043236976325 20.05345096528085 44-45 19.102528187510927 30.19255761247096 32.34837299138314 18.356541208634972 46-47 17.981776374544424 30.34809700352909 32.17172422965672 19.498402392269767 48-49 17.401871673346204 31.05205995434604 31.610913582315753 19.935154789992 50-51 16.501434899146638 30.151950926133473 31.034990070939898 22.31162410377999 52-53 17.212618201443632 31.544177513288325 31.196298382499915 20.04690590276813 54-55 17.8436686506625 29.62864178735612 31.520377673542093 21.007311888439283 56-57 18.07534154410766 29.916393632834897 31.317547313521878 20.690717509535563 58-59 18.654656121472552 29.588996392415122 30.81595422041112 20.940393265701204 60-61 19.005914776747247 29.858532762125545 31.10455154087296 20.03100092025425 62-63 18.27569535707531 29.62401728411877 30.808473637480915 21.291813721325 64-65 17.596489960680127 29.25959476248957 30.304059767720215 22.839855509110084 66-67 18.875606019929567 29.096220962367543 30.45477946401781 21.573393553685076 68-69 18.207951902315475 29.05054577782869 30.51451652368224 22.226985796173597 70-71 18.383799850988876 28.94468419978943 30.57826968149978 22.093246267721913 72-73 19.251531970571346 27.946969990384662 30.81132134250972 21.99017669653427 74-75 17.92548670609113 28.74479591783 30.594916244951264 22.7348011311276 76-77 17.979165173546264 30.126960906243283 30.537059599745366 21.356814320465094 78-79 18.626975817008745 27.895904303507063 30.813509077126028 22.663610802358164 80-81 19.02799601199769 28.34224655218001 30.49718667320106 22.13257076262124 82-83 18.839136520891707 29.178978207546315 30.580614899492563 21.401270372069416 84-85 18.90016688191894 28.052522115117785 30.653298707686293 22.394012295276987 86-87 18.026048655731493 28.189150077093494 31.709896910471556 22.07490435670345 88-89 19.579426649365775 27.179235393530355 30.658453585825846 22.582884371278023 90-91 19.502506085425285 26.97567577755404 31.84258401508979 21.679234121930886 92-93 19.560885939269443 28.145374101287825 31.336567380369228 20.957172579073504 94-95 19.007596399845237 27.101057371174285 31.38974710330257 22.501599125677902 96-97 20.587298462859906 25.725112656590294 30.83379034558547 22.85379853496433 98-99 19.053032926316106 27.203579691477476 29.865972070340085 23.877415311866326 100-101 18.888421405339738 27.18210341077541 31.512497771618875 22.41697741226598 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 170.0 1 110.0 2 109.5 3 225.5 4 191.0 5 123.0 6 222.0 7 390.0 8 530.0 9 575.0 10 480.5 11 360.0 12 365.5 13 414.5 14 458.0 15 501.0 16 543.0 17 626.5 18 721.5 19 815.5 20 974.5 21 1107.5 22 1273.0 23 1563.0 24 1878.0 25 2340.5 26 3061.0 27 5203.5 28 8215.0 29 10772.0 30 16188.5 31 26685.5 32 50009.5 33 84416.0 34 137031.0 35 184945.0 36 208216.0 37 225824.5 38 216382.0 39 194763.5 40 172773.5 41 150130.5 42 130699.0 43 114501.5 44 102004.5 45 77111.0 46 52565.0 47 36962.0 48 31863.0 49 28865.0 50 23780.5 51 18957.5 52 12238.5 53 7540.5 54 5092.5 55 3744.5 56 3004.5 57 2627.0 58 2328.0 59 2013.5 60 1741.0 61 1472.5 62 1227.0 63 979.5 64 774.5 65 655.0 66 529.0 67 445.5 68 373.5 69 293.5 70 227.5 71 189.5 72 176.0 73 132.0 74 83.5 75 66.5 76 63.0 77 57.0 78 42.0 79 24.5 80 16.5 81 13.5 82 13.5 83 11.0 84 7.5 85 4.5 86 2.5 87 1.0 88 0.5 89 2.5 90 2.0 91 0.5 92 1.5 93 1.5 94 0.5 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4914746004849541 2 0.3812577509368432 3 0.20558387165710612 4 0.23528352653533752 5 0.26039782393236893 6 0.20545766915762356 7 0.19809585668780696 8 0.19834826168677214 9 0.19738070919073908 10-11 0.2103164653877025 12-13 0.1978224179389281 14-15 0.20857066414486028 16-17 0.09252746587063738 18-19 0.04002722608588837 20-21 2.3137124905137785E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.13869654693134417 46-47 0.2099799253890823 48-49 0.08798417588926488 50-51 0.06434224098619681 52-53 0.07311331470023541 54-55 0.08241023216211806 56-57 0.11490737577887977 58-59 0.09568252835770164 60-61 0.14014787567539372 62-63 0.24005818776576143 64-65 0.13215505070816427 66-67 0.10598906581544482 68-69 0.11067959204621367 70-71 0.09008755088064103 72-73 0.07403879969644092 74-75 0.1427981281645277 76-77 0.21631108411312455 78-79 0.1403161456747038 80-81 0.08447153965366669 82-83 0.09080269837770893 84-85 0.10091993208622828 86-87 0.0744384409448024 88-89 0.09686041835287229 90-91 0.11827277576508162 92-93 0.16593525306966547 94-95 0.13371154820178266 96-97 0.08853105338702268 98-99 0.07755143593203911 100-101 0.0643001734863693 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2377132.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.569302722729018 #Duplication Level Percentage of deduplicated Percentage of total 1 77.03682536946008 11.223728296056747 2 9.221515080034248 2.6870208952645935 3 3.7753724050589668 1.6501363038102481 4 2.0055108481969808 1.1687557864439544 5 1.2537802774476776 0.9133352204961196 6 0.8471317601499754 0.7405271435797957 7 0.5783764554227658 0.5898579166727282 8 0.4524150902920769 0.527309792543684 9 0.38484921010512846 0.5046286180162284 >10 2.9798011991301454 9.145358888688266 >50 0.5666046897778549 5.881017591114595 >100 0.683853125322069 21.792535474030466 >500 0.12468862467770755 12.858982895678626 >1k 0.08302655437966684 23.37096262842211 >5k 0.005356551895462377 4.977556114088117 >10k+ 8.927586492437295E-4 1.9682864350937432 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 20898 0.8791266113955809 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 12852 0.5406515077833288 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 11648 0.49000223799099085 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 9394 0.3951820933797534 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 9175 0.38596931091752584 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 8535 0.35904611102791095 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 7883 0.33161810114036583 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 6889 0.28980300631180766 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 6754 0.284123893835092 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 6268 0.2636790889189157 No Hit AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT 5872 0.24702035898721653 No Hit GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC 5758 0.24222466400687886 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 5720 0.240626099013433 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 5682 0.2390275340199871 No Hit TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT 5528 0.23254913904654853 No Hit ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC 5524 0.23238086904723843 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 5364 0.22565006907483473 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 5263 0.22140125159225485 No Hit GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG 5144 0.21639521911277962 No Hit AACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGACCCAGA 5027 0.21147332163295937 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 5026 0.21143125413313185 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 4772 0.20074610917694097 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 4545 0.19119678671609316 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 4487 0.18875687172609684 No Hit CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAA 4446 0.18703210423316838 No Hit TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT 4434 0.1865272942352381 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 4431 0.18640109173575553 No Hit GTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAAATCCT 4410 0.18551767423937754 No Hit GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAAT 4402 0.18518113424075736 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 4391 0.18471839174265461 No Hit GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT 4348 0.1829094892500711 No Hit ATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTA 4308 0.18122678925697017 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 4230 0.17794552427042334 No Hit GATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGA 4036 0.16978442930388385 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 4030 0.1695320243049187 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 4008 0.1686065393087132 No Hit GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCT 3975 0.16721831181440494 No Hit CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA 3970 0.1670079743152673 No Hit CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA 3889 0.1636005068292379 No Hit CCCTAATTGTCGATATGAACTCTTAAATAGGATTGCGCTGTTATCCCTAG 3753 0.15787932685269476 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 3686 0.1550608043642507 No Hit ACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTC 3681 0.15485046686511308 No Hit GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG 3543 0.14904515188891487 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 3518 0.1479934643932268 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 3508 0.14757278939495155 No Hit GGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTA 3451 0.14517494190478275 No Hit CTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGTAAATAGATAGAAAC 3427 0.14416532190892217 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 3399 0.14298743191375152 No Hit GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC 3329 0.1400427069258249 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 3320 0.1396640994273772 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 3121 0.13129266696170006 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 3109 0.1307878569637698 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 3072 0.1292313594701514 No Hit GATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGT 3059 0.12868448197239363 No Hit CTCTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGAT 3052 0.12839000947360096 No Hit TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT 3047 0.12817967197446334 No Hit GTCGTAAACCCTAATTGTCGATATGAACTCTTAAATAGGATTGCGCTGTT 3010 0.12662317448084498 No Hit ATCTTATTGACCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGG 3007 0.12649697198136242 No Hit GTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTT 2991 0.12582389198412203 No Hit GGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGG 2922 0.12292123449602294 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 2883 0.12128060200274954 No Hit AGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGTTCTTA 2871 0.12077579200481925 No Hit CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA 2851 0.11993444200826878 No Hit GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG 2840 0.11947169951016604 No Hit AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGACAATTAGGGTT 2808 0.1181255395156853 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 2751 0.11572769202551647 No Hit ATTAAGTTGAGAGCGCTTATTTGTAAGGTGGCTCTATTTCTCTTGTCCTT 2747 0.11555942202620637 No Hit ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCTCGATGTTGGATC 2729 0.11480220702931095 No Hit AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG 2676 0.11257262953845222 No Hit AGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAAT 2671 0.1123622920393146 No Hit CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT 2655 0.11168921204207424 No Hit ACGTAGGACTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACC 2648 0.11139473954328158 No Hit GATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTAT 2638 0.11097406454500633 No Hit CTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAA 2628 0.1105533895467311 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 2610 0.10979617454983567 No Hit CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA 2607 0.10966997205035312 No Hit GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC 2581 0.1085762170548375 No Hit GCCATGAAGTCATTCTAAATTTGTTGAAGCATACGATACTGATATTACTT 2568 0.1080293395570797 No Hit GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT 2566 0.10794520455742466 No Hit CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT 2563 0.10781900205794209 No Hit TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA 2558 0.10760866455880448 No Hit GTACATGGGAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGAC 2556 0.10752452955914943 No Hit GATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAGATCAT 2498 0.10508461456915306 No Hit TTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGACC 2473 0.104032927073465 No Hit GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC 2471 0.10394879207380994 No Hit ATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATA 2467 0.10378052207449986 No Hit ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT 2461 0.10352811707553472 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 2455 0.10327571207656959 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 2444 0.10281296957846682 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 2443 0.10277090207863929 No Hit ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA 2399 0.10091993208622828 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 2382 0.10020478458916038 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.2620249948256976E-4 0.0 2 0.0 0.0 0.0 2.9447249879266277E-4 0.0 3 0.0 0.0 0.0 5.04809997930279E-4 0.0 4 0.0 0.0 0.0 8.41349996550465E-4 0.0 5 0.0 0.0 0.0 9.254849962055115E-4 0.0 6 0.0 0.0 0.0 9.254849962055115E-4 0.0 7 0.0 0.0 0.0 0.0015564974936183604 0.0 8 0.0 0.0 0.0 0.001850969992411023 0.0 9 0.0 0.0 0.0 0.0030709274874091974 0.0 10-11 0.0 0.0 0.0 0.006036686225249587 0.0 12-13 0.0 0.0 0.0 0.01228370994963679 0.0 14-15 0.0 0.0 0.0 0.025829444894099277 0.0 16-17 0.0 0.0 0.0 0.063795363488439 0.0 18-19 0.0 0.0 0.0 0.10460083832113656 0.0 20-21 0.0 0.0 0.0 0.16488356557397738 0.0 22-23 0.0 0.0 0.0 0.2785499501079452 0.0 24-25 0.0 0.0 0.0 0.43131807573159586 0.0 26-27 0.0 0.0 0.0 0.5555644364721858 0.0 28-29 0.0 0.0 0.0 0.6868360696839722 0.0 30-31 0.0 0.0 0.0 0.8882131913583259 0.0 32-33 0.0 0.0 0.0 1.2306636737042789 0.0 34-35 0.0 0.0 0.0 1.7656991702606335 0.0 36-37 0.0 0.0 0.0 2.3965223639242583 0.0 38-39 0.0 0.0 0.0 3.3203667276364968 0.0 40-41 4.206749982752325E-5 0.0 0.0 4.318965038542244 0.0 42-43 4.206749982752325E-5 0.0 0.0 5.133286666453525 0.0 44-45 4.206749982752325E-5 0.0 0.0 6.054964553924645 0.0 46-47 4.206749982752325E-5 0.0 0.0 7.01725861247924 0.0 48-49 4.206749982752325E-5 2.1033749913761626E-5 0.0 8.014636965889988 0.0 50-51 4.206749982752325E-5 4.206749982752325E-5 0.0 9.142361467516317 0.0 52-53 4.206749982752325E-5 4.206749982752325E-5 0.0 10.07836333867871 0.0 54-55 4.206749982752325E-5 4.206749982752325E-5 0.0 10.854845250495135 0.0 56-57 4.206749982752325E-5 4.206749982752325E-5 0.0 11.738431016872433 0.0 58-59 4.206749982752325E-5 4.206749982752325E-5 0.0 12.737155530277663 0.0 60-61 4.206749982752325E-5 4.206749982752325E-5 0.0 13.710303003787757 0.0 62-63 4.206749982752325E-5 4.206749982752325E-5 0.0 14.60867549635443 0.0 64-65 4.206749982752325E-5 4.206749982752325E-5 0.0 15.495016683970432 0.0 66-67 4.206749982752325E-5 4.206749982752325E-5 0.0 16.355023616694403 0.0 68-69 4.206749982752325E-5 4.206749982752325E-5 0.0 17.269949670443204 0.0 70-71 4.206749982752325E-5 4.206749982752325E-5 0.0 18.304452592451746 0.0 72-73 4.206749982752325E-5 4.206749982752325E-5 0.0 19.258017644792126 0.0 74-75 4.206749982752325E-5 4.206749982752325E-5 0.0 20.09190066012321 0.0 76-77 4.206749982752325E-5 4.206749982752325E-5 0.0 21.01336821009519 0.0 78-79 4.206749982752325E-5 4.206749982752325E-5 0.0 21.941882066288283 0.0 80-81 4.206749982752325E-5 4.206749982752325E-5 0.0 23.05019241674421 0.0 82-83 4.206749982752325E-5 4.206749982752325E-5 0.0 24.058171780111493 0.0 84-85 4.206749982752325E-5 4.206749982752325E-5 0.0 24.99417365127389 0.0 86-87 4.206749982752325E-5 6.310124974128488E-5 0.0 25.96769973228243 0.0 88-89 4.206749982752325E-5 8.41349996550465E-5 0.0 27.003422611785968 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTATAT 615 0.0 58.853188 1 TAACTTT 4805 0.0 50.81932 6 GACATAT 605 0.0 49.592583 1 CGCAGCG 40 5.3864595E-4 47.505165 9 AACTTTA 5620 0.0 43.616837 7 ACTTTAT 5675 0.0 43.277832 8 GAGTGAT 390 0.0 42.63284 7 TATGGGC 420 0.0 41.866554 4 GGTAAGG 60 8.166366E-5 39.68718 1 AACGTGC 60 8.2881146E-5 39.587635 8 ACATATA 770 0.0 39.543156 2 ATTGAAC 580 0.0 37.67651 8 GTGCCTT 190 0.0 37.504078 9 CATGGGT 975 0.0 36.55707 4 AGGAGTG 520 0.0 36.55707 5 CTCTAAT 850 0.0 35.858536 1 TAAGTTG 825 0.0 35.712894 3 GGAGTGA 480 0.0 35.630375 6 ATCTATA 6940 0.0 35.40966 1 TACCTGG 310 0.0 35.297848 2 >>END_MODULE