FastQCFastQC Report
Fri 10 Feb 2017
SRR2031645_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031645_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2377132
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT331791.395757576777394No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC237620.9996079309016075No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA129440.5445217177674609No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT120740.5079229929175157TruSeq Adapter, Index 3 (95% over 21bp)
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC115890.48752025550116695No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA94390.3970751308719919No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC86470.3637576710085935No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT85020.35765788353360267No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC81150.3413777611003512No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC70190.2952717812893857No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC67620.2844604338337122No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC67500.283955623835782No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT63510.26717069140460015No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG63180.2657824639102919No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC62940.26477284391443134No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG61390.25825238144116525No Hit
TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT57410.241509516509811No Hit
AACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGACCCAGA56750.23873306152119445No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC56210.23646141653050817No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA55370.23292774654499623No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT53260.22405150408138885No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC50970.214418046620886No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC49720.20915960914244558No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG49530.20836032664572268No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA48300.2031860241669373No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT47170.19843239668642718No Hit
GATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGA47060.1979696541883244No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAA46880.197212439191429No Hit
GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG46500.19561387419798315No Hit
TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT45890.19304775670850421No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC43580.18333016424834633No Hit
ATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTA42720.17971235926317933No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC41320.17382290928732608No Hit
GTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAAATCCT40610.17083611679957192No Hit
GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT40590.17075198179991688No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT40470.1702471718019866No Hit
GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCT40110.16873274180819578No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAAT39760.16726037931423246No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT39180.1648204643242361No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG38780.16313776433113517TruSeq Adapter, Index 3 (96% over 28bp)
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC38680.16271708933285994No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA38270.16099232183993148No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG36420.1532098343718397No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA36370.15299949687270206No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA35990.15140093187925618No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC35930.15114852688029104No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT35670.15005477188477545No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA35120.14774105939426166No Hit
CTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGTAAATAGATAGAAAC34620.1456376844028855No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT34440.1448804694059901No Hit
CCCTAATTGTCGATATGAACTCTTAAATAGGATTGCGCTGTTATCCCTAG34270.14416532190892217No Hit
ACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTC33900.14260882441530381No Hit
GGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTA33160.1394958294280671No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC32860.1382338044332414No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT32550.1369297119385882No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT32150.13524701194548724RNA PCR Primer, Index 3 (95% over 23bp)
ATCTTATTGACCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGG32080.1349525394466946No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT31960.1344477294487643No Hit
GATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGT31270.1315450719606652No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT31060.1306616544642872No Hit
AGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGTTCTTA29800.12536114948601929No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT29760.12519287948670918No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGACAATTAGGGTT29640.12468806948877892No Hit
GTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTT29450.12388878699205598No Hit
CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA29160.1226688294970578No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT28980.12191161450016239No Hit
ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT28950.12178541200067981No Hit
GCCATGAAGTCATTCTAAATTTGTTGAAGCATACGATACTGATATTACTT28660.12056545450568164No Hit
ATTAAGTTGAGAGCGCTTATTTGTAAGGTGGCTCTATTTCTCTTGTCCTT28630.12043925200619908No Hit
TTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGACC28490.11985030700861375No Hit
AGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAAT28490.11985030700861375No Hit
GGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGG28380.11938756451051098No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCTCGATGTTGGATC28260.11888275451258071No Hit
GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG27960.117620729517755No Hit
GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC27860.11720005451947978No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC27810.11698971702034217No Hit
CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT27780.1168635145208596No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT27640.11627456952327427No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA27370.11513874702793114No Hit
CTCTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGAT27230.11454980203034582No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA27080.11391878953293297No Hit
CTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAA26950.11337191203517516No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT26020.10945963455121549No Hit
GATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAGATCAT25810.1085762170548375No Hit
GTCGTAAACCCTAATTGTCGATATGAACTCTTAAATAGGATTGCGCTGTT25740.10828174455604483No Hit
AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG25680.1080293395570797No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA25630.10781900205794209No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACC25530.10739832705966687No Hit
CGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTATAGGCAATA25080.10550528956742831No Hit
ATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATA24740.10407499457329253No Hit
GATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTAT24380.10256056457950168No Hit
ACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGT24380.10256056457950168No Hit
GCTATACTATGAGCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGTCT24360.10247642957984662No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA24150.10159301208346865No Hit
AAACTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAA24130.1015088770838136No Hit
ACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGACCCAGAT24070.10125647208484848No Hit
GTACATGGGAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGAC23950.10075166208691817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAT8550.061.686471
GACATAT7300.059.231351
AACTTGC4000.054.6184127
TAACTTT51000.052.6161966
ACATATA8750.049.9441872
GTGCGTA405.3925556E-447.494275
AACTTTA59400.045.4153947
ATACTCA1900.044.9945686
CATATAG9700.044.5611653
TGGGCGA1400.044.1018186
GGCGATA1300.043.8408628
ACTTTAT62550.042.9004978
GGTAAGG1006.348273E-1042.7637331
GGGCGAT1350.042.217137
GCGATAT1350.042.217139
AAACTTG5450.041.8298156
CGCAGAA3300.041.7373859
TATGGGC3700.039.796684
ACTTGCT5800.039.3056038
ATGGGCG1357.2759576E-1238.6990365
Produced by FastQC (version 0.11.5)